Mechanism	Retaining e
Clan	GH-K
3D Structure Status	( β / α ) 8 barrel
Catalytic Nucleophile/Base	carbonyl oxygen of C-2 acetamido group of substrate
Catalytic Proton Donor	Glu
External resources	CAZypedia; Tay-Sachs Disease;
Commercial Enzyme Provider(s)	MEGAZYME;
Statistics	GenBank accession (24333); Uniprot accession (191); PDB accession (122); 3D entries (38); cryst (0)

Bacteria
Protein Name	EC#	Organism	GenBank	Uniprot	PDB/3D Carbohydrate Ligands Resolution (Å)
dispersin B / β-1,6-N-acetylglucosaminidase (DspB)	3.2.1.-	Aggregatibacter actinomycetemcomitans CU1000	AAP31025.1	Q840G9	1YHT[A] 2.00
β-N-acetylhexosaminidase (Am 2446; Amuc_0369)	3.2.1.52	Akkermansia muciniphila ATCC BAA-835	ACD04208.1 QTD81022.1		8PXT[A,B,C] β-D-GalpNAc 2.25 8PXU[A,B,C] 1.99 8PXV[A,B,C] β-D-Galp-(1-4)-β-D-Glcp 2.50
β-N-acetylhexosaminidase (Am 0986; Amuc_0868)	3.2.1.52	Akkermansia muciniphila ATCC BAA-835	ACD04701.1 QTD81489.1	B2UQG6	7CBN[A] 1.70 7CBO[A] β-D-GlcpNAc 1.50
β-N-acetylhexosaminidase (Am 2301; Amuc_2018)	3.2.1.52	Akkermansia muciniphila ATCC BAA-835	ACD05828.1 QTD80528.1	B2UP57	6JE8[A] 1.80 6JEA[A] β-D-GlcpNAc 2.28 6JEB[A] 1.50
β-N-acetylhexosaminidase (Am2136; Am 0421; Amuc_2136)	3.2.1.52	Akkermansia muciniphila ATCC BAA-835	ACD05945.1 QTD80640.1	B2UPR7	6JQF[A] β-D-GlcpNAc 1.90 7EZT[A,B,C,D] 2.81
β-N-acetylhexosaminidase (BF3095; BF9343_3009)		Bacteroides fragilis NCTC 9343	CAH08790.1		4PYS[A,B] 1.82
β-N-acetyl-6-sulfo-glucosaminidase (Btheta7330_03706)	3.2.1.-	Bacteroides thetaiotaomicron 7330	ALJ43236.1	A0A0P0FIE8 Q89ZI3	7DUP[A] 1.62 7DVA[A,B] β-D-GlcpNAc6SO3 1.55 7DVB[A,B,C,D] β-D-GlcpNAc6SO3 2.05 8BDP[A] 1.47
β-N-acetylglucosaminidase (BT0459; BT_0459)	3.2.1.52	Bacteroides thetaiotaomicron VPI-5482	AAO75566.1 NP_809372.1	A0A174QSL3 Q8AAK5	6Q63[A,B,C] β-D-GlcpNAc 2.44
β-N-acetyl-6-sulfo-glucosaminidase (BbhII)	3.2.1.-	Bifidobacterium bifidum JCM 1254	BAI94823.1	D4QAP5	6YXZ[A] 6Z14[A] 7WDT[A] α-D-GlcpNAc6SO3 β-D-GlcpNAc6SO3 1.65 7WDU[A] α-D-Glcp1N2NAc6SO3-(1-1)-<non_carb> 2.23
