Glycoside Hydrolase Family 13 / Subf 5

Activities in Familyα-amylase (EC 3.2.1.1); pullulanase (EC 3.2.1.41); cyclomaltodextrin glucanotransferase (EC 2.4.1.19); cyclomaltodextrinase (EC 3.2.1.54); trehalose-6-phosphate hydrolase (EC 3.2.1.93); oligo-α-glucosidase (EC 3.2.1.10); maltogenic amylase (EC 3.2.1.133); neopullulanase (EC 3.2.1.135); α-glucosidase (EC 3.2.1.20); maltotetraose-forming α-amylase (EC 3.2.1.60); isoamylase (EC 3.2.1.68); glucodextranase (EC 3.2.1.70); maltohexaose-forming α-amylase (EC 3.2.1.98); maltotriose-forming α-amylase (EC 3.2.1.116); branching enzyme (EC 2.4.1.18); trehalose synthase (EC 5.4.99.16); 4-α-glucanotransferase (EC 2.4.1.25); maltopentaose-forming α-amylase (EC 3.2.1.-) ; amylosucrase (EC 2.4.1.4) ; sucrose phosphorylase (EC 2.4.1.7); malto-oligosyltrehalose trehalohydrolase (EC 3.2.1.141); isomaltulose synthase (EC 5.4.99.11); malto-oligosyltrehalose synthase (EC 5.4.99.15); amylo-α-1,6-glucosidase (EC 3.2.1.33); α-1,4-glucan: phosphate α-maltosyltransferase (EC 2.4.99.16); 6'-P-sucrose phosphorylase (EC 2.4.1.-); amino acid transporter
Activities in Sub Family
Mechanism Retaining
ClanGH-H
3D Structure Status( β / α ) 8
Catalytic Nucleophile/BaseAsp (experimental)
Catalytic Proton DonorGlu (experimental)
NoteNew: many members have been assigned to subfamilies as described by Stam et al. (2006) Protein Eng Des Sel. 19, 555-562 (PMID: 17085431)
External resourcesCAZypedia; EBI Protein of the Month; HOMSTRAD; PDB Molecule of the Month; PRINTS;
Commercial Enzyme Provider(s)MEGAZYME; PROZOMIX;
Statistics GenBank accession (2341); Uniprot accession (238); PDB accession (24); 3D entries (8); cryst (0)
Bacteria
Protein Name EC#OrganismGenBank UniprotPDB/3DSubf
 α-amylase (Amy1;AmyA;AmyB) 3.2.1.1 Alkalimonas amylolytica N10 AAQ01675.1 Q6WUB6   5
 α-amylase (AFA;AMY1048) 3.2.1.1 Anoxybacillus flavithermus CAL14744.1     5
 α-amylase (fragment) 3.2.1.1 Bacillus acidicola TSAS1 AEW07376.1     5
 α-amylase (Amy1;AmyQ;BAA) 3.2.1.1 Bacillus amyloliquefaciens AAA22191.1
AAE12381.1
AAE13947.1
AAE37101.1
AAE59727.1
AAE62349.1
AAN20003.1
AAN27445.1
AAN99911.1
AAP02211.1
AAR63623.1
AAR76030.1
AAS26291.1
AAY72173.1
ABJ43095.1
ABJ43099.1
ABN29369.1
ACK07344.1
ACP75069.1
ACS10035.1
ACW26904.1
CAA01489.1
CAA23430.1
CAC16484.1
CAC88551.1
CAD19035.1
CAD20678.1
CAD26702.1
CAD67517.1
CAD88112.1
P00692 3BH4[A,B] 5
 α-amylase 3.2.1.1 Bacillus amyloliquefaciens ATCC 23842 ADE44086.1 D5KR60   5
 α-amylase (BCA) 3.2.1.1 Bacillus cereus GUF8 ABY86223.1 B0LVG1   5
 α-amylase 3.2.1.1 Bacillus halmapalus CAD26699.1
AAE12377.1
AAE59724.1
AAE59730.1
