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Glycoside Hydrolase Family 5 / Subf 4

Activities in Sub FamilyGlucomannan-specific endo-β-1,4-glucanase (EC 3.2.1.-);
Oligoxyloglucan reducing-end-specific cellobiohydrolase (EC 3.2.1.150);
Xyloglucan-specific endo-β-1,4-glucanase / endo-xyloglucanase (EC 3.2.1.151);
Endo-β-1,4-glucanase (EC 3.2.1.4);
Endo-β-1,3(4)-glucanase / lichenase-laminarinase (EC 3.2.1.6);
Lichenase / endo-β-1,3-1,4-glucanase (EC 3.2.1.73);
Endo-β-1,4-mannanase (EC 3.2.1.78);
Endo-β-1,4-xylanase (EC 3.2.1.8);
Mechanism Retaining e
ClanGH-A
3D Structure Status( β / α ) 8 barrel
Catalytic Nucleophile/BaseGlu (experimental)
Catalytic Proton DonorGlu (experimental)
NoteOnce known as cellulase family A; many members have been assigned to subfamilies as described by Aspeborg et al. (2012) BMC Evol Biol. 12(1):186 (PMID: 22992189).
External resourcesCAZypedia; HOMSTRAD; PROSITE;
Commercial Enzyme Provider(s)MEGAZYME; NZYTech; PROZOMIX;
Statistics GenBank accession (459); Uniprot accession (141); PDB accession (56); 3D entries (28); cryst (0)
Summary
Download GH5_4 (2023) Taxonomic display Structure (28) Characterized (127)
Bacteria
Protein Name EC# OrganismGenBankUniprot
PDB/3D Carbohydrate Ligands Resolution (Å)
Subf
 glucomannanase (AceceDRAFT_0142) 3.2.1.- Acetivibrio cellulolyticus WP_010248927.1 A0A5S8WF61
6MQ4[A] 1.40
4
 endo-β-1,4-glucanase E (CelE;EgE) (Cel5C) 3.2.1.4
3.2.1.8
3.2.1.-		 Acetivibrio thermocellus AAA23224.1 P10477
4IM4[A,B,C,D,E,F] 2.42
4
 endoglucanase (BlCel5B; BLi01882; BL01230) (Cel5B) 3.2.1.4 Bacillus licheniformis DSM 13 = ATCC 14580 AAU40777.1
AAU23417.1		 Q65JI7
4YZP[A] 1.70
4YZT[A] β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp
 1.67
4
 cellulase (CelB) 3.2.1.4 Bacillus sp. BG-CS10 ADD62401.1 D4P8C6
5E09[A] 2.35
5E0C[A] 2.35
5XRC[A,B,C] 2.90
4
 endo-xyloglucanase (CelA; BoGH5A; BoGH5; BACOVA_02653; Bovatus_03072) 3.2.1.151 Bacteroides ovatus ATCC 8483 ALJ47680.1 A7LXT7
3ZMR[A,B] α-D-Xylp-(1-6)-β-D-Glcp-(1-4)[α-D-Xylp-(1-6)]-β-D-Glcp-(1-4)[α-D-Xylp-(1-6)]-β-D-Glcp-(1-1)-<non_carb>
α-D-Xylp-(1-6)-β-D-Glcp-(1-4)[α-D-Xylp-(1-6)]-β-D-Glcp-(1-4)[α-D-Xylp-(1-6)]-β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-GLU
 1.43
4
 endo-cellulase (AC2aCel5A) (Cel5A) 3.2.1.4 Bacteroidetes bacterium AC2a AIJ19564.1 A0A076MPD7
4YHE[A,B] 1.85
4
 bpr_I1710 (Cel5C)   Butyrivibrio proteoclasticus B316 ADL34447.1  
4NF7[A] 2.11
4
 β-1,3-1,4-glucanase / lichenase (F32EG5) 3.2.1.6 Caldicellulosiruptor sp. F32 AGM71677.1 R9RX81
4X0V[A,B,C,D,E,F,G,H] 2.80
5H4R[A] β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp
 1.70
4
 endo-xyloglucanase (CjGH5D; CJA_3010) (Cel5D) 3.2.1.151 Cellvibrio japonicus Ueda107 ACE84905.1 B3PD52
5OYC[A,B] 1.60
