Mechanism	Retaining e
Clan	GH-A
3D Structure Status	( β / α ) 8 barrel
Catalytic Nucleophile/Base	Glu (experimental)
Catalytic Proton Donor	Glu (experimental)
Note	Once known as cellulase family A; many members have been assigned to subfamilies as described by Aspeborg et al. (2012) BMC Evol Biol. 12(1):186 (PMID: 22992189).
External resources	CAZypedia; PROSITE;
Statistics	GenBank accession (12); Uniprot accession (158); PDB accession (48); 3D entries (18); cryst (0)

Bacteria
Protein Name	EC#	Organism	GenBank	Uniprot	PDB/3D Carbohydrate Ligands Resolution (Å)	Subf
cellulose endo-β-1,4-glucosidase 5A (AaCel5A) (Cel5A)	3.2.1.4	Acetivibrio alkalicellulosi	TYQ14625.1		8ZVK[A] 2.00	2
cellulase (BCE103)		Bacillus sp. (in: firmicutes) CBS 670.93	CAB59165.1 AAV25061.1 CAB58698.1 CAD82873.1		1LF1[A] 1.70	2
endo-β-1,4-glucanase K (Egl-K)	3.2.1.4	Bacillus sp. KSM-635	AAA22304.1	P19424	1G01[A] 1.90 1G0C[A] β-D-Glcp-(1-4)-α-D-Glcp 1.90	2
endo-β-1,4-glucanase (EglS; BglC; Gld; BsCel5; BsCel5A; BSU18130) (Cel5A)	3.2.1.4	Bacillus subtilis subsp. subtilis str. 168	CAA82317.1 AEJ76923.1 AIY93123.1 AQR81804.1 AQR86018.1 CAA97610.1 CAB13696.2 NP_389695.1 NP_389695.2	A0A6M3ZBL5 G1DE47 P10475	3PZT[A,B] 1.97 3PZU[A,B] 2.10 3PZV[A,B,C,D] 2.87	2
endoglucanase (Csac_0678)	3.2.1.4	Caldicellulosiruptor saccharolyticus DSM 8903	ABP66297.1	A4XHB2	5ECU[A] 1.50	2
endo-β-1,4-glucanase E / 5B (CelE;CJA_2983) (Cel5B)	3.2.1.4	Cellvibrio japonicus Ueda107	ACE84076.1 CAA60493.1	B3PCS3 Q59665	8BQA[A] 8BQC[A] 1.57	2
periplasmic endoglucanase 5B (CHU_2103) (Cel5B)	3.2.1.4	Cytophaga hutchinsonii ATCC 33406	ABG59366.1	A0A6N4ST27 Q11T98	5IHS[A] 1.10	2
endo-β-1,4-glucanase Z (CelZ; EgZ; Dda3937_02793) (Cel5A=Cel5Z)	3.2.1.4	Dickeya dadantii 3937	ADM99099.1 CAA68604.1	P07103	1EGZ[A,B,C] 2.30	2
endo-β-1,4-glucanase (CelA; GsCelA)	3.2.1.4	Geobacillus sp. 70PC53	ACJ60856.1	C5H6X3	4XZB[A] 1.62 4XZW[A] 1.50	2
endoglucanase (CelG) (Cel5G)	3.2.1.4	Pseudoalteromonas haloplanktis A23	CAA76775.1	O86099	1TVN[A,B] 1.41 1TVP[A,B] β-D-Glcp-(1-4)-β-D-Glcp 1.60	2
