Glycoside Hydrolase Family 20

Activities in Familyβ-hexosaminidase (EC 3.2.1.52); lacto-N-biosidase (EC 3.2.1.140); β-1,6-N-acetylglucosaminidase) (EC 3.2.1.-); β-6-SO3-N-acetylglucosaminidase (EC 3.2.1.-)
Mechanism Retaining
ClanGH-K
3D Structure Status( β / α ) 8
Catalytic Nucleophile/Basecarbonyl oxygen of C-2 acetamido group of substrate
Catalytic Proton DonorGlu
External resourcesCAZypedia; HOMSTRAD; PRINTS; Tay-Sachs Disease;
Commercial Enzyme Provider(s)MEGAZYME;
Statistics GenBank accession (4615); Uniprot accession (779); PDB accession (79); 3D entries (18); cryst (1)
All (4333) Archaea (4) Bacteria (4008) Eukaryota (310) Viruses (1) unclassified (10) Structure (18 - 1 cryst) Characterized (124)
Bacteria
Protein Name EC#OrganismGenBankUniprot
PDB/3D Carbohydrate Ligands Resolution (Å)
 dispersin B / β-1,6-N-acetylglucosaminidase (DspB) 3.2.1.- Aggregatibacter actinomycetemcomitans CU1000 AAP31025.1 Q840G9
1YHT[A] 2.00
 β-N-acetylhexosaminidase (BF3095;BF9343_3009)   Bacteroides fragilis NCTC 9343 CAH08790.1 Q5LAT3
4PYS[A,B] 1.82
 lacto-N-biosidase (LnbB) 3.2.1.140 Bifidobacterium bifidum JCM 1254 ABZ78855.1 B3TLD6
4H04[A,B] β-D-Galp-(1-3)-β-D-GlcpNAc
1.80
4JAW[A,B] 1.80
5BXP[A,B] β-D-Galp-(1-3)-α-D-Glcp1N2NAc-(1-1)-<non_carb>
β-D-Galp-(1-3)-β-D-Glcp1N2NAc-(1-1)-<non_carb>
1.70
5BXR[A,B] β-D-Galp-(1-1)-<non_carb>
<carb_like_NOK>
1.60
5BXS[A,B] β-D-Galp-(1-1)-<non_carb>
<carb_like_GC2>
2.20
5BXT[A,B] β-D-Galp-(1-1)-<non_carb>
1.80
 β-N-acetylhexosaminidase (AAur_4089)   Paenarthrobacter aurescens TC1 ABM09106.1 A1RBZ5
3RCN[A] 2.51
 β-hexosaminidase (Hex1T) 3.2.1.52 Paenibacillus sp. TS12 BAI63641.1 D2KW09
3GH4[A] 1.80
3GH5[A] β-D-GlcpNAc
1.60
3GH7[A] β-D-GalpNAc
1.90
3SUR[A] 1.90
3SUS[A] D-1,2-deoxy-Galp
1.80
3SUT[A] α-D-Glcp1N2NAc-(1-1)-<non_carb>
1.90
3SUU[A] α-D-Galp1N2NAc-(1-1)-<non_carb>
1.60
3SUV[A] <carb_like_NOK>
1.60
3SUW[A] <carb_like_GC2>
1.90
 chitobiase (Chb;Ctb;SmCHB;SmGH20A) 3.2.1.52 Serratia marcescens 2170 AAB03808.1 Q54468
1C7S[A] β-D-GlcpNAc-(1-4)-β-D-GlcpNAc
1.80
1C7T[A] β-D-GlcpNAc-(1-4)-β-D-GlcpNAc
1.90
1QBA[A] 1.85
1QBB[A] β-D-GlcpNAc-(1-4)-α-D-GlcpNAc
2.00
 β-N-acetylglucosaminidase (GcnA) 3.2.1.52 Streptococcus gordonii FSS2 AAS02154.1 Q6ST21
2EPK[X] 1.85
2EPL[X] 1.40
2EPM[X] 2.04
2EPN[A,B] 1.61
2EPO[A,B] 1.56
 β-N-acetylhexosaminidase (StrH;spr0057) 3.2.1.52 Streptococcus pneumoniae R6 AAK98861.1
NP_357651.1
Q8DRL6
3RPM[A] β-D-GlcpNAc
2.10
 β-N-acetylhexosaminidase (StrH;spr0057) 3.2.1.52 Streptococcus pneumoniae R6 AAK98861.1
NP_357651.1
Q8DRL6
3RPM[A,B] β-D-GlcpNAc
2.10
 β-N-acetylhexosaminidase (GH20C;SP2141;SP_2141) 3.2.1.52 Streptococcus pneumoniae TIGR4 AAK76198.1  
5A69[A] β-D-Galp1N2NAc-(1-1)-<non_carb>
2.20
5A6A[A,B] 2.69
5A6B[A,B,C,D] β-D-Glcp1N2NAc-(1-1)-<non_carb>
1.77
5A6J[A,B,C,D] 1.94
5A6K[A,B] D-1,2-deoxy-Galp
2.10
