Glycoside Hydrolase Family 5 / Subf 4

Activities in Familyendo-β-1,4-glucanase / cellulase (EC 3.2.1.4); endo-β-1,4-xylanase (EC 3.2.1.8); β-glucosidase (EC 3.2.1.21); β-mannosidase (EC 3.2.1.25); β-glucosylceramidase (EC 3.2.1.45); glucan β-1,3-glucosidase (EC 3.2.1.58); licheninase (EC 3.2.1.73); exo-β-1,4-glucanase / cellodextrinase (EC 3.2.1.74); glucan endo-1,6-β-glucosidase (EC 3.2.1.75); mannan endo-β-1,4-mannosidase (EC 3.2.1.78); cellulose β-1,4-cellobiosidase (EC 3.2.1.91); steryl β-glucosidase (EC 3.2.1.104); endoglycoceramidase (EC 3.2.1.123); chitosanase (EC 3.2.1.132); β-primeverosidase (EC 3.2.1.149); xyloglucan-specific endo-β-1,4-glucanase (EC 3.2.1.151); endo-β-1,6-galactanase (EC 3.2.1.164); hesperidin 6-O-α-L-rhamnosyl-β-glucosidase (EC 3.2.1.168); β-1,3-mannanase (EC 3.2.1.-); arabinoxylan-specific endo-β-1,4-xylanase (EC 3.2.1.-); mannan transglycosylase (EC 2.4.1.-)
Activities in Sub Family
Mechanism Retaining
ClanGH-A
3D Structure Status( β / α ) 8
Catalytic Nucleophile/BaseGlu (experimental)
Catalytic Proton DonorGlu (experimental)
NoteOnce known as cellulase family A; many members have been assigned to subfamilies as described by Aspeborg et al. (2012) BMC Evol Biol. 12(1):186 (PMID: 22992189).
External resourcesCAZypedia; HOMSTRAD; PROSITE;
Commercial Enzyme Provider(s)MEGAZYME; NZYTech; PROZOMIX;
Statistics GenBank accession (596); Uniprot accession (185); PDB accession (34); 3D entries (16); cryst (0)
Bacteria
Protein Name EC#OrganismGenBank UniprotPDB/3DSubf
 β-glucanase (CelB;BhCel5B;BH0603) (Cel5B) 3.2.1.4 Bacillus halodurans C-125 BAB04322.1
NP_241469.1
Q9KF82 4V2X[A] 4
 endoglucanase (BlCel5B;BLi01882;BL01230) (Cel5B) 3.2.1.4 Bacillus licheniformis DSM 13 = ATCC 14580 AAU40777.1
AAU23417.1
Q65JI7 4YZP[A]
4YZT[A]
4
 cellulase (CelB) 3.2.1.4 Bacillus sp. BG-CS10 ADD62401.1 D4P8C6 5E09[A]
5E0C[A]
4
 endo-xyloglucanase (CelA;BoGH5A;BACOVA_02653;Bovatus_03072) 3.2.1.151 Bacteroides ovatus ATCC 8483 ALJ47680.1   3ZMR[A,B] 4
 endo-cellulase (AC2aCel5A) (Cel5A) 3.2.1.4 Bacteroidetes bacterium AC2a AIJ19564.1   4YHE[A,B] 4
 endo-β-1,4-glucanase 1 (End1;EglI;EGI) 3.2.1.4
3.2.1.73
Butyrivibrio fibrisolvens H17C CAA35574.1 P20847   4
 endoglucanase / xylanase (CGSCsYakCAS_09989) (Cel5A) 3.2.1.- Cellulosilyticum ruminicola CGMCC 1.5065 ACZ98591.1 D2KFI9   4
 endo-/exo-type cellulase (CelEdx16) 3.2.1.4 Clostridiaceae bacterium AN-C16-KBRB ADK66823.1 E0A3Q5   4
 endo-β-1,4-glucanase A (CelA;Cca;CelCcaCcel_1099) (Cel5A) 3.2.1.4 [Clostridium] cellulolyticum H10 AAA23221.1
AAA51444.1
ACL75458.1
P17901 1EDG[A] 4
 endo-β-1,4-glucanase / xylanase B (EngB;Clocel_1150) 3.2.1.4
3.2.1.73
3.2.1.8
Clostridium cellulovorans 743B AAA23231.1
ADL50906.1
C5RHC3
P28621
  4
 endo-β-1,4-glucanase / xylanase D (EngD) 3.2.1.4
3.2.1.73
3.2.1.8
Clostridium cellulovorans ATCC 35269 AAA23233.1 P28623 3ICG[A,B,C,D]