lacto-N-biosidase (LnbB)	3.2.1.140	Bifidobacterium bifidum JCM 1254	ABZ78855.1	B3TLD6	4H04[A,B] β-D-Galp-(1-3)-β-D-GlcpNAc 1.80 4JAW[A,B] 1.80 5BXP[A,B] β-D-Galp-(1-3)-α-D-Glcp1N2NAc-(1-1)-<non_carb> β-D-Galp-(1-3)-β-D-Glcp1N2NAc-(1-1)-<non_carb> 1.70 5BXR[A,B] β-D-Galp-(1-1)-<non_carb> <carb_like_NOK> 1.60 5BXS[A,B] β-D-Galp-(1-1)-<non_carb> <carb_like_GC2> 2.20 5BXT[A,B] β-D-Galp-(1-1)-<non_carb> 1.80
endoglycosidase (EndoE;NdoE)	3.2.1.96	Enterococcus faecalis HER1044	AAR20477.1	Q6U890 Q839P8	7PUL[A] 1.40
β-N-acetylhexosaminidase (FjGH20; Fjoh_4556)	3.2.1.52	Flavobacterium johnsoniae UW101	ABQ07555.1	A5FB64	6YHH[A,B] 1.70
dispersin (DispLp;DA077_06060)	3.2.1.-	Lactiplantibacillus paraplantarum AS-7	AVW10117.1		8QCE[A,B] <carb_like_GC2> 1.05
β-N-acetylhexosaminidase (HJ5Nag)	3.2.1.52	Microbacterium sp. HJ5	ARJ33352.1	A0A1W6AQA7	7BWG[A,B] 2.85
β-N-acetyl-6-sulfo-glucosaminidase (Niako_3494)	3.2.1.-	Niastella koreensis GR20-10	AEV99795.1	G8TKW6	8BAL[A,B,C,D,E] 2.27
β-N-acetylhexosaminidase (AAur_4089)		Paenarthrobacter aurescens TC1	ABM09106.1		3RCN[A] 2.51
β-hexosaminidase (Hex1T)	3.2.1.52	Paenibacillus sp. TS12	BAI63641.1	D0VX21 D2KW09	3GH4[A] 1.80 3GH5[A] β-D-GlcpNAc 1.60 3GH7[A] β-D-GalpNAc 1.90 3SUR[A] 1.90 3SUS[A] D-1,2-deoxy-Galp 1.80 3SUT[A] α-D-Glcp1N2NAc-(1-1)-<non_carb> 1.90 3SUU[A] α-D-Galp1N2NAc-(1-1)-<non_carb> 1.60 3SUV[A] <carb_like_NOK> 1.60 3SUW[A] <carb_like_GC2> 1.90
mucin-desulfating glycosidase / sulfoglycosidase / β-6-SO3-N-acetylglucosaminidase (SGL)	3.2.1.-	Prevotella sp. RS2	AAW30398.1	Q5MAH5	8BBL[A,B,C,D,E,F,G,H] 2.71
chitobiase (Chb;Ctb;SmCHB;SmGH20A)	3.2.1.52	Serratia marcescens 2170	AAB03808.1	Q54468	1C7S[A] β-D-GlcpNAc-(1-4)-β-D-GlcpNAc 1.80 1C7T[A] β-D-GlcpNAc-(1-4)-β-D-GlcpNAc 1.90 1QBA[A] 1.85 1QBB[A] β-D-GlcpNAc-(1-4)-α-D-GlcpNAc 2.00
β-N-acetylglucosaminidase (GcnA)	3.2.1.52	Streptococcus gordonii FSS2	AAS02154.1	Q6ST21	2EPK[X] 1.85 2EPL[X] 1.40 2EPM[X] 2.04 2EPN[A,B] 1.61 2EPO[A,B] 1.56
β-N-acetylhexosaminidase (StrH;spr0057)	3.2.1.52	Streptococcus pneumoniae R6	AAK98861.1 NP_357651.1	Q8DRL6	3RPM[A] β-D-GlcpNAc 2.10
β-N-acetylhexosaminidase (StrH;spr0057)	3.2.1.52	Streptococcus pneumoniae R6	AAK98861.1 NP_357651.1	Q8DRL6	3RPM[A,B] β-D-GlcpNAc 2.10
