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Glycoside Hydrolase Family 31

Mechanism Retaining a
ClanGH-D
3D Structure Status( β / α ) 8 barrel
Catalytic Nucleophile/BaseAsp
Catalytic Proton DonorAsp
NoteSubfamily classification initiated by Arumapperuma et al. 2023 [PMID:36806678]
External resourcesCAZypedia; PROSITE;
Commercial Enzyme Provider(s)MEGAZYME;
Statistics GenBank accession (28578); Uniprot accession (179); PDB accession (165); 3D entries (37); cryst (0)
Summary
Download GH31 (34710) Taxonomic display Structure (37) Characterized (138) Activities in Family (17)
Archaea
Protein Name EC#OrganismGenBankUniprot
PDB/3D Carbohydrate Ligands Resolution (Å)
 Subf
 α-glucosidase with α-mannosidase activity (MalA;SsMalA;Sso1_0793) 3.2.1.20
3.2.1.24		 Saccharolobus solfataricus 98/2 AAC38215.1
ACX91047.1
AET42970.1		 D0KQM8
G8GCW5
O59645
P0CD66
2G3M[A,B,C,D,E,F] 2.55
2G3N[A,B,C,D,E,F] β-D-Glcp-(1-1)-ethyl
 2.55
1
Bacteria
Protein Name EC#OrganismGenBankUniprot
PDB/3D Carbohydrate Ligands Resolution (Å)
 Subf
 EUBREC_2592   Agathobacter rectalis ATCC 33656 ACR76323.1  
6PNR[A,C] 1.90
13
 sulfoquinovosidase (SY94_3281) 3.2.1.199 Agrobacterium tumefaciens F4 KJX86999.1 A0A083ZKV2
A9CEZ0
5OHS[A,B,C,D,E,F,G,H] α-D-Glcp6SH-(1-1)-<non_carb>
 1.97
5OHY[A,B,C,D] 1.77
7OFX[A,B,C,D] α-D-Glcp6SH-(1-1)-<non_carb>
 2.15
13
 α-xylosidase (Yici_5;BoGH31A;BACOVA_02646;Bovatus_03066) 3.2.1.177 Bacteroides ovatus ATCC 8483 ALJ47674.1 A7LXT0
5JOU[A] 1.50
5JOV[A] α-L-Idop5F-(1-4)-ASP
 1.50
4
 exo-α-1,4-galactosidase (BsGH31_19; K6V19_13215) 3.2.1.- Bacteroides salyersiae BFG-288 UBD15097.1 A0A7J4XIY8
8J53[A,B,C,D,E] 3.50
19
 α-glucosidase (BT0339;BT_0339) 3.2.1.20 Bacteroides thetaiotaomicron VPI-5482 AAO75446.1
NP_809252.1		 Q8AAX3
5F7C[A,B,C,D] 2.60
3
 α-glucosidase (BT3299;BT_3299) 3.2.1.20 Bacteroides thetaiotaomicron VPI-5482 AAO78405.1
NP_812211.1		 Q8A2K6
5DJW[A,B] 2.70
1
 α-glucosidase / maltase-glucoamylase (Ro-aG1;RUMOBE_03919) 3.2.1.20 Blautia obeum ATCC 29174 ZP_01966167.1 A5ZY13
3FFJ[A,B] 1.94
3M46[A,B] 2.66
3M6D[A,B] 2.90
3MKK[A,B] α-D-Glcp-(1-6)-β-D-Glcp
 1.91
3N04[A,B] 2.02
3NQQ[A,B] α-D-Glcp
<non_carb>-(1-4)-α-D-6-deoxy-Glcp4N-(1-4)-α-D-Glcp-(1-4)-α-D-Manp
 1.99
3NSX[A,B] 1.57
3NUK[A,B] 2.06
3NXM[A,B,C,D] <non_carb>-(1-4)-α-D-6-deoxy-Glcp4N-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp
 2.17
3PHA[A,B,C,D] <non_carb>-(1-4)-α-D-6-deoxy-Glcp4N-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp
 2.17
3POC[A,B] α-D-Glcp