3NDY[A,B,C,D]
3NDZ[A,B,C,D]
4
 endo-β-1,4-glucanase E (EngE) 3.2.1.4 Clostridium cellulovorans ATCC 35269 AAD39739.1 Q9XD99   4
 endo-β-1,4-glucanase (CelA) 3.2.1.4 Clostridium longisporum AAC37035.1 P54937   4
 endoglucanase (EcCel5A) (Cel5A) 3.2.1.4 Eubacterium cellulosolvens 5 BAE46390.1 Q3LHN3   4
 endoglucanase (EcCel5A) (Cel5A) 3.2.1.4 Eubacterium cellulosolvens 5 BAE46390.1 Q3LHN3   4
 Fisuc_0786 / FSU_1228 3.2.1.4 Fibrobacter succinogenes subsp. succinogenes S85 ACX74396.1
ADL25822.1
C9RNF0   4
 Fisuc_3081 / FSU_0347 3.2.1.4 Fibrobacter succinogenes subsp. succinogenes S85 ACX76661.1
ADL25927.1
C9RQB4   4
 endo-β-1,4-glucanase G (CelG;Fisuc_0897;FSU_1346) 3.2.1.4 Fibrobacter succinogenes subsp. succinogenes S85 AAB38548.1
ACX74504.1
ADL25356.1
Q47916   4
 endo-β-1,4-glucanase (CpCel5C;Cphy_2058) (Cel5C) 3.2.1.4 Lachnoclostridium phytofermentans ISDg ABX42426.1 A9KIH4   4
 endo-β-1,4-glucanase A (CelA) 3.2.1.4 Paenibacillus barcinonensis BP-23 CAA73113.1 O08342   4
 endo-β-1,4-glucanase B (CelB;EG-B) 3.2.1.4 Paenibacillus lautus PL236 AAA22408.1
CAA01934.1
P23550   4
 xyloglucanase (XG5;PpXG5) 3.2.1.151 Paenibacillus pabuli AAR65335.1 H9KVH3 2JEP[A,B]
2JEQ[A]
4
 cellulase 5B (Cel5B) 3.2.1.4 Paenibacillus polymyxa GS01 ABV08876.1 A9Q742   4
 Sequence 4 from patent US 6630340 3.2.1.151 Paenibacillus sp. AAR65336.1     4
 xyloglucanase (XEG5) (Xeg5) 3.2.1.151 Paenibacillus sp. KM21 BAE44526.1 Q3MUH8   4
 endo-β-1,4-glucanase (Cel5A) 3.2.1.4 Paenibacillus xylanilyticus KJ-03 AFC68970.1     4
 mixed-linkage β-glucanase/xyloglucanase (Orf4;CMCase;PBR_0368) (Cel5A) 3.2.1.151
3.2.1.73
Prevotella bryantii B14 AAC97596.1
AAA22909.1
O06842
Q44878
3L55[A,B]
3VDH[A,B]
5D9M[A,B]
5D9N[A,B]
5D9O[A,B]
5D9P[A,B]
4
 xylanase / endoglucanase (PRU_2231) 3.2.1.4
3.2.1.8
Prevotella ruminicola 23 AAC36862.1
ADE83418.1
D5EV54
Q45397
  4
 endoglucanase (CelA;BrEND) 3.2.1.4 Prevotella ruminicola AR20 1814455A     4
 endo-β-1,4-glucanase E (CelE;EgE) (Cel5C) 3.1.1.72
3.2.1.4
Ruminiclostridium thermocellum AAA23224.1 P10477 4IM4[A,B,C,D,E,F] 4
 acetylxylan esterase / 6-O-glycoside deacetylase (CelE;CtCE2;Cthe_0797) (Cel5C) 3.1.1.72
3.1.1.-
Ruminiclostridium thermocellum ATCC 27405 ABN52032.1 A3DDK3   4
 Rumal_1082 (Cel5D) 3.2.1.4 Ruminococcus albus 7 = DSM 20455 ADU21608.1 E0L8I8   4
 endo-1,4-glucanase I (EGI) 3.2.1.4 Ruminococcus albus F-40 AAA26469.1 P16216   4
 endo-1,4-glucanase V (EgV) 3.2.1.4 Ruminococcus albus F-40 BAA92146.1 Q9LCC5   4
 β-1,4-endoglucanase VII (EgVII) (Cel5D) 3.2.1.4 Ruminococcus albus F-40 BAA92430.1 Q9LCC4   4
 endo-β-1,4-glucanase A (CelA;EgA) 3.2.1.4 Ruminococcus albus SY3 AAA26467.1
CAA38692.1
P23660
Q59733
  4
 endo-β-1,4-glucanase B (CelB;EgB) 3.2.1.4 Ruminococcus albus SY3 CAA38693.1 P23661   4
 endo-β-1,4-glucanase (GH5A;RUM_02800) (Cel5A) 3.2.1.4 Ruminococcus champanellensis 18P13 = JCM 17042 CBL16523.1 D4LA78   4
