Glycoside Hydrolase Family 13 / Subf 5

Activities in Familyα-amylase (EC 3.2.1.1); pullulanase (EC 3.2.1.41); cyclomaltodextrin glucanotransferase (EC 2.4.1.19); cyclomaltodextrinase (EC 3.2.1.54); trehalose-6-phosphate hydrolase (EC 3.2.1.93); oligo-α-glucosidase (EC 3.2.1.10); maltogenic amylase (EC 3.2.1.133); neopullulanase (EC 3.2.1.135); α-glucosidase (EC 3.2.1.20); maltotetraose-forming α-amylase (EC 3.2.1.60); isoamylase (EC 3.2.1.68); glucodextranase (EC 3.2.1.70); maltohexaose-forming α-amylase (EC 3.2.1.98); maltotriose-forming α-amylase (EC 3.2.1.116); branching enzyme (EC 2.4.1.18); trehalose synthase (EC 5.4.99.16); 4-α-glucanotransferase (EC 2.4.1.25); maltopentaose-forming α-amylase (EC 3.2.1.-) ; amylosucrase (EC 2.4.1.4) ; sucrose phosphorylase (EC 2.4.1.7); malto-oligosyltrehalose trehalohydrolase (EC 3.2.1.141); isomaltulose synthase (EC 5.4.99.11); malto-oligosyltrehalose synthase (EC 5.4.99.15); amylo-α-1,6-glucosidase (EC 3.2.1.33); α-1,4-glucan: phosphate α-maltosyltransferase (EC 2.4.99.16); 6'-P-sucrose phosphorylase (EC 2.4.1.-); amino acid transporter
Activities in Sub Family
Mechanism Retaining
ClanGH-H
3D Structure Status( β / α ) 8
Catalytic Nucleophile/BaseAsp (experimental)
Catalytic Proton DonorGlu (experimental)
NoteNew: many members have been assigned to subfamilies as described by Stam et al. (2006) Protein Eng Des Sel. 19, 555-562 (PMID: 17085431)
External resourcesCAZypedia; EBI Protein of the Month; HOMSTRAD; PDB Molecule of the Month; PRINTS;
Commercial Enzyme Provider(s)MEGAZYME; PROZOMIX;
Statistics GenBank accession (2341); Uniprot accession (238); PDB accession (24); 3D entries (8); cryst (0)
Bacteria
Protein Name EC# OrganismGenBankUniprot
PDB/3D Carbohydrate Ligands Resolution (Å)
Subf
 α-amylase (Amy1;AmyQ;BAA) 3.2.1.1 Bacillus amyloliquefaciens AAA22191.1
AAE12381.1
AAE13947.1
AAE37101.1
AAE59727.1
AAE62349.1
AAN20003.1
AAN27445.1
AAN99911.1
AAP02211.1
AAR63623.1
AAR76030.1
AAS26291.1
AAY72173.1
ABJ43095.1
ABJ43099.1
ABN29369.1
ACK07344.1
ACP75069.1
ACS10035.1
ACW26904.1
CAA01489.1
CAA23430.1
CAC16484.1
CAC88551.1
CAD19035.1
CAD20678.1
CAD26702.1
CAD67517.1
CAD88112.1
P00692
3BH4[A,B] 1.40
5
 α-amylase 3.2.1.1 Bacillus halmapalus CAD26699.1
AAE12377.1
AAE59724.1
AAE59730.1
AAE62346.1
AAE62352.1
AAP02208.1
AAP02214.1
AAS26288.1
AAS26294.1
AAY06017.1
AAY72170.1
AAY72176.1
ACK07341.1
ACP75066.1
ACS10037.1
ACW26901.1
CAC88548.1
CAD19032.1
CAD20675.1
CAD67514.1
 
1W9X[A] <non_carb>-(1-4)-α-D-6-deoxy-Glcp4N-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-<non_carb>-(0-4)-α-D-6-deoxy-Glcp4N-(1-4)-α-D-Glcp-(1-4)-β-D-Glcp
2.1