<non_carb>-(1-4)-α-D-6-deoxy-Glcp4N-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp
 1.99
6C9X[A,B] 1.46
6C9Z[A,B] 2.10
6CA1[A,B] <carb_like_MIG>
 1.95
6CA3[A,B] <carb_like_MIG>
 1.74
1
 oligosaccharide α-1,4-transglucosylase 31B (CjAgd31B; CJA_3248) (Agd31B) 2.4.1.161 Cellvibrio japonicus Ueda107 ACE84782.1 B3PEE6
4B9Y[A] 1.90
4B9Z[A] <non_carb>-(1-4)-α-D-6-deoxy-Glcp4N-(1-4)-β-D-Glcp-(1-4)-β-D-Gulp
 2.00
4BA0[A] β-D-Glcp5F-(1-4)-ASP
 1.85
5I23[A] 1.95
5I24[A] 1.85
5NPB[A] 1.90
5NPC[A] 1.96
5NPD[A] 1.95
5NPE[A] 1.95
7P4C[A]
7P4D[A]
8RW3[A,B,C] 1.90
 α-xylosidase 31A (CjXyl31A;CJA_2706) (Xyl31A) 3.2.1.177 Cellvibrio japonicus Ueda107 ACE86259.1 B3PBD9
2XVG[A] 2.60
2XVK[A] β-D-Xylp5F-(1-4)-ASP
 2.50
2XVL[A] 2.30
4
 α-N-acetylgalactosaminidase (EfGH31; WOW_01747) 3.2.1.217 Enterococcus faecalis ATCC 10100 EOK08638.1 A0A125W693
Q833V2
6M76[A] 1.40
6M77[A] β-D-GalpNAc
 1.90
7F7Q[A] α-D-GalpNAc-(1-1)-<non_carb>
 1.42
7F7R[A] 1.63
18
 α-xylosidase (YicI;EcYicI;b3656) 3.2.1.177 Escherichia coli str. K-12 substr. MG1655 AAC76680.1
AAA62009.1
AIZ93543.1
ALI38655.1
AMC96544.1
AUG18368.1
AVI53824.1
AYG17401.1
NP_418113.1		 P31434
1WE5[A,B,C,D,E,F] 2.40
1XSI[A,B,C,D,E,F] 2.20
1XSJ[A,B,C,D,E,F] 2.10
1XSK[A,B,C,D,E,F] β-D-Xylp5F-(1-4)-ASP
 2.20
2F2H[A,B,C,D,E,F] α-D-Xylp-(1-6)-β-D-Glcp6SH-(1-1)-<non_carb>
 1.95
3
 sulfoquinovosidase (SQase;YihQ;b3878) 3.2.1.199 Escherichia coli str. K-12 substr. MG1655 AAC76875.1
AAB03011.1
AIZ93343.1
ALI38454.1
AMC96746.1
AUG18567.1
AVI54027.1
AYG21326.1
NP_418314.1		 P32138
5AED[A,B] 1.91
5AEE[A,B] α-D-Glcp6SH-(1-1)-<non_carb>
 1.85
5AEG[A,B] α-L-Idop5F-(1-4)-ASP
 1.85
5OHT[A,B] 1.87
13
 exo-α-1,4-galactosidase (FpGH31_19) 3.2.1.- Flavihumibacter petaseus WP_072053931.1 A0A0E9MUN5
8J50[A,B] 1.90
8J51[A,B] α-D-Galp
 2.15
8J52[A,B] β-D-Galp
D-1-deoxy-Galp
 1.90
19
 dextranase / 6-α-glucosyltransferase (FjDex31A; Fjoh_4430) 3.2.1.11
2.4.1.24		 Flavobacterium johnsoniae UW101 ABQ07434.1 A5FBI1
6JR6[A,B,C,D] 2.00
6JR7[A,B,C,D] α-D-Glcp-(1-6)-α-D-Glcp
 1.75
6JR8[A,B,C,D] α-D-Glcp-(1-6)-α-D-Glcp-(1-6)-α-D-Glcp
 1.80
 AM334_05995   Klebsiella aerogenes AR_0007 ATY00397.1  
8T7Z[A,B,C,D,E,F] 2.70
3
 cycloalternan-specific α-1,3-glucosidase / α-1,3-isomaltosidase (Kfla_1895) 3.2.1.204 Kribbella flavida DSM 17836 ADB30989.1 D2PPM7
5X3I[A,B] 2.10
5X3J[A,B] 2.30
5X3K[A,B] α-D-Glcp-(1-6)-α-D-Glcp
 2.50
7
 α-glucosidase with preference for α-1,3-glucosidic bonds (LlGH31_u1; lmg_1836) 3.2.1.20
3.2.1.84		 Lactococcus cremoris subsp. cremoris MG1363 CAL98407.1 A2RM80
7WJ9[A,B,C,D,E,F] 1.75
