Glycoside Hydrolase Family 13

Activities in Familyα-amylase (EC 3.2.1.1); pullulanase (EC 3.2.1.41); cyclomaltodextrin glucanotransferase (EC 2.4.1.19); cyclomaltodextrinase (EC 3.2.1.54); trehalose-6-phosphate hydrolase (EC 3.2.1.93); oligo-α-glucosidase (EC 3.2.1.10); maltogenic amylase (EC 3.2.1.133); neopullulanase (EC 3.2.1.135); α-glucosidase (EC 3.2.1.20); maltotetraose-forming α-amylase (EC 3.2.1.60); isoamylase (EC 3.2.1.68); glucodextranase (EC 3.2.1.70); maltohexaose-forming α-amylase (EC 3.2.1.98); maltotriose-forming α-amylase (EC 3.2.1.116); branching enzyme (EC 2.4.1.18); trehalose synthase (EC 5.4.99.16); 4-α-glucanotransferase (EC 2.4.1.25); maltopentaose-forming α-amylase (EC 3.2.1.-) ; amylosucrase (EC 2.4.1.4) ; sucrose phosphorylase (EC 2.4.1.7); malto-oligosyltrehalose trehalohydrolase (EC 3.2.1.141); isomaltulose synthase (EC 5.4.99.11); malto-oligosyltrehalose synthase (EC 5.4.99.15); amylo-α-1,6-glucosidase (EC 3.2.1.33); α-1,4-glucan: phosphate α-maltosyltransferase (EC 2.4.99.16); 6'-P-sucrose phosphorylase (EC 2.4.1.-); amino acid transporter
Mechanism Retaining
ClanGH-H
3D Structure Status( β / α ) 8
Catalytic Nucleophile/BaseAsp (experimental)
Catalytic Proton DonorGlu (experimental)
NoteNew: many members have been assigned to subfamilies as described by Stam et al. (2006) Protein Eng Des Sel. 19, 555-562 (PMID: 17085431)
External resourcesCAZypedia; EBI Protein of the Month; HOMSTRAD; PDB Molecule of the Month; PRINTS;
Commercial Enzyme Provider(s)MEGAZYME; PROZOMIX;
Statistics GenBank accession (54252); Uniprot accession (7858); PDB accession (465); 3D entries (110); cryst (2)
All (49476) Archaea (367) Bacteria (45982) Eukaryota (2997) unclassified (130) Structure (110 - 2 cryst) Characterized (812)
| 1 | 2 |
Eukaryota
Protein Name EC#OrganismGenBankUniprot
PDB/3D Carbohydrate Ligands Resolution (Å)
Subf
 branching-enzyme (Gbe1;HosBE) 2.4.1.18 Homo sapiens AAA58642.1
AAH12098.1
ABE12601.1
BAD92968.1
BAG54265.1
BAG60255.1
BAG62313.1
NP_000149.1
Q04446
Q04446
4BZY[A,B,C] 2.75
5CLT[A,B,C] <non_carb>-(1-4)-α-D-6-deoxy-Glcp4N-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp
2.79
5CLW[A,B,C] α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp
2.80
8
 α-amylase 1 (Amy1;Amy1.1) 3.2.1.1 Hordeum vulgare AAA32929.1
AAA32927.1
P00693
Q40017
1HT6[A] 1.50
1P6W[A] β-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-β-D-Glcp-(1-1)-methyl
β-D-Glcp-α-D-Glcp4S-(1-4)-α-D-Glcp4S-(1-4)-β-D-Glcp4S-(1-1)-methyl
2.00
1RP8[A] α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp
α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-β-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-β-D-Glcp
2.00
1RP9[A] <non_carb>-(1-4)-α-D-6-deoxy-Glcp4N-(1-4)-α-D-Glcp-(1-4)-β-D-Glcp
<non_carb>-(1-4)-α-D-6-deoxy-Glcp4N-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp
2.00
1RPK[A] <non_carb>-(1-4)-α-D-6-deoxy-Glcp4N-(1-4)-α-D-Glcp-(1-4)-β-D-Glcp
<non_carb>-(1-4)-α-D-6-deoxy-Glcp4N-(1-4)-β-D-Glcp
2.00
2QPS[A] 2.20
2QPU[A,B,C] <non_carb>-(1-4)-α-D-6-deoxy-Glcp4N-(1-4)-D-1-deoxy-Glcp
<non_carb>-(1-4)-α-D-6-deoxy-Glcp4N-(1-4)-α-D-Glcp-(1-4)-β-D-Glcp
β-D-Glcp
1.70
3BSG[A] 1.95
3BSH[A] β-D-Glcp
3.00
6
 α-amylase 2 (Amy2) 3.2.1.1 Hordeum vulgare CAA33298.1
AAA32925.1
AAA98790.1
P04063
Q40015
1AMY[A] 2.80
1AVA[A,B] 1.90
1BG9[A] <non_carb>-(1-4)-α-D-6-deoxy-Glcp4N-(1-4)-β-D-Glcp
<non_carb>-(1-4)-β-D-6-deoxy-Glcp4N
2.80
6
 limit dextrinase (HvLD99) 3.2.1.41 Hordeum vulgare AAD04189.1
AAD34347.1
AAD34348.1
AAF98802.1
O48541
Q9FYY0
Q9S7S8
2X4B[A] b-cyclodextrin
2.10
2X4C[A] a-cyclodextrin
2.49
