Activities in Sub Family	Endo-β-1,4-glucanase (EC 3.2.1.4); Endo-β-1,4-mannanase (EC 3.2.1.78); Endo-β-1,4-xylanase (EC 3.2.1.8);
Mechanism	Retaining e
Clan	GH-A
3D Structure Status	( β / α ) 8 barrel
Catalytic Nucleophile/Base	Glu (experimental)
Catalytic Proton Donor	Glu (experimental)
Note	Once known as cellulase family A; many members have been assigned to subfamilies as described by Aspeborg et al. (2012) BMC Evol Biol. 12(1):186 (PMID: 22992189).
External resources	CAZypedia; HOMSTRAD; PROSITE;
Commercial Enzyme Provider(s)	MEGAZYME; NZYTech; PROZOMIX;
Statistics	GenBank accession (515); Uniprot accession (15); PDB accession (24); 3D entries (6); cryst (0)

Bacteria
Protein Name	EC#	Organism	GenBank	Uniprot	PDB/3D Carbohydrate Ligands Resolution (Å)	Subf
bifunctional endo-β-1,4-glucanase / β-1,4-xylanase H (CelH;EgH;CtCelH;CtCel5E;Cthe_1472) (Lic26A;Cel5E)	3.2.1.4 3.2.1.8	Acetivibrio thermocellus ATCC 27405	ABN52701.1 CCF82162.1	H6SHY4 P16218	4U3A[A,B] 2.42 4U5I[A,B] β-D-Xylp-(1-4)-β-D-Xylp 2.50 4U5K[A,B] β-D-Glcp-(1-4)-β-D-Glcp 2.65 5BYW[A,B,C,D,E] 2.60	25
cellulose endo-β-1,4-glucosidase (CDIF28668_02723)	3.2.1.4	Clostridioides difficile DSM 28668	AXU72614.1	A0A5Q9L120	6UJE[A] 1.75 6UJF[A] β-D-Glcp 2.00	25
endo-β-1,4-glucanase (FnCel5A;Fnod_1560) (Cel5A)	3.2.1.4	Fervidobacterium nodosum Rt17-B1	ABS61403.1 ADD73709.1	A7HNC0 D4PEB3	3NCO[A,B] 1.50 3RJX[A] 2.40 3RJY[A] α-D-Glcp 2.20	25
endoglucanase (BcelFp; Ferpe_1841)	3.2.1.4	Fervidobacterium pennivorans DSM 9078	AFG35892.1		6KDD[A] 1.92	25
endo-β-1,4-glucanase (MtGlu5; Mtai_v1c27890)		Meiothermus taiwanensis WR-220	AWR88013.1		7VT4[A,B] 1.93 7VT5[A] 1.46 7VT6[A] β-D-Glcp 1.53 7VT7[A] β-D-Glcp-(1-4)-β-D-Glcp 1.53 7VT8[A] β-D-Glcp 2.99	25
endo-β-1,4-glucanase (MtGlu5; Mtai_v1c27890)	3.2.1.4	Meiothermus taiwanensis WR-220	AWR88013.1		7VT4[A,B] 1.93 7VT5[A] 1.46 7VT6[A] β-D-Glcp 1.53 7VT7[A] β-D-Glcp-(1-4)-β-D-Glcp 1.53 7VT8[A] β-D-Glcp 2.99	25
endoglucanase / β-mannanase (Tm_Cel5A; TmCel5A; TM1751) (Cel5A)	3.2.1.4 3.2.1.78	Thermotoga maritima MSB8	AAD36816.1 AGL50683.1 AHD18355.1 AKE29506.1 NP_229549.1	Q9X273 R4P461	3AMC[A,B] 1.40 3AMD[A,B,C,D] 2.00 3AMG[A,B] β-D-Glcp β-D-Glcp-(1-4)-β-D-Glcp 2.40 3AOF[A,B] β-D-Manp-(1-4)-β-D-Manp-(1-4)-β-D-Manp 1.29 3AZR[A,B] β-D-Glcp β-D-Glcp-(1-4)-α-D-Glcp 1.71 3AZS[A,B] β-D-Manp-(1-4)-β-D-Manp-(1-4)-α-D-Manp 1.69 3AZT[A,B,C,D] β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-α-D-Glcp 1.80 3MMU[A,B,C,D,E,F,G,H] 2.20 3MMW[A,B,C,D] 1.85	25
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