Activities in Sub Family	[retaining] α-amylase (EC 3.2.1.1); α-glucosidase (EC 3.2.1.20); [retaining] pullulanase (EC 3.2.1.41); Isoamylase (EC 3.2.1.68);
Mechanism	Retaining a
Clan	GH-H
3D Structure Status	( β / α ) 8 barrel
Catalytic Nucleophile/Base	Asp (experimental)
Catalytic Proton Donor	Glu (experimental)
Note	New: many members have been assigned to subfamilies as described by Stam et al. (2006) Protein Eng Des Sel. 19, 555-562 (PMID: 17085431)
External resources	CAZypedia; EBI Protein of the Month; HOMSTRAD; PDB Molecule of the Month; PRINTS;
Commercial Enzyme Provider(s)	MEGAZYME; PROZOMIX;
Statistics	GenBank accession (9); Uniprot accession (39); PDB accession (25); 3D entries (6); cryst (1)

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Bacteria
Protein Name	EC#	Organism	GenBank	Uniprot	PDB/3D	Subf
SP70585_1189 (PulA)		Streptococcus pneumoniae 70585	ACO17708.1			14
T308_05425		Streptococcus pneumoniae A026	AGZ47821.1			14
A66_01022		Streptococcus pneumoniae A66	CRI61733.1			14
SPAP_1075		Streptococcus pneumoniae AP200	ADM84664.1			14
ASP0581_10400 (PulI)		Streptococcus pneumoniae ASP0581	BBG81562.1			14
SPAT_1049 (AmyX)		Streptococcus pneumoniae ATCC 49619	BBG39101.1			14
SPN23F10390		Streptococcus pneumoniae ATCC 700669	CAR68858.1			14
GJE16_05240 (PulA)		Streptococcus pneumoniae AUSMDU00010538	QGK03862.1			14
SPCG_1162 (PulI)		Streptococcus pneumoniae CGSP14	ACB90414.1			14
DA395_05635 (PulA)		Streptococcus pneumoniae CP2215	AVV91355.1			14
SPND122_01102 (fragment)		Streptococcus pneumoniae D122	AOG56003.1			14
SPND122_01103 (fragment)		Streptococcus pneumoniae D122	AOG56004.1			14
SPND141_01104		Streptococcus pneumoniae D141	AOG58132.1			14
SPD_1002		Streptococcus pneumoniae D39	ABJ53658.1			14
SPV_1002 (PulA)		Streptococcus pneumoniae D39V	AVN86043.1			14
C3Y86_05630 (PulA)		Streptococcus pneumoniae EF3030	AXK91515.1 QBF69035.1			14
SPG_1036 (fragment)		Streptococcus pneumoniae G54	ACF55060.1			14
HMPREF1038_01061		Streptococcus pneumoniae gamPNI0373	AFS43111.1			14
BWQ98_05625 (PulA)		Streptococcus pneumoniae HKU1-14	AZT87326.1			14
SPOH_0989 (AmyX)		Streptococcus pneumoniae HU-OH	BBG37016.1			14
SPNHU15_01143 (PulA)		Streptococcus pneumoniae Hu15	ARD36925.1			14
SPNHU17_01144 (PulA)		Streptococcus pneumoniae Hu17	ARD34730.1			14
SPH_1211		Streptococcus pneumoniae Hungary19A-6	ACA36767.1			14
INV104_09680		Streptococcus pneumoniae INV104	CBW36651.1			14
SPNINV200_10890		Streptococcus pneumoniae INV200	CBW34722.1			14
SAMEA104035599_01034 (AmyX)		Streptococcus pneumoniae isolate b04a6400-1f66-11e7-b93e-3c4a9275d6c8	VGM80575.1			14
SAMEA2553885_01056 (AmyX)		Streptococcus pneumoniae isolate GPS_ZA_821-sc-1950967	VGM85044.1			14
