Glycoside Hydrolase Family 98

Activities in Family	blood-group endo-β-1,4-galactosidase (EC 3.2.1.102); endo-β-1,4-xylanase (EC 3.2.1.8)
Mechanism	Inverting
3D Structure Status	( α / β ) ₈ barrel
Catalytic Nucleophile/Base	Asp+Glu diad
Catalytic Proton Donor	Glu
Note	Created after Anderson et al. (J. Biol. Chem. 2005 280: 7720-7728; PMID:15618227)
External resources	CAZypedia;
Statistics	GenBank accession (511); Uniprot accession (10); PDB accession (19); 3D entries (3); cryst (0)

Bacteria

Protein Name

EC#

Organism

GenBank

Uniprot

PDB/3D

Carbohydrate Ligands

Resolution (Å)

blood-group endo-β-1,4-galactosidase (RgGH98;FXV78_04990)

endo-β-1,4-galactosidase (CGSSp3BS71_10403;Sp3GH98;EABase) (blood-group A/B-antigen-specific)

2WMI[A,B]	α-D-GalpNAc-(1-3)[α-L-Fucp-(1-2)]-β-D-Galp	1.90
2WMJ[A,B]	α-D-Galp-(1-3)[α-L-Fucp-(1-2)]-β-D-Galp	2.00
2WMK[A,B]	α-D-GalpNAc-(1-3)[α-L-Fucp-(1-2)]-β-D-Galp-(1-4)[α-L-Fucp-(1-3)]-β-D-GlcpNAc	1.90
4D6C[A]		1.59
4D6D[A]	α-D-GalpNAc-(1-3)[α-L-Fucp-(1-2)]-α-D-Galp	1.52
4D6E[A]	α-D-GalpNAc-(1-3)[α-L-Fucp-(1-2)]-β-D-Galp	1.45
4D6F[A]	α-D-GalpNAc-(1-3)[α-L-Fucp-(1-2)]-β-D-Galp-(1-3)-β-D-GlcpNAc	2.03
4D6G[A]	α-D-GalpNAc-(1-3)[α-L-Fucp-(1-2)]-α-D-Galp	1.24
4D6H[A]	α-D-GalpNAc-(1-3)[α-L-Fucp-(1-2)]-β-D-Galp-(1-3)-β-D-GlcpNAc	1.65
4D6I[A]	α-D-GalpNAc-(1-3)[α-L-Fucp-(1-2)]-β-D-Galp-(1-3)-β-D-GlcpNAc	1.99
4D6J[A]	α-D-GalpNAc-(1-3)[α-L-Fucp-(1-2)]-β-D-Galp-(1-4)-β-D-GlcpNAc	1.98
4D71[A]	α-D-GalpNAc-(1-3)[α-L-Fucp-(1-2)]-β-D-Galp-(1-4)-β-D-GlcpNAc	1.77
4D72[A]	α-D-GalpNAc-(1-3)[α-L-Fucp-(1-2)]-β-D-Galp-(1-4)-β-D-GlcpNAc	2.11

endo-β-1,4-galactosidase (SP_2159;SP2159;Sp4GH98) (LewisY-specific)

AAK76213.1
AAY71778.1
NP_346573.1

2WMF[A]		1.50
2WMG[A]	α-L-Fucp-(1-2)-β-D-Galp-(1-4)[α-L-Fucp-(1-3)]-β-D-GlcpNAc	2.30
2WMH[A]	α-L-Fucp-(1-2)-β-D-Galp	1.70