Glycoside Hydrolase Family 16

Activities in Familyxyloglucan:xyloglucosyltransferase (EC 2.4.1.207); keratan-sulfate endo-1,4-β-galactosidase (EC 3.2.1.103); endo-1,3-β-glucanase (EC 3.2.1.39); endo-1,3(4)-β-glucanase (EC 3.2.1.6); licheninase (EC 3.2.1.73); β-agarase (EC 3.2.1.81); κ-carrageenase (EC 3.2.1.83); xyloglucanase (EC 3.2.1.151); endo-β-1,3-galactanase (EC 3.2.1.181); β-porphyranase (EC 3.2.1.178); hyaluronidase (EC 3.2.1.35); endo-β-1,4-galactosidase (EC 3.2.1.-); chitin β-1,6-glucanosyltransferase (EC 2.4.1.-); endo-β-1,4-galactosidase (EC 3.2.1.-)
Mechanism Retaining
ClanGH-B
3D Structure Statusβ-jelly roll
Catalytic Nucleophile/BaseGlu (experimental)
Catalytic Proton DonorGlu (experimental)
External resourcesCAZypedia; HOMSTRAD; PRINTS; PROSITE; XTH-World;
Commercial Enzyme Provider(s)MEGAZYME; NZYTech; PROZOMIX;
Statistics GenBank accession (7290); Uniprot accession (1530); PDB accession (83); 3D entries (40); cryst (1)
All (6058) Archaea (21) Bacteria (3460) Eukaryota (2517) Viruses (50) unclassified (10) Structure (40 - 1 cryst) Characterized (210)
Archaea
Protein Name EC#OrganismGenBankUniprot
PDB/3D Carbohydrate Ligands Resolution (Å)
 endo-β-1,3-glucanase (LamA;PF0076) (Lam16) 3.2.1.39 Pyrococcus furiosus DSM 3638 AAC25554.2
AAL80200.1
NP_577805.1
E7FHE7
O73951
2VY0[A,B] 2.16
Bacteria
Protein Name EC#OrganismGenBankUniprot
PDB/3D Carbohydrate Ligands Resolution (Å)
 lichenase / β-1,3-1,4-glucanase (Bg1;LicA) 3.2.1.73 Bacillus licheniformis CAA40547.1 P27051
1GBG[A] 1.80
3D6E[A,B] 2.40
 lichenase (BglS;LicS;Bgl;BSU39070) 3.2.1.73 Bacillus subtilis subsp. subtilis str. 168 CAA86922.1
AAM08358.1
AIY95225.1
AQR83949.1
AQR88164.1
CAA25328.1
CAB15943.1
CAJ15097.1
NP_391786.1
P04957
3O5S[A] 2.20
 β-1,3-1,4-glucanase (BACOVA_02741; Bovatus_03149) 3.2.1.6 Bacteroides ovatus ATCC 8483 ALJ47757.1
EDO11532.1
 
5NBO[A,B]
5NBP[A,B]
 β-porphyranase (BpGH16B;BACPLE_01689) 3.2.1.178 Bacteroides plebeius DSM 17135 EDY95423.1 B5CY92
4AWD[A,B] 2.40
 BuGH16B   Bacteroides uniformis NP1    
5T9X[B,C] 2.50
5T9X[A] 2.50
 β-1,3-glucanase II (BglII) 3.2.1.39 Cellulosimicrobium cellulans LL-G109 / DSM10297 / DK-1 AAC38290.1
AAB39377.1
AAB39378.1
AAB47151.1
AAB47152.1
AAB47153.1
AEK80404.1
EU589324
O68641
3ATG[A] 1.66
 GlcNAc-α-1,4-Gal-releasing endo-β-galactosidase (GngC) 3.2.1.- Clostridium perfringens ATCC 10543 BAB69460.1 Q934G8
1UPS[A,B] 1.82