AAE62346.1
AAE62352.1
AAP02208.1
AAP02214.1
AAS26288.1
AAS26294.1
AAY06017.1
AAY72170.1
AAY72176.1
ACK07341.1
ACP75066.1
ACS10037.1
ACW26901.1
CAC88548.1
CAD19032.1
CAD20675.1
CAD67514.1
  1W9X[A]
2GJP[A]
2GJR[A]
5
 Sequence from patent 3.2.1.1 Bacillus licheniformis AAE13946.1
AAE37100.1
AAE59726.1
AAE62348.1
AAN27444.1
AAP02210.1
AAR63628.1
AAR76029.1
AAS26290.1
ABA38471.1
ABJ43094.1
ABN29368.1
ACK07343.1
ACP75068.1
ACS10034.1
ACW26903.1
CAD38134.1
CAD67516.1
    5
 α-amylase (Am-Lich) 3.2.1.1 Bacillus licheniformis AAE12380.1     5
 α-amylase 3.2.1.1 Bacillus licheniformis AAE12379.1
ABA38472.1
ACS10033.1
    5
 α-amylase (AmyL) 3.2.1.1 Bacillus licheniformis 44MB82/G AGR34077.1     5
 α-amylase (AmyA;AmyL;AmyS;BLA) 3.2.1.1 Bacillus licheniformis 584 / ATCC 27811 AAA22226.1
AAA22193.1
AAA22240.1
AAN99913.1
AAO26743.1
AAT46561.1
ACM17155.1
CAA01355.1
CAA03075.1
CAA26981.1
CAC16483.1
CAC88550.1
CAD19034.1
CAD20677.1
CAD26701.1
CAD26706.1
CAD88111.1
CAV31708.1
P06278
Q6GWE2
1BLI[A]
1BPL[A,B]
1OB0[A]
1VJS[A]
5
 α-amylase (AmyL) 3.2.1.1 Bacillus licheniformis B0204 ABD66592.1 Q208A7   5
 α-amylase (thermostable) 3.2.1.1 Bacillus licheniformis CICC 10181 ACN88150.1 C0LZX2   5
 α-amylase (Amy;BLi00656;BL00499) 3.2.1.1 Bacillus licheniformis DSM 13 = ATCC 14580 AAU39594.1
AAU22245.1
Q65MX0   5
 α-amylase (AmyB) 3.2.1.1 Bacillus licheniformis MD1 CAH10338.1 P06278
Q6GWE2
  5
 α-amylase 3.2.1.1 Bacillus licheniformis NCIB8061 AAE12384.1     5
 α-amylase (AmyN) 3.2.1.1 Bacillus licheniformis NH1 ABL75259.1 A1YR25   5
 maltopentaose-forming α-amylase 3.2.1.- Bacillus megaterium KSM B-404 AAK00598.1 Q9AQ54   5
 α-amylase (AmyL;BaLi_c07450) 3.2.1.1 Bacillus paralicheniformis ATCC 9945a AGN35141.1     5
 maltohexaose-forming amylase 3.2.1.98 Bacillus sp. 707 AAA22231.1
AAE59728.1
AAE62350.1
AAP02212.1
AAR63620.1
AAS26292.1
AAY06019.1
AAY72174.1
ACK07346.1
ACP75071.1
ACS10032.1
ACW26905.1
P19571 1WP6[A]
1WPC[A]
2D3L[A]
2D3N[A]
5
 maltopentaose-producing α-amylase (Amy204) 3.2.1.- Bacillus sp. JAMB-204 BAF03567.1 Q0KKZ7   5
 α-amylase (PRK09441) (fragment) 3.2.1.1 Bacillus sp. KR-8104 AAW29920.1 Q5MB94   5
 alkaline α-amylase (AmyK) 3.2.1.1 Bacillus sp. KSM-1378 AAR68734.1
AAN99906.1
AAR63625.1
AAV01045.1
ABE15847.1
ABI05897.1
ABY03536.1
ACS10038.1
ACW26917.1
BAA32431.1
CAD35985.1
CAD38135.1
CAV31709.1
O82839 2DIE[A] 5
 α-amylase (AmyK38) 3.2.1.1 Bacillus sp. KSM-K38 CAC39917.1
AAN18958.1
AAV01046.1
ABA16539.1
ABI05896.1
ABY03537.1
ACS10039.1
BAB71820.1
CAD35986.1
CAD38133.1
CAJ00040.1
CAJ28078.1
Q93I48 1UD2[A]
1UD3[A]
1UD4[A]
1UD5[A]
1UD6[A]
1UD8[A]
5
