Glycoside Hydrolase Family 13 / Subf 14

Activities in Familyα-amylase (EC 3.2.1.1); pullulanase (EC 3.2.1.41); cyclomaltodextrin glucanotransferase (EC 2.4.1.19); cyclomaltodextrinase (EC 3.2.1.54); trehalose-6-phosphate hydrolase (EC 3.2.1.93); oligo-α-glucosidase (EC 3.2.1.10); maltogenic amylase (EC 3.2.1.133); neopullulanase (EC 3.2.1.135); α-glucosidase (EC 3.2.1.20); maltotetraose-forming α-amylase (EC 3.2.1.60); isoamylase (EC 3.2.1.68); glucodextranase (EC 3.2.1.70); maltohexaose-forming α-amylase (EC 3.2.1.98); maltotriose-forming α-amylase (EC 3.2.1.116); branching enzyme (EC 2.4.1.18); trehalose synthase (EC 5.4.99.16); 4-α-glucanotransferase (EC 2.4.1.25); maltopentaose-forming α-amylase (EC 3.2.1.-) ; amylosucrase (EC 2.4.1.4) ; sucrose phosphorylase (EC 2.4.1.7); malto-oligosyltrehalose trehalohydrolase (EC 3.2.1.141); isomaltulose synthase (EC 5.4.99.11); malto-oligosyltrehalose synthase (EC 5.4.99.15); amylo-α-1,6-glucosidase (EC 3.2.1.33); α-1,4-glucan: phosphate α-maltosyltransferase (EC 2.4.99.16); 6'-P-sucrose phosphorylase (EC 2.4.1.-); amino acid transporter
Activities in Sub Family
Mechanism Retaining
ClanGH-H
3D Structure Status( β / α ) 8
Catalytic Nucleophile/BaseAsp (experimental)
Catalytic Proton DonorGlu (experimental)
NoteNew: many members have been assigned to subfamilies as described by Stam et al. (2006) Protein Eng Des Sel. 19, 555-562 (PMID: 17085431)
External resourcesCAZypedia; EBI Protein of the Month; HOMSTRAD; PDB Molecule of the Month; PRINTS;
Commercial Enzyme Provider(s)MEGAZYME; PROZOMIX;
Statistics GenBank accession (1483); Uniprot accession (230); PDB accession (7); 3D entries (4); cryst (1)
All (1407) Bacteria (1402) Eukaryota (1) unclassified (4) Structure (4 - 1 cryst) Characterized (33)
Bacteria
Protein Name EC#OrganismGenBank UniprotPDB/3DSubf
 pullulanase (PulAg) 3.2.1.41 Anaerobranca gottschalkii AAS47565.1 Q6QHE9   14
 pullulanase 3.2.1.41 Anaerobranca horikoshii AAP45012.1 Q6XQ00   14
 type I pullulanase (PulA) 3.2.1.41 Anoxybacillus sp. LM18-11 AEW23439.1   3WDH[A]
3WDI[A]
3WDJ[A]
14
 pullulanase (PulB) 3.2.1.41 Bacillus acidopullulyticus CAC60158.1
AAX00709.1
ACK18365.1
CAX87016.1
CAZ67879.1
CAZ67880.1
    14
 pullulanase (PulA;BaPul13A) (Pul13A) 3.2.1.41 Bacillus acidopullulyticus CAC60156.1
AAX00707.1
ACK18363.1
CAX87014.1
  2WAN[A] 14
 type II pullulanase A (PulA) 3.2.1.41 Bacillus cereus GXBC-3 AFP89962.1     14
 type I pullulanase C (PulC) 3.2.1.41 Bacillus cereus GXBC-3 AFP89964.1     14
 type I pullulanase B (PulB) 3.2.1.41 Bacillus cereus GXBC-3 AFP89963.1     14
 pullulanase 3.2.1.41 Bacillus cereus OPF-0031 AFJ04510.1     14
