Glycoside Hydrolase Family 5 / Subf 4

Activities in Familyendo-β-1,4-glucanase / cellulase (EC 3.2.1.4); endo-β-1,4-xylanase (EC 3.2.1.8); β-glucosidase (EC 3.2.1.21); β-mannosidase (EC 3.2.1.25); β-glucosylceramidase (EC 3.2.1.45); glucan β-1,3-glucosidase (EC 3.2.1.58); licheninase (EC 3.2.1.73); exo-β-1,4-glucanase / cellodextrinase (EC 3.2.1.74); glucan endo-1,6-β-glucosidase (EC 3.2.1.75); mannan endo-β-1,4-mannosidase (EC 3.2.1.78); cellulose β-1,4-cellobiosidase (EC 3.2.1.91); steryl β-glucosidase (EC 3.2.1.104); endoglycoceramidase (EC 3.2.1.123); chitosanase (EC 3.2.1.132); β-primeverosidase (EC 3.2.1.149); xyloglucan-specific endo-β-1,4-glucanase (EC 3.2.1.151); endo-β-1,6-galactanase (EC 3.2.1.164); hesperidin 6-O-α-L-rhamnosyl-β-glucosidase (EC 3.2.1.168); β-1,3-mannanase (EC 3.2.1.-); arabinoxylan-specific endo-β-1,4-xylanase (EC 3.2.1.-); mannan transglycosylase (EC 2.4.1.-)
Activities in Sub Family
Mechanism Retaining
ClanGH-A
3D Structure Status( β / α ) 8
Catalytic Nucleophile/BaseGlu (experimental)
Catalytic Proton DonorGlu (experimental)
NoteOnce known as cellulase family A; many members have been assigned to subfamilies as described by Aspeborg et al. (2012) BMC Evol Biol. 12(1):186 (PMID: 22992189).
External resourcesCAZypedia; HOMSTRAD; PROSITE;
Commercial Enzyme Provider(s)MEGAZYME; NZYTech; PROZOMIX;
Statistics GenBank accession (601); Uniprot accession (185); PDB accession (34); 3D entries (16); cryst (0)
Bacteria
Protein Name EC# OrganismGenBankUniprot
PDB/3D Carbohydrate Ligands Resolution (Å)
Subf
 β-glucanase (CelB;BhCel5B;BH0603) (Cel5B) 3.2.1.4 Bacillus halodurans C-125 BAB04322.1
NP_241469.1
Q9KF82
4V2X[A] 1.64
4
 endoglucanase (BlCel5B;BLi01882;BL01230) (Cel5B) 3.2.1.4 Bacillus licheniformis DSM 13 = ATCC 14580 AAU40777.1
AAU23417.1
Q65JI7
4YZP[A] 1.70
4YZT[A] β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp
1.67
4
 cellulase (CelB) 3.2.1.4 Bacillus sp. BG-CS10 ADD62401.1 D4P8C6
5E09[A] 2.35
5E0C[A] 2.35
4
 endo-xyloglucanase (CelA;BoGH5A;BACOVA_02653;Bovatus_03072) 3.2.1.151 Bacteroides ovatus ATCC 8483 ALJ47680.1  
3ZMR[A,B] α-D-Xylp-(1-6)-β-D-Glcp-(1-4)[α-D-Xylp-(1-6)]-β-D-Glcp-(1-4)[α-D-Xylp-(1-6)]-β-D-Glcp-(1-1)-<non_carb>
α-D-Xylp-(1-6)-β-D-Glcp-(1-4)[α-D-Xylp-(1-6)]-β-D-Glcp-(1-4)[α-D-Xylp-(1-6)]-β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-GLU
1.43
4
 endo-cellulase (AC2aCel5A) (Cel5A) 3.2.1.4 Bacteroidetes bacterium AC2a AIJ19564.1  
4YHE[A,B] 1.85
4
 bpr_I1710 (Cel5C)   Butyrivibrio proteoclasticus B316 ADL34447.1 E0RXM0
4NF7[A] 2.11
4
 β-1,3-1,4-glucanase / lichenase   Caldicellulosiruptor sp. F32 AGM71677.1  
4X0V[A,B,C,D,E,F,G,H] 2.80
5H4R[A] β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp
1.70
4
 endo-β-1,4-glucanase A (CelA;Cca;CelCcaCcel_1099) (Cel5A) 3.2.1.4 [Clostridium] cellulolyticum H10 AAA23221.1