β-N-acetylhexosaminidase (GH20C;SP2141;SP_2141)	3.2.1.52	Streptococcus pneumoniae TIGR4	AAK76198.1	A0A0H2US73	5A69[A] β-D-Galp1N2NAc-(1-1)-<non_carb> 2.20 5A6A[A,B] 2.69 5A6B[A,B,C,D] β-D-Glcp1N2NAc-(1-1)-<non_carb> 1.77 5A6J[A,B,C,D] 1.94 5A6K[A,B] D-1,2-deoxy-Galp 2.10 5AC4[A,B] β-D-GalpNAc 2.08 5AC5[A,B] β-D-GlcpNAc 1.84
β-N-acetylglucosaminidase (StrH;SP0057;SP_0057)	3.2.1.52	Streptococcus pneumoniae TIGR4	AAK74246.1 NP_344606.1	P49610	2YL6[A] 1.60 2YL8[A] β-D-GlcpNAc-(1-2)-α-D-Manp 1.75 2YLL[A] 1.85 4AZ5[A] 1.73 4AZ6[A] α-D-Glcp1N2NAc-(1-1)-<non_carb> 1.36 4AZ7[A] β-D-Glcp1N2NAc-(1-1)-<non_carb> 1.70 4AZB[A] α-D-Glcp1N2NAc-(1-1)-<non_carb> 2.10
β-N-acetylglucosaminidase (StrH;SP0057;SP_0057)	3.2.1.52	Streptococcus pneumoniae TIGR4	AAK74246.1 NP_344606.1	P49610	2YL5[A,B,C,D] 2.15 2YL9[A,B,C,D] β-D-GlcpNAc-(1-2)-α-D-Manp-(1-3)-β-D-Manp-(1-4)-β-D-GlcpNAc 2.65 2YLA[A,B,C,D] β-D-GlcpNAc-(1-2)-α-D-Manp-(1-3)-β-D-Manp-(1-4)-β-D-GlcpNAc β-D-GlcpNAc-(1-2)-α-D-Manp-(1-3)[β-D-GlcpNAc-(1-4)]-β-D-Manp-(1-4)-β-D-GlcpNAc 2.70 4AZC[A,B,C,D] 2.09 4AZG[A,B] α-D-Glcp1N2NAc-(1-1)-<non_carb> β-D-Glcp1N2NAc-(1-1)-<non_carb> 2.40 4AZH[A,B,C,D] β-D-Glcp1N2NAc-(1-1)-<non_carb> 2.22 4AZI[A,B] α-D-Glcp1N2NAc-(1-1)-<non_carb> β-D-Glcp1N2NAc-(1-1)-<non_carb> 1.98
β-N-acetylhexosaminidase (HexA;SCO2786)	3.2.1.52	Streptomyces coelicolor A3(2)	CAB87223.1 NP_627016.1	Q9L068	4C7D[A,B] 1.85 4C7F[A,B] <carb_like_GC2> 2.00 4C7G[A] 1.80
β-N-acetylglucosaminidase (Hex; SpHex)	3.2.1.52	Streptomyces plicatus	AAC38798.3	O85361	1HP4[A] 2.20 1HP5[A] 2.10 1JAK[A] <carb_like_IFG> 1.75 1M01[A] β-D-GlcpNAc 2.10 1M03[A] β-D-GlcpNAc 1.90 1M04[A] β-D-GlcpNAc 1.95 5FCZ[A] <non_carb>-(1-2)-D-1-deoxy-GlcpN 2.45 5FD0[A] β-D-GlcpNAc 2.00
lacto-N-biosidase / N-acetyl-lactosaminidase (LnbS)	3.2.1.140	Streptomyces sp. 142	AAD10478.1 AAE24545.1	Q9Z4I7	8HVB[A] β-D-Galp-(1-3)-α-D-GlcpNAc 1.60 8HVC[A] β-D-Galp-(1-3)-β-D-GalpNAc 1.58 8HVD[A] β-D-Galp-(1-3)-α-D-GalpNAc 1.41
Dispersin (DispTs2)	3.2.1.-	Terribacillus saccharophilus			9HTA[A] 2.17 8QB6[A] <carb_like_GC2> 1.51
