Mechanism	Retaining a
Clan	GH-T
3D Structure Status	( β / α ) 7 barrel
Catalytic Nucleophile/Base	Glu
Catalytic Proton Donor	Asp
Note	Subfamilies established in (Polacek et al., 2025) [PMID:40187459]
External resources	CAZypedia;
Statistics	GenBank accession (851); Uniprot accession (12); PDB accession (20); 3D entries (1); cryst (0)

Bacteria
Protein Name	EC#	Organism	GenBank	Uniprot	PDB/3D Carbohydrate Ligands Resolution (Å)	Subf
amylopullulanase (AaApu; APU_Aquae; Aq_720)	3.2.1.1 3.2.1.41	Aquifex aeolicus VF5	AAC06900.1 NP_213494.1 WP_010880432.1	O66934	8ZYI[A,B] α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-6)-α-D-Glcp-(1-4)-α-D-Glcp 1.66 9IHT[A,B] <non_carb>-(1-4)-α-D-6-deoxy-Glcp4N-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp 1.69 9IHU[A,B] 1.79 9IHV[A,B] α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp 1.78 9IHW[A,B] α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp 1.64 9IHX[A,B] α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp 1.86 9II0[A,B] α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp 1.89 9II1[A,B] α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-β-D-Glcp 1.50 9JLQ[A,B,C,D] 2.54 9JLR[A,B,C,D] α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp 2.79 9JLS[A,B,C,D] <non_carb>-(1-4)-α-D-6-deoxy-Glcp4N-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp 3.06 9JLT[A,B] 1.56 9JLU[A] 1.88 9JLV[A] α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp 1.51 9JLW[A,B] α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp 1.95 9K7C[A,B,C,D] α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp 1.85 9K7D[A,B] α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp 1.72 9KYP[A,B,C,D] 1.79 9KYQ[A,B] α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp 1.60 9KYR[A,B,C,D] 1.89	2
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