7WJA[A] 1.85
7WJB[A] α-D-Glcp
 2.00
7WJC[A] α-D-Glcp-(1-3)-α-D-Glcp
 1.75
7WJD[A] α-D-Glcp-(1-3)-α-D-Glcp-(1-3)-α-D-Glcp
 1.80
7WJE[A] α-D-Glcp-(1-3)-α-D-Glcp-(1-3)-α-D-Glcp-(1-3)-α-D-Glcp
 1.80
7WJF[A] α-D-Glcp-(1-2)-α-D-Glcp
 1.80
7WLG[A,B,C,D,E,F] 2.73
15
 cycloalternan-forming enzyme / 3-α-isomaltosyltransferase (CAFE;LmCAFE;Lmo2446) 2.4.1.387 Listeria monocytogenes EGD-e CAD00524.1
NP_465969.1		 Q8Y4J2
4KMQ[A] 1.90
4KWU[A] α-D-Glcp
β-D-Glcp
 1.90
5F7U[A] α-D-Glcp-(1-4)-α-D-Glcp
α-D-Glcp-(1-6)-α-D-Glcp
α-D-Glcp-(1-6)-α-D-Glcp-(1-3)[α-D-Glcp-(1-6)]-α-D-Glcp
α-D-Glcp-(1-6)-α-D-Glcp-(1-4)-α-D-Glcp
 1.70
5HPO[A] α-D-Glcp-(1-4)-α-D-Glcp
α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp
 1.80
5HXM[A] α-D-Glcp-(1-6)-α-D-Glcp
 1.90
5I0D[A,B] α-D-Glcp
α-D-Glcp-(1-6)-α-D-Glcp
β-D-Manp
 1.77
7
 6-α-glucosyltransferase (Ps6GT31A) (6GT31A) 2.4.1.24 Paenibacillus sp. 598K BAV17026.1 A0A193PKW5
5X7O[A,B] 2.00
5X7P[A,B] α-D-6-deoxy-Glcp4N-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp
α-D-Glcp-(1-4)-α-D-Glcp
<non_carb>-(1-4)-α-D-6-deoxy-Glcp4N
<non_carb>-(1-4)-α-D-6-deoxy-Glcp4N-(1-4)-α-D-Glcp
<non_carb>-(1-4)-α-D-6-deoxy-Glcp4N-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp
 2.40
5X7Q[A,B] α-D-Glcp
α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp
α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp
α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp
α-D-Glcp-(1-6)-α-D-Glcp
α-D-Glcp-(1-6)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-β-D-Glcp
β-D-Glcp
<non_carb>-(1-4)-α-D-6-deoxy-Glcp4N
 1.95
5X7R[A,B] α-D-Glcp
α-D-Glcp-(1-4)-α-D-Glcp
α-D-Glcp-(1-6)-α-D-Glcp-(1-6)-α-D-Glcp
α-D-Glcp-(1-6)-α-D-Glcp-(1-6)-α-D-Glcp-(1-6)-α-D-Glcp-(1-6)-β-D-Glcp
β-D-Glcp
<non_carb>-(1-4)-α-D-6-deoxy-Glcp4N
 1.95
5X7S[A,B] 2.40
10
 a-(6-sulfo)-quinovosidase (BMQ_3646) 3.2.1.199 Priestia megaterium QM B1551 ADE70659.1 D5E1T0
8R56[A,B,C,D] 2.45
13
 α-galactosidase (PsGal31A) (Gal31A) 3.2.1.22 Pseudopedobacter saltans NBRC 100064 BAR72452.1 A0A0F7R6D6
4XPO[A] 2.10
4XPP[A] β-D-Galp
 2.30
4XPQ[A] β-L-Fucp
 1.85
4XPR[A] 2.01
4XPS[A] α-D-Galp-(1-1)-<non_carb>
 2.10
14
 cycloalternan-specific α-1,3-glucosidase (CADE;TpCADE;TpLmo0182;CQ11_05330) 3.2.1.204 Trueperella pyogenes TP6375 AHU89502.1 A0A806M5Y7
5F7S[A,B] 2.30
5I0E[B] α-D-Glcp-(1-6)-α-D-Glcp
 2.30
5I0F[B] α-D-Glcp-(1-6)-α-D-Glcp
α-D-Glcp-(1-6)-β-D-Glcp-(1-4)-ASP
 1.85
5I0G[B] 2.15
7
 α-xylosidase (XacXyl31; XylS; XAC1773) 3.2.1.177 Xanthomonas citri pv. citri str. 306 AAM36637.1
NP_642101.1