 lichenase / endo-β-1,3-1,4-glucanase (TF-glucanase;Fisuc_2961;FSU_0226) 3.2.1.73 Fibrobacter succinogenes subsp. succinogenes S85 AAA24896.1
ACR92625.1
ACR92630.1
ACR92631.1
ACR92632.1
ACR92633.1
ACR92636.1
ACR92637.1
ACX76541.1
ADL24776.1
P17989
1MVE[A] 1.70
1ZM1[A,B] β-D-Glcp-(1-4)-β-D-Glcp-(1-3)-β-D-Glcp
2.30
2R49[A] 2.20
2R4A[A] 1.70
3AXD[A,B] 1.53
3AXE[A] β-D-Glcp-(1-4)-β-D-Glcp
1.53
3H0O[A] 1.40
3HR9[A] 1.70
 β-agarase (AgaA;MtAgaA) 3.2.1.81 Microbulbifer thermotolerans JAMB-A94 BAD29947.1
BAK08910.1
Q6F4N4
3WZ1[A] 1.60
 MAB_1840c   Mycobacterium abscessus ATCC 19977 CAM61923.1  
5DXD[A,B] 1.70
 MAP_1661c / MAP1661c   Mycobacterium avium subsp. paratuberculosis K-10 AAS03978.1
NP_960595.1
 
4XXP[A] 1.60
 MFORT_18233   Mycobacterium fortuitum subsp. fortuitum DSM 46621 = ATCC 6841 EJZ11896.1  
4W65[A] 1.38
 β-1,3-glucanase (MMAR_2902)   Mycobacterium marinum M ACC41342.1 B2HE62
4PQ9[A,B] 1.20
 MSMEG_5345   Mycobacterium smegmatis str. MC2 155 ABK72351.1
AFP41643.1
AIU10372.1
A0R351
3RQ0[A] 2.02
 Rv0315   Mycobacterium tuberculosis H37Rv CAB09586.1
AFN48170.1
CCP43045.1
NP_214829.1
O07242
4WZF[A,B] 1.70
 β-1,3-glucanase F (BglF) 3.2.1.39 Nocardiopsis sp. F96 BAE54302.1 Q2V0H0
2HYK[A] 1.30
 lichenase M (Bgi) 3.2.1.73 Paenibacillus macerans CAA39426.1
AAO66468.1
P23904
Q846Q0
1AJK[A,B] 1.80
1AXK[B] 2.10
1BYH[A] β-D-Glcp-(1-4)-β-D-Glcp-(1-1)-<non_carb>
2.80
1CPM[A] 2.00
1CPN[A] 1.80
1MAC[A,B] 2.30
1U0A[A,B,C,D] β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp-(1-3)-β-D-Glcp
1.64
2AYH[A] 1.60
 κ-carrageenase (CgkA) 3.2.1.83 Pseudoalteromonas carrageenovora ATCC 43555 CAA50624.1
AAW20552.1
P43478
1DYP[A] 1.54
5OCQ[A,B]
 laminarinase (LamR) 3.2.1.39 Rhodothermus marinus ITI278 AAC69707.1 O52754
3ILN[A,B] 1.95
 lichenase B (LicB;CtLic16A;Cthe_0211) (Lic16A) 3.2.1.73 Ruminiclostridium thermocellum ATCC 27405 ABN51452.1
CAA44959.1
A3DBX3
3WVJ[A,B] 1.95
 lichenase A / laminarinase (LicA) (Lic16A) 3.2.1.6
3.2.1.73
Ruminiclostridium thermocellum DSM 1237 CAA61884.2 Q59328
cryst    
 endo-β-1,3-glucanase (Curd1;Curd2;Curd3) 3.2.1.39 Streptomyces sioyaensis AAF31438.1 Q9L816
3DGT[A] 1.50
 laminarinase (Lam;TmLam;TM0024) (Lam16) 3.2.1.39 Thermotoga maritima MSB8 AAD35118.1
AGL48947.1
AHD18205.1
AKE27833.1
NP_227840.1
Q9WXN1
3AZX[A,B] 1.80
3AZY[A,B,C,D] 1.65
3AZZ[A,B,C,D] α-D-Glcp
β-D-Glcp
1.81
3B00[A,B,C,D] 1.74
3B01[A,B,C,D] 1.87
 endo-β-1,3-glucanase / laminarinase (Lam;TpLam;Tpet_0899) 3.2.1.39 Thermotoga petrophila RKU-1 ABQ46917.1 A5IL44
