Glycoside Hydrolase Family 13 / Subf 6

Activities in Familyα-amylase (EC 3.2.1.1); pullulanase (EC 3.2.1.41); cyclomaltodextrin glucanotransferase (EC 2.4.1.19); cyclomaltodextrinase (EC 3.2.1.54); trehalose-6-phosphate hydrolase (EC 3.2.1.93); oligo-α-glucosidase (EC 3.2.1.10); maltogenic amylase (EC 3.2.1.133); neopullulanase (EC 3.2.1.135); α-glucosidase (EC 3.2.1.20); maltotetraose-forming α-amylase (EC 3.2.1.60); isoamylase (EC 3.2.1.68); glucodextranase (EC 3.2.1.70); maltohexaose-forming α-amylase (EC 3.2.1.98); maltotriose-forming α-amylase (EC 3.2.1.116); branching enzyme (EC 2.4.1.18); trehalose synthase (EC 5.4.99.16); 4-α-glucanotransferase (EC 2.4.1.25); maltopentaose-forming α-amylase (EC 3.2.1.-) ; amylosucrase (EC 2.4.1.4) ; sucrose phosphorylase (EC 2.4.1.7); malto-oligosyltrehalose trehalohydrolase (EC 3.2.1.141); isomaltulose synthase (EC 5.4.99.11); malto-oligosyltrehalose synthase (EC 5.4.99.15); amylo-α-1,6-glucosidase (EC 3.2.1.33); α-1,4-glucan: phosphate α-maltosyltransferase (EC 2.4.99.16); 6'-P-sucrose phosphorylase (EC 2.4.1.-); amino acid transporter
Activities in Sub Family
Mechanism Retaining
ClanGH-H
3D Structure Status( β / α ) 8
Catalytic Nucleophile/BaseAsp (experimental)
Catalytic Proton DonorGlu (experimental)
NoteNew: many members have been assigned to subfamilies as described by Stam et al. (2006) Protein Eng Des Sel. 19, 555-562 (PMID: 17085431)
External resourcesCAZypedia; EBI Protein of the Month; HOMSTRAD; PDB Molecule of the Month; PRINTS;
Commercial Enzyme Provider(s)MEGAZYME; PROZOMIX;
Statistics GenBank accession (1207); Uniprot accession (175); PDB accession (13); 3D entries (3); cryst (0)
Eukaryota
Protein Name EC# OrganismGenBankUniprot
PDB/3D Carbohydrate Ligands Resolution (Å)
Subf
 α-amylase 1 (Amy1;Amy1.1) 3.2.1.1 Hordeum vulgare AAA32929.1
AAA32927.1
P00693
Q40017
1HT6[A] 1.50
1P6W[A] β-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-β-D-Glcp-(1-1)-methyl
β-D-Glcp-α-D-Glcp4S-(1-4)-α-D-Glcp4S-(1-4)-β-D-Glcp4S-(1-1)-methyl
2.00
1RP8[A] α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp
α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-β-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-β-D-Glcp
2.00
1RP9[A] <non_carb>-(1-4)-α-D-6-deoxy-Glcp4N-(1-4)-α-D-Glcp-(1-4)-β-D-Glcp
<non_carb>-(1-4)-α-D-6-deoxy-Glcp4N-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp
2.00
1RPK[A] <non_carb>-(1-4)-α-D-6-deoxy-Glcp4N-(1-4)-α-D-Glcp-(1-4)-β-D-Glcp
<non_carb>-(1-4)-α-D-6-deoxy-Glcp4N-(1-4)-β-D-Glcp
2.00
2QPS[A] 2.20
2QPU[A,B,C] <non_carb>-(1-4)-α-D-6-deoxy-Glcp4N-(1-4)-D-1-deoxy-Glcp
<non_carb>-(1-4)-α-D-6-deoxy-Glcp4N-(1-4)-α-D-Glcp-(1-4)-β-D-Glcp
β-D-Glcp
1.70
3BSG[A] 1.95
3BSH[A] β-D-Glcp
3.00
6
 α-amylase 2 (Amy2) 3.2.1.1 Hordeum vulgare CAA33298.1
AAA32925.1
AAA98790.1
P04063
Q40015
1AMY[A] 2.80
1AVA[A,B] 1.90
1BG9[A] <non_carb>-(1-4)-α-D-6-deoxy-Glcp4N-(1-4)-β-D-Glcp
<non_carb>-(1-4)-β-D-6-deoxy-Glcp4N
2.80
6
 α-amylase 1A / I-1 (α-Amy7;RAmy1A;Amy1.1;AmyI-1;Os02g0765600) 3.2.1.1 Oryza sativa Japonica Group BAF10134.1
ACA50496.1
BAD17125.1
BAD17313.1
BAG95208.1
BAS81060.1
CAA34516.1
P17654
Q0DXA4
3WN6[A,B,C,D] 2.16
6
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