WP_011051131.1		 A0A7S6Z021
A0A8I0H821
M4TT53
Q8PLM2
7KMP[A] 1.56
7KNC[A] α-D-Xylp
 1.87
4
Eukaryota
Protein Name EC#OrganismGenBankUniprot
PDB/3D Carbohydrate Ligands Resolution (Å)
 Subf
 α-xylosidase (AX; AxlA; Axl1; Aspni5|43342) 3.2.1.177 Aspergillus niger ATCC 1015 DAA35002.1 A2QTU5
G3XMN9
6DRU[A,B] α-D-Xylp
α-D-Xylp-(1-6)[β-D-Glcp-(1-4)]-β-D-Glcp-(1-4)-β-D-Glcp
β-D-Galp-(1-2)-α-D-Xylp-(1-6)[β-D-Glcp-(1-4)]-β-D-Glcp-(1-4)-β-D-Glcp
 2.70
5
 α-glucosidase (AgdL; AsojAgdL) 3.2.1.20 Aspergillus sojae NBRC 4239   A0A5N6DPG0
A0A5N6EXS6
7XOI[A,C,E,G,I,K,M,O,B,D, α-D-Glcp
α-D-Glcp-(1-1)-α-D-Glcp
 2.30
1
 α-glucosidase (Agl;SBG) 3.2.1.20 Beta vulgaris BAM74081.1
BAM72724.1		 L0N1H0
L0N7E5
3W37[A] <non_carb>-(1-4)-α-D-6-deoxy-Glcp4N-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp
 1.70
3W38[A] 2.79
3WEL[A] <non_carb>-(1-4)-α-D-6-deoxy-Glcp4N-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp
 1.84
3WEM[A] <non_carb>-(1-4)-α-D-6-deoxy-Glcp4N-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp
 2.59
3WEN[A] <non_carb>-(1-4)-α-D-6-deoxy-Glcp4N-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp
 2.59
3WEO[A] <non_carb>-(1-4)-α-D-6-deoxy-Glcp4N-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp
 1.45
1
 α-1,4-glucan lyase isozyme 1 (GLq1;Ag111) 4.2.2.13 Gracilariopsis lemaneiformis CAB51910.1
AAR66623.1
ABA16064.1		 Q9STC1
2X2H[A,B,C,D] 2.06
2X2I[A,B,C,D] <non_carb>-(1-4)-α-D-6-deoxy-Glcp4N-(1-4)-α-D-Glcp
<non_carb>-(1-4)-α-D-6-deoxy-Glcp4N-(1-4)-α-D-Glcp-(1-4)-β-D-Glcp
 2.60
2X2J[A,B,C,D] <carb_like_NOJ>
 2.35
4AMW[A,B,C,D] α-L-Idop5F-(1-4)-ASP
 1.90
4AMX[A,B,C,D] β-D-Glcp5F-(1-4)-ASP
 2.10
11
 α-glucosidase A (GAA) (lysosomal) 3.2.1.20 Homo sapiens CAA68763.1
AAA52506.1
AAB33842.1
AAH40431.1
ABI53718.1
ACM80396.1
ACM84029.1
BAH13042.1
CAA38907.1
CAA68764.1
CAC12967.1
CAE85448.1
NP_000143.1
SJX48026.1		 B7Z5V6
P10253
P10253
Q8IWE7
5KZW[A] α-D-Glcp-(1-6)-β-D-Glcp
 2.00
5KZX[A] α-D-Glcp-(1-6)-β-D-Glcp
 2.00
5NN3[A] 1.90
5NN4[A] 1.83
5NN5[A] <carb_like_NOJ>
 2.00
5NN6[A] <carb_like_MIG>
 2.00
5NN8[A] <non_carb>-(1-4)-α-D-6-deoxy-Glcp4N
<non_carb>-(1-4)-α-D-6-deoxy-Glcp4N-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp
 2.45
7P2Z[A,B]
7P32[A]
8CB1[B] 1.75
8CB6[B] 1.90
1
 α-glucosidase II (GanAB;G2AN;KIAA0088 ) 3.2.1.20
3.2.1.207		 Homo sapiens AAF66685.1
AAH17433.2
AAH17435.2
AAH65266.1
ACJ13667.1
BAA07642.1
BAG58624.1
BAG58692.1
BAG61688.1
BAG63965.1
CAA04006.1
SJX47948.1
XP_043268.1		 B4DIW2
B4DJ30
B4DSM6
B4DZ53
Q14697
Q14697
V9HWJ0
8D43[A] 2.88
8EMR[A] 2.92
