Glycoside Hydrolase Family 32

Activities in Familyinvertase (EC 3.2.1.26); endo-inulinase (EC 3.2.1.7); β-2,6-fructan 6-levanbiohydrolase (EC 3.2.1.64); endo-levanase (EC 3.2.1.65); exo-inulinase (EC 3.2.1.80); fructan β-(2,1)-fructosidase/1-exohydrolase (EC 3.2.1.153); fructan β-(2,6)-fructosidase/6-exohydrolase (EC 3.2.1.154); sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99); fructan:fructan 1-fructosyltransferase (EC 2.4.1.100); sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10); fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243); levan fructosyltransferase (EC 2.4.1.-); [retaining] sucrose:sucrose 6-fructosyltransferase (6-SST) (EC 2.4.1.-); cycloinulo-oligosaccharide fructanotransferase (EC 2.4.1.-)
Mechanism Retaining
ClanGH-J
3D Structure Status5-fold β-propeller
Catalytic Nucleophile/BaseAsp
Catalytic Proton DonorGlu
External resourcesCAZypedia; PROSITE;
Commercial Enzyme Provider(s)MEGAZYME;
Statistics GenBank accession (21536); Uniprot accession (284); PDB accession (78); 3D entries (23); cryst (2)
| 1 | 2 | 3 |
Bacteria
Protein Name EC#ReferenceOrganismGenBank UniprotPDB/3D
 invertase (LevJ) 3.2.1.26 pubmed
pubmed
Actinomyces naeslundii T14V AAA67876.1 Q44109  
 invertase (LevJ) 3.2.1.26 pubmed
pubmed
Actinomyces naeslundii T14V AAA67876.1 Q44109  
 endo-inulinase (Inu1) 3.2.1.7 pubmed
Arthrobacter sp. S37 CAB63119.1 Q9RBA5  
 endolevanase (LevB1) (partial) 3.2.1.65 doi
Bacillus licheniformis IBT-ALM / IBt1 AHK25455.1 W8GV60  
 endo-levanase 3.2.1.65 pubmed
Bacillus sp. L7 CAA73180.1 O31411  
 invertase (SacA;BSU38040;IPA-50D) 3.2.1.26 pubmed
Bacillus subtilis subsp. subtilis str. 168 AAA22723.1
ABV89908.1
AIY95118.1
AQR83843.1
AQR88058.1
CAA51606.1
CAB15830.1
CAB15830.2
NP_391683.1
A8HU42
P07819
 
 levanase (SacC;BSU27030) 3.2.1.65 pubmed
Bacillus subtilis subsp. subtilis str. 168 CAB14645.1
AIY94027.1
AQR82724.1
AQR86939.1
CAA29137.1
CAA63465.1
CAA68542.1
NP_390581.1
A0A6M3ZED6
P05656
 
 endo-levanase (YveB;LevB;BSU34460) 3.2.1.65 pubmed
Bacillus subtilis subsp. subtilis str. 168 CAB08014.1
AIY94762.1
AQR83480.1
AQR87695.1
CAB15451.1
NP_391326.1
A0A6M4JPE5
O07003
 
 β-fructanase (FruA;ScrL) 3.2.1.26
3.2.1.64
3.2.1.65
pubmed
pubmed
pubmed
Bacteroides fragilis BF-1 AAA22924.1 Q45155  
 β-fructofuranosidase (BT1759) 3.2.1.153
3.2.1.26
3.2.1.64
pubmed
pubmed
pubmed
Bacteroides thetaiotaomicron VPI-5482 AAO76866.1
NP_810672.1
Q8A6W7  
 β-fructofuranosidase (BT3082;BT_3082) 3.2.1.153
3.2.1.26
3.2.1.64
pubmed
pubmed
pubmed
Bacteroides thetaiotaomicron VPI-5482 AAO78188.1
NP_811994.1
Q8A373  
 endo-β-2,6-fructanase / endo-levanase (BT1760;BT_1760) 3.2.1.65 pubmed
Bacteroides thetaiotaomicron VPI-5482 AAO76867.1
NP_810673.1
Q8A6W6 6R3R[A]
6R3U[A]
 β-fructofuranosidase (BT1765) 3.2.1.153
3.2.1.26
3.2.1.64
pubmed
pubmed
pubmed
Bacteroides thetaiotaomicron VPI-5482 AAO76872.1
NP_810678.1
Q8A6W1  
 β-fructofuranosidase (CscA) 3.2.1.26 pubmed
Bifidobacterium adolescentis G1 BAJ21238.1 E1CPU4  
 β-fructosidase (BfrA) 3.2.1.26 pubmed
Bifidobacterium animalis subsp. lactis DSM 10140(T) CAD26970.1
AAS87041.1
Q564C7
Q84F22
 
