Glycoside Hydrolase Family 48

Known Activitiesreducing end-acting cellobiohydrolase (EC 3.2.1.176); endo-β-1,4-glucanase (EC 3.2.1.4); chitinase (EC 3.2.1.14)
Mechanism Inverting
ClanGH-M
3D Structure Status( α / α ) 6
Catalytic Nucleophile/BaseNot known
Catalytic Proton DonorGlu
Noteformerly known as cellulase family L. Some cellobiohydrolases of this family have been reported to act from the reducing ends of cellulose (EC 3.2.1.-), while others have been reported to operate from the non-reducing ends to liberate cellobiose or cellotriose or cellotetraose (EC 3.2.1.-). This family also contains endo-processive cellulases (EC 3.2.1.-), whose activity is hard to distinguish from that of cellobiohydrolases.
External resourcesCAZypedia; HOMSTRAD; InterPro; PFAM; PRINTS;
Commercial Enzyme Provider(s)NZYTech;
Statistics GenBank accession (175); Uniprot accession (113); PDB accession (11); 3D entries (2); cryst (0)
Bacteria
Protein Name EC#OrganismGenBankUniprotPDB/3D
 cellulase (CelA;Athe_1867) 3.2.1.4 Anaerocellum thermophilum DSM 6725 ACM60955.1
CAB06786.1
B9MKU7
P96311
 
 cellobiohydrolase B (Cbp120;CbhB;CenE) (Cel48A) 3.2.1.176 Cellulomonas fimi ATCC 484 AAA50257.1
AAB00822.1
ACH05303.1
P50899  
 processive endo-cellulase F (CelF;Ccel_0729) (Cel48F) 3.2.1.176 Clostridium cellulolyticum H10 ATCC 35319 AAA73866.1
AAB41452.1
ACL75108.1
P37698 1F9D[A]
1F9O[A]
1FAE[A]
1FBO[A]
1FBW[A]
1FCE[A]
1G9G[A]
1G9J[A]
2QNO[A]
 exoglucanase S (ExgS;P70) 3.2.1.176 Clostridium cellulovorans AAC38571.2
AAC38571.3
O65986  
 cellobiohydrolase (CpCel48;Cphy_3368) (Cel48) 3.2.1.176 Clostridium phytofermentans ISDg ABX43721.1 A9KT91  
 cellodextrinohydrolase (avicelase II) (CelY) 3.2.1.176 Clostridium stercorarium CAA93280.1 P50900  
 endo-β-1,4-glucanase Y (CelY;Cthe_0071) (Cel48B=Cel48Y) 3.2.1.4 Clostridium thermocellum ATCC 27405 ABN51312.1 A3DBI3  
 exo-cellulase S (CelS;Cthe_2089) (Cel48A=Cel48S) 3.2.1.176 Clostridium thermocellum ATCC 27405 ABN53296.1 A3DH67  
 cellobiohydrolase (CelS) (Cel48A) 3.2.1.176 Clostridium thermocellum F7 AAA23226.1 P0C2S5
P38686
1L1Y[A,B,C,D,E,F]
1L2A[A,B,C,D,E,F]
 cellobiohydrolase (Cel48) 3.2.1.176 Myxobacter sp. AL-1 ABD64772.1 Q19VP0  
 cellobiohydrolase (Cel48C) 3.2.1.176 Paenibacillus barcinonensis BP-23 CAD32945.1 Q8KKF7  
 cellulase (Cel48A) 3.2.1.4 Ruminococcus albus 8 AAR01217.1 Q6TF32  
 β-1,4-exocellulase (E6;CelF;Tfu_1959) (Cel48A) 3.2.1.176 Thermobifida fusca YX AAD39947.1
AAZ55992.1
ACH05304.1
Q47NH7
Q9XCD4
 
Eukaryota
Protein Name EC#OrganismGenBankUniprotPDB/3D
 chitinase (active phase-associated protein I) (Apap1) 3.2.1.14 Gastrophysa atrocyanea BAE94320.1 Q1JUL1  

Footnotes

Experimental characterization exclusively covers enzyme activities at present. If you are aware of any missing experimental characterization that should be mentioned here, we encourage you to send your comment and support of the experimental evidence, including an electronic copy or appropriate link to the support material (i.e., article(s), thesis, database, etc), to cazy@afmb.univ-mrs.fr.





Last update: 2012-01-31 © Copyright 1998-2012
AFMB - CNRS - Universités Aix-Marseille I & II