1
 maltase-glucoamylase (MgaM; Mga; MGAML) 3.2.1.20 Homo sapiens AAC39568.2
AAI20873.1
AAL83560.1
NP_004659.1		 O43451
Q8TE24
3TON[A,B] 2.95
3TOP[A,B] <non_carb>-(1-4)-α-D-6-deoxy-Glcp4N-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp
 2.88
1
 maltase-glucoamylase (MgaM; Mga; MGAML) 3.2.1.20 Homo sapiens AAC39568.2
AAI20873.1
AAL83560.1
AAP21875.1
NP_004659.1		 O43451
Q86UM5
Q8TE24
2QLY[A] 2.00
2QMJ[A] <non_carb>-(1-4)-α-D-6-deoxy-Glcp4N-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp
 1.90
3CTT[A] <carb_like_3CU>
 2.10
3L4T[A] <carb_like_BJ1>
 1.90
3L4U[A] <carb_like_DSK>
 1.90
3L4V[A] <carb_like_KTL>
 2.10
3L4W[A] <carb_like_MIG>
 2.00
3L4X[A] <carb_like_NR3>
 1.90
3L4Y[A] <carb_like_NR4>
 1.80
3L4Z[A] <carb_like_SSD>
 2.00
1
 sucrase-isomaltase (SI) 3.2.1.10 Homo sapiens AAT19036.1
AAA60551.1
AAI15035.1
AAI16453.1
AAI16454.1
AAI32835.1
AAI32861.1
AAT18166.1
ABH87017.1
ACM85129.1
ACP58672.1
CAA45140.1
NP_001032.1		 P14410
3LPO[A,B,C,D] α-D-Manp-(1-6)[α-D-Manp-(1-3)]-β-D-Manp-(1-4)-β-D-GlcpNAc-(1-4)-D-1-deoxy-GlcpNAc
 3.20
3LPP[A,B,C,D] <carb_like_KTL>
 2.15
1
 α-galactosidase (MYORG; KIAA1161; MGC:87539) 3.2.1.22 Homo sapiens AAH70098.1
AAI10494.1
BAA86475.2
CAI15965.1
SJX44446.1		 Q6NSJ0
7QQF[A,B,C,D] 2.43
7QQG[A,B,C,D] <carb_like_DGJ>
 2.43
7QQH[A,B,C,D] α-D-Galp-(1-4)-β-D-Glcp
 2.25
20
 α-glucosidase II (α-GluII;AB;GanAB;GANAB;G2an;Kiaa0088 )   Mus musculus AAC53182.1
AAH94437.1
AAI17889.1
AAI17890.1
AAI28070.1
BAB30982.1
BAC27099.1
BAC38370.1
BAC65483.1
BAE29025.1
NP_032086.1		  
5F0E[A] 1.74
5H9O[A,C] α-D-Glcp
 2.37
5HJO[A,C] α-D-2-deoxy-Glcp-(1-3)-D-1,2-deoxy-Glcp
D-1,2-deoxy-Glcp
 2.29
5HJR[A,C] β-D-Glcp5F-(1-4)-ASP
 2.40
5IED[A] <carb_like_CTS>
 1.81
5IEE[A] <carb_like_NOJ>
 1.92
5IEF[A] <carb_like_NBV>
 2.38
5IEG[A] 1.82
7JTY[A,C] 2.21
7K9N[A,C] 2.07
7K9O[A,C] 2.30
7K9Q[A,C] 2.30
7K9T[A,C] 2.10
7KAD[A,C] 2.51
7KB6[A,C] 2.20
7KB8[A,C] 2.39
7KBJ[C,G] 2.21
7KBR[C,G] 2.09
7KRY[A,C] 2.55
7L9E[C,G] 2.29
1
 α-1,3-glucosidase II α-subunit (GIIa;CTHT_0064960) 3.2.1.84 Thermochaetoides thermophila DSM 1495 EGS17181.1 G0SG42
5DKX[A] 1.40
5DKY[A] <carb_like_NOJ>
 1.60
5DKZ[A] α-D-Glcp-(1-3)-α-D-Glcp
 2.40
5DL0[A] α-D-Glcp-(1-3)-α-D-Manp
 2.30
5JQP[A] 2.20
1
unclassified
Protein Name EC#OrganismGenBankUniprot
PDB/3D Carbohydrate Ligands Resolution (Å)
 Subf
 α-xylosidase (MeXyl31) 3.2.1.177 uncultured microorganism BAU88419.1 A0A169PW02
5ZN6[A,B,C,D,E,F,G,H] 1.80
5ZN7[A,B,C,D,E,F,G,H] α-D-Xylp
 2.10
4
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