Glycoside Hydrolase Family 12

Activities in Familyendoglucanase (EC 3.2.1.4); xyloglucan hydrolase (EC 3.2.1.151); β-1,3-1,4-glucanase (EC 3.2.1.73); xyloglucan endotransglycosylase (EC 2.4.1.207)
Mechanism Retaining
ClanGH-C
3D Structure Statusβ-jelly roll
Catalytic Nucleophile/BaseGlu (experimental)
Catalytic Proton DonorGlu (experimental)
Noteformerly known as cellulase family H.
External resourcesCAZypedia; HOMSTRAD;
Commercial Enzyme Provider(s)MEGAZYME;
Statistics GenBank accession (929); Uniprot accession (212); PDB accession (46); 3D entries (16); cryst (1)
All (737) Archaea (81) Bacteria (514) Eukaryota (141) unclassified (1) Structure (16 - 1 cryst) Characterized (68)
Archaea
Protein Name EC#OrganismGenBankUniprot
PDB/3D Carbohydrate Ligands Resolution (Å)
 endo-β-1,4-glucanase A (EglA;EGPf;TCel1;PF0854) (Cel12) 3.2.1.4 Pyrococcus furiosus DSM 3638 AAD54602.1
AAE50601.1
AAL80978.1
AAM56799.1
AAW12536.1
ABJ38334.1
ACM94275.1
ACW20099.1
AEG76943.1
NP_578583.1
E7FHY8
Q9V2T0
3VGI[A] 1.07
3WQ0[A] β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp
1.22
3WQ1[A] β-D-Glcp-(1-4)-β-D-Glcp-(1-3)-β-D-Glcp-(1-4)-β-D-Glcp
1.30
3WQ7[A,B] 1.68
3WR0[A] 1.16
3WT3[A,B] 1.68
3WXP[A] β-D-Glcp-(1-4)-β-D-Glcp
1.42
3WY6[A] β-D-Glcp
β-D-Glcp-(1-3)-β-D-Glcp
1.45
Bacteria
Protein Name EC#OrganismGenBankUniprot
PDB/3D Carbohydrate Ligands Resolution (Å)
 endo-β-1,4-glucanase / xyloglucanase (XG12;BLi03304;BL02586) (Cel12A) 3.2.1.151
3.2.1.4
Bacillus licheniformis DSM 13 = ATCC 14580 AAU42138.1
AAU24774.1
Q65FM6
2JEM[A,B] 1.78
2JEN[A] α-D-Xylp-(1-6)-β-D-Glcp-(1-4)[α-D-Xylp-(1-6)]-β-D-Glcp
α-D-Xylp-(1-6)[β-D-Glcp-(1-4)]-β-D-Glcp-(1-4)[α-D-Xylp-(1-6)]-β-D-Glcp-(1-4)-β-D-Glcp
1.4
 cellulase A (CelA) (Cel12A) 3.2.1.4 Rhodothermus marinus ITI378 AAB65594.1
AAR63650.1
AAR67703.1
ABJ29234.1
ACP76253.1
O33897
1H0B[A,B] 1.8
2BW8[A,B] 1.54
2BWA[A,B] β-D-Glcp-(1-4)-β-D-Glcp
β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp
1.68
2BWC[A,B] β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp-(1-1)-<non_carb>
2.15
3B7M[A,B,C,D] 2.10
 endo-β-1,4-glucanase (CelB;CelB2;EGI) 3.2.1.4 Streptomyces lividans 1326 AAB71950.1
AAR63649.1
AAR67702.1
ABH87634.1
ABJ29233.1
ACP76252.1
Q54331
1NLR[A] 1.75
2NLR[A] β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp2F
1.20
 cellulase 12A (Cel12A) 3.2.1.4 Streptomyces sp. 11AG8 AAF91283.1
AAE59731.1
AAQ25310.1
AAQ31830.1
AAR63648.1
AAR67701.1
ACP76251.1
Q9KIH1
1OA4[A] 1.5
 endo-β-1,4-glucanase A (CelA;TmCel12A;TM1524;TM_1524;Tmari_1532) (Cel12A) 3.2.1.4 Thermotoga maritima MSB8 AGL50456.1
AAD36591.1
AHD18579.1
AKE29285.1
CAA93273.1
NP_229324.1
Q60032
Q9S5X8
3AMH[A,B] 2.09
3AMM[A,B] β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp
1.98
