Glycoside Hydrolase Family 12

Known Activities	endoglucanase (EC 3.2.1.4); xyloglucan hydrolase (EC 3.2.1.151); β-1,3-1,4-glucanase (EC 3.2.1.73); xyloglucan endotransglycosylase (EC 2.4.1.207)
Mechanism	Retaining
Clan	GH-C
3D Structure Status	β-jelly roll
Catalytic Nucleophile/Base	Glu (experimental)
Catalytic Proton Donor	Glu (experimental)
Note	formerly known as cellulase family H.
External resources	HOMSTRAD; InterPro; PFAM;
Commercial Enzyme Provider(s)	MEGAZYME;
Statistics	GenBank accession (446); Uniprot accession (198); PDB accession (27); 3D entries (10); cryst (1)

Summary

All (283) Archaea (46) Bacteria (141) Eukaryota (96) Structure (10 - 1 cryst) Characterized (66)

Bacteria
Protein Name	EC#	Organism	GenBank	Uniprot	PDB/3D
endo-β-1,4-glucanase / xyloglucanase (XG12;BLi03304;BL02586) (Cel12A)	3.2.1.151 3.2.1.4	Bacillus licheniformis ATCC 14580 / DSM13	AAU24774.1 AAU42138.1	Q65FM6	2JEM[A,B] 2JEN[A]
cellulase A (CelA) (Cel12A)	3.2.1.4	Rhodothermus marinus ITI378	AAB65594.1 AAR63650.1 AAR67703.1 ABJ29234.1 ACP76253.1	O33897	1H0B[A,B] 2BW8[A,B] 2BWA[A,B] 2BWC[A,B] 3B7M[A,B,C,D]
endo-β-1,4-glucanase (CelB;CelB2;EGI)	3.2.1.4	Streptomyces lividans 1326	AAB71950.1 AAR63649.1 AAR67702.1 ABH87634.1 ABJ29233.1 ACP76252.1	Q54331	1NLR[A] 2NLR[A]
cellulase 12A (Cel12A)	3.2.1.4	Streptomyces sp. 11AG8	AAE59731.1 AAF91283.1 AAQ25310.1 AAQ31830.1 AAR63648.1 AAR67701.1 ACP76251.1	Q9KIH1	1OA4[A]
endo-β-1,4-glucanase A (CelA;TM1524;TM_1524) (Cel12A)	3.2.1.4	Thermotoga maritima MSB8	AAD36591.1 CAA93273.1 NP_229324.1	Q60032 Q9S5X8	3AMH[A,B] 3AMM[A,B] 3AMN[A,B] 3AMP[A,B] 3AMQ[A,B,C,D] 3O7O[A,B]
Eukaryota
Protein Name	EC#	Organism	GenBank	Uniprot	PDB/3D
endo-β-1,4-glucanase 1 (FI CM-cellulase;FI-CMCase)	3.2.1.4	Aspergillus aculeatus F-50	AAR63632.1 AAR67685.1 ABJ29216.1 ACP76235.1 BAA00435.1 CAA36757.1	P22669.1	cryst
endo-β-1,4-glucanase (EglA)	3.2.1.4	Aspergillus niger CBS 120.49 / N400	AAE60101.1 AAE84291.1 CAA03652.1 CAA03657.1 CAA11964.1 CAG17891.1	O74705	1KS4[A] 1KS5[A]
endoglucanase (Cel12A)	3.2.1.4	Humicola grisea ATCC 22081	AAM77714.2 AAR63636.1 AAR63637.1 AAR67689.1 AAR67690.1 ABJ29220.1 ABJ29221.1 ACP76239.1 ACP76240.1	Q8NJY3	1OLR[A] 1UU4[A] 1UU5[A] 1UU6[A] 1W2U[A]
endo-β-1,4-glucanase III (Egl3;EGIII;EG III;EG3) (Cel12A)	3.2.1.4	Hypocrea jecorina QM9414	AAE59774.1 AAE59775.1 AAN19479.1 AAN19480.1 AAO95538.1 AAO95539.1 AAQ24888.1 AAQ42100.1 AAQ42101.1 AAR60524.1 AAR63629.1 AAR63630.1 AAR67682.1 AAR67683.1 ABE22099.1 ABJ29213.1 ABJ29214.1 ABV71388.1 ACP76232.1 ACP76233.1 BAA20140.1	O00095	1H8V[A,B,C,D,E,F] 1OA2[A,B,C,D,E,F] 1OLQ[A,B]
endoglucanase (Cel12A)	3.2.1.4	Hypocrea schweinitzii ATCC 66965	AAE59776.1 AAM77711.1 AAN19481.1 AAO95540.1 AAQ42102.1 AAR63631.1 AAR67684.1 ABJ29215.1 ACP76234.1	Q8NJY6	1OA3[A,B,C,D]