Glycoside Hydrolase Family 28

Activities in Familypolygalacturonase (EC 3.2.1.15); exo-polygalacturonase (EC 3.2.1.67); exo-polygalacturonosidase (EC 3.2.1.82); rhamnogalacturonase (EC 3.2.1.171); rhamnogalacturonan α-1,2-galacturonohydrolase (EC 3.2.1.173); xylogalacturonan hydrolase (EC 3.2.1.-)
Mechanism Inverting
ClanGH-N
3D Structure Statusparallel β-helix
Catalytic Nucleophile/BaseAsp
Catalytic Proton DonorAsp
External resourcesCAZypedia; HOMSTRAD; PROSITE;
Commercial Enzyme Provider(s)MEGAZYME;
Statistics GenBank accession (11536); Uniprot accession (1123); PDB accession (20); 3D entries (18); cryst (3)
All (9622) Archaea (17) Bacteria (5290) Eukaryota (4309) unclassified (6) Structure (18 - 3 cryst) Characterized (216)
Bacteria
Protein Name EC#OrganismGenBankUniprot
PDB/3D Carbohydrate Ligands Resolution (Å)
 polygalacturonase (PehA) 3.2.1.15 Agrobacterium vitis CG49 AAB51699.1 P77818
1ZFW[nd]
 homogalacturonanan specific α-galacturonidase / exo-polygalacturonase (BT4155;BT_4155) 3.2.1.67
3.2.1.-
Bacteroides thetaiotaomicron VPI-5482 AAO79260.1
NP_813066.1
Q8A066
5OLP[A,B] 1.90
 PARMER_00599 (fragment)   Parabacteroides merdae ATCC 43184 EDN88021.1  
4MXN[A,B,C,D] 1.20
 polygalacturonase-2 (PehA) 3.2.1.15 Pectobacterium carotovorum SCC3193 CAA35998.1 P26509
1BHE[A] 2.30
 exo-polygalacturonase (GalU;PelB;ExoPG;TM0437;TM_0437;Tmari_0434) 3.2.1.67 Thermotoga maritima MSB8 AAD35522.1
AGL49359.1
AHD17805.1
AKE28230.1
NP_228247.1
Q9WYR8
3JUR[A,B,C,D] 1.80
 exo-polygalacturonosidase (PehX) (YeGH28) 3.2.1.82 Yersinia enterocolitica ATCC 9610D AAC15064.1 O68975
2UVE[A,B] 1.31
2UVF[A,B] α-D-GalpA-(1-4)-α-D-GalpA
1.80
Eukaryota
Protein Name EC#OrganismGenBankUniprot
PDB/3D Carbohydrate Ligands Resolution (Å)
 polygalacturonase (Pga1) 3.2.1.15 Aspergillus aculeatus KSM 510 AAC23565.1 O74213
1IA5[A] 1.91
1IB4[A,B] 2.15
 rhamnogalacturonase A (RhgA) 3.2.1.171 Aspergillus aculeatus KSM 510 / CBS 115.80 AAA64367.1
AAB35485.1
CAA58507.2
CAB58937.1
CAB58938.1
Q00001
1RMG[A] 1.62
 endopolygalacturonase-1 / I (PgaI;PgaA;AnPGI) 3.2.1.15 Aspergillus niger CBS 120.49 / N400 CAA41693.1 P26213
1NHC[A,B,C,D,E,F] 1.20
 endopolygalacturonase-2 / II (PgaII;PgaB;AnPGII) 3.2.1.15 Aspergillus niger CBS 120.49 / N400 CAA41694.1 P26214
1CZF[A,B] 1.90
 xylogalacturonan hydrolase (XghA) 3.2.1.- Aspergillus tubingensis CAB65657.1
CAC07733.1
Q9UUZ2
4C2L[A] 0.96
 endopolygalacturonase I (Pg1;endoPG I) 3.2.1.15 Chondrostereum purpureum BAA08102.1
BAA96351.1
P79074
1K5C[A] 2.50
1KCC[A] β-D-GalpA
2.20
1KCD[A] β-D-GalfA
β-D-GalpA
3.01
 endopolygalacturonase (Pg1) 3.2.1.15 Colletotrichum lupini var. setosum (nom. inval.) SHK788 ABL01533.1 A1E266
2IQ7[A,B,C,D,E,F,G] 1.45
 endo-polygalacturonase (TlpgA)   Evansstolkia leycettana JCM 12802 QKK82827.1  
6KVE[A] 1.76
6KVH[A] 2.21
 polygalacturonase (PgA;PgI;FmPG) 3.2.1.15 Fusarium verticillioides FC-10 AAA74586.1 Q07181
1HG8[A] NMR
 endopolygalacturonase (PPSX1;SX1) 3.2.1.15 Geotrichum klebahnii ATCC 42397 AAE82584.1  
cryst    
 polygalacturonase (PSE3;SE3) 3.2.1.15 Geotrichum klebahnii B2 BAA20427.1
AAB46926.1
P87217
cryst    
 polygalacturonase 2 3.2.1.15 Solanum lycopersicum AAB09575.1
AAC28904.1
CAX05861.1
CBD29102.1
Q96487
cryst    
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