 β-fructofuranosidase (FosC; Bbr_1324) 3.2.1.26 pubmed
Bifidobacterium breve UCC2003 AAT28190.1
ABE95999.1
F9Y237
Q4JEX8
 
 β-fructofuranosidase 3.2.1.26 doi
Bifidobacterium longum KN29.1 ABN04092.1 A2TLS9 3PIG[A,B]
3PIJ[A,B]
 sucrose-6-P hydrolase (ScrB;SacA;CAC0425) 3.2.1.26 pubmed
Clostridium acetobutylicum ATCC 824 AAF35841.1
AAK78405.1
NP_347065.1
Q7D471
Q9L8G4
 
 invertase 3.2.1.26 pubmed
Clostridium beijerinckii AAC99322.1 Q9ZHJ7  
 sucrose-6-P hydrolase (ScrB;Cgl2643;Cg2643) 3.2.1.26 pubmed
Corynebacterium glutamicum ATCC 13032 BAC00037.1
CAF21305.1
CCH25774.1
NP_601843.1
Q8NMD5  
 sucrose hydrolase (CscA) 3.2.1.26 pubmed
Escherichia coli AAP79503.1 O86076  
 invertase (CscA) 3.2.1.26 pubmed
Escherichia coli B-62 AAC33123.1 O86076  
 invertase (CscA) 3.2.1.26 pubmed
Escherichia coli EC3132 CAA57219.1 P40714  
 β-fructofuranosidase (FperFFase;FPB0191_01269) 3.2.1.26 pubmed
Frischella perrara PEB0191 AJA45090.1
BCZ08027.1
A0A0A7S0J1 7VCO[A]
7VCP[A]
 levanase (SurC) 3.2.1.65 pubmed
Geobacillus stearothermophilus ATCC 12980 AAB97112.1 P94469  
 exo-inulinase (InuA) 3.2.1.153 pubmed
Geobacillus stearothermophilus KP1289 BAC45010.1 Q8GI55 cryst
 invertase (SurA) 3.2.1.26 pubmed
Geobacillus stearothermophilus NUB36 AAB38976.1 P94466  
 levanase (LsdB) 3.2.1.65 doi
Gluconacetobacter diazotrophicus SRT4 CBS 550.94 AAF16405.1 Q9RBJ1  
 invertase (ScrB) 3.2.1.26 pubmed
Klebsiella pneumoniae 1033-5P14 CAA40659.1
CAA47976.1
P27217  
 inulinase / fructan hydrolase (LevH1) 3.2.1.153 pubmed
Lacticaseibacillus casei IAM 1045 BAD88632.1 Q5H7T0  
 β-fructosidase (FosE) 3.2.1.65 pubmed
Lacticaseibacillus paracasei 1195 ABD57319.1 Q27J21  
 fructan β-fructosidase / inulinase (Inu;InuB41) 3.2.1.7 pubmed
Lacticaseibacillus paracasei subsp. paracasei DSM 23505 AKA87712.1 A0A0E3KC98  
 β-fructofuranosidase (SacA;LPST_C0153) 3.2.1.26 doi
Lactiplantibacillus plantarum ST-III ADN97377.1 E1TRE0  
 sucrose-6-phosphate hydrolase 3.2.1.26 pubmed
Lactobacillus gasseri WP_003655369.1
EFB62189.1
A0A087Q9Y6
A0A806A0W7
A0A833CD48
D1YK18
6NU7[A]
6NU8[A]
 invertase (ScrB;SacA) 3.2.1.26 pubmed
Lactococcus lactis NIZO R5 AAA25218.1
CAB09689.1
Q04937
Q797W8
 