AAA51444.1
ACL75458.1
P17901
1EDG[A] 1.60
4
 endo-β-1,4-glucanase / xylanase D (EngD) 3.2.1.4
3.2.1.73
3.2.1.8
Clostridium cellulovorans ATCC 35269 AAA23233.1 P28623
3ICG[A,B,C,D] 2.10
3NDY[A,B,C,D] 2.10
3NDZ[A,B,C,D] β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp
2.08
4
 xyloglucanase (XG5;PpXG5) 3.2.1.151 Paenibacillus pabuli AAR65335.1 H9KVH3
2JEP[A,B] 1.4
2JEQ[A] α-D-Xylp-(1-6)[β-D-Glcp-(1-4)]-β-D-Glcp-(1-4)[β-D-Galp-(1-2)-α-D-Xylp-(1-6)]-β-D-Glcp-(1-4)-β-D-Glcp
1.94
4
 mixed-linkage β-glucanase/xyloglucanase (Orf4;CMCase;PBR_0368) (Cel5A) 3.2.1.151
3.2.1.73
Prevotella bryantii B14 AAC97596.1
AAA22909.1
O06842
Q44878
3L55[A,B] 1.60
3VDH[A,B] 1.62
5D9M[A,B] α-D-Xylp-(1-6)-β-D-Glcp-(1-4)[α-D-Xylp-(1-6)]-β-D-Glcp-(1-4)[α-D-Xylp-(1-6)]-β-D-Glcp-(1-4)-α-D-Glcp
α-D-Xylp-(1-6)-β-D-Glcp-(1-4)[α-D-Xylp-(1-6)]-β-D-Glcp-(1-4)[α-D-Xylp-(1-6)]-β-D-Glcp-(1-4)-β-D-Glcp-(1-4)[α-D-Xylp-(1-6)]-β-D-Glcp-(1-4)[α-D-Xylp-(1-6)]-β-D-Glcp-(1-4)[α-D-Xylp-(1-6)]-β-D-Glcp-(1-4)-α-D-Glcp
1.90
5D9N[A,B] α-D-Xylp-(1-6)-β-D-Glcp-(1-4)[α-D-Xylp-(1-6)]-β-D-Glcp-(1-4)[α-D-Xylp-(1-6)]-β-D-Glcp-(1-4)-β-D-Glcp
1.86
5D9O[A,B] β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp
1.55
5D9P[A,B] α-D-Xylp-(1-6)-β-D-Glcp-(1-4)[α-D-Xylp-(1-6)]-β-D-Glcp-(1-4)[α-D-Xylp-(1-6)]-β-D-Glcp-(1-4)-β-D-Glcp1N-(1-1)-<non_carb>
1.80
4
 endo-β-1,4-glucanase E (CelE;EgE) (Cel5C) 3.1.1.72
3.2.1.4
Ruminiclostridium thermocellum AAA23224.1 P10477
4IM4[A,B,C,D,E,F] 2.42
4
 xyloglucan-specific endo-β-1,4-glucanase (XEG5A) (Xeg5A) 3.2.1.151 uncultured bacterium ACZ54907.1 D2K7Z0
4W84[A,B] 1.79
4W85[A,B] β-D-Glcp
1.92
4W86[A,B] β-D-Glcp
2.64
4W87[A,B] α-D-Xylp-(1-6)-β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp
2.15
4W88[A,B] β-D-Galp-(1-2)-α-D-Xylp-(1-6)[α-D-Xylp-(1-6)-β-D-Glcp-(1-4)]-β-D-Glcp-(1-4)-β-D-Glcp
β-D-Galp-(1-2)-α-D-Xylp-(1-6)[β-D-Glcp-(1-4)]-β-D-Glcp
1.58
4W89[A,B] β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-α-D-Glcp
2.40
4
 exo-xyloglucanase (XEG5B) (Xeg5B) 3.2.1.- uncultured bacterium ADB44000.1 D2XML9
4W8A[A] 1.72
4W8B[A] α-D-Xylp-(1-6)-β-D-Glcp-(1-4)[α-D-Xylp-(1-6)]-β-D-Glcp-(1-4)-β-D-Glcp
α-D-Xylp-(1-6)-β-D-Glcp-(1-4)[α-D-Xylp-(1-6)]-β-D-Glcp-(1-4)[β-D-Galp-(1-2)-α-D-Xylp-(1-6)]-β-D-Glcp-(1-4)-α-D-Glcp
1.15
4
Eukaryota
Protein Name EC# OrganismGenBankUniprot
PDB/3D Carbohydrate Ligands Resolution (Å)
Subf
 endoglucanase A (CelA;EglA) (partial) 3.2.1.4 Piromyces rhizinflatus 2301 AAF00074.2  
3AYR[A] 2.00
3AYS[A] β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp
2.20
4
unclassified
Protein Name EC# OrganismGenBankUniprot
PDB/3D Carbohydrate Ligands Resolution (Å)
Subf
 SARM_0063 / 2527869_58630 / TW-33 3.2.1.4 uncultured organism ADX05734.1  
4YHG[A,B] β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp
2.40
4
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