endo-β-1,4-glucanase 5A (Cel5A)	3.2.1.4	Salipaludibacillus agaradhaerens	AAC19169.1	O85465	1A3H[A] 1.57 1E5J[A] β-D-Glcp-(1-4)-α-D-Glcp4S-(1-4)-β-D-Glcp4S-(1-4)-β-D-Glcp-(1-1)-methyl 1.85 1H11[A] β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-α-D-Glcp2F-(1-4)-GLU 1.08 1H2J[A] β-D-Glcp-(1-4)-α-D-Glcp2F-(1-4)-GLU 1.15 1H5V[A] β-D-Glcp-(1-4)-β-D-Glcp4S-(1-4)-β-D-Glcp4S-(1-4)-β-D-Glcp4S-(1-4)-α-D-Glcp4S-(1-1)-methyl 1.1 1HF5[A] β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-α-D-Glcp2F-(1-4)-GLU 1.10 1HF6[A] β-D-Glcp-(1-4)-β-D-Glcp-(1-1)-<non_carb> 1.15 1HF7[A] β-D-Glcp-(1-4)-α-D-Glcp2F-(1-4)-GLU β-D-Glcp-(1-4)-β-D-Glcp2F-(1-1)-<non_carb> 1.10 1OCQ[A] β-D-Glcp-(1-4)-<carb_like_IFM> <carb_like_IFM> 1.08 1QHZ[A] 1.95 1QI0[A] β-D-Glcp-(1-4)-β-D-Glcp 2.10 1QI2[A] β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-α-D-Glcp2F-(1-4)-GLU 1.75 1W3K[A] β-D-Glcp-(1-1)-<non_carb> <carb_like_OXZ> 1.2 1W3L[A] β-D-Glcp-(1-4)-β-D-Glcp-(1-1)-<non_carb> <carb_like_OXZ> 1.04 2A3H[A] β-D-Glcp-(1-4)-β-D-Glcp 2.00 2V38[A] β-D-Glcp-(1-1)-<non_carb> <carb_like_IFL> 1.50 3A3H[A] β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-α-D-Glcp 1.64 4A3H[A] β-D-Glcp-(1-4)-β-D-Glcp2F-(1-1)-<non_carb> 1.65 5A3H[A] β-D-Glcp-(1-4)-α-D-Glcp2F-(1-4)-GLU 1.82 6A3H[A] β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-α-D-Glcp2F-(1-4)-GLU 1.68 7A3H[A] 0.95 8A3H[A] β-D-Glcp-(1-1)-<non_carb> 0.97	2
endo-β-1,4-glucosidase (TwCel5-6)	3.2.1.73	Teredinibacter sp. 82 / Teredinibacter waterburyi	WAK85940.1	A0A9E9C620 A0AA82WPD7	8C10[A] 1.00	2
endo-β-1,4-glucanase / celulase (CelDZ1a)	3.2.1.4	Thermoanaerobacterium sp. A57Txylan	ALX38276.1	A0A0U4EBH5 I3VS73	5FIP[A,B,C,D] 1.88	2
endo-β-1,4-glucanase 5A (E5;CelE;TfCel5A;Tfu_0901) (Cel5A)	3.2.1.4	Thermobifida fusca YX	AAZ54939.1 AAC09379.1	Q01786 Q47RH8	2CKR[A,B] β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp 1.77 2CKS[A,B] 1.6	2
cellulase 5R (Cel5R;Cel5R_FS)	3.2.1.4	uncultured bacterium	AND74761.1	A0A172PZJ4	5I2U[A,B] 2.20	2
cellulase (CelE1)	3.2.1.4	uncultured bacterium	AHA42547.2 AHA42547.1	A0A0R5P8X1 V5KYY9	4M1R[A,B] 1.80	2
unclassified
Protein Name	EC#	Organism	GenBank	Uniprot	PDB/3D Carbohydrate Ligands Resolution (Å)	Subf
ancestral endocellulase Cel5A (LFCA_EG)	3.2.1.4	synthetic construct			6GJF[B,C,D,E,F] 1.45 6GJF[A] 1.45	2
SARM_0026 / 457020_01700 / TW-40	3.2.1.4	uncultured organism	ADX05697.1	E9NSK2	5UHX[A] 1.57 5WH8[A] 1.57	2
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