 2,6-β-fructan 6-levanbiohydrolase (LevM) 3.2.1.64 pubmed
Microbacterium laevaniformans ATCC 15953 BAB59060.1 Q93R69  
 levan fructotransferase (LftM) 4.2.2.16 pubmed
Microbacterium sp. AL-210 AAG01554.1 Q9EVQ9  
 β-fructofuranosidase (SacA;FF33) 3.2.1.26 pubmed
Microbulbifer sp. JAM-3301 BAL70276.1
BAL70274.1
H7CE75  
 endo-inulinase (Inu;IN33) 3.2.1.7 pubmed
Microbulbifer sp. JAM-3301 BAL70278.1
BAL70275.1
H7CE76  
 cycloinulo-oligosaccharide fructanotransferase 2.4.1.- pubmed
Niallia circulans MCI2554 BAA24360.1 O52973  
 levan fructosyltransferase (Lft) 4.2.2.16 pubmed
Paenarthrobacter nicotinovorans GS-9 BAA24915.1 O50585  
 levan fructotransferase (DFA-IV-forming) (LftA) 4.2.2.16 pubmed
Paenarthrobacter ureafaciens K-2032 AAF73829.1
AAX01526.1
AAX01527.1
Q9KJD0 4FFF[A,B,C,D]
4FFG[A,B,C,D]
4FFH[A,B,C,D]
4FFI[A,B,C,D]
 cycloinulo-oligosaccharide fructanotransferase (Cft) 2.4.1.- pubmed
Paenibacillus macerans CFC1 AAG47946.1 Q9F0I5  
 levanase (LelA) 3.2.1.65 pubmed
Paenibacillus polymyxa CF43 CAA81392.1 Q45372  
 cycloinulo-oligosaccharide fructanotransferase (CFTase) 2.4.1.- doi
Paenibacillus polymyxa MG-CF6 BAM74653.1 L8AYK6  
 exo-inulinase (Inu3) 3.2.1.153 pubmed
Paenibacillus polymyxa MGL21 AAL82575.1 Q8RLU2  
 cycloinulo-oligosaccharide fructanotransferase (Cft;CFTase) 2.4.1.- doi
Paenibacillus polymyxa MGL21 AAL82574.1 Q8RLU3  
 exo-β-1,2-fructosidase / fructanase (FruA;BWX39_02900) 3.2.1.153 pubmed
Prevotella intermedia ATCC 25611 = DSM 20706 APW31681.1
BAI39500.1
A0A1P8JD73
C8KIP0
 
 endo-inulinase (Inu1) 3.2.1.7 URL
Pseudomonas mucidolens AAF24999.1 Q9RGC0  
 exo-inulinase (Inu2) 3.2.1.153 URL
Pseudomonas mucidolens AAF44125.1 Q9LAL1  
 invertase (ScrB) 3.2.1.26 pubmed
Salmonella typhimurium CAA47974.1 P37075  
 exo-inulinase (InuA;InuAGN25) 3.2.1.153 doi
Sphingobacterium sp. GN25 AGC01503.1 A0A0E3D7Z1  
 inulinase (InuA;InuAJB13) 3.2.1.153 pubmed
Sphingomonas sp. JB13 AFE82292.1 A0A0F6MTX2  
 invertase (ScrB) 3.2.1.26 pubmed
Staphylococcus xylosus CAA47970.1 Q05936  
 fructan β-fructosidase (FruA) 3.2.1.65
3.2.1.7
pubmed
pubmed
Streptococcus mutans GS-5 AAA26889.1 Q03174  
 invertase (ScrB) 3.2.1.26 pubmed
Streptococcus mutans GS-5 AAA26972.1
CAA35872.1
P13522  
 β-fructosidase (SusH;SP1795;SP_1795) 3.2.1.26 pubmed
Streptococcus pneumoniae TIGR4 AAK75868.1
NP_346228.1
A0A0H2URL9
Q97P49
 
 sucrose-6-P hydrolase (ScrH;SP1724) 3.2.1.26 pubmed
Streptococcus pneumoniae TIGR4 AAK75801.1
NP_346161.1
A0A0H2URG2
Q97PB6
 
