Glycoside Hydrolase Family 16

Known Activities	xyloglucan:xyloglucosyltransferase (EC 2.4.1.207); keratan-sulfate endo-1,4-β-galactosidase (EC 3.2.1.103); endo-1,3-β-glucanase (EC 3.2.1.39); endo-1,3(4)-β-glucanase (EC 3.2.1.6); licheninase (EC 3.2.1.73); β-agarase (EC 3.2.1.81); κ-carrageenase (EC 3.2.1.83); xyloglucanase (EC 3.2.1.151); endo-β-1,3-galactanase (EC 3.2.1.-); β-porphyranase (EC 3.2.1.178)
Mechanism	Retaining
Clan	GH-B
3D Structure Status	β-jelly roll
Catalytic Nucleophile/Base	Glu (experimental)
Catalytic Proton Donor	Glu (experimental)
External resources	CAZypedia; GHDB; XTH-World; HOMSTRAD; InterPro; PFAM; PRINTS; PROSITE;
Commercial Enzyme Provider(s)	MEGAZYME; NZYTech; PROZOMIX;
Statistics	GenBank accession (2845); Uniprot accession (1414); PDB accession (47); 3D entries (23); cryst (2)

Summary

All (2043) Archaea (5) Bacteria (845) Eukaryota (1179) Viruses (7) unclassified (7) Structure (23 - 2 cryst) Characterized (167)

Archaea
Protein Name	EC#	Organism	GenBank	Uniprot	PDB/3D
endo-β-1,3-glucanase (LamA;PF0076) (Lam16)	3.2.1.39	Pyrococcus furiosus DSM 3638	AAC25554.2 AAL80200.1 NP_577805.1	O73951	2VY0[A,B]
Bacteria
Protein Name	EC#	Organism	GenBank	Uniprot	PDB/3D
endo-β-1,3-glucanase (peptide fragment)	3.2.1.39	Arthrobacter sp.			cryst
lichenase / β-1,3-1,4-glucanase (Bg1)	3.2.1.73	Bacillus licheniformis	CAA40547.1	P27051.1	1GBG[A] 3D6E[A,B]
lichenase (BglS;LicS;Bgl;BSU39070)	3.2.1.73	Bacillus subtilis subsp. subtilis str. 168	AAM08358.1 CAA25328.1 CAA86922.1 CAB15943.1 CAJ15097.1 NP_391786.1	P04957.2	3O5S[A]
β-1,3-glucanase II (BglII)	3.2.1.39	Cellulosimicrobium cellulans LL-G109 / DSM10297 / DK-1	AAB39377.1 AAB39378.1 AAB47151.1 AAB47152.1 AAB47153.1 AAC38290.1 AEK80404.1	O68641	3ATG[A]
GlcNAc-α-1,4-Gal-releasing endo-β-galactosidase (GngC)	3.2.1.-	Clostridium perfringens ATCC 10543	BAB69460.1	Q934G8	1UPS[A,B]
lichenase / endo-β-1,3-1,4-glucanase (TF-glucanase;Fisuc_2961;FSU_0226)	3.2.1.73	Fibrobacter succinogenes subsp. succinogenes S85	AAA24896.1 AAA24896.1 ACR92625.1 ACR92625.1 ACR92630.1 ACR92630.1 ACR92631.1 ACR92631.1 ACR92632.1 ACR92632.1 ACR92633.1 ACR92633.1 ACR92636.1 ACR92636.1 ACR92637.1 ACR92637.1 ACX76541.1 ACX76541.1 ADL24776.1 ADL24776.1	P17989.1 P17989.1	1MVE[A,A] 1ZM1[A,A,B,B] 2R49[A,A] 2R4A[A,A] 3H0O[A,A] 3HR9[A,A]
MSMEG_5345		Mycobacterium smegmatis str. MC2 155	ABK72351.1	A0R351	3RQ0[A]
β-1,3-glucanase F (BglF)	3.2.1.39	Nocardiopsis sp. F96	BAE54302.1	Q2V0H0	2HYK[A]
lichenase M (Bgi)	3.2.1.73	Paenibacillus macerans	AAO66468.1 CAA39426.1	P23904.2 Q846Q0	1AJK[A,B] 1AXK[B] 1BYH[A] 1CPM[A] 1CPN[A] 1MAC[A,B] 1U0A[A,B,C,D] 2AYH[A]
κ-carrageenase (CgkA)	3.2.1.83	Pseudoalteromonas carrageenovora ATCC 43555	AAW20552.1 CAA50624.1	P43478	1DYP[A]
laminarinase (LamR)	3.2.1.39	Rhodothermus marinus ITI278	AAC69707.1	O52754	3ILN[A,B]
endo-β-1,3-glucanase (Curd1;Curd2;Curd3)	3.2.1.39	Streptomyces sioyaensis	AAF31438.1	Q9L816	3DGT[A]
laminarinase (Lam;TmLam;TM0024) (Lam16)	3.2.1.39	Thermotoga maritima MSB8	AAD35118.1 NP_227840.1	Q9WXN1	3AZX[A,B] 3AZY[A,B,C,D] 3AZZ[A,B,C,D] 3B00[A,B,C,D] 3B01[A,B,C,D]
β-1,3-1,4-glucanase / lichenase (Bgl14A1)		uncultured murine large bowel bacterium BAC 14	AAX16366.1	Q58WW0	3I4I[A,B]
β-porphyranase B (PorB;AgaH;zobellia_1017)	3.2.1.178	Zobellia galactanivorans DsiJT	CAZ95074.1 CBM41181.1		3JUU[A,B]
β-porphyranase A (PorA;AgaE;zobellia_2600)	3.2.1.178	Zobellia galactanivorans DsiJT	CAZ96750.1 CBM41182.1		3ILF[A]
β-agarase D (AgaD;zobellia_4243)	3.2.1.81	Zobellia galactanivorans DsiJT	CAZ98378.1 CBM41186.1	D7GXG4	cryst
β-agarase B (AgaB; zobellia_3573)	3.2.1.81	Zobellia galactanivorans DsiJT	AAF21821.1 AAO99183.1 CAC07427.1 CAZ97711.1	Q9RGX8	1O4Z[A,B,C,D]
β-agarase A (AgaA;zobellia_4203)	3.2.1.81	Zobellia galactanivorans DsiJT	AAF21820.1 AAO99182.1 AAO99184.1 CAC07425.1 CAZ98338.1	Q9RGX9	1O4Y[A] 1URX[A]
Eukaryota
Protein Name	EC#	Organism	GenBank	Uniprot	PDB/3D
β-1,3-1,6- / β-1,3-1,4-glucan hydrolase (laminarinase) (Lam16A)	3.2.1.6	Phanerochaete chrysosporium K-3	BAC67687.1	Q874E3	2CL2[A] 2W39[A] 2W52[A] 2WLQ[A] 2WNE[A]
xyloglucan endotransglycosylase 16A (PttXET16-34;XTH16-34) (Xet16A)	2.4.1.207	Populus tremula x Populus tremuloides	AAN87142.1 CBD24810.1	Q8GZD5	1UMZ[A,B] 1UN1[A,B]
xyloglucan endotransferase 1 / xyloglucanase (NXG1;NXET)	2.4.1.207 3.2.1.151	Tropaeolum majus	CAA48324.1	Q07524	2UWA[A,B,C] 2UWB[A,B] 2UWC[A,B] 2VH9[A,B]