dispersin GH20 (DispTs3)	3.2.1.-	Terribacillus saccharophilus NZ83GA	WRI53132.1		8QAK[A,B] 1.95
N‐acetyl-lactosaminidase (TrdeGH20; TDE_1723)		Treponema denticola ATCC 35405	AAS12238.1		9FYL[A,B] 2.24 9FYM[A] 1.34
lacto-N-biosidase (TrpyGH20; CQ11_10535)	3.2.1.140	Trueperella pyogenes TP6375	AHU90310.1		9FYN[A] 1.79 9FYO[A] β-D-Galp-(1-3)-β-D-GlcpNAc 2.77
β-N-acetylglucosaminidase (Nag2; VhNag2; VhGlcNAcase)	3.2.1.52	Vibrio harveyi 650	ADJ68333.1	D9ISE0	6EZR[A,B] 2.37 6EZS[A,B] β-D-GlcpNAc 2.50 6EZT[A,B] 2.60 6K35[A,B] 2.36
Eukaryota
Protein Name	EC#	Organism	GenBank	Uniprot	PDB/3D Carbohydrate Ligands Resolution (Å)
β-N-acetylhexosaminidase (HexA)	3.2.1.52	Aspergillus oryzae CCF 1066	AAM13977.1	Q8J2T0	5OAR[B,D] 2.30
β-N-acetylglucosaminidase / β-hexosaminidase A (α-chain) (HexA)	3.2.1.52	Homo sapiens	AAB00965.1 AAA51827.1 AAD13932.1 AAH01138.2 AAH18927.1 AAH21030.1 AAH84537.1 AAQ70000.1 ACM85216.1 ALQ33567.1 ALQ33569.1 ALQ33570.1 BAD96222.1 BAG59159.1 BAG60039.1 BAG62619.1 BAG62730.1 CAH10482.1 NP_000511.1	A0A0S2Z3L7 A0A0S2Z3U8 A0A0S2Z3W3 B4DVA7 B4DVL8 P06865 Q9BVJ8	2GJX[A,D,E,H] 2.80 2GK1[A,C,E,G] 3.25
β-N-acetylglucosaminidase B (β-chain) (HexB)	3.2.1.52	Homo sapiens	AAA52645.1 AAA51828.1 AAA51829.1 AAA68620.1 AAH17378.1 AAM46114.1 AAP88921.1 AAV48875.1 ACI46028.1 ACV87197.1 ADO22272.1 NP_000512.1	A0A024RAJ6 P07686 Q5URX0	1NOU[A,B] 2.40 1NOW[A,B] <carb_like_IFG> 2.20 1NP0[A,B] 2.50 1O7A[A,B,C,D,E,F] α-D-GlcpNAc β-D-GlcpNAc 2.25 2GJX[B,C,F,G] 2.80 2GK1[B,D,F,H] 3.25 3LMY[A,B] 2.80 5BRO[A] 2.40
β-N-acetylhexosaminidase (Hex1; OfHex1)	3.2.1.52	Ostrinia furnacalis	ABI81756.1	Q06GJ0	3NSM[A] 2.10 3NSN[A] β-D-Glcp2NMe-(1-4)-β-D-GlcpNAc-(1-4)-β-D-GlcpNAc-(1-4)-β-D-GlcpNAc 2.10 3OZO[A] 2.00 3OZP[A] α-D-Glcp1N2NAc-(1-1)-<non_carb> 2.00 3S6T[A] β-D-Glcp1N2NAc-(1-1)-<non_carb> 2.30 3VTR[A] β-D-Glcp2NMe-(1-4)-β-D-GlcpNAc-(1-4)-β-D-GlcpNAc-(1-4)-β-D-GlcpNAc D-1-deoxy-GlcpNAc 2.50 3WMB[A] 2.70 3WMC[A] 2.10 5Y0V[A] 2.42 5Y1B[A] 2.21
β-N-acetylhexosaminidase (HEX-2; TsHEX-2; M514_07548)	3.2.1.52	Trichuris suis	KFD67184.1	A0A085NCI8	8QK1[A] 2.55
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