Glycoside Hydrolase Family 29

Activities in Family	α-L-fucosidase (EC 3.2.1.51); α-1,3/1,4-L-fucosidase (EC 3.2.1.111); α-1,2-L-fucosidase (EC 3.2.1.63); α-1,6-fucosidase (EC 3.2.1.127)
Mechanism	Retaining
Clan	GH-R
3D Structure Status	( β / α ) ₈ barrel
Catalytic Nucleophile/Base	Asp (experimental)
Catalytic Proton Donor	Glu (experimental)
External resources	CAZypedia; PROSITE; SMART;
Commercial Enzyme Provider(s)	MEGAZYME;
Statistics	GenBank accession (8556); Uniprot accession (54); PDB accession (66); 3D entries (14); cryst (0)

Summary

Download GH29 (8313) Taxonomic display Structure (14) Characterized (47)

Bacteria

Protein Name

EC#

Organism

GenBank

Uniprot

PDB/3D

Carbohydrate Ligands

Resolution (Å)

Bovatus_01698

Bacteroides ovatus ATCC 8483

ALJ46339.1

4ZRX[A]

1.59

α-1,3/1,4-L-fucosidase / α-galactosidase (BT2192;BT_2192)

3.2.1.111

Bacteroides thetaiotaomicron VPI-5482

AAO77299.1
NP_811105.1

Q8A5P6

3EYP[A,B]		1.90
4OUE[A,B]	β-D-Galp1S-(1-1)-<non_carb>	2.35
4OZO[A,B]	β-D-Galp-(1-1)-<non_carb>	2.60

α-L-fucosidase (Fuc2970;BtFuc2970;BT2970;BT_2970)

3.2.1.51

Bacteroides thetaiotaomicron VPI-5482

AAO78076.1
NP_811882.1

Q8A3I4

2WVS[A,B,C,D]	β-L-Fucp2F β-L-Fucp2F-(1-4)-ASP	2.19
2WVT[A,B]	<carb_like_FHN>	1.80
2WVU[A,B,C,D]	α-L-Fucp-(1-1)-<non_carb>	1.95
2WVV[A,B,C,D]		1.73
2XIB[A,B,C,D]	<carb_like_DFU>	2.20
2XII[A,B]	<carb_like_TA9>	1.80
4J27[A,B]		1.59
4J28[A,B]	<carb_like_EAT>	1.73
4JFS[A,B]	<carb_like_16Z>	2.00
4JFT[A,B]	<carb_like_1KN>	2.10
4JFU[A,B]	<carb_like_K80>	1.66
4JFV[A,B,C,D]	<carb_like_H57>	1.88
4JFW[A,B,C,D]	<carb_like_H58>	2.10
4JL1[A,B]	<carb_like_LM5>	1.68
4JL2[A,B]	<carb_like_LM5>	1.70
4PCS[A,B,C,D]	<carb_like_2M7>	1.77
4PCT[A,B,C,D]	<carb_like_H76>	2.10
4PEE[A,B,C,D]	<carb_like_2OX>	1.95
4WSJ[A,B,C,D]	<carb_like_3U3>	1.64
4WSK[A,B,C,D]	<carb_like_3U2>	1.92
5HDR[A,B,C,D]		2.30
5I5R[A,B,C,D]		2.10
6HZY[A,B]	α-L-Fucp1N-(1-1)-<non_carb> β-L-Fucp1N-(1-1)-<non_carb>	1.70

BT3798

Bacteroides thetaiotaomicron VPI-5482

AAO78903.1
NP_812709.1

3GZA[A,B]

1.60

α-1,3/1,4-L-fucosidase (BiAfcB;Blon_2336)

3.2.1.51
3.2.1.111

Bifidobacterium longum subsp. infantis ATCC 15697 = JCM 1222 = DSM 20088

ACJ53394.1

B7GNN8

3MO4[A,B]		1.90
3UES[A,B]	<carb_like_DFU>	1.60
3UET[A,B]	α-L-Fucp-(1-4)[β-D-Galp-(1-3)]-β-D-GlcpNAc	2.10

BN194_28780

Lacticaseibacillus casei W56

CCK23825.1

6O18[A,B,C,D]		2.55
6O1A[A,B,C,D]	β-L-Fucp	2.60
6O1C[A,B,C,D,E,F,G,H]	α-L-Fucp-(1-1)-<non_carb>	2.60
6O1I[A,B,C,D]		3.55
6O1J[A,B,C,D,E,F,G,H]	β-L-Fucp	2.00
6OHE[A,B,C,D]	α-L-Fucp-(1-6)-β-D-GlcpNAc	3.14

α-L-fucosidase (aLfuk1)

3.2.1.51

Paenibacillus thiaminolyticus

CBM40947.1

E3PQQ9

6GN6[A,B,C,D,E,F]

α-D-Glcp
α-D-Glcp-(1-4)-α-D-Glcp

2.20

α-1,3-galactosidase active on porphyrooligosaccharides desulfated at the non-reducing end (BpGH29; BACPLE_01702)

3.2.1.-

Phocaeicola plebeius DSM 17135

EDY95436.1

7LJJ[A]		1.90
7LK7[A]	α-L-Galp	2.19
7LNP[A]	α-L-Galp-(1-1)-<non_carb>	2.70

CDL26_02305

3.2.1.111

Ruminococcus gnavus RJX1124

PLT74914.1

A0A2N5PIE7

6TR3[A]	β-L-Fucp	1.70
6TR4[A]	α-L-Fucp	1.45

α-1,3/1,4-L-fucosidase (SpGH29;SP_2146)

3.2.1.111

Streptococcus pneumoniae TIGR4

AAK76203.1
ABA35887.1
NP_346563.1

A0A0H2US78
Q97NA6

6OR4[A,B]	α-L-Fucp-(1-4)[β-D-Galp-(1-3)]-β-D-GlcpNAc	2.10
6ORF[A,B]	β-D-Galp-(1-4)[α-L-Fucp-(1-3)]-α-D-GlcpNAc	1.70
6ORG[A,B]		1.72
6ORH[A,B]	α-L-Fucp-(1-2)-β-D-Galp-(1-4)[α-L-Fucp-(1-3)]-α-D-GlcpNAc	1.62

α-L-fucosidase (TmaFuc;TM0306;Tmari_0304)

3.2.1.51

Thermotoga maritima MSB8

AAD35394.1
AGL49230.1
AHD17930.1
AKE28107.1
NP_228118.1

G4FHM0
Q9WYE2

1HL8[A,B]		2.4
1HL9[A,B]	β-L-Fucp2F-(1-4)-ASP	2.25
1ODU[A,B]	β-L-Fucp	2.8
2WSP[A,B]	α-L-Fucp-(1-2)-β-L-Fucp1N-(1-1)-<non_carb>	2.65
2ZWY[A,B]		2.75
2ZWZ[A,B]	<carb_like_ZWZ>	2.36
2ZX5[A,B]	<carb_like_ZX5>	2.65
2ZX6[A,B]	<carb_like_ZX6>	2.42
2ZX7[A,B]	<carb_like_ZX7>	2.48
2ZX8[A,B]	<carb_like_ZX8>	2.33
2ZX9[A,B]	<carb_like_ZX9>	2.62
2ZXA[A,B]	<carb_like_ZXA>	2.57
2ZXB[A,B]	<carb_like_ZXB>	2.61
2ZXD[A,B]	<carb_like_ZXD>	2.15