5OYD[A,B] α-D-Xylp-(1-6)-β-D-Glcp-(1-4)[α-D-Xylp-(1-6)]-β-D-Glcp-(1-4)[α-D-Xylp-(1-6)]-β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-<non_carb>
β-D-Glcp-(1-4)[α-D-Xylp-(1-6)]-β-D-Glcp-(1-4)[α-D-Xylp-(1-6)]-β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-<non_carb>
 2.10
5OYE[A,B] α-D-Xylp-(1-6)[β-D-Glcp-(1-4)]-β-D-Glcp-(1-4)[α-D-Xylp-(1-6)]-β-D-Glcp-(1-4)-β-D-Glcp
α-D-Xylp-(1-6)[β-D-Glcp-(1-4)]-β-D-Glcp-(1-4)[β-D-Galp-(1-2)-α-D-Xylp-(1-6)]-β-D-Glcp-(1-4)-β-D-Glcp
 1.90
6HA9[A,B] α-D-Xylp-(1-6)-β-D-Glcp-(1-4)[α-D-Xylp-(1-6)]-β-D-Glcp-(1-4)[α-D-Xylp-(1-6)]-β-D-Glcp-(1-4)-β-D-Glcp2F-(1-1)-<non_carb>
α-D-Xylp-(1-6)[β-D-Glcp-(1-4)]-β-D-Glcp-(1-4)[α-D-Xylp-(1-6)]-β-D-Glcp-(1-4)-β-D-Glcp2F-(1-1)-<non_carb>
 2.00
6HAA[A,B] α-D-Xylp-(1-6)-β-D-Glcp-(1-4)[α-D-Xylp-(1-6)]-β-D-Glcp-(1-4)[α-D-Xylp-(1-6)]-β-D-Glcp-(1-4)-α-D-Glcp2F-(1-4)-GLU
α-D-Xylp-(1-6)[β-D-Glcp-(1-4)]-β-D-Glcp-(1-4)[α-D-Xylp-(1-6)]-β-D-Glcp-(1-4)-α-D-Glcp2F-(1-4)-GLU
 1.70
8OZ1[A] <carb_like_YLL>
 1.30
4
 glucomannanase (CAC0826) 3.2.1.151
3.2.1.-		 Clostridium acetobutylicum ATCC 824 AAK78802.1
NP_347462.1		 Q97KU0
6PZ7[A] 1.25
4
 endo-β-1,4-glucanase / xylanase D (EngD) 3.2.1.4
3.2.1.8		 Clostridium cellulovorans ATCC 35269 AAA23233.1 P28623
3ICG[A,B,C,D] 2.10
3NDY[A,B,C,D] 2.10
3NDZ[A,B,C,D] β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp
 2.08
4
 endo-β-1,4-glucanase E / dual cellulase-mannanase (EngE) 3.2.1.4
3.2.1.-		 Clostridium cellulovorans ATCC 35269 AAD39739.1 D9SV64
Q9XD99
6UI3[A] 1.30
4
 endo-β-1,4-glucanase (CelA) 3.2.1.4 Clostridium longisporum AAC37035.1 P54937
6Q1I[A,B] 1.35
4
 β-glucanase (CelB; BhCel5B; BH0603) (Cel5B) 3.2.1.4 Halalkalibacterium halodurans C-125 BAB04322.1
NP_241469.1		 Q9KF82
4V2X[A] 1.64
4
 xyloglucanase (XG5; PpXG5) 3.2.1.151 Paenibacillus pabuli AAR65335.1 H9KVH3
2JEP[A,B] 1.4
2JEQ[A] α-D-Xylp-(1-6)[β-D-Glcp-(1-4)]-β-D-Glcp-(1-4)[β-D-Galp-(1-2)-α-D-Xylp-(1-6)]-β-D-Glcp-(1-4)-β-D-Glcp
 1.94
4
 endo-xyloglucanase (Bacsa_0886) 3.2.1.151 Phocaeicola salanitronis DSM 18170 ADY35478.1 F0R2T3
6WQY[A] 1.05
4
 endo-β-1,4-glucanase A (CelA; Cca; RceCel5A; Ccel_1099) (Cel5A) 3.2.1.4 Ruminiclostridium cellulolyticum H10 AAA23221.1
AAA51444.1
ACL75458.1		 P17901
1EDG[A] 1.60
4
 glucomannanase (RUM_05520) 3.2.1.- Ruminococcus champanellensis 18P13 = JCM 17042 CBL16772.1 D4LAX7
6WQP[A,B] 1.60
6WQV[A,B,C,D] β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-α-D-Glcp
 1.45
4
 glucomannanase (RfGH5-1/2) 3.2.1.- Ruminococcus flavefaciens WP_009984467.1 A0A8I3B056
6XSU[A,B] 1.41
4
 mixed-linkage β-glucanase/xyloglucanase (Orf4; CMCase; PbrCel5A; PBR_0368) (Cel5A) 3.2.1.73
3.2.1.151		 Segatella bryantii B14 AAC97596.1
AAA22909.1		 D8DY19
O06842
Q44878
3L55[A,B] 1.60
3VDH[A,B] 1.62