 sucrose-6-phosphate hydrolase (ScrB) 3.2.1.26 pubmed
Streptococcus sobrinus S68598    
 β-2,6-fructan 6-levanbiohydrolase (Lf2) 3.2.1.64 pubmed
Streptomyces exfoliatus F3-2 BAC54106.1 Q8GI79  
 invertase (BfrA;Tm1414) 3.2.1.26 pubmed
Thermotoga maritima MSB8 AAD36485.1
AGL50344.1
AHD18692.1
AKE29174.1
CAA04518.1
NP_229215.1
G4FFE9
O33833
1UTW[A,B,C,D,E,F]
1UYP[A,B,C,D,E,F]
1W2T[A,B,C,D,E,F]
 invertase (Inv) 3.2.1.26 doi
uncultured bacterium ACN59531.1 C0LIF8  
 invertase (Inv2;UnInv2) 3.2.1.26 pubmed
uncultured bacterium ADZ31185.1 F1CGX0  
 β-fructofuranosidase (MetaPbio_Contig10a_40--FOS) 3.2.1.26 pubmed
uncultured bacterium CCG34932.1 S6CPR8  
 β-fructofuranosidase (MetaPbio_contig9a_30c--FOS) 3.2.1.26 pubmed
uncultured bacterium CCG34775.1 S6CPG6  
 invertase (ScrB) 3.2.1.26 pubmed
Vibrio alginolyticus AAA27562.1 P13394  
 invertase A (SacA) 3.2.1.26 pubmed
Zymomonas mobilis AAA27701.1 F8DVG5
P22632
P35636
 
 invertase (InvA;ZMO0942) 3.2.1.26 pubmed
Zymomonas mobilis subsp. mobilis ZM4 = ATCC 31821 AAV89566.1
AVZ42197.1
P0DJA7  
 invertase E1 (InvA) 3.2.1.26 pubmed
Zymomonas mobilis Z6C BAA01258.1 P0DJA7
P35636
P35636
 
Eukaryota
Protein Name EC#ReferenceOrganismGenBank UniprotPDB/3D
 sucrose:sucrose 1-fructosyltransferase (Sst2;Atq1-SST2) 2.4.1.99 pubmed
Agave tequilana AFJ21577.1
AFJ21571.1
I1ZBQ5  
 fructan:fructan 6G-fructosyltransferase (Fft1) 2.4.1.243 pubmed
Agave tequilana AFJ21572.1 I1ZBQ0  
 cell wall invertase (Cwinv1;AtqCwinv-1) 3.2.1.26 pubmed
Agave tequilana AFJ21574.1 I1ZBQ2  
 fructan:fructan 6G-fructosyltransferase (Fft1;Atq6G-FFT1) 2.4.1.243 pubmed
Agave tequilana AFJ21578.1 I1ZBQ6  
 cell wall invertase (Cwinv1;AtqCwinv-1) 3.2.1.26 pubmed
Agave tequilana AFJ21580.1 I1ZBQ8  
 sucrose:sucrose 1-fructosyltransferase (Sst1;1-SST) 2.4.1.99 doi
Agave tequilana ABG00265.1 A7RDD3  
 sucrose:sucrose 1-fructosyltransferase 2.4.1.99 pubmed
Allium cepa CAA03099.1    
 invertase (Inv) 3.2.1.26 pubmed
Allium cepa CAA06839.1 O81083  
 sucrose:sucrose 1-fructosyltransferase (SST-1;1-SST) 2.4.1.99 pubmed
Allium cepa CAA06838.1 O81082  
 fructan:fructan 6G-fructosyltransferase (6-G FFT;6G-FFT) 2.4.1.243 pubmed
Allium cepa CAA69170.1 P92916  
 β-fructofuranosidase (SucA) 3.2.1.26 pubmed
Amylomyces rouxii CBS 438.76 BAM95319.1 M1VKR3  
 invertase (AtBFruct4;Fruct4;AtbFruct4;At1g12240) 3.2.1.26 pubmed
Arabidopsis thaliana AAG12569.1
AAK76683.1
AAL65639.1
AAL65640.1
AAL65641.1
AAL65642.1
AAL65643.1
AAL65644.1
AAL65645.1
AAL65646.1
AAL65647.1
AAL65648.1
AAL65649.1
AAL65650.1
AAL65651.1
AAL65652.1
AAL65653.1
AAL65654.1
AAL65655.1
AAL65656.1
AAL65657.1
AAL65658.1
AAL65659.1
AAN13204.1
AEE28855.1
CAA66330.1
CAA72321.1
NP_563901.1
Q39041
Q7DLW9
Q8L3Z1
Q8L488
Q8L4G6
Q8L4T3
Q8L4U9
Q8L514
Q8LGP2
 
