Activities in Sub Family	[retaining] α-amylase (EC 3.2.1.1); α-glucosidase (EC 3.2.1.20); [retaining] pullulanase (EC 3.2.1.41); Isoamylase (EC 3.2.1.68);
Mechanism	Retaining a
Clan	GH-H
3D Structure Status	( β / α ) 8 barrel
Catalytic Nucleophile/Base	Asp (experimental)
Catalytic Proton Donor	Glu (experimental)
Note	New: many members have been assigned to subfamilies as described by Stam et al. (2006) Protein Eng Des Sel. 19, 555-562 (PMID: 17085431)
External resources	CAZypedia; EBI Protein of the Month; HOMSTRAD; PDB Molecule of the Month; PRINTS;
Commercial Enzyme Provider(s)	MEGAZYME; PROZOMIX;
Statistics	GenBank accession (8); Uniprot accession (39); PDB accession (25); 3D entries (6); cryst (1)

Bacteria
Protein Name	EC#	Organism	GenBank	Uniprot	PDB/3D Carbohydrate Ligands Resolution (Å)	Subf
pullulanase type I (PulA)	3.2.1.41	Anoxybacillus sp. LM18-11	AEW23439.1	K9L0H1	3WDH[A] 1.75 3WDI[A] α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp 2.20 3WDJ[A] α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp 2.22	14
pullulanase (PulA;BaPul13A) (Pul13A)	3.2.1.41	Bacillus acidopullulyticus	CAC60156.1 AAX00707.1 ACK18363.1 CAX87014.1		2WAN[A] 1.65	14
pullulanase type I / glycogen-debranching enzyme (AmyX;BSU29930)	3.2.1.41	Bacillus subtilis subsp. subtilis str. 168	AAC00283.1 ACH14984.1 AIY94319.1 AQR83021.1 AQR87236.1 CAB14971.1 CAB14971.2 NP_390871.1	A0A6M4JN50 C0SPA0 O34587	2E8Y[A,B] 2.11 2E8Z[A,B] 2.20 2E9B[A,B] α-D-Glcp-(1-4)-α-D-Glcp α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp β-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp 2.30	14
pullulanase I (PulA)	3.2.1.41	Fervidobacterium pennivorans Ven5	AAD30387.1	Q9XDB5	cryst	14
pulullanase type I (PulA;PbPulA)	3.2.1.41	Paenibacillus barengoltzii CAU904	AJP16551.1 WP_016313728.1	A0A0C5GWS2	5WVQ[A] 1.71 5WVS[A] α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp 2.03 5WVT[A] α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp 1.98 5WW1[A] 1.80 5WW2[A] α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp 1.93 5WWA[A] α-D-Glcp-(1-4)-α-D-Glcp 2.32 5WWB[A] 1.89 6JEQ[A] 1.80 6JFJ[A] α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp 1.93 6JFX[A] α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp 1.98 6JHF[A] 1.71 6JHG[A] 1.89 6JHH[A] α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp 2.03 6JHI[A] α-D-Glcp-(1-4)-α-D-Glcp 2.32	14
pullulanase (RBR_13560;Amy12)	3.2.1.41	Ruminococcus bromii L2-63	CBL15610.1	A0A2N0UU23 D4L7L5	7LSA[A] α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp 1.76 7LSR[A] α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-β-D-Glcp 2.42 7LST[A] α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-β-D-Glcp 2.05 7LSU[A] α-D-Glcp-(1-6)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-β-D-Glcp 1.95	14
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