Glycoside Hydrolase Family 5 / Subf 8

Mechanism	Retaining e
Clan	GH-A
3D Structure Status	( β / α ) ₈ barrel
Catalytic Nucleophile/Base	Glu (experimental)
Catalytic Proton Donor	Glu (experimental)
Note	Once known as cellulase family A; many members have been assigned to subfamilies as described by Aspeborg et al. (2012) BMC Evol Biol. 12(1):186 (PMID: 22992189).
External resources	CAZypedia; HOMSTRAD; PROSITE;
Commercial Enzyme Provider(s)	MEGAZYME; NZYTech; PROZOMIX;
Statistics	GenBank accession (10); Uniprot accession (38); PDB accession (10); 3D entries (6); cryst (0)

Summary

Download GH5_8 (1463) Taxonomic display Structure (6) Characterized (39) Activities in Sub Family (1)

Bacteria
Protein Name	EC#	Reference	Organism	GenBank	Uniprot	PDB/3D	Subf
β-mannanase (Amn5) (Man5A)	3.2.1.78	doi	Bacillus sp. JAMB-602	BAD99527.1	Q4W8M3	1WKY[A]	8
β-mannanase (ManA; MAN330) (Man5A)	3.2.1.78	pubmed	Bacillus sp. N16-5	AAT06599.1 AML27065.1	Q5YEX6	3JUG[A]	8
β-1,4-mannanase (BlMan5_8; Balac_1450)	3.2.1.78	pubmed	Bifidobacterium animalis subsp. lactis Bl-04	ACS46797.1	C6A9J9		8
β-1,4-mannanase (ManA)	3.2.1.78	pubmed	Caldibacillus cellulovorans	AAF22274.1	Q9RFX5		8
bifunctional mannanase / endoglucanase (CelB; CbMan5A/Cel44A; Athe_1859; Cbes_1859; ORF1946) (Man5A-Cel44A)	3.2.1.78	pubmed	Caldicellulosiruptor bescii DSM 6725	ACM60947.1	B9MKT9		8
bifunctional cellulase / β-mannanase (CelE; CelC-ManB; CbCel9B/Man5A; Athe_1865; Cbes_1865; Orf1952) (Cel9B-Man5A)	3.2.1.78	pubmed	Caldicellulosiruptor bescii DSM 6725	ACM60953.1	B9MKU5		8
multifunctional glycoside hydrolase (CelD; CelB; CbMan5C/Cel5A; Athe_1866) (Man5C-Cel5A)	3.2.1.78	pubmed	Caldicellulosiruptor bescii DSM 6725	ACM60954.1			8
multifunctional glycoside hydrolase (Cmor_0543; OTK00_002183)	3.2.1.78	doi	Caldicellulosiruptor morganii DSM 8990	WAM33661.1 WP_082054593.1			8
bifunctional β-mannanase / endo-β-1,4-glucanase (ManA)	3.2.1.78	pubmed	Caldicellulosiruptor saccharolyticus	AAA71887.1 AAA72861.1	P22533		8
endo-β-1,4-mannanase (ManB)	3.2.1.78	doi	Cellulosimicrobium cellulans YB-43	AQQ16388.1			8
β-1,4-mannanase (ManH)	3.2.1.78	pubmed	Cellulosimicrobium sp. HY-13	AEE43708.1	G3FBL1		8
endo-β-1,4-mannanase 5A (CjMan5A; CJA_3338) (Man5A)	3.2.1.78	pubmed	Cellvibrio japonicus Ueda107	ACE84673.1 AAO31759.1	B3PF24 Q840C2		8
endo-β-1,4-mannanase 5B (CJA_2475) (Man5B)	3.2.1.78	pubmed	Cellvibrio japonicus Ueda107	AAO31760.1 ACE84941.1	B3PKK7 Q840C1		8
β-mannanase (EsGH5_8; EUS_11000)	3.2.1.78	pubmed	[Eubacterium] siraeum 70/3	CBK96294.1	D4JT75		8
β-mannanase (TM1)	3.2.1.78	pubmed	Niallia circulans	AAT39478.1	Q6IUP2		8