2Y4S[A] b-cyclodextrin
2.10
2Y5E[A] a-cyclodextrin
2.49
4AIO[A] 1.90
4CVW[A,B] 2.67
4J3S[A] α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp
α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp
1.75
4J3T[A] α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-D-1-deoxy-Glcp
1.60
4J3U[A,B] 1.70
4J3V[A] α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp
α-D-Glcp-(1-4)-α-D-Glcp-(1-6)[α-D-Glcp-(1-4)]-α-D-Glcp6SH-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp
1.45
4J3W[A] α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-6)[α-D-Glcp-(1-4)]-α-D-Glcp-(1-4)-α-D-Glcp
1.67
4J3X[A] α-D-Glcp-(1-6)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-6)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp
1.75
13
 α-amylase (AmyA;McAmyA) 3.2.1.1 Malbranchea cinnamomea AFD54462.1  
3VM7[A] α-D-Glcp
2.25
1
 starch-branching enzyme 1 (Sbe1;Rbe1;BEI;OsBEI;Os06g0726400) 2.4.1.18 Oryza sativa Japonica Group BAF20543.1
AAD28284.1
AAP68993.1
AAU99032.1
AAX83629.1
ABA16988.1
ABD15942.1
ABD15943.1
ABD15944.1
ABD15945.1
ABE12599.1
ABL74557.1
ABL74558.1
ACM95329.1
ACY56114.1
ADK61026.1
ADK61027.1
AEB33747.1
AEB33748.1
AEB33749.1
AEB33750.1
AEB33751.1
AEB33752.1
AEB33753.1
AEB33754.1
AHL84201.1
AHL84202.1
AHL84212.1
AII21924.1
BAA01584.1
BAA01616.1
BAA01855.1
BAD61712.1
BAD61713.1
BAD61804.1
BAD61805.1
BAG89369.1
BAS99598.1
BAS99599.1
BAS99600.1
Q01401
Q0D9D0
Q7XZP9
3AMK[A] 1.90
3AML[A] 1.70
3VU2[A,B] α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp
β-D-Glcp
2.23
8
 α-amylase 1A / I-1 (α-Amy7;RAmy1A;Amy1.1;AmyI-1;Os02g0765600) 3.2.1.1 Oryza sativa Japonica Group BAF10134.1
ACA50496.1
BAD17125.1
BAD17313.1
BAG95208.1
BAS81060.1
CAA34516.1
P17654
Q0DXA4
3WN6[A,B,C,D] 2.16
6
 α-amylase (partial) 3.2.1.1 Oryzias latipes    
3VM5[A] 2.85
24
 oligo-α-1,6-glucosidase / isomaltase (Ima1;IMA1;Fsp2;YGR287c) 3.2.1.10 Saccharomyces cerevisiae S288c BAA07818.1
ADK27710.1
CAA97319.1
CAA97321.1
CBF79247.1
NP_011803.1
E1AFY6
P53051
3A47[A] 1.59
3A4A[A] α-D-Glcp
1.60
3AJ7[A] 1.30
3AXH[A] α-D-Glcp-(1-6)-α-D-Glcp
1.80
3AXI[A] α-D-Glcp
1.40
40
 α-amylase (Ppa;PPA) (pancreatic) 3.2.1.1 Sus scrofa AAF02828.1 P00690
1BVN[P] 2.50
1DHK[A] 1.85
1HX0[A] <non_carb>-(1-4)-α-D-6-deoxy-Glcp4N-(1-4)-α-D-Glcp-(1-4)-<non_carb>-(0-4)-α-D-6-deoxy-Glcp4N-(1-4)-α-D-Glcp
α-D-Glcp-(1-4)-α-D-Glcp-(1-1)-<non_carb>
1.38
1JFH[A] α-D-Glcp-(1-4)-α-D-Glcp4S1S
α-D-Glcp4SMe-(1-4)-α-D-Glcp4S-(1-1)-methyl
2.03
1KXQ[A] 1.60
1KXT[A] 2.00
1KXV[A] 1.60
1OSE[A] <non_carb>-(1-4)-α-D-6-deoxy-Glcp4N-(1-4)-α-D-Glcp-(1-4)-<non_carb>-(0-4)-α-D-6-deoxy-Glcp4N-(1-4)-β-D-Glcp
β-D-Glcp
2.30
1PIF[A] 2.30
1PIG[A] α-D-6-deoxy-Glcp4N-(1-4)-α-D-Glcp-(1-4)-<non_carb>-(0-4)-α-D-6-deoxy-Glcp4N-(1-4)-α-D-Glcp-(1-4)-β-D-Glcp
α-D-Glcp-(1-4)-α-D-Glcp
β-D-Glcp
β-D-Glcp-(1-4)-α-D-Glcp
2.20
1PPI[A] α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-<non_carb>-(0-4)-α-D-6-deoxy-Glcp4N-(1-4)-β-D-Glcp
2.20
1UA3[A] α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp
α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-β-D-Glcp
α-D-Glcp-(1-4)-β-D-Glcp
β-D-Glcp
2.01
1VAH[A] 2.40
1WO2[A] α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-β-D-Glcp
α-D-Glcp-(1-4)-β-D-Glcp
2.01
3L2L[A] α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp
α-D-Glcp-(1-4)-α-D-Glcp-(1-4)[α-D-Glcp-(1-6)]-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp
β-D-Glcp
2.11
3L2M[A] 1.97
4X0N[A] 2.60
24
 α-amylase 3.2.1.1 Tenebrio molitor   P56634
1CLV[A] 2.00
1JAE[A] 1.65
1TMQ[A] 2.50
1VIW[A] 3.00
15
Top


Last update: 2017-10-09 © Copyright 1998-2017
AFMB - CNRS - Université d'Aix-Marseille