 endo-β-1,4-glucanase A (EndA) 3.2.1.4 Ruminococcus flavefaciens 17 CAB05881.1
AAB19708.1
O05143
Q53302
  4
 endo-β-1,4-glucanase 5D (Sde_2636) (Cel5D) 3.2.1.4 Saccharophagus degradans 2-40 ABD81896.1 Q21HD3   4
 cellulase (EngV) (fragment) 3.2.1.4 Salinivibrio sp. NTU-05 ACJ71329.1 B8XGR7   4
 cellulase (Cel;BagCel) 3.2.1.4 Salipaludibacillus agaradhaerens DSM 8721 CAD61244.1 Q6A3Q5   4
 xyloglucan-specific endo-β-1,4-glucanase (XEG5A) (Xeg5A) 3.2.1.151 uncultured bacterium ACZ54907.1 D2K7Z0 4W84[A,B]
4W85[A,B]
4W86[A,B]
4W87[A,B]
4W88[A,B]
4W89[A,B]
4
 bifunctional mannanase-xyloglucanase (MNXEG) 3.2.1.73
3.2.1.78
uncultured bacterium ADA62505.1 D2KKK4   4
 exo-xyloglucanase (XEG5B) (Xeg5B) 3.2.1.- uncultured bacterium ADB44000.1 D2XML9 4W8A[A]
4W8B[A]
4
 EXO02C10-ORF13 (En5) 3.2.1.- uncultured bacterium AEV59735.1
AGO64722.1
AGO64723.1
AGO64724.1
AGO64725.1
AGO64726.1
AGO64727.1
AGO64728.1
AGO64729.1
AGO64730.1
AGO64731.1
AGO64732.1
    4
 endo-β-1,4-glucanase (En3;CMC6A4-ORF12) 3.2.1.4 uncultured bacterium AEV59725.1     4
 cellulase (Exo2b;EXO02C10-ORF14) 3.2.1.4 uncultured bacterium AEV59736.1     4
 cellulase (YBcontig404-00027-37) 3.2.1.4 uncultured bacterium ADU86901.1     4
 cellulase (YBcontig310-00038-19) 3.2.1.4 uncultured bacterium ADU86902.1     4
 endoglycosidase (GH6248) (Cel5A) 3.2.1.4
3.2.1.73
3.2.1.78
3.2.1.8
uncultured bacterium ABB46200.1 Q283N5   4
 endoglucanase 3.2.1.4 uncultured bacterium Contig33 AHF24998.1     4
 endoglucanase (GH5_h) 3.2.1.- uncultured Bacteroidetes bacterium AIT97141.1     4
 endoglucanase (GH5_g) 3.2.1.- uncultured Bacteroidetes bacterium AIT97140.1     4
Eukaryota
Protein Name EC#OrganismGenBank UniprotPDB/3DSubf
 endo-β-1,4-glucanase (Epi3) (probable fragment) 3.2.1.4 Epidinium caudatum BAA76394.1 Q9XXV3   4
 CMCase (Cmc-06) (Cel5A) 3.2.1.4 Epidinium ecaudatum CAH69214.1
CAL91975.1
Q5F324   4
 endo-β-1,4-glucanase A (CelA) 3.2.1.4 Neocallimastix frontalis AAC63094.1 Q01409   4
 endo-β-1,4-glucanase B (CelB) 3.2.1.4 Neocallimastix patriciarum AAE59927.1
CAA83238.1
Q12647   4
 endo-β-1,4-glucanase D (CelD;W5-CelD;W5C-P) (Cel5D) 3.2.1.4
3.2.1.4
3.2.1.73
3.2.1.73
Neocallimastix patriciarum W5 AAC06321.1
AEX92707.1
O59943   4
 endo-β-1,4-glucanase D (CelD;W5-CelD;W5C-P) (Cel5D) 3.2.1.4
3.2.1.4
3.2.1.73
3.2.1.73
Neocallimastix patriciarum W5 AAC06321.1 O59943   4
 endo-β-1,4-glucanase D (CelD;W5-CelD;W5C-P) (Cel5D) 3.2.1.4
3.2.1.4
3.2.1.73
3.2.1.73
Neocallimastix patriciarum W5 AAC06321.1 O59943   4
 endo-β-1,4-glucanase (CelB2) (fragment) 3.2.1.4 Orpinomyces joyonii SG4 AAB69348.1 O13334   4
 endo-β-1,4-glucanase (CelB29) 3.2.1.4 Orpinomyces joyonii SG4 AAB69347.1 O13333   4
 endo-β-1,4-glucanase B (CelB) 3.2.1.4 Orpinomyces sp. PC-2 AAD04193.1
AAE59925.1
P78719   4
 endo-β-1,4-glucanase E (CelE) 3.2.1.4 Orpinomyces sp. PC-2 AAC05164.1 Q9HFE0   4
