Glycoside Hydrolase Family 13 / Subf 5

Activities in Familyα-amylase (EC 3.2.1.1); pullulanase (EC 3.2.1.41); cyclomaltodextrin glucanotransferase (EC 2.4.1.19); cyclomaltodextrinase (EC 3.2.1.54); trehalose-6-phosphate hydrolase (EC 3.2.1.93); oligo-α-glucosidase (EC 3.2.1.10); maltogenic amylase (EC 3.2.1.133); neopullulanase (EC 3.2.1.135); α-glucosidase (EC 3.2.1.20); maltotetraose-forming α-amylase (EC 3.2.1.60); isoamylase (EC 3.2.1.68); glucodextranase (EC 3.2.1.70); maltohexaose-forming α-amylase (EC 3.2.1.98); maltotriose-forming α-amylase (EC 3.2.1.116); branching enzyme (EC 2.4.1.18); trehalose synthase (EC 5.4.99.16); 4-α-glucanotransferase (EC 2.4.1.25); maltopentaose-forming α-amylase (EC 3.2.1.-) ; amylosucrase (EC 2.4.1.4) ; sucrose phosphorylase (EC 2.4.1.7); malto-oligosyltrehalose trehalohydrolase (EC 3.2.1.141); isomaltulose synthase (EC 5.4.99.11); malto-oligosyltrehalose synthase (EC 5.4.99.15); amylo-α-1,6-glucosidase (EC 3.2.1.33); α-1,4-glucan: phosphate α-maltosyltransferase (EC 2.4.99.16); 6'-P-sucrose phosphorylase (EC 2.4.1.-); amino acid transporter
Activities in Sub Family
Mechanism Retaining
ClanGH-H
3D Structure Status( β / α ) 8
Catalytic Nucleophile/BaseAsp (experimental)
Catalytic Proton DonorGlu (experimental)
NoteNew: many members have been assigned to subfamilies as described by Stam et al. (2006) Protein Eng Des Sel. 19, 555-562 (PMID: 17085431)
External resourcesCAZypedia; EBI Protein of the Month; HOMSTRAD; PDB Molecule of the Month; PRINTS;
Commercial Enzyme Provider(s)MEGAZYME; PROZOMIX;
Statistics GenBank accession (2341); Uniprot accession (238); PDB accession (24); 3D entries (8); cryst (0)
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | ... | 21 |
Bacteria
Protein Name EC#Organism GenBankUniprotPDB/3DSubf
 α-amylase, partial   Bacillus megaterium AGH01 AMX23350.1     5
 BMD_5229 (AmyL)   Bacillus megaterium DSM 319 ADF42028.1 D5DME4   5
 BUW91_25915   Bacillus megaterium JX285 AQU76510.1     5
 maltopentaose-forming α-amylase 3.2.1.- Bacillus megaterium KSM B-404 AAK00598.1 Q9AQ54   5
 BG04_2237   Bacillus megaterium NBRC 15308 = ATCC 14581 AJI21715.1     5
 α-amylase (BmaN3)   Bacillus megaterium NL3 AGT45939.1     5
 AS52_05286   Bacillus megaterium Q3 AKP80214.1     5
 BMQ_5241 (AmyL)   Bacillus megaterium QM B1551 ADE72218.1 D5DXF6   5
 BMWSH_0036   Bacillus megaterium WSH-002 AEN86920.1     5
 ABE28_023605   Bacillus muralis G25-68 AOH57344.1     5
 BG05_2515 (AmyS)   Bacillus mycoides ATCC 6462 AJH21062.1     5
 B7492_18670   Bacillus mycoides Gnyt1 ARJ23075.1     5
 α-amylase (AmyL;BaLi_c07450) 3.2.1.1 Bacillus paralicheniformis ATCC 9945a AGN35141.1     5
 SC10_B2orf01031   Bacillus paralicheniformis BL-09 AJO16877.1     5
 BLMD_03555   Bacillus paralicheniformis MDJK30 ARA87955.1     5
 Bsel_3266   [Bacillus] selenitireducens MLS10 ADI00748.1 D6Y1G1   5
 BS1321_12290   Bacillus simplex NBRC 15720 = DSM 1321 ASS94628.1     5
 UP17_24840   Bacillus simplex SH-B26 AMM95278.1     5
 S101395_00853 (AmyA)   Bacillus sonorensis SRCM101395 ASB87407.1     5
 SP690 (mutant)   Bacillus sp. CAD26698.1
AAE12376.1
AAE37104.1
AAE59723.1
AAE59729.1
AAE62345.1
AAE62351.1
AAN27448.1
AAP02207.1
AAP02213.1
AAR63626.1
