Glycoside Hydrolase Family 5 / Subf 25

Activities in Sub FamilyEndo-β-1,4-glucanase (EC 3.2.1.4);
Endo-β-1,4-mannanase (EC 3.2.1.78);
Endo-β-1,4-xylanase (EC 3.2.1.8);
Mechanism Retaining e
ClanGH-A
3D Structure Status( β / α ) 8 barrel
Catalytic Nucleophile/BaseGlu (experimental)
Catalytic Proton DonorGlu (experimental)
NoteOnce known as cellulase family A; many members have been assigned to subfamilies as described by Aspeborg et al. (2012) BMC Evol Biol. 12(1):186 (PMID: 22992189).
External resourcesCAZypedia; HOMSTRAD; PROSITE;
Commercial Enzyme Provider(s)MEGAZYME; NZYTech; PROZOMIX;
Statistics GenBank accession (505); Uniprot accession (15); PDB accession (24); 3D entries (6); cryst (0)
Bacteria
Protein Name EC# OrganismGenBankUniprot
PDB/3D Carbohydrate Ligands Resolution (Å)
Subf
 bifunctional endo-β-1,4-glucanase / β-1,4-xylanase H (CelH;EgH;CtCelH;CtCel5E;Cthe_1472) (Lic26A;Cel5E) 3.2.1.4
3.2.1.8
Acetivibrio thermocellus ATCC 27405 ABN52701.1
CCF82162.1
H6SHY4
P16218
4U3A[A,B] 2.42
4U5I[A,B] β-D-Xylp-(1-4)-β-D-Xylp
2.50
4U5K[A,B] β-D-Glcp-(1-4)-β-D-Glcp
2.65
5BYW[A,B,C,D,E] 2.60
25
 CDIF28668_02723 3.2.1.4 Clostridioides difficile DSM 28668 AXU72614.1 A0A5Q9L120
6UJE[A] 1.75
6UJF[A] β-D-Glcp
2.00
25
 endo-β-1,4-glucanase (FnCel5A;Fnod_1560) (Cel5A) 3.2.1.4 Fervidobacterium nodosum Rt17-B1 ABS61403.1
ADD73709.1
A7HNC0
D4PEB3
3NCO[A,B] 1.50
3RJX[A] 2.40
3RJY[A] α-D-Glcp
2.20
25
 endoglucanase (BcelFp; Ferpe_1841) 3.2.1.4 Fervidobacterium pennivorans DSM 9078 AFG35892.1  
6KDD[A] 1.92
25
 endo-β-1,4-glucanase (MtGlu5; Mtai_v1c27890)   Meiothermus taiwanensis WR-220 AWR88013.1  
7VT4[A,B] 1.93
7VT5[A] 1.46
7VT6[A] β-D-Glcp
1.53
7VT7[A] β-D-Glcp-(1-4)-β-D-Glcp
1.53
7VT8[A] β-D-Glcp
2.99
25
 endo-β-1,4-glucanase (MtGlu5; Mtai_v1c27890) 3.2.1.4 Meiothermus taiwanensis WR-220 AWR88013.1  
7VT4[A,B] 1.93
7VT5[A] 1.46
7VT6[A] β-D-Glcp
1.53
7VT7[A] β-D-Glcp-(1-4)-β-D-Glcp
1.53
7VT8[A] β-D-Glcp
2.99
25
 endoglucanase / β-mannanase (Tm_Cel5A; TmCel5A; TM1751) (Cel5A) 3.2.1.4
3.2.1.78
Thermotoga maritima MSB8 AAD36816.1
AGL50683.1
AHD18355.1
AKE29506.1
NP_229549.1
Q9X273
R4P461
3AMC[A,B] 1.40
3AMD[A,B,C,D] 2.00
3AMG[A,B] β-D-Glcp
β-D-Glcp-(1-4)-β-D-Glcp
2.40
3AOF[A,B] β-D-Manp-(1-4)-β-D-Manp-(1-4)-β-D-Manp
1.29
3AZR[A,B] β-D-Glcp
β-D-Glcp-(1-4)-α-D-Glcp
1.71
3AZS[A,B] β-D-Manp-(1-4)-β-D-Manp-(1-4)-α-D-Manp
1.69
3AZT[A,B,C,D] β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-α-D-Glcp
1.80
3MMU[A,B,C,D,E,F,G,H] 2.20
3MMW[A,B,C,D] 1.85
25
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