4DFS[A,B] 3.75
 β-1,3-1,4-glucanase / lichenase (Bgl14A1)   uncultured murine large bowel bacterium BAC 14 AAX16366.1 Q58WW0
3I4I[A,B] 1.89
 κ-carrageenase (CgkA;zobellia_236) 3.2.1.83 Zobellia galactanivorans DsiJT CAZ94309.1
AAC27890.1
AAW20553.1
CAH61386.1
O84907
5OCR[A,B,C,D]
 β-porphyranase B (PorB;AgaH;zobellia_1017) 3.2.1.178 Zobellia galactanivorans DsiJT CAZ95074.1
CBM41181.1
D7GXF9
3JUU[A,B] 1.80
 β-porphyranase A (PorA;AgaE;zobellia_2600) 3.2.1.178 Zobellia galactanivorans DsiJT CAZ96750.1
CBM41182.1
D7GXG0
3ILF[A] β-L-Galp6SO3-(1-3)-β-D-Galp-(1-4)-α-L-Galp6SO3-(1-3)-α-D-Galp
1.80
4ATE[A] 1.10
 β-agarase D (AgaD;zobellia_4243) 3.2.1.81 Zobellia galactanivorans DsiJT CAZ98378.1
CBM41186.1
D7GXG4
4ASM[B] 1.50
 laminarinase (LamC;ZgLamC;ZgLamCGH16;zobellia_1010) 3.2.1.39 Zobellia galactanivorans DsiJT CAZ95067.1  
4CRQ[A,B] 1.50
4CTE[A,B] β-D-Glcp-(1-3)-β-D-Glcp-(1-3)-β-D-Glcp1SH
β-D-Glcp-(1-3)-β-D-Glcp1SH
1.80
 algal laminarin-specific β-glucanase / laminarinase (LamA;ZgLamA;zobellia_2431) 3.2.1.39 Zobellia galactanivorans DsiJT CAZ96583.1  
4BOW[A,B] β-D-Glcp-(1-3)-β-D-Glcp-(1-3)-α-D-Glcp
β-D-Glcp-(1-3)-β-D-Glcp-(1-3)-β-D-Glcp-(1-3)-β-D-Glcp
1.35
4BPZ[A,B] β-D-Glcp-(1-4)-β-D-Glcp-(1-3)-α-D-Glcp
1.13
4BQ1[A,B] 1.50
 β-agarase B (AgaB; zobellia_3573) 3.2.1.81 Zobellia galactanivorans DsiJT CAZ97711.1
AAF21821.1
AAO99183.1
CAC07427.1
Q9RGX8
1O4Z[A,B,C,D] 2.30
4ATF[A,B,C,D] α-L-3,6-anhydro-Galp-(1-3)-β-D-Galp-(1-4)-α-L-3,6-anhydro-Galp-(1-3)-β-D-Galp-(1-4)-α-L-3,6-anhydro-Galp-(1-3)-β-D-Galp-(1-4)-α-L-3,6-anhydro-Galp-(1-3)-β-D-Galp
1.90
 β-agarase A (AgaA;zobellia_4203) 3.2.1.81 Zobellia galactanivorans DsiJT CAZ98338.1
AAF21820.1
AAO99182.1
AAO99184.1
CAC07425.1
G0L322
Q9RGX9
1O4Y[A] 1.48
1URX[A] α-L-3,6-anhydro-Galp-(1-3)-β-D-Galp-(1-4)-α-L-3,6-anhydro-Galp-(1-3)-β-D-Galp-(1-4)-α-L-3,6-anhydro-Galp-(1-3)-β-D-Galp
α-L-3,6-anhydro-Galp-(1-3)-β-D-Galp-(1-4)-α-L-3,6-anhydro-Galp-(1-3)-β-D-Galp
α-L-3,6-anhydro-Galp-(1-3)-β-D-Galp-(1-4)-α-L-3,6-anhydro-Galp-(1-3)-β-D-Galp-(1-4)-α-L-3,6-anhydro-Galp-(1-3)-β-D-Galp-(1-4)-α-L-3,6-anhydro-Galp
1.7
Eukaryota
Protein Name EC#OrganismGenBankUniprot
PDB/3D Carbohydrate Ligands Resolution (Å)
 exo-β-1,3-glucosyltransferase / elongating β-transglucosylase (Bgt16A;PtBgt16A) 2.4.1.- Paecilomyces sp. J18 / Paecilomyces thermophila J18 ARV79861.1  
5JVV[A,B] 1.59