 endoglucanase 5C (PdCel5C) (Cel5C) 3.2.1.4 Penicillium decumbens 114-2 AFG25592.1     4
 endoglucanase 5A (Cel5A) (Cel5A) 3.2.1.4 Piromyces equi CAB92326.1 Q9P867   4
 endoglucanase 5A (Cel5A) (Cel5A) 3.2.1.4 Piromyces equi CAB92326.1 Q9P867   4
 endoglucanase 5A (Cel5A) (Cel5A) 3.2.1.4 Piromyces equi CAB92326.1 Q9P867   4
 endoglucanase 5A (Cel5A) (Cel5A) 3.2.1.4 Piromyces equi CAB92326.1 Q9P867   4
 endoglucanase A (CelA;EglA) (partial) 3.2.1.4 Piromyces rhizinflatus 2301 AAF00074.2   3AYR[A]
3AYS[A]
4
 endoglucanase (Egl-1) (Cel5B) 3.2.1.4 Piromyces rhizinflatus 2301 AAD43818.1 Q9Y870   4
unclassified
Protein Name EC#OrganismGenBank UniprotPDB/3DSubf
 cellulase (BT-01) (partial) 3.2.1.4 uncultured microorganism ADR64663.1 E5F2K8   4
 β-glucanase (RuCelA;C29-2) 3.2.1.- uncultured microorganism ACA61132.1 B1PLH5   4
 β-glucanase (C35-2) 3.2.1.- uncultured microorganism ACA61135.1 B1PLH8   4
 β-glucanase (C67-1) 3.2.1.- uncultured microorganism ACA61137.1 B1PLI0   4
 β-glucanase (C5614-1) 3.2.1.- uncultured microorganism ACA61140.1 B1PLI3   4
 β-glucanase (DC2-4) 3.2.1.- uncultured microorganism ACA61145.1 B1PLI8   4
 β-glucanase (DC3-1) 3.2.1.4 uncultured microorganism ACA61149.1 B1PLJ2   4
 β-glucanase (DC23-3) 3.2.1.- uncultured microorganism ACA61160.1 B1PLK3   4
 β-glucanase 3.2.1.- uncultured organism ADD61809.1 D9ZEQ5   4
 β-glucanase 3.2.1.4 uncultured organism ADD61911.1 D9ZF07   4
 SARM_0071 / 181694_69520 / TX-1 3.2.1.4 uncultured organism ADX05742.1     4
 SARM_0034 / 694713_55880 / TW-39 3.2.1.4 uncultured organism ADX05705.1     4
 SARM_0032 / 558318_19410 / MH-20 3.2.1.4 uncultured organism ADX05703.1     4
 SARM_0017 / 3953955_161040 / TW-49 3.2.1.4 uncultured organism ADX05688.1     4
 SARM_0084 / 0_04358 / TW-56 (fragment; probable frameshift) 3.2.1.4 uncultured organism ADX05760.1     4
 SARM_0063 / 2527869_58630 / TW-33 3.2.1.4 uncultured organism ADX05734.1   4YHG[A,B] 4
 SARM_0058 / 2395619_81340 / TW-44 3.2.1.4 uncultured organism ADX05729.1     4
 β-glucanase (RR.01;RR.01-2) 3.2.1.- unidentified microorganism CAJ19135.1 Q2YI68   4
 glucanase (RR.02-2;BglC) 3.2.1.- unidentified microorganism CAJ19139.1 Q2YI64   4
 β-glucanase (RR.06;RR.06-1;BglC) 3.2.1.- unidentified microorganism CAJ19140.1 Q2YI63   4
 glucanase (RR.10;RR.10-1) 3.2.1.- unidentified microorganism CAJ19146.1 Q2YI57   4
 glucanase (RR.14;RR.14-2) (Cel5A) 3.2.1.4 unidentified microorganism CAJ19151.1 Q2YI52   4
 endo-β-1,4-glucanase (UmCel28;CC1) 3.2.1.4 unidentified microorganism ABX76045.1 A9UGW1   4
 endoglucanase (UmCelE2;EC2) 3.2.1.4 unidentified microorganism ABX76046.1 A9UGW2   4
 endoglucanase (UmCelS15;SC1) (fragment) 3.2.1.4 unidentified microorganism ABX76048.1 A9UGW4   4
 endoglucanase (UmCelC23;CC2) 3.2.1.4 unidentified microorganism ABX76050.1 A9UGW6   4
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