Activities in Sub Family	[retaining] α-amylase (EC 3.2.1.1); [retaining] pullulanase (EC 3.2.1.41);
Mechanism	Retaining a
Clan	GH-H
3D Structure Status	( β / α ) 8 barrel
Catalytic Nucleophile/Base	Asp (experimental)
Catalytic Proton Donor	Glu (experimental)
Note	New: many members have been assigned to subfamilies as described by Stam et al. (2006) Protein Eng Des Sel. 19, 555-562 (PMID: 17085431)
External resources	CAZypedia; EBI Protein of the Month; HOMSTRAD; PDB Molecule of the Month; PRINTS;
Commercial Enzyme Provider(s)	MEGAZYME; PROZOMIX;
Statistics	GenBank accession (6); Uniprot accession (12);

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Bacteria
Protein Name	EC#	Organism	GenBank	Uniprot	PDB/3D	Subf
JBW_00429		Pelosinus fermentans JBW45	AJQ25781.1			39
UFO1_3575		Pelosinus sp. UFO1	AIF53118.1			39
UFO1_4375		Pelosinus sp. UFO1	AIF53918.1			39
EAL2_c09190 (ApU)		Peptoclostridium acidaminophilum DSM 3953	AHM56218.1			39
WG909_00535		Peptostreptococcaceae bacterium AGR-M142	WXR61728.1			39
GAI11004_07570		Peptostreptococcus porci GAI11004	BFF72819.1			39
EQG49_07740		Periweissella cryptocerci 26KH-42	QBO36360.1			39
PATL70BA_2228		Petrocella atlantisensis 70B-A	VDN48119.1			39
QNI29_20555		Pontibacillus chungwhensis HN14s	WIF98083.1			39
IMZ31_18450		Pontibacillus sp. ALD_SL1	QST00011.1			39
IE339_05025		Priestia koreensis FS-1	UNL85874.1			39
HFZ78_08100		Priestia megaterium S2	QIZ06675.1			39
P6M73_09690		Proteiniclasticum sp. QWL-01	WFF71586.1			39
LC071_10175 (fragment)		Pseudalkalibacillus hwajinpoensis KcN21-2	WLR61617.1			39
LC071_10180 (fragment)		Pseudalkalibacillus hwajinpoensis KcN21-2	WLR61618.1			39
MM59RIKEN_14380		Pusillibacter faecalis MM59	BCK84119.1			39
FRIFI_0370		Romboutsia hominis FRIFI	CEI71918.1			39
CRIB_296		Romboutsia ilealis CRIB	CED93053.1			39
HF520_02125		Romboutsia sp. CE17	QJA07813.1			39
RD2015_3666		Roseateles depolymerans KCTC 42856	ALV08121.1			39
NQ522_06820		Roseburia intestinalis L1 82	UWP56928.1			39
RIL182_01368 (ApU)		Roseburia intestinalis L1-82	VCV21496.1			39
ROI_28570		Roseburia intestinalis M50/1	CBL09766.1			39
M5E85_06550 (fragment)		Roseburia intestinalis NB2A 31_NB	UQT31903.1			39
RO1_38940		Roseburia intestinalis XB6B4	CBL14115.1			39
H8S51_007010 (fragment)		Roseburia rectibacter NSJ-69	UMZ01448.1			39
H8S51_006995 (fragment)		Roseburia rectibacter NSJ-69	UMZ01445.1			39
Rcas_2327		Roseiflexus castenholzii DSM 13941	ABU58410.1			39
RoseRS_2452		Roseiflexus sp. RS-1	ABQ90829.1			39
B6259_01440		Ruminococcaceae bacterium CPB6	ARP49674.1			39
B6259_00305		Ruminococcaceae bacterium CPB6	ARP49467.1			39
GKP14_04470		Ruminococcaceae bacterium JNU-WLY1368	QKO30334.1			39
GKP14_03795		Ruminococcaceae bacterium JNU-WLY1368	QKO30210.1			39
E5Z56_04705		Ruminococcus bovis JE7A12	QCT06708.1			39
RBR_06100 (Amy3)		Ruminococcus bromii L2-63	CBL14980.1			39
LCN94_00230		Ruminococcus sp. FMB-CY1	WBX57568.1			39
KGF34_08090		Ruminococcus sp. FMBCY1	USP69132.1			39
U0355_01385		Salimicrobium sp. PL1-032A	WVR01961.1			39
LH991_01525		Schleiferilactobacillus harbinensis LH991	QFR65430.1			39
FMM01_07815		Schleiferilactobacillus harbinensis NSMJ42	QEU48804.1			39
RBQ61_04395		Sedimentibacter sp. MB35-C1	WMJ78180.1			39
P3F81_00515 (MalQ)		Selenobaculum gbiensis ICN-92133	WIW70840.1			39
QU667_02730 (MalQ)		Selenomonas dianae ATCC 43527	WLD82906.1			39
HMPREF9334_01916		Selenomonas infelix ATCC 43532	EHG18778.1			39
SELR_04420		Selenomonas ruminantium subsp. lactilytica TAM6421	BAL82150.1			39
SELR_27160		Selenomonas ruminantium subsp. lactilytica TAM6421	BAL84424.1			39
AXF19_00495		Selenomonas sp. oral taxon 126 W7667	ANR69627.1			39
AXE86_09270		Selenomonas sp. oral taxon 136 F0591	AME04250.1			39
ADJ74_01050		Selenomonas sp. oral taxon 478 F0592	AKT53142.1			39