 amylopullulanase (PulA) (partial) 3.2.1.41 Bacillus deramificans T89.117D AAE10887.1     14
 isoamylase (Iam1) (fragment) 3.2.1.68 Bacillus lentus JNU3 AGL34022.1     14
 isoamylase (BsIam) (fragment) 3.2.1.68 Bacillus sp. CICIM 263 AGA03915.1     14
 pullulanase type I / glycogen-debranching enzyme (AmyX;BSU29930) 2.4.1.25
3.2.1.33
3.2.1.41
Bacillus subtilis subsp. subtilis str. 168 AAC00283.1
ACH14984.1
AIY94319.1
AQR83021.1
AQR87236.1
CAB14971.1
CAB14971.2
NP_390871.1
C0SPA0
O34587
2E8Y[A,B]
2E8Z[A,B]
2E9B[A,B]
14
 pullulanase (PulI;BT1663;BT_1663) 3.2.1.41 Bacteroides thetaiotaomicron VPI-5482 AAO76770.1
AAC44685.1
NP_810576.1
P71095
Q8A762
  14
 bifunctional amylopullulanase (ApuB;Bbr_0123) 3.2.1.1
3.2.1.41
Bifidobacterium breve UCC2003 AAY89038.1
ABE94835.1
F9Y041   14
 pullulanase type I (PulA) 3.2.1.41 Caldicellulosiruptor saccharolyticus AAB06264.1 Q59319   14
 pullulanase (Pul3YH5) 3.2.1.41 Exiguobacterium acetylicum a1 / YH5 AFL46505.1     14
 pullulanase (Pul-SH3) 3.2.1.41 Exiguobacterium sp. Sh3 AJG04842.1     14
 pullulanase I (PulA) 3.2.1.41 Fervidobacterium pennivorans Ven5 AAD30387.1 Q9XDB5 cryst 14
 pullulanase (GK2827) 3.2.1.41 Geobacillus kaustophilus HTA426 BAD77112.1 Q5KW24   14
 pullulanase (PulT) 3.2.1.41 Geobacillus stearothermophilus 1808262A     14
 pullulanase type I (Orf1) 3.2.1.41 Geobacillus thermoleovorans US105 CAC85704.1 Q8GJ67   14
 amylopullulanase (ApuA) 3.2.1.1
3.2.1.41
Lactobacillus plantarum L137 BAF93906.1
BAG67031.1
A9EEQ2   14
 pullulanase type I (AmyA1) 3.2.1.41 mixed culture bacterium AmyA1 AGW21593.1     14
 pulullanase type I (PulA;PbPulA) 3.2.1.41 Paenibacillus barengoltzii CAU904 AJP16551.1
WP_016313728.1
    14
 pullulanase type I (PulN) 3.2.1.41 Paenibacillus polymyxa Nws-pp2 AIE88189.1     14
 pullulanase 3.2.1.41 Pullulanibacillus naganoensis AAQ80298.1
AAQ80294.1
AAQ80299.1
AAQ80300.1
AEV53626.1
    14
 pullulanase (PulA;Tm1845) (Pul13A) 3.2.1.41 Thermotoga maritima MSB8 AAD36907.1
AAM56795.1
ABJ38302.1
ABJ38322.1
ACW20067.1
ACW20078.1
AGL50777.1
AHD18265.1
AKE29594.1
CAA04522.1
CAH68696.1
CAT81460.1
NP_229641.1
G4FGN0
O33840
  14
 pullulanase (PulA) 3.2.1.41 Thermotoga neapolitana KCCM 41025 ACN58254.1 C0LLG9   14
 pullulanase (Pul) 3.2.1.41 Thermus sp. IM6501 AAC15073.1 O69008   14
 pullulanase (Pul) 3.2.1.41 Thermus thermophilus HB8 BAB62095.1 Q7DI19   14
unclassified
Protein Name EC#OrganismGenBank UniprotPDB/3DSubf
 α-amylase 3.2.1.- uncultured organism ADD61669.1 D9ZEB5   14
 amylase 3.2.1.- uncultured organism ADD61743.1 D9ZEH9   14
Top




Last update: 2017-12-01 © Copyright 1998-2017
AFMB - CNRS - Université d'Aix-Marseille