Glycoside Hydrolase Family 32

Activities in Familyinvertase (EC 3.2.1.26); endo-inulinase (EC 3.2.1.7); β-2,6-fructan 6-levanbiohydrolase (EC 3.2.1.64); endo-levanase (EC 3.2.1.65); exo-inulinase (EC 3.2.1.80); fructan β-(2,1)-fructosidase/1-exohydrolase (EC 3.2.1.153); fructan β-(2,6)-fructosidase/6-exohydrolase (EC 3.2.1.154); sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99); fructan:fructan 1-fructosyltransferase (EC 2.4.1.100); sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10); fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243); levan fructosyltransferase (EC 2.4.1.-); [retaining] sucrose:sucrose 6-fructosyltransferase (6-SST) (EC 2.4.1.-); cycloinulo-oligosaccharide fructanotransferase (EC 2.4.1.-)
Mechanism Retaining
ClanGH-J
3D Structure Status5-fold β-propeller
Catalytic Nucleophile/BaseAsp
Catalytic Proton DonorGlu
External resourcesCAZypedia; PROSITE;
Commercial Enzyme Provider(s)MEGAZYME;
Statistics GenBank accession (7286); Uniprot accession (1294); PDB accession (49); 3D entries (14); cryst (2)
All (6640) Archaea (16) Bacteria (5425) Eukaryota (1197) Viruses (2) Structure (14 - 2 cryst) Characterized (252)
Bacteria
Protein Name EC#OrganismGenBankUniprot
PDB/3D Carbohydrate Ligands Resolution (Å)
 β-fructofuranosidase 3.2.1.26 Bifidobacterium longum KN29.1 ABN04092.1 A2TLS9
3PIG[A,B] 1.87
3PIJ[A,B] β-D-Fruf
1.80
 exo-inulinase (InuA) 3.2.1.153 Geobacillus stearothermophilus KP1289 BAC45010.1 Q8GI55
cryst    
 levan fructotransferase (DFA-IV-forming) (LftA) 4.2.2.16 Paenarthrobacter ureafaciens K-2032 AAF73829.1
AAX01526.1
AAX01527.1
Q9KJD0
4FFF[A,B,C,D] 2.57
4FFG[A,B,C,D] β-D-Fruf-(2-6)-β-D-Fruf
<carb_like_0U8>
2.30
4FFH[A,B,C,D] α-D-Glcp-(1-2)-β-D-Fruf
2.20
4FFI[A,B,C,D] β-D-Fruf-(2-6)-β-D-Fruf
β-D-Fruf-(2-6)-β-D-Fruf-(2-6)-β-D-Fruf
2.30
 invertase (BfrA;Tm1414) 3.2.1.26 Thermotoga maritima MSB8 AAD36485.1
AGL50344.1
AHD18692.1
AKE29174.1
CAA04518.1
NP_229215.1
G4FFE9
O33833
1UTW[A,B,C,D,E,F] 1.90
1UYP[A,B,C,D,E,F] 1.9
1W2T[A,B,C,D,E,F] α-D-Galp-(1-6)-α-D-Glcp-(1-2)-β-D-Fruf
1.87
Eukaryota
Protein Name EC#OrganismGenBankUniprot
PDB/3D Carbohydrate Ligands Resolution (Å)
 cell-wall invertase 1 (AtBFruct1;Fruct1;AtcwINV1;At3g13790) 3.2.1.26 Arabidopsis thaliana AAK76450.1
AAL85153.1
AEE75414.1
AEE75415.1
ANM65335.1
ANM65336.1
ANM65337.1
BAB01930.1
BAH56786.1
CAA52619.1
CAA52620.1
NP_187995.1
NP_566464.1