BCS37_02625		Selenomonas sp. oral taxon 920 W5150	AOH47405.1			39
TAMA11512_07450		Selenomonas sp. TAMA-11512	BEU87281.1			39
Selsp_2025		Selenomonas sputigena ATCC 35185	AEC00974.1			39
OL236_09305 (MalQ)		Selenomonas sputigena KCOM 1787	UZE44776.1			39
OL240_06810 (MalQ)		Selenomonas sputigena KCOM 20461	UZD42259.1			39
H1B31_05975 (MalQ)		Selenomonas timonae Marseille-Q3039	QNH53465.1			39
Shel_17020		Slackia heliotrinireducens DSM 20476	ACV22721.1			39
NCTC11029_01611 (ApU)		Slackia heliotrinireducens NCTC11029	VEH01348.1			39
St703_16230 (ApU)		Sporolactobacillus terrae 70-3	BBN98918.1			39
C0674_08125		Sporolactobacillus terrae DLG3	QAA22596.1			39
C0679_08105		Sporolactobacillus terrae DRG1	QAA25569.1			39
K7399_05430		Sporolactobacillus terrae EFEL7003	UAK17378.1			39
SOV_00840 (MalQ)		Sporomusa ovata DSM 2662	WWV68858.1			39
SPSPH_001150		Sporomusa sphaeroides DSM 2875	WXA37451.1			39
SPTER_46970		Sporomusa termitida DSM 4440	QDR83216.1			39
OZX60_06285		Streptococcaceae bacterium ESL0687	WEV45039.1			39
OZX68_06335		Streptococcaceae bacterium ESL0729	WEV60533.1			39
NQ490_02890		Subdoligranulum variabile DSM 15176	UWP68815.1			39
TEGL_35750 (ApU)		Terrisporobacter glycolicus ATCC 14880 = DSM 1288	WWD85121.1			39
L0P85_16180		Terrisporobacter glycolicus WW3900	UPA30093.1			39
JW646_11575		Terrisporobacter hibernicus MCA3	UEL46291.1			39
TEMA_24630 (ApU)		Terrisporobacter mayombei DSM 6539	WMT82105.1			39
AC806_05920		Tetragenococcus halophilus MJ4	AOF48958.1			39
KFZ55_05555		Tetragenococcus halophilus SNTH-1	WJS83002.1			39
C7K43_04045		Tetragenococcus koreensis KCTC 3924	AYW45175.1			39
C7K38_10285		Tetragenococcus osmophilus JCM 31126	AYW48729.1			39
BR63_00825		Thermanaerosceptrum fracticalcis DRI-13	QNB44994.1			39
amylopullulanase (ApU)		Thermoanaerobacter brockii subsp. brockii	UBS28759.1			39
Thebr_2219		Thermoanaerobacter brockii subsp. finnii Ako-1	ADV80725.1			39
Thit_0062		Thermoanaerobacter italicus Ab9	ADD01391.1			39
Tmath_0121		Thermoanaerobacter mathranii subsp. mathranii str. A3	ADH59906.1			39
amylase-pullulanase (Apu;Apu-Te;TrAPU;Teth39_2173)	3.2.1.1 3.2.1.41	Thermoanaerobacter pseudethanolicus ATCC 33223	ABY95795.1 AAA23201.1 AAV00812.1	P38939 P38939		39
OEI98_000089		Thermoanaerobacter sp. RKWS2	UZQ83090.1			39
Thet_0070		Thermoanaerobacter sp. X513	ADN53741.1			39
Teth514_1781		Thermoanaerobacter sp. X514	ABY93066.1			39
amylase-pullulanase (Apu)	3.2.1.1 3.2.1.41	Thermoanaerobacter thermohydrosulfuricus	AAA23205.1 CAZ78897.1	P16950		39
Thewi_0084		Thermoanaerobacter wiegelii Rt8.B1	AEM77594.1			39
amylase-pullulanase (Apu;Apu-Ts)	3.2.1.1 3.2.1.41	Thermoanaerobacterium saccharolyticum B6A-RI	AAA19800.1	P36905		39
amylopullulanase, partial (ApU)		Thermoanaerobacterium saccharolyticum DSM 7060 B6A-RI	AAA19800.2			39
Tsac_1342		Thermoanaerobacterium saccharolyticum JW/SL-YS485	AFK86349.1			39
amylopullulanase (Apu;Apu-Ts) (partial)	3.2.1.1 3.2.1.41	Thermoanaerobacterium saccharolyticum NTOU1	ACR15165.1	C5IGQ7		39
Q2T46_12525		Thermoanaerobacterium sp. CMT5567-10	WKV08348.1			39
SAMN05660242_1449		Thermoanaerobacterium sp. RBIITD	SNX53839.1			39
Tthe_0751		Thermoanaerobacterium thermosaccharolyticum DSM 571	ADL68306.1			39
amylase-pullulanase (AmyB)	3.2.1.1 3.2.1.41	Thermoanaerobacterium thermosulfurigenes EM1	AAB00841.1	P38536		39
Thexy_0614		Thermoanaerobacterium xylanolyticum LX-11	AEF16665.1			39
Tter_2278		Thermobaculum terrenum ATCC BAA-798	ACZ43177.1			39
INP52_09305		Thermophilibacter immobilis LZLJ-2	QOY60572.1			39
KDW03_05285 (fragment)		Thermospira aquatica F1F22	URA11209.1			39
Firmicute1046_0860		uncultured Firmicutes bacterium	QGT51010.1			39
H9L18_08505		Vagococcus carniphilus ATCC BAA-640	QNN71933.1			39
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