 β-1,3-1,4-glucanase (PtLic16A) (Lic16A) 3.2.1.73 Paecilomyces sp. J18 / Paecilomyces thermophila J18 ADK55597.1 E0XN39
3WDT[A,B,C,D] 1.98
3WDU[A,B,C,D] β-D-Glcp
β-D-Glcp-(1-4)-β-D-Glcp
2.25
3WDV[A,B,C,D] β-D-Glcp
β-D-Glcp-(1-4)-β-D-Glcp
1.94
3WDW[A,B] 1.80
3WDX[A,B] β-D-Glcp-(1-4)-β-D-Glcp-(1-3)-α-D-Glcp
β-D-Glcp-(1-4)-β-D-Glcp-(1-3)-β-D-Glcp
1.90
3WDY[A,B,C,D] β-D-Glcp
β-D-Glcp-(1-4)-β-D-Glcp
β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp
1.94
 β-1,3-1,6- / β-1,3-1,4-glucan hydrolase (laminarinase) (Lam16A) 3.2.1.6 Phanerochaete chrysosporium K-3 BAC67687.1 Q874E3
2CL2[A] 1.35
2W39[A] β-D-Glcp-(1-3)-β-D-Glcp
1.10
2W52[A] β-D-Glcp-(1-3)-β-D-Glcp-(1-3)-β-D-Glcp
β-D-Glcp-(1-6)-β-D-Glcp-(1-3)-β-D-Glcp-(1-3)-β-D-Glcp
1.56
2WLQ[A] β-D-Glcp-(1-3)-β-D-Glcp-(1-3)-β-D-Glcp-(1-3)-β-D-Glcp-(1-3)-β-D-Glcp-(1-3)-β-D-Glcp-(1-3)-β-D-Glcp
1.40
2WNE[A] β-D-Glcp-(1-3)-β-D-Glcp-(1-3)-β-D-Glcp-(1-3)-β-D-Glcp-(1-3)-β-D-Glcp-(1-3)-β-D-Glcp-(1-3)-α-D-Glcp
2.12
 xyloglucan endotransglycosylase 16A (PttXET16-34;XTH16-34) (Xet16A) 2.4.1.207 Populus tremula x Populus tremuloides AAN87142.1
CBD24810.1
Q8GZD5
1UMZ[A,B] β-D-Galp-(1-2)-α-D-Xylp-(1-6)[α-D-Xylp-(1-6)-β-D-Glcp-(1-4)]-β-D-Glcp-(1-4)-D-1-deoxy-Glcp
1.8
1UN1[A,B] 2.1
 xyloglucanase (NXG1;NXET) (formerly xyloglucan endotransferase 1) 3.2.1.151 Tropaeolum majus CAA48324.1 Q07524
2UWA[A,B,C] 1.8
2UWB[A,B] 2.0
2UWC[A,B] 2.30
2VH9[A,B] β-D-Galp-(1-2)-α-D-Xylp-(1-6)[α-D-Xylp-(1-6)-β-D-Glcp-(1-4)]-β-D-Glcp-(1-4)[β-D-Galp-(1-2)-α-D-Xylp-(1-6)]-β-D-Glcp-(1-4)-α-D-Glcp-(1-4)-GLU
2.1
 broad-specificity endo-(xylo)glucanase (VVEG16) 3.2.1.151
3.2.1.73
Vitis vinifera XP_002273975.1  
5DZE[A] β-D-Glcp
β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-α-D-Glcp
0.97
5DZF[A,B] β-D-Glcp
β-D-Glcp-(1-3)-β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-α-D-Glcp
β-D-Glcp-(1-4)-β-D-Glcp-(1-3)-β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp-(1-3)-β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-α-D-Glcp
1.65
5DZG[A,B] α-D-Xylp-(1-6)-β-D-Glcp-(1-4)[α-D-Xylp-(1-6)]-β-D-Glcp-(1-4)[α-D-Xylp-(1-6)]-β-D-Glcp-(1-4)-α-D-Glcp
α-D-Xylp-(1-6)-β-D-Glcp-(1-4)[α-D-Xylp-(1-6)]-β-D-Glcp-(1-4)[α-D-Xylp-(1-6)]-β-D-Glcp-(1-4)-β-D-Glcp-(1-4)[α-D-Xylp-(1-6)]-β-D-Glcp-(1-4)[α-D-Xylp-(1-6)]-β-D-Glcp-(1-4)[α-D-Xylp-(1-6)]-β-D-Glcp-(1-4)-α-D-Glcp
1.79
5SV8[A] α-D-Xylp-(1-6)-β-D-Glcp-(1-4)-β-D-Glcp-(1-4)[α-D-Xylp-(1-6)]-β-D-Glcp-(1-4)-β-D-Glcp
1.59
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