Q43866
Q9LIB8
2AC1[A] 2.15
2OXB[A] α-D-Glcp-(1-2)-β-D-Fruf
2.60
2QQU[A] α-D-Glcp-(1-2)-β-D-Fruf
2.84
2QQV[A] α-D-Glcp-(1-2)-β-D-Fruf
3.01
2QQW[A] α-D-Glcp-(1-2)-β-D-Fruf
2.80
2XQR[A,C,E,G,I,K] β-D-Fruf
2.58
 exo-inulinase (Inu1) 3.2.1.80 Aspergillus awamori var. 2250 CAC44220.1 Q96TU3
1Y4W[A] 1.55
1Y9G[A] β-D-Fruf
1.87
1Y9M[A] D-1-deoxy-Manp
1.89
 endo-inulinase (Inu2) 3.2.1.7 Aspergillus ficuum ATCC 16882 CAA07345.1
ACR55693.1
O94220
3RWK[X] α-D-Manp-(1-3)-α-D-Manp-(1-6)-D-1-deoxy-Manp
β-D-Fruf
2.10
3SC7[X] α-D-Manp-(1-3)-α-D-Manp-(1-6)-D-1-deoxy-Manp
1.50
 fructosyltransferase (FT) 2.4.1.100 Aspergillus japonicus CB05 ADK46938.1  
3LDK[A] α-D-Glcp-(1-2)-β-D-Fruf
2.20
3LDR[A] α-D-Glcp-(1-2)-β-D-Fruf-(1-2)-β-D-Fruf
2.10
3LEM[A] α-D-Glcp-(1-2)-β-D-Fruf-(1-2)-β-D-Fruf-(1-2)-β-D-Fruf
2.10
3LF7[A] 2.00
3LFI[A,B] β-D-Glcp
2.00
3LIG[A] 1.80
3LIH[A] α-D-Galp-(1-6)-α-D-Glcp-(1-2)-β-D-Fruf
2.20
 exo-inulinase (InuE) 3.2.1.80 Aspergillus niger 12 BAD01476.1 Q76HP6
cryst    
 fructan b-(2,1)-fructosidase; fructan 1-exohydrolase IIa (1-FEH IIa) 3.2.1.153 Cichorium intybus CAC37922.1
AAP85536.1
AFU54056.1
Q93X60
1ST8[A] 2.35
2ADD[A] α-D-Glcp-(1-2)-β-D-Fruf
2.50
2ADE[A] β-D-Fruf
2.65
2AEY[A] <carb_like_DQQ>
3.27
2AEZ[A] α-D-Glcp-(1-2)-β-D-Fruf-(1-2)-β-D-Fruf
3.05
 sucrose:{sucrose/fructan} 6-fructosyltransferase (6-Sst/6-Sft) 2.4.1.10
2.4.1.-
Pachysandra terminalis CBM41476.2
CBM41476.1
E3PQS3
3UGF[A,B] 1.70
3UGG[A,B] α-D-Glcp-(1-2)-β-D-Fruf-(1-2)-β-D-Fruf
2.90
3UGH[A,B] 2.90
 invertase 2 (Suc2;YIL162w) 3.2.1.26 Saccharomyces cerevisiae S288c CAA24618.1
BAJ07819.1
CAA87030.1
NP_012104.1
D5MS22
P00724
4EQV[A,B,C,D,E,F,G,H] 3.40
 invertase / β-fructofuranosidase (Inv;SoINV;Ffase) 3.2.1.26 Schwanniomyces occidentalis ATCC 26077 ADN34605.1
AAE51668.1
CAA35606.1
E5D0X5
P24133
3KF3[A,B] β-D-Fruf
1.90
3KF5[A,B] 2.90
3U14[A,B] β-D-Fruf-(2-1)-<non_carb>
2.24
3U75[A,B,C,D] α-D-Glcp-(1-2)-β-D-Fruf
2.68
 β-fructofuranosidase (Inv;Xd-INV;XdINV) 3.2.1.26 Xanthophyllomyces dendrorhous ATCC MYA-131 ACL79833.1 B8YJM2
5ANN[A,B] 2.14
5FIX[A,B] α-D-Glcp-(1-2)-β-D-Fruf
2.01
5FK7[A,B] 2.05
5FK8[A,B] β-D-Fruf-(2-6)-α-D-Glcp-(1-4)-α-D-Glcp
1.88
5FKB[A,B] α-D-Glcp-(1-2)-β-D-Fruf
1.78
5FKC[A,B] 1.82
5FMB[A,B] β-D-Fruf
1.91
5FMC[A,B] β-D-Fruf
1.84
5FMD[A,B] D-1-deoxy-Manp
α-D-Glcp-(1-2)-β-D-Fruf-(1-2)-β-D-Fruf-(1-2)-β-D-Fruf
1.78
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