2GJP[A] <non_carb>-(1-4)-α-D-6-deoxy-Glcp4N-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-<non_carb>-(0-4)-α-D-6-deoxy-Glcp4N-(1-4)-α-D-Glcp-(1-4)-β-D-Glcp
D-1-deoxy-Glcp
α-D-Glcp-(1-4)-α-D-Glcp
1.90
2GJR[A] 2.10
5
 α-amylase (AmyA;AmyL;AmyS;BLA) 3.2.1.1 Bacillus licheniformis 584 / ATCC 27811 AAA22226.1
AAA22193.1
AAA22240.1
AAN99913.1
AAO26743.1
AAT46561.1
ACM17155.1
CAA01355.1
CAA03075.1
CAA26981.1
CAC16483.1
CAC88550.1
CAD19034.1
CAD20677.1
CAD26701.1
CAD26706.1
CAD88111.1
CAV31708.1
P06278
Q6GWE2
1BLI[A] 1.90
1BPL[A,B] 2.20
1OB0[A] 1.83
1VJS[A] 1.70
5
 maltohexaose-forming amylase 3.2.1.98 Bacillus sp. 707 AAA22231.1
AAE59728.1
AAE62350.1
AAP02212.1
AAR63620.1
AAS26292.1
AAY06019.1
AAY72174.1
ACK07346.1
ACP75071.1
ACS10032.1
ACW26905.1
P19571
1WP6[A] 2.10
1WPC[A] <non_carb>-(1-4)-α-D-6-deoxy-Glcp4N-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-<non_carb>-(0-4)-α-D-6-deoxy-Glcp4N-(1-4)-α-D-Glcp-(1-4)-β-D-Glcp
α-D-Glcp-(1-4)-α-D-Glcp
1.90
2D3L[A] α-D-Glcp
α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp
α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp
2.30
2D3N[A] α-D-Glcp
α-D-Glcp-(1-4)-α-D-Glcp
α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp
α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp
α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp
1.90
5
 alkaline α-amylase (AmyK) 3.2.1.1 Bacillus sp. KSM-1378 AAR68734.1
AAN99906.1
AAR63625.1
AAV01045.1
ABE15847.1
ABI05897.1
ABY03536.1
ACS10038.1
ACW26917.1
BAA32431.1
CAD35985.1
CAD38135.1
CAV31709.1
O82839
2DIE[A] 2.10
5
 α-amylase (AmyK38) 3.2.1.1 Bacillus sp. KSM-K38 CAC39917.1
AAN18958.1
AAV01046.1
ABA16539.1
ABI05896.1
ABY03537.1
ACS10039.1
BAB71820.1
CAD35986.1
CAD38133.1
CAJ00040.1
CAJ28078.1
Q93I48
1UD2[A] 2.13
1UD3[A] 2.15
1UD4[A] 2.15
1UD5[A] 2.70
1UD6[A] 2.50
1UD8[A] 2.88
5
 α-amylase (AmyS) 3.2.1.1 Geobacillus stearothermophilus DY5 / PHI300 / NZ-3 AAA22235.2
AAA22227.1
AAA22241.1
AAE13948.1
AAE37102.1
AAE59725.1
AAE62347.1
AAN20004.1
AAN27446.1
AAP02209.1
AAR63624.1
AAS26289.1
AAY06018.1
AAY72171.1
ABJ43096.1
ABN29370.1
ACK07342.1
ACP75067.1
ACW26902.1
CAA26547.1
CAC16485.1
CAC88549.1
CAD19033.1
CAD20676.1
CAD26700.1
CAD67515.1
P06279
1HVX[A] 2.00
4UZU[A] 1.90
5
 α-amylase B (AmyB) 3.2.1.1 Halothermothrix orenii H 168 ACL70573.1 B8CZ54
3BC9[A] <non_carb>-(1-4)-α-D-6-deoxy-Glcp4N-(1-4)-α-D-Glcp-(1-4)-β-D-Glcp
α-D-6-deoxy-Glcp-(1-4)-α-D-Glcp-(1-4)-<non_carb>-(0-4)-α-D-6-deoxy-Glcp4N-(1-4)-α-D-Glcp-(1-4)-<non_carb>-(0-4)-α-D-6-deoxy-Glcp4N-(1-4)-α-D-Glcp-(1-4)-β-D-Glcp
α-D-Glcp
1.35
3BCD[A] α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp
α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-β-D-Glcp
a-cyclodextrin
2.20
3BCF[A] 2.30
5
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