 At1g62660 (invertase;AtBFruct3;Fruct3;AtcwINV2) 3.2.1.26 pubmed
Arabidopsis thaliana AAN18078.1
AAD43622.1
AAF19535.1
AAK62665.1
AAK82531.1
AAL32559.1
AAL36260.1
AAM45114.1
AEE33991.1
ANM61060.1
ANM61061.1
ANM61062.1
CAA61624.1
CAA64781.1
CAA67560.1
NP_176453.1
NP_564798.1
A0A1P8AWL7
A0A1P8AWQ1
Q42567
Q43348
Q94BX0
Q9SI83
Q9SXD2
 
 cell-wall invertase 1 (AtBFruct1;Fruct1;AtcwINV1;At3g13790) 3.2.1.26 pubmed
Arabidopsis thaliana AAK76450.1
AAL85153.1
AEE75414.1
AEE75415.1
ANM65335.1
ANM65336.1
ANM65337.1
BAB01930.1
BAH56786.1
CAA52619.1
CAA52620.1
NP_187995.1
NP_566464.1
A0A1I9LRX7
A0A5S9XBW4
C0Z257
F4JEJ0
Q43866
Q9LIB8
2AC1[A]
2OXB[A]
2QQU[A]
2QQV[A]
2QQW[A]
2XQR[A,C,E,G,I,K]
 invertase 4 (Atfruct6;AtcwINV4;At2g36190) 3.2.1.26 pubmed
Arabidopsis thaliana AAD21446.2
AAM15406.1
AAM61359.1
AEC09214.1
BAB83031.1
NP_565837.1
Q8L5V2
Q8W413
 
 fructan 1-exohydrolase (AlEH2) 3.2.1.153 doi
Arctium lappa BAR88557.1 A0A0H5AH66  
 fructan:fructan 1-fructosyltransferase (AlFt1;1-FEH) 2.4.1.100 doi
Arctium lappa BAJ24841.1 E2RV71  
 fructan 1-exohydrolase (AlEH1;1-FEH) 3.2.1.153 pubmed
Arctium lappa BAL73222.1 I0FXI5  
 fructan:fructan 6G-fructosyltransferase (AoFT1;6-G FFT;6G-FFT) 2.4.1.243 pubmed
Asparagus officinalis BAD89564.1 Q5FC15  
 exo-inulinase (Inu1) 3.2.1.153 pubmed
Aspergillus awamori var. 2250 CAC44220.1 Q96TU3 1Y4W[A]
1Y9G[A]
1Y9M[A]
 endo-inulinase (Inu2) 3.2.1.7 pubmed
Aspergillus ficuum ATCC 16882 CAA07345.1
ACR55693.1
C5J3N4
O94220
3RWK[X]
3SC7[X]
 sucrose:sucrose 1-fructosyltransferase (1-Sst) 2.4.1.99 pubmed
Aspergillus foetidus CAA04131.1 O42801  
 β-fructofuranosidase (FopA) 3.2.1.26 pubmed
Aspergillus japonicus ATCC 20611 BAB67771.1 Q96VC5  
 fructosyltransferase (FT) 2.4.1.100 pubmed
Aspergillus japonicus CB05 ADK46938.1 F1ADK9
Q1W3Z7
3LDK[A]
3LDR[A]
3LEM[A]
3LF7[A]
3LFI[A,B]
3LIG[A]
3LIH[A]
 fructosyltransferase (AKAW_06215) 2.4.1.100 pubmed
Aspergillus luchuensis IFO 4308 GAA88101.1 G7XM46 5XH8[A]
5XH9[A]
5XHA[A]
 exo-inulinase (InuE) 3.2.1.153 pubmed
Aspergillus niger 12 BAD01476.1 Q76HP6 cryst
 endo-inulinase A (InuA) 3.2.1.7 pubmed
Aspergillus niger 12 BAA33797.1 O74641  
 endo-inulinase B (InuB) 3.2.1.7 pubmed
Aspergillus niger 12 BAA33798.1 O74642  
 endoinulinase (EndoInu) 3.2.1.7 URL
Aspergillus niger 9891 AAN64131.1
AAO18341.1
Q874R2
Q8J229
 
 inulinase (InuA1) 3.2.1.7 doi
Aspergillus niger AF10 AAK43726.1 O74641
Q96WZ8
 
 β-fructofuranosidase (Suc1) 3.2.1.26 pubmed
Aspergillus niger B60 S33920    
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