SPJ_1056 (PulA)		Streptococcus pneumoniae JJA	ACO19474.1			14
KK0381_05780 (PulA)		Streptococcus pneumoniae KK0381	BAX30764.1			14
KK0981_30640 (PulA)		Streptococcus pneumoniae KK0981	BAW84556.1			14
KK1157_06110 (PulA)		Streptococcus pneumoniae KK1157	BAX32974.1			14
MDRSPN_00967 (PulI)		Streptococcus pneumoniae MDRSPN001	BBA59170.1			14
NCTC11902_01061 (Pula_2)		Streptococcus pneumoniae NCTC11902	SQG98679.1			14
NCTC12977_00588 (Pula_1)		Streptococcus pneumoniae NCTC12977	VEE27522.1			14
NCTC13276_01030 (Pula_2)		Streptococcus pneumoniae NCTC13276	SQG34290.1			14
ERS445053_01585 (Pula_2)		Streptococcus pneumoniae NCTC7465	COT21820.1			14
NCTC7466_00950 (Pula_2)		Streptococcus pneumoniae NCTC7466	SQG01993.1			14
SpnNT_01175 (AmyX)		Streptococcus pneumoniae NT_110_58	AJD72114.1			14
SPNU_1069 (AmyX)		Streptococcus pneumoniae NU83127	BBG34955.1			14
SPNOXC_10240		Streptococcus pneumoniae OXC141	CBW32680.1			14
SPP_1123 (PulA)		Streptococcus pneumoniae P1031	ACO20888.1			14
HA248_05325 (PulA)		Streptococcus pneumoniae PZ900701590	QIP99821.1			14
PulI		Streptococcus pneumoniae R6	AAK99829.1 AAT16437.1 AAW08011.1 ACK14227.1 ACW44946.1 NP_358619.1			14
E5Q10_05340 (PulA)		Streptococcus pneumoniae R6CIB17	QEL28372.1			14
HH205_04980 (PulA)		Streptococcus pneumoniae SCAID PHRX1-2019	QJI75964.1			14
BMJ42_01134 (PulA)		Streptococcus pneumoniae SP49	APJ30444.1			14
BOC71_01009 (AmyX)		Streptococcus pneumoniae SP61	APJ32513.1			14
BOC72_01009 (PulA)		Streptococcus pneumoniae SP64	APJ34538.1			14
C1H54_05315 (PulA)		Streptococcus pneumoniae SPN XDR SMC1710-32	AXJ88377.1			14
SPN034156_01120		Streptococcus pneumoniae SPN034156 SNP034156	CCP35781.1			14
SPN034183_10240		Streptococcus pneumoniae SPN034183 SNP034183	CCP32734.1			14
SPN994038_10130		Streptococcus pneumoniae SPN994038 SNP994038	CCP30749.1			14
SPN994039_10140		Streptococcus pneumoniae SPN994039 SNP994039	CCP34708.1			14
SPNA45_01410		Streptococcus pneumoniae SPNA45	CCM08695.1			14
MYY_1166		Streptococcus pneumoniae ST556	AFC94823.1			14
SPT_1164 (PulA)		Streptococcus pneumoniae Taiwan19F-14	ACO23386.1			14
HMPREF0837_11363 (PulA)		Streptococcus pneumoniae TCH8431/19A	ADI69591.1			14
SP1118		Streptococcus pneumoniae TIGR4	AAK75229.1 NP_345589.1			14
CXP32_05575 (PulA)		Streptococcus pneumoniae Xen35	AUF84890.1			14
NCTC10710_01205 (AmyX)		Streptococcus porcinus NCTC10710	VTS32251.1			14
NCTC10924_01161 (AmyX)		Streptococcus porcinus NCTC10924	VTT44558.1			14
NCTC10925_01164 (AmyX)		Streptococcus porcinus NCTC10925	VTT45903.1			14
NCTC10999_01284 (AmyX)		Streptococcus porcinus NCTC10999	SQG44563.1			14
NCTC5385_00918 (AmyX)		Streptococcus porcinus NCTC5385	VTS20458.1			14