 α-amylase 3.2.1.1 Bacillus sp. MK 716 AAB18785.1 P71034   5
 α-amylase (Amy) 3.2.1.1 Bacillus sp. TS-23 AAA63900.1 Q59222   5
 α-amylase (Amy;BYXA) 3.2.1.1 Bacillus sp. YX / YX-1 ABW87262.1 A9YDD9   5
 α-amylase (RSDA) (raw-starch-digesting) 3.2.1.1 Cytophaga sp. AAF00567.1 Q9RQT8   5
 α-amylase 2 (AmyAl;EAMY) 3.2.1.1 Escherichia coli str. K-12 substr. MG1655 AAC74994.1
AAA23810.1
AAA82575.1
AIZ90980.1
ALI40306.1
AMC94904.1
NP_416437.1
P26612   5
 α-amylase (AmyH) 3.2.1.1 Exiguobacterium sp. DAU5 AFZ41193.1     5
 α-amylase (Am-Stearo) 3.2.1.1 Geobacillus stearothermophilus AAE12382.1     5
 α-amylase (Ami;Amy;AmyUS100) 3.2.1.1
3.2.1.98
Geobacillus stearothermophilus US100 / ATCC 31195 AAB86961.1
AAN99912.1
ACH26104.1
CAB93517.1
CCB84710.1
O31193
Q9KWY6
  5
 α-amylase (AmyS) 3.2.1.1 Geobacillus stearothermophilus DY5 / PHI300 / NZ-3 AAA22235.2
AAA22227.1
AAA22241.1
AAE13948.1
AAE37102.1
AAE59725.1
AAE62347.1
AAN20004.1
AAN27446.1
AAP02209.1
AAR63624.1
AAS26289.1
AAY06018.1
AAY72171.1
ABJ43096.1
ABN29370.1
ACK07342.1
ACP75067.1
ACW26902.1
CAA26547.1
CAC16485.1
CAC88549.1
CAD19033.1
CAD20676.1
CAD26700.1
CAD67515.1
P06279 1HVX[A]
4UZU[A]
5
 α-amylase 3.2.1.1 Geobacillus stearothermophilus GRE1 ABC18196.1     5
 α-amylase 3.2.1.1 Geobacillus stearothermophilus TISTR 329 AAU10478.1 Q66T78   5
 α-amylase (GA) 3.2.1.1 Geobacillus thermodenitrificans LH8 ABX83871.1 A9UJ60   5
 α-amylase (Amy;Gt-Amy) 3.2.1.1 Geobacillus thermoleovorans NP54 AFC87833.1     5
 α-amylase B (AmyB) 3.2.1.1 Halothermothrix orenii H 168 ACL70573.1 B8CZ54 3BC9[A]
3BCD[A]
3BCF[A]
5
 α-amylase (Amy1) 3.2.1.1 Nostoc sp. PCC 7119 CAQ30277.1 B1VK33   5
 α-amylase 2 (AmyA) 3.2.1.1 Salmonella typhimurium SJW1103 AAA27110.1
AAA27079.1
AAA71970.1
P26613   5
 α-amylase II (Amy II) 3.2.1.1 Streptococcus equinus 148 BAA24178.1 O50583   5
 α-amylase 3.2.1.1 Streptococcus equinus JB1 AAA97431.1 Q53786   5
 α-amylase (intracellular) (Amy) 3.2.1.1 Streptococcus mutans LT11 AAC35010.1 O68875   5
 α-amylase (AmyA;SMU.1590) 3.2.1.1 Streptococcus mutans UA159 AAN59233.1
NP_721927.1
    5
 α-amylase (Amy13A) 3.2.1.1 uncultured bacterium CCD30600.1     5
 α-amylase (Amy63) 3.2.1.1 Vibrio alginolyticus 129-63 ALP73597.1     5
Eukaryota
Protein Name EC#OrganismGenBank UniprotPDB/3DSubf
 maltotriose-producing α-amylase (AmyD;An01g13610) (intracellular) 3.2.1.1
3.2.1.116
Aspergillus niger CBS 513.88 CAK37367.1 A2QB23   5
 cell-wall active enzyme / α-amylase (Amy1;PbrAmy1) 2.4.1.-
3.2.1.1
Paracoccidioides brasiliensis IVIC Pb73 / ATCC 3207 ABS11196.1 A7L832   5
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