5AC4[A,B] β-D-GalpNAc
2.08
5AC5[A,B] β-D-GlcpNAc
1.84
 β-N-acetylglucosaminidase (StrH;SP0057;SP_0057) 3.2.1.52 Streptococcus pneumoniae TIGR4 AAK74246.1
NP_344606.1
P49610
2YL6[A] 1.60
2YL8[A] β-D-GlcpNAc-(1-2)-α-D-Manp
1.75
2YLL[A] 1.85
4AZ5[A] 1.73
4AZ6[A] α-D-Glcp1N2NAc-(1-1)-<non_carb>
1.36
4AZ7[A] β-D-Glcp1N2NAc-(1-1)-<non_carb>
1.70
4AZB[A] α-D-Glcp1N2NAc-(1-1)-<non_carb>
2.10
 β-N-acetylglucosaminidase (StrH;SP0057;SP_0057) 3.2.1.52 Streptococcus pneumoniae TIGR4 AAK74246.1
NP_344606.1
P49610
2YL5[A,B,C,D] 2.15
2YL9[A,B,C,D] β-D-GlcpNAc-(1-2)-α-D-Manp-(1-3)-β-D-Manp-(1-4)-β-D-GlcpNAc
2.65
2YLA[A,B,C,D] β-D-GlcpNAc-(1-2)-α-D-Manp-(1-3)-β-D-Manp-(1-4)-β-D-GlcpNAc
β-D-GlcpNAc-(1-2)-α-D-Manp-(1-3)[β-D-GlcpNAc-(1-4)]-β-D-Manp-(1-4)-β-D-GlcpNAc
2.70
4AZC[A,B,C,D] 2.09
4AZG[A,B] α-D-Glcp1N2NAc-(1-1)-<non_carb>
β-D-Glcp1N2NAc-(1-1)-<non_carb>
2.40
4AZH[A,B,C,D] β-D-Glcp1N2NAc-(1-1)-<non_carb>
2.22
4AZI[A,B] α-D-Glcp1N2NAc-(1-1)-<non_carb>
β-D-Glcp1N2NAc-(1-1)-<non_carb>
1.98
 β-N-acetylhexosaminidase (HexA;SCO2786) 3.2.1.52 Streptomyces coelicolor A3(2) CAB87223.1
NP_627016.1
Q9L068
4C7D[A,B] 1.85
4C7F[A,B] <carb_like_GC2>
2.00
4C7G[A] 1.80
 β-N-acetylglucosaminidase (Hex;SpHex) 3.2.1.52 Streptomyces plicatus AAC38798.3 O85361
1HP4[A] 2.20
1HP5[A] 2.10
1JAK[A] <carb_like_IFG>
1.75
1M01[A] β-D-GlcpNAc
2.10
1M03[A] β-D-GlcpNAc
1.90
1M04[A] β-D-GlcpNAc
1.95
5FCZ[A] <non_carb>-(1-2)-D-1-deoxy-GlcpN
2.45
5FD0[A] β-D-GlcpNAc
2.00
Eukaryota
Protein Name EC#OrganismGenBankUniprot
PDB/3D Carbohydrate Ligands Resolution (Å)
 β-N-acetylhexosaminidase (HexA) 3.2.1.52 Aspergillus oryzae CCF 1066 AAM13977.1 Q8J2T0
cryst    
 β-N-acetylglucosaminidase / β-hexosaminidase A (α-chain) (HexA) 3.2.1.52 Homo sapiens AAB00965.1
AAA51827.1
AAD13932.1
AAH01138.2
AAH18927.1
AAH21030.1
AAH84537.1
AAQ70000.1
ACM85216.1
ALQ33567.1
ALQ33569.1
ALQ33570.1
BAD96222.1
BAG59159.1
BAG60039.1
BAG62619.1
BAG62730.1
CAH10482.1
NP_000511.1
P06865
Q9BVJ8
2GJX[A,D,E,H] 2.80
2GK1[A,C,E,G] 3.25
 β-N-acetylglucosaminidase B (β-chain) (HexB) 3.2.1.52 Homo sapiens AAA52645.1
AAA51828.1
AAA51829.1
AAA68620.1
AAH17378.1
AAM46114.1
AAP88921.1
AAV48875.1
ACI46028.1
ACV87197.1
NP_000512.1
P07686
1NOU[A,B] 2.40
1NOW[A,B] <carb_like_IFG>
2.20
1NP0[A,B] 2.50
1O7A[A,B,C,D,E,F] α-D-GlcpNAc
β-D-GlcpNAc
2.25
2GJX[B,C,F,G] 2.80
2GK1[B,D,F,H] 3.25
3LMY[A,B] 2.80
5BRO[A] 2.40
 β-N-acetylhexosaminidase (Hex1;OfHex1) 3.2.1.52 Ostrinia furnacalis ABI81756.1 Q06GJ0
3NSM[A] 2.10
3NSN[A] β-D-Glcp2NMe-(1-4)-β-D-GlcpNAc-(1-4)-β-D-GlcpNAc-(1-4)-β-D-GlcpNAc
2.10
3OZO[A] 2.00
3OZP[A] α-D-Glcp1N2NAc-(1-1)-<non_carb>
2.00
3S6T[A] β-D-Glcp1N2NAc-(1-1)-<non_carb>
2.30
3VTR[A] D-1-deoxy-GlcpNAc
β-D-Glcp2NMe-(1-4)-β-D-GlcpNAc-(1-4)-β-D-GlcpNAc-(1-4)-β-D-GlcpNAc
2.50
3WMB[A] 2.70
3WMC[A] 2.10
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