SPPN_05555		Streptococcus pseudopneumoniae IS7493	AEL10547.1			14
NCTC13786_01976 (AmyX)		Streptococcus pseudoporcinus NCTC13786	VEF94591.1			14
FY406_00175 (PulA) (fragment)		Streptococcus ratti ATCC 31377	QEY06193.1			14
LPB404_06475 (PulA)		Streptococcus rubneri LPB0404	QXW96077.1			14
LPB404_00560 (PulA)		Streptococcus rubneri LPB0404	QXW96790.1			14
GUT183_10490 (fragment)		Streptococcus ruminantium GUT-183	BDD38811.1			14
GUT183_19180		Streptococcus ruminantium GUT-183	BDD39680.1			14
GUT184_09470 (fragment)		Streptococcus ruminantium GUT-184	BDD40683.1			14
GUT184_18460		Streptococcus ruminantium GUT-184	BDD41582.1			14
GUT189_18720		Streptococcus ruminantium GUT-189	BDD43539.1			14
GUT189_10440 (fragment)		Streptococcus ruminantium GUT-189	BDD42711.1			14
SR187_9895		Streptococcus ruminantium GUT187T	BBA93579.1			14
SR187_5610 (fragment)		Streptococcus ruminantium GUT187T	BBA92727.1			14
Ssal_00653 (fragment)		Streptococcus salivarius 57.I	AEJ53017.1			14
V471_05575 (fragment)		Streptococcus salivarius ATCC 25975	ARI59719.1			14
NX99_09630 (fragment)		Streptococcus salivarius ATCC 27945	ARI58666.1			14
SALIVB_0595 (fragment)		Streptococcus salivarius CCHSS3	CCB92898.1			14
HRE60_06745 (PulA) (fragment)		Streptococcus salivarius DB-B5	QMI51372.1			14
A6J87_08245 (fragment)		Streptococcus salivarius FDAARGOS_259	ARC49346.1			14
FOC73_02480 (PulA) (fragment)		Streptococcus salivarius FDAARGOS_771	QKH70088.1			14
HSISS4_01339 (fragment)		Streptococcus salivarius HSISS4	ALR80351.1			14
BSR14_02890 (fragment)		Streptococcus salivarius ICDC1	QGU78228.1			14
BSR19_07545 (fragment)		Streptococcus salivarius ICDC2	QGU80974.1			14
BSR20_02890 (fragment)		Streptococcus salivarius ICDC3	QGU82249.1			14
AWB63_06950 (fragment)		Streptococcus salivarius JF	AMB83148.1			14
SALIVA_1489 (fragment)		Streptococcus salivarius JIM8777	CCB95799.1			14
FHI56_09305 (PulA) (fragment)		Streptococcus salivarius LAB813	QEM33069.1			14
RDV59_08390 (PulA) (fragment)		Streptococcus salivarius LMG 11489	WMS34929.1			14
SSAL8618_07320 (fragment)		Streptococcus salivarius NCTC 8618	AIY22104.1			14
NCTC7366_00588 (Pulα-1) (fragment)		Streptococcus salivarius NCTC7366	SQF75514.1			14
NCTC8618_03909 (Pulα-1) (fragment)		Streptococcus salivarius NCTC8618	VED89554.1			14
LV497_02055 (PulA) (fragment)		Streptococcus salivarius SALI-10	UOT91017.1			14
FFV08_00635 (PulA)		Streptococcus sanguinis CGMH010	QLB51316.1			14
FFV08_04685 (PulA)		Streptococcus sanguinis CGMH010	QLB52007.1			14
FDP16_00625 (PulA)		Streptococcus sanguinis CGMH058	QLB49185.1			14
FDP16_04530 (PulA)		Streptococcus sanguinis CGMH058	QLB49859.1			14
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