5D9M[A,B] α-D-Xylp-(1-6)-β-D-Glcp-(1-4)[α-D-Xylp-(1-6)]-β-D-Glcp-(1-4)[α-D-Xylp-(1-6)]-β-D-Glcp-(1-4)-α-D-Glcp
α-D-Xylp-(1-6)-β-D-Glcp-(1-4)[α-D-Xylp-(1-6)]-β-D-Glcp-(1-4)[α-D-Xylp-(1-6)]-β-D-Glcp-(1-4)-β-D-Glcp-(1-4)[α-D-Xylp-(1-6)]-β-D-Glcp-(1-4)[α-D-Xylp-(1-6)]-β-D-Glcp-(1-4)[α-D-Xylp-(1-6)]-β-D-Glcp-(1-4)-α-D-Glcp
 1.90
5D9N[A,B] α-D-Xylp-(1-6)-β-D-Glcp-(1-4)[α-D-Xylp-(1-6)]-β-D-Glcp-(1-4)[α-D-Xylp-(1-6)]-β-D-Glcp-(1-4)-β-D-Glcp
 1.86
5D9O[A,B] β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp
 1.55
5D9P[A,B] α-D-Xylp-(1-6)-β-D-Glcp-(1-4)[α-D-Xylp-(1-6)]-β-D-Glcp-(1-4)[α-D-Xylp-(1-6)]-β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-<non_carb>
α-D-Xylp-(1-6)-β-D-Glcp-(1-4)[α-D-Xylp-(1-6)]-β-D-Glcp-(1-4)[α-D-Xylp-(1-6)]-β-D-Glcp-(1-4)-β-D-Glcp1N-(1-1)-<non_carb>
 1.80
6D2W[A,B] 2.10
4
 xyloglucan-specific endo-β-1,4-glucanase (XEG5A) (Xeg5A) 3.2.1.151 uncultured bacterium ACZ54907.1 D2K7Z0
4W84[A,B] 1.79
4W85[A,B] β-D-Glcp
 1.92
4W86[A,B] β-D-Glcp
 2.64
4W87[A,B] α-D-Xylp-(1-6)-β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp
 2.15
4W88[A,B] β-D-Galp-(1-2)-α-D-Xylp-(1-6)[α-D-Xylp-(1-6)-β-D-Glcp-(1-4)]-β-D-Glcp-(1-4)-β-D-Glcp
β-D-Galp-(1-2)-α-D-Xylp-(1-6)[β-D-Glcp-(1-4)]-β-D-Glcp
 1.58
4W89[A,B] β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-α-D-Glcp
 2.40
4
 exo-xyloglucanase (XEG5B) (Xeg5B) 3.2.1.150 uncultured bacterium ADB44000.1 D2XML9
4W8A[A] 1.72
4W8B[A] α-D-Xylp-(1-6)-β-D-Glcp-(1-4)[α-D-Xylp-(1-6)]-β-D-Glcp-(1-4)-β-D-Glcp
α-D-Xylp-(1-6)-β-D-Glcp-(1-4)[α-D-Xylp-(1-6)]-β-D-Glcp-(1-4)[β-D-Galp-(1-2)-α-D-Xylp-(1-6)]-β-D-Glcp-(1-4)-α-D-Glcp
 1.15
4
 endo-β-1,4-glucanase (YBcontig310-00424-17) 3.2.1.4 uncultured bacterium ADU86909.1 H2BCH8
6XSO[A,B] 1.50
4
 endo-β-1,3-1,4-glucanase (EngA; ZgEngA; zobellia_208) 3.2.1.6 Zobellia galactanivorans DsijT CAZ94281.1 G0L8Z3
6GL0[A,B,C] β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-α-D-Glcp
 2.20
6GL2[A] 1.96
4
Eukaryota
Protein Name EC# OrganismGenBankUniprot
PDB/3D Carbohydrate Ligands Resolution (Å)
Subf
 BCR36DRAFT_398243   Piromyces finnis ORX48147.1  
8GHX[A,B] 2.46
8GHY[A,B] β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp
 1.80
4
 endoglucanase A (CelA;EglA) (partial) 3.2.1.4 Piromyces rhizinflatus 2301 AAF00074.2 Q9URH5
3AYR[A] 2.00
3AYS[A] β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp
 2.20
4
 endo-xyloglucanase (Type 1-7.1;partial) 3.2.1.151 uncultured bovine rumen ciliate AEQ16450.1 G5DDB8
6XRK[A,B] 1.42
4
unclassified
Protein Name EC# OrganismGenBankUniprot
PDB/3D Carbohydrate Ligands Resolution (Å)
Subf
 SARM_0063 / 2527869_58630 / TW-33 3.2.1.4 uncultured organism ADX05734.1 A0A076MPD7
E9NSN9
4YHG[A,B] β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp
 2.40
4
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