AAS26287.1
AAS26293.1
AAY06016.1
AAY72169.1
AAY72175.1
ABJ43098.1
ABN29372.1
ACK07340.1
ACP75065.1
ACW26900.1
CAC88547.1
CAD19031.1
CAD20674.1
CAD67513.1
    5
 unnamed protein product   Bacillus sp. CAD26704.1
AAN18957.1
ABI05898.1
ACS10040.1
CAD26705.1
CAD38132.1
CAV31706.1
CAV31707.1
    5
 Sequence 20 from patent US 6623948   Bacillus sp. AAR63627.1     5
 Sequence 14 from patent US 6623948   Bacillus sp. CAC16494.1
AAR63613.1
AAR63621.1
    5
 Sequence 15 from patent US 6623948   Bacillus sp. CAC16493.1
AAR63612.1
AAR63622.1
    5
 unnamed protein product   Bacillus sp. CAC16487.1
AAN93463.1
AAP02215.1
AAP02216.1
ACK07345.1
ACP75070.1
ACS10036.1
ACW26908.1
CAC16486.1
CAC88552.1
CAD19036.1
CAD20679.1
CAD26703.1
CAD67518.1
CAL48155.1
CAX21647.1
CAX21648.1
CAX63046.1
    5
 α-amylase   Bacillus sp. 1-15 AFP32912.1     5
 CJO35_03350   Bacillus sp. 1s-1 ASV14240.1     5
 α-amylase   Bacillus sp. 406 AFV13099.1     5
 maltohexaose-forming amylase 3.2.1.98 Bacillus sp. 707 AAA22231.1
AAE59728.1
AAE62350.1
AAP02212.1
AAR63620.1
AAS26292.1
AAY06019.1
AAY72174.1
ACK07346.1
ACP75071.1
ACS10032.1
ACW26905.1
P19571 1WP6[A]
1WPC[A]
2D3L[A]
2D3N[A]
5
 unnamed protein product   Bacillus sp. A 7-7 DSM 12368 CAL49846.1     5
 BAQ53_16000   Bacillus sp. B25(2016b) ANP82274.1     5
 α-amylase (AmyB)   Bacillus sp. BDL-10 ABC54846.1 Q2MKJ7   5
 A6J74_27660   Bacillus sp. FDAARGOS_235 ARC32374.1     5
 AM500_14095   Bacillus sp. FJAT-18017 ALC92814.1     5
 BSZ43_03340   Bacillus sp. H15-1 APJ25904.1     5
 AZK53_24970   Bacillus sp. IHB B 7164 ANF48801.1     5
 maltopentaose-producing α-amylase (Amy204) 3.2.1.- Bacillus sp. JAMB-204 BAF03567.1 Q0KKZ7   5
 α-amylase (PRK09441) (fragment) 3.2.1.1 Bacillus sp. KR-8104 AAW29920.1 Q5MB94   5
 alkaline α-amylase (AmyK) 3.2.1.1 Bacillus sp. KSM-1378 AAR68734.1
AAN99906.1
AAR63625.1
AAV01045.1
ABE15847.1
ABI05897.1
ABY03536.1
ACS10038.1
ACW26917.1
BAA32431.1
CAD35985.1
CAD38135.1
CAV31709.1
O82839 2DIE[A] 5
 unnamed protein product   Bacillus sp. KSM-K36 CAC39918.1
ABA16538.1
    5
 α-amylase (AmyK38) 3.2.1.1 Bacillus sp. KSM-K38 CAC39917.1
AAN18958.1
AAV01046.1
ABA16539.1
ABI05896.1
ABY03537.1
ACS10039.1
BAB71820.1
CAD35986.1
CAD38133.1
CAJ00040.1
CAJ28078.1
Q93I48 1UD2[A]
1UD3[A]
1UD4[A]
1UD5[A]
1UD6[A]
1UD8[A]
5
 α-amylase 3.2.1.1 Bacillus sp. MK 716 AAB18785.1 P71034   5
 Sequence 4 from patent US 7078213   Bacillus sp. NCIMB 40916 ABI05900.1
ABI05899.1
    5
 α-amylase (AMY121)   Bacillus sp. SCSIO 15121 AIA26430.1     5
 α-amylase   Bacillus sp. SHG10 AEU12641.1     5
 α-amylase (Amy) 3.2.1.1 Bacillus sp. TS-23 AAA63900.1 Q59222   5
 α-amylase (Amy;BYXA) 3.2.1.1 Bacillus sp. YX / YX-1 ABW87262.1 A9YDD9   5
 KS08_07940   Bacillus subtilis ATCC 13952 AIW35879.1     5
 α-amylase (AmY)   Bacillus subtilis US116 CAL64397.1 A7DWA9   5
 α-amylase   Bacillus subtilis ZJ-1 AFN57616.1     5
 BG06_4468   Bacillus thuringiensis 97-27 AJI34462.1     5
 CAB88_17490   Bacillus thuringiensis ATCC 10792 ARP58752.1
AST01138.1
    5
 BtBc_18025   Bacillus thuringiensis Bc601 AMX78222.1     5
 B7P25_17835   Bacillus thuringiensis BM-BT15426 ARZ63554.1     5
 BMB171_C3160   Bacillus thuringiensis BMB171 ADH07970.1 D5TT57   5
