Glycoside Hydrolase Family 13 / Subf 6

Activities in Sub Family	[retaining] α-amylase (EC 3.2.1.1); [retaining] maltohexaose-producing α-amylase (EC 3.2.1.98);
Mechanism	Retaining a
Clan	GH-H
3D Structure Status	( β / α ) ₈ barrel
Catalytic Nucleophile/Base	Asp (experimental)
Catalytic Proton Donor	Glu (experimental)
Note	New: many members have been assigned to subfamilies as described by Stam et al. (2006) Protein Eng Des Sel. 19, 555-562 (PMID: 17085431)
External resources	CAZypedia; EBI Protein of the Month; HOMSTRAD; PDB Molecule of the Month; PRINTS;
Commercial Enzyme Provider(s)	MEGAZYME; PROZOMIX;
Statistics	GenBank accession (77); Uniprot accession (33); PDB accession (13); 3D entries (3); cryst (0)

Eukaryota

Protein Name

EC#

Organism

GenBank

Uniprot

PDB/3D

Carbohydrate Ligands

Resolution (Å)

Subf

α-amylase 1 (Amy1;Amy1.1)

AAA32929.1
AAA32927.1

1HT6[A]		1.50
1P6W[A]	β-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-β-D-Glcp-(1-1)-methyl β-D-Glcp-α-D-Glcp4S-(1-4)-α-D-Glcp4S-(1-4)-β-D-Glcp4S-(1-1)-methyl	2.00
1RP8[A]	α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-β-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-β-D-Glcp	2.00
1RP9[A]	<non_carb>-(1-4)-α-D-6-deoxy-Glcp4N-(1-4)-α-D-Glcp-(1-4)-β-D-Glcp <non_carb>-(1-4)-α-D-6-deoxy-Glcp4N-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp	2.00
1RPK[A]	<non_carb>-(1-4)-α-D-6-deoxy-Glcp4N-(1-4)-α-D-Glcp-(1-4)-β-D-Glcp <non_carb>-(1-4)-α-D-6-deoxy-Glcp4N-(1-4)-β-D-Glcp	2.00
2QPS[A]		2.20
2QPU[A,B,C]	<non_carb>-(1-4)-α-D-6-deoxy-Glcp4N-(1-4)-D-1-deoxy-Glcp <non_carb>-(1-4)-α-D-6-deoxy-Glcp4N-(1-4)-α-D-Glcp-(1-4)-β-D-Glcp β-D-Glcp	1.70
3BSG[A]		1.95
3BSH[A]	β-D-Glcp	3.00

α-amylase 2 (Amy2)

CAA33298.1
AAA32925.1
AAA98790.1

1AMY[A]		2.80
1AVA[A,B]		1.90
1BG9[A]	<non_carb>-(1-4)-α-D-6-deoxy-Glcp4N-(1-4)-β-D-Glcp <non_carb>-(1-4)-β-D-6-deoxy-Glcp4N	2.80

α-amylase 1A / I-1 (α-Amy7;RAmy1A;Amy1.1;AmyI-1;Os02g0765600)

BAF10134.1
ACA50496.1
BAD17125.1
BAD17313.1
BAG95208.1
BAS81060.1
CAA34516.1

2.16