β-mannanase (Man1;ManB48)	3.2.1.78	pubmed	Niallia circulans CGMCC 1416	AAX87002.1	Q49HI4		8
β-mannanase (Man5A)	3.2.1.78	pubmed	Niallia circulans CGMCC1554	AAX87003.1	Q0IJR6		8
β-mannanase (G)	3.2.1.78	pubmed	Niallia circulans K-1	BAA25878.1	O66185		8
β-mannanase (MANPN11) (fragment)	3.2.1.78	doi	Niallia nealsonii PN-11	AGU71466.2 AGU71466.1 AGU71466.3	T1Z249		8
β-1,4-mannanase (AmnA)	3.2.1.78	doi	Pseudomonas sp. ND137	BAB79290.2	Q8RR71		8
β-mannanase (GH5C;RUM_21650) (Man5A)	3.2.1.78	pubmed	Ruminococcus champanellensis 18P13 = JCM 17042	CBL18180.1	D4LEY5		8
β-mannanase (Sde_0656) (Man5O)	3.2.1.78	pubmed	Saccharophagus degradans 2-40	ABD79918.1	Q21N11		8
β-mannanase 5A (BaMan5A) (Man5A)	3.2.1.78	pubmed	Salipaludibacillus agaradhaerens	AAN27517.1 AAN27518.1 AAQ31837.1 AAQ79152.1 AAQ79153.1 ABC13790.1 ABC13791.1	G1K3N4 Q5YEX6	2WHJ[A] 2WHL[A]	8
β-mannanase (ManA; SlMan)	3.2.1.78	pubmed	Streptomyces lividans 1326	AAA26710.2	P51529		8
β-1,4-mannanase (Man5S27)	3.2.1.78	pubmed	Streptomyces sp. S27	ADK91085.1	E2F4D4		8
β-mannanase (SACTE_2347)	3.2.1.78	pubmed	Streptomyces sp. SirexAA-E	AEN10237.1 WP_014046222.1	A0A327SUT4 G2NHM6	4FK9[A]	8
β-mannanase (Man;StMan)	3.2.1.78	pubmed	Streptomyces thermolilacinus NBRC14274	BAK26781.1	F5HR99	3WSU[A,B] 4Y7E[A,B]	8
β-mannanase (ManII; StManII)	3.2.1.78	pubmed	Streptomyces thermoluteus NBRC 14269	BAM62868.1	K4PWK0		8
endomannanase (Man5ATc) (Man5A)	3.2.1.78	pubmed	Thermobifida cellulosilytica TB100	AHB89703.1	A0A0A7A855		8
β-mannanase (Tfm)	3.2.1.78	pubmed	Thermobifida fusca BCRC1914	AID15578.1	A0A068CD09		8
β-mannanase Q1.1 (Man) (partial)	3.2.1.78	pubmed	Thermobifida fusca KW3	CAA06924.1	Q9ZF13	2MAN[A] 3MAN[A] 1BQC[A]	8
endomannanase (Man5ATf; TM51_04883) (Man5A)	3.2.1.78	pubmed	Thermobifida fusca TM51	AHB89702.1	R9FGU8		8
β-mannanase (Man; TfMan; Tfu-man; Tfu_0900)	3.2.1.78	pubmed	Thermobifida fusca YX	AAZ54938.1	Q47RH9		8
endomannanase (Man5ATh) (Man5A)	3.2.1.78	pubmed	Thermobifida halotolerans YIM90462	AHB89704.1	A0A0A7A809		8
β-mannanase (ManA)	3.2.1.78	doi	Vibrio sp. MA-138	BAA25188.1	O69347		8
endo-β-1,4-mannosidase (ManA;XCC1778)	3.2.1.78	pubmed	Xanthomonas campestris pv. campestris str. ATCC 33913	AAM41068.1 NP_637144.1	Q8P9S0		8
β-mannanase (XcGH5_8; TP37_09655)	3.2.1.78	pubmed	Xanthomonas citri pv. aurantifolii FDC 1559	AMU98328.1			8
Eukaryota
Protein Name	EC#	Reference	Organism	GenBank	Uniprot	PDB/3D	Subf
β-mannanase (Man1;HhMAN1)	3.2.1.78	pubmed	Hypothenemus hampei	ACU52526.1 ACU52527.1 ADF22325.1	C8CAP6		8
endo-β-1,4-mannanase (LpsGH5_8)	3.2.1.78	pubmed	Lyrodus pedicellatus	VAX65844.1	A0A8K1XLY2		8
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