 BTI247_37200 (AmyS)   Bacillus thuringiensis Bt18247 AOM12102.1     5
 AXW78_16430   Bacillus thuringiensis Bt185 AMR03633.1     5
 BTB_c35500 (AmyS)   Bacillus thuringiensis Bt407 AFV19232.1     5
 ATN06_17425   Bacillus thuringiensis CTC ALQ69061.1     5
 BTG_02170   Bacillus thuringiensis HD-771 AFQ13935.1     5
 BTF1_14685   Bacillus thuringiensis HD-789 AFQ27116.1     5
 AS86_568 (AmyS)   Bacillus thuringiensis HD1002 AJH06613.1     5
 BF38_4648 (AmyS)   Bacillus thuringiensis HD1011 AJG76694.1     5
 A3L20_18230   Bacillus thuringiensis HD12 AMR85878.1     5
 A3L20_18230   Bacillus thuringiensis HD12 AMR85878.1     5
 BF32_1100 (AmyS)   Bacillus thuringiensis HD571 AJH69278.1     5
 BF36_1581 (AmyS)   Bacillus thuringiensis HD682 AJH80994.1     5
 AC241_17015   Bacillus thuringiensis HS18-1 AKR10353.1     5
 BCM43_11840   Bacillus thuringiensis KNU-07 ANV71168.1     5
 B4918_19300   Bacillus thuringiensis L-7601 AQY39954.1     5
 MC28_2635   Bacillus thuringiensis MC28 AFU14057.1     5
 BT246_37660 (AmyS)   Bacillus thuringiensis MYBT18246 ANS49114.1     5
 BJG91_13445   Bacillus thuringiensis SCG04-02 ARV93569.1     5
 Bt4C1_16885   Bacillus thuringiensis serovar alesti BGSC 4C1 AND08805.1     5
 CT43_CH3417 (AmyS)   Bacillus thuringiensis serovar chinensis CT-43 AEA17084.1     5
 A9498_17925   Bacillus thuringiensis serovar coreanensis ST7 ANN33271.1     5
 BT4G5_18065   Bacillus thuringiensis serovar galleriae HD-29 AJA20681.1     5
 NF53_3346 (AmyS)   Bacillus thuringiensis serovar indiana HD521 AKR36424.1     5
 ATN07_17040   Bacillus thuringiensis serovar israelensis AM65-52 AND25200.1     5
 BT9727_3261 (AmyS)   [Bacillus thuringiensis] serovar konkukian str. 97-27 AAT60457.1 Q6HFU3   5
 BG08_2370   Bacillus thuringiensis serovar kurstaki HD 1 AJK40726.1     5
 BTK_18365   Bacillus thuringiensis serovar kurstaki str. HD-1 AIE34725.1     5
 HD73_3728   Bacillus thuringiensis serovar kurstaki str. HD73 AGE79306.1     5
 DF16_orf02509   Bacillus thuringiensis serovar kurstaki str. YBT-1520 AIM30924.1     5
 YBT1520_18375   Bacillus thuringiensis serovar kurstaki str. YBT-1520 AHZ52300.1     5
 SD98_17925   Bacillus thuringiensis serovar morrisoni BGSC 4AA1 AJQ60085.1     5
 H175_ch3471   Bacillus thuringiensis serovar thuringiensis str. IS5056 AGG02183.1     5
 KNN_02439   Bacillus thuringiensis serovar tolworthi Pasteur Institute Standard strain BAR83285.1     5
 BALH_3143   Bacillus thuringiensis str. Al Hakam ABK86395.1 A0RGQ2   5
 BTXL6_27010   Bacillus thuringiensis XL6 ALL24901.1     5
 YBT1518_18920   Bacillus thuringiensis YBT-1518 AHA72930.1     5
 XI92_25465   Bacillus thuringiensis YC-10 AKJ61404.1     5
 BVH75_15025   Bacillus thuringiensis YGD22-03 ARX67265.1     5
 AQ980_12680   Bacillus thuringiensis YWC2-8 ALL58670.1     5
 Btoyo_0702   Bacillus toyonensis BCT-7112 AHA06710.1     5
 CHN56_00589   Bacillus velezensis SCDB 291 ASS61133.1     5
 CLI97_03658   Bacillus velezensis SCGB 1 ATC52882.1     5
 S100072_01694   Bacillus velezensis SRCM100072 ASB53030.1     5
 BcerKBAB4_3200   Bacillus weihenstephanensis KBAB4 ABY44376.1
ACH26093.1
A9VMV3   5
 bwei_1451 (AmyS)   Bacillus weihenstephanensis WSBC 10204 AIW84104.1     5
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