3AMN[A,B] β-D-Glcp-(1-4)-α-D-Glcp
β-D-Glcp-(1-4)-β-D-Glcp
1.47
3AMP[A,B] β-D-Glcp-(1-4)-β-D-Glcp
β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-α-D-Glcp
1.78
3AMQ[A,B,C,D] β-D-Glcp
β-D-Glcp-(1-4)-α-D-Glcp
1.80
3O7O[A,B] 2.41
3VHN[A,B,C,D,E,F,G,H] β-D-Glcp-(1-4)-β-D-Glcp
2.50
3VHO[A,B] 1.93
 cellulase (Lc-CelA) 3.2.1.4 uncultured bacterium AHL27894.1  
3WX5[A,B] 1.85
Eukaryota
Protein Name EC#OrganismGenBankUniprot
PDB/3D Carbohydrate Ligands Resolution (Å)
 endo-β-1,4-glucanase 1 (Cmc1;Cmc-1;FI CM-cellulase;FI-CMCase) 3.2.1.4 Aspergillus aculeatus F-50 BAA00435.1
AAR63632.1
AAR67685.1
ABJ29216.1
ACP76235.1
CAA36757.1
P22669
5GM3[A,B] 1.59
5GM4[A,B,C,D,E,F,G] β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp
1.92
5GM5[A,B,C,D,E,F,G] β-D-Glcp-(1-4)-β-D-Glcp
1.73
 endo-β-1,4-glucanase (xyloglucan-specific) (XEG1) 3.2.1.151 Aspergillus aculeatus KSM 510 AAD02275.1 O94218
3VL8[A] 1.90
3VL9[A,B] α-D-Xylp-(1-6)[β-D-Glcp-(1-4)]-β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp
α-D-Xylp-(1-6)[β-D-Glcp-(1-4)]-β-D-Glcp-(1-4)[α-D-Xylp-(1-6)]-β-D-Glcp-(1-4)-β-D-Glcp
1.20
3VLB[B,D] 2.70
 endo-β-1,4-glucanase (EglA;AnEglA) 3.2.1.4 Aspergillus niger CBS 120.49 / N400 AAE60101.1
AAE84291.1
CAA03652.1
CAA03657.1
CAA11964.1
CAG17891.1
O74705
1KS4[A] 2.50
1KS5[A] 2.10
 xyloglucan-specific endo-β-1,4-glucanase (XegA) 3.2.1.151 Aspergillus niveus PR-2 AEV23011.1  
4NPR[A,B] 2.50
 endoglucanase (EglD;AtEglD;AtGH12;ATEG_09894) 3.2.1.4 Aspergillus terreus NIH2624 EAU30085.1
XP_001218516.1
 
cryst    
 endoglucanase (Cel12A) 3.2.1.4 Humicola grisea ATCC 22081 AAM77714.2
AAR63636.1
AAR63637.1
AAR67689.1
AAR67690.1
ABJ29220.1
ABJ29221.1
ACP76239.1
ACP76240.1
Q8NJY3
1OLR[A] 1.2
1UU4[A] β-D-Glcp-(1-4)-β-D-Glcp
1.49
1UU5[A] β-D-Glcp-(1-4)-β-D-Glcp-(1-3)-β-D-Glcp-(1-4)-β-D-Glcp
1.7
1UU6[A] β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp
1.4
1W2U[A] β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp
1.52
 endoglucanase (Cel12A) 3.2.1.4 Hypocrea schweinitzii ATCC 66965 AAM77711.1
AAE59776.1
AAN19481.1
AAO95540.1
AAQ42102.1
AAR63631.1
AAR67684.1
ABJ29215.1
ACP76234.1
Q8NJY6
1OA3[A,B,C,D] 1.7
 endo-β-1,4-glucanase 3 / III (Egl3;EG3;EGIII;ThEG3;ThEGIII) (Cel12A)   Trichoderma harzianum IOC-3844 AFK32784.1  
4H7M[A,B] 2.07
 endo-β-1,4-glucanase III (Egl3;EGIII;EG III;EG3;TrCel12A) (Cel12A) 3.2.1.151
3.2.1.4
Trichoderma reesei QM9414 AAE59774.1
AAE59775.1
AAN19479.1
AAN19480.1
AAO95538.1
AAO95539.1
AAQ24888.1
AAQ42100.1
AAQ42101.1
AAR60524.1
AAR63629.1
AAR63630.1
AAR67682.1
AAR67683.1
ABE22099.1
ABJ29213.1
ABJ29214.1
ABV71388.1
ACP76232.1
ACP76233.1
BAA20140.1
O00095
1H8V[A,B,C,D,E,F] 1.9
1OA2[A,B,C,D,E,F] 1.5
1OLQ[A,B] 1.7
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