Mechanism	Retaining a
Clan	GH-J
3D Structure Status	5-fold β-propeller
Catalytic Nucleophile/Base	Asp
Catalytic Proton Donor	Glu
Note	Subfamiles established in (Hornung and Terrapon, 2024) [PMID:37585436]
External resources	CAZypedia;
Statistics	GenBank accession (3038); Uniprot accession (63); PDB accession (44); 3D entries (12); cryst (0)

Archaea
Protein Name	EC#	Organism	GenBank	Uniprot	PDB/3D Carbohydrate Ligands Resolution (Å)	Subf
inulosucrase (InuHj; HacjB3_04715)	2.4.1.9	Halalkalicoccus jeotgali B3	ADJ14334.1	D8J9C2	7BJ4[A,B,C,D,E,F,G,H,I,J] α-D-Glcp-(1-1)-<non_carb> α-D-Glcp-(1-2)-β-D-Fruf 2.72 7BJ5[A,B,C,D,E,F,G,H,I,J] 2.75 7BJC[A,B,C,D,E,F,G,H,I,J] α-D-Glcp-(1-2)-β-D-Fruf 3.11	2
Bacteria
Protein Name	EC#	Organism	GenBank	Uniprot	PDB/3D Carbohydrate Ligands Resolution (Å)	Subf
β-fructofuranosidase (Bff;BffA;ArFFase)	3.2.1.26	Arthrobacter sp. K-1	BAB72022.1 BAB72022.2	Q8VW87	3VSR[A] 2.00 3VSS[A] β-D-Fruf 1.97 3WPU[A,B] 1.60 3WPV[A,B,C] 1.81 3WPY[A,B,C] 2.00 3WPZ[A,B,C] 2.27	2
levansucrase (SacB;BSU34450)	2.4.1.10	Bacillus subtilis subsp. subtilis str. 168	CAA26513.1 AAA22724.1 AAA22725.1 ACB45216.1 ACB46534.1 ACF93714.1 AIY94761.1 AQR83479.1 AQR87694.1 BAF93887.1 CAB08015.1 CAB15450.1 CAI39222.1 CAI39224.1 CAO78323.1 NP_391325.1	A0PFL2 P05655	1OYG[A] 1.50 1PT2[A] α-D-Glcp-(1-2)-β-D-Fruf 2.10 2VDT[A] 3.2 3BYJ[A] 2.10 3BYK[A] 2.10 3BYL[A] 2.10 3BYN[A] α-D-Galp-(1-6)-α-D-Glcp-(1-2)-β-D-Fruf 2.10 6PWQ[A,B] 2.60 6VHQ[A,B] β-D-6-deoxy-Fruf-(2-6)-β-D-Fruf β-D-Fruf-(2-6)-β-D-Fruf-(2-6)-β-D-Fruf-(2-6)-β-D-Fruf-(2-6)-β-D-Fruf-(2-6)-β-D-Fruf 2.05	1
β-fructosyltransferase (BiBftA; Bind_2021)	2.4.1.10	Beijerinckia indica subsp. indica ATCC 9039	ACB95643.1	B2IF78	6M0D[A] 2.20 6M0E[A] β-D-Fruf β-D-Frup 1.35 8I2Q[A] 2.15 8I2R[A] β-D-Fruf β-D-Frup 1.36	2
levansucrase (Brs-LS; EH206_19850)	2.4.1.10	Brenneria nigrifluens ATCC 13028	QCR06203.1	A0A2U1UP91 G7LSK3	7EHR[A] 1.33 7EHS[A] 1.60 7EHT[A] 1.45 7FDZ[A] α-D-Glcp-(1-2)-β-D-Fruf 1.35	2
levansucrase (LsC; EAM_3468)	2.4.1.10	Erwinia amylovora ATCC 49946	CBJ48143.1	A0A0M3KKU6 D4IGH9	4D47[A,B,C,D,E,F,G,H] α-D-Glcp β-D-Fruf 2.77	2
levansucrase (LsC; EtLsc; ETA_34670)	2.4.1.10	Erwinia tasmaniensis Et1/99	CAO98513.1	B2VCC3	6FRW[A] 1.52 6RV5[A] β-D-Fruf-(2-6)-β-D-Fruf 1.58 7OSO[A] 1.40	2
levansucrase (LdsA)	2.4.1.10	Gluconacetobacter diazotrophicus SRT4	AAB36606.1	Q43998 Q5I5I3	1W18[A,B] 2.5	2
inulosucrase (InuJ;LJ0913)	2.4.1.9	Lactobacillus johnsonii NCC 533	AAS08734.1 NP_964768.1	Q74K42	2YFR[A] 1.75 2YFS[A] α-D-Glcp-(1-2)-β-D-Fruf 2.60 2YFT[A] α-D-Glcp-(1-2)-β-D-Fruf-(1-2)-β-D-Fruf 1.85	1
inulosucrase (Inu;FtfA)	2.4.1.9	Limosilactobacillus reuteri 121	AAN05575.1 AAR67548.1 AAU98704.1	A0A7X2FZN4 Q8GP32	8XVP[A,B] 1.98 8XVQ[A,B] β-D-Fruf 2.14 8XVR[A,B,C,D] 2.44	1
levansucrase (SacB; BMD_1536; LS19)	2.4.1.10	Priestia megaterium DSM 319	ADF38395.1	D5DC07	3OM2[A] 1.90 3OM4[A,B,C,D] 1.75 3OM5[A,B,C,D] 1.95 3OM6[A,B,C,D] 1.96 3OM7[A,B,C,D] 1.86	1
KPSA3_102921		Pseudomonas syringae pv. actinidiae MAFF212211	BBM09195.1		8QJ5[A,B] 1.63 8QKW[A] 1.65	2
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