Glycoside Hydrolase Family 12

Known Activities	endoglucanase (EC 3.2.1.4); xyloglucan hydrolase (EC 3.2.1.151); β-1,3-1,4-glucanase (EC 3.2.1.73); xyloglucan endotransglycosylase (EC 2.4.1.207)
Mechanism	Retaining
Clan	GH-C
3D Structure Status	β-jelly roll
Catalytic Nucleophile/Base	Glu (experimental)
Catalytic Proton Donor	Glu (experimental)
Note	formerly known as cellulase family H.
External resources	HOMSTRAD; InterPro; PFAM;
Commercial Enzyme Provider(s)	MEGAZYME;
Statistics	GenBank accession (446); Uniprot accession (198); PDB accession (27); 3D entries (10); cryst (1)

Summary

All (283) Archaea (46) Bacteria (141) Eukaryota (96) Structure (10 - 1 cryst) Characterized (66)

Archaea
Protein Name	EC#	Organism	GenBank	Uniprot	PDB/3D
cellulase (TCel6;Cmaq_1621)	3.2.1.4	Caldivirga maquilingensis IC-167	ABW02444.1 AEG76945.1	A8M9X3
endo-β-1,4-glucanase A (EglA;TCel1;PF0854) (Cel12)	3.2.1.4	Pyrococcus furiosus DSM 3638	AAD54602.1 AAE50601.1 AAL80978.1 AAM56799.1 AAW12536.1 ABJ38334.1 ACM94275.1 ACW20099.1 AEG76943.1 NP_578583.1	Q9V2T0
cellulase (CelS)	3.2.1.4	Sulfolobus solfataricus MT4	CAC39483.1	Q97VS7
cellulase (CelB;SSO2534)	3.2.1.4	Sulfolobus solfataricus P2	AAK42663.1 CAC24331.1 NP_343873.1
endo-β-1,4-glucanase (SSO1949)	3.2.1.4	Sulfolobus solfataricus P2	AAK42142.1 NP_343352.1	Q97X08
endoglucanase / xylanase (SSO1354)	3.2.1.4 3.2.1.8	Sulfolobus solfataricus P2	AAK41590.1 NP_342800.1	Q97YG7
Bacteria
Protein Name	EC#	Organism	GenBank	Uniprot	PDB/3D
endoglucanase (GuxA)	3.2.1.4	Acidothermus cellulolyticus	ABH87623.1 ABH87627.1 ABH87628.1 ABH87635.1
endoglucanase (GuxA;Acel_0615)	3.2.1.4	Acidothermus cellulolyticus 11B ATCC 43068	ABK52388.1	A0LSH8
cellulase (Cel12;Acel_0619)	3.2.1.4	Acidothermus cellulolyticus 11B ATCC 43068	ABK52392.1	A0LSI2
endo-β-1,4-glucanase / xyloglucanase (XG12;BLi03304;BL02586) (Cel12A)	3.2.1.151 3.2.1.4	Bacillus licheniformis ATCC 14580 / DSM13	AAU24774.1 AAU42138.1	Q65FM6	2JEM[A,B] 2JEN[A]
endo-β-1,4-glucanase (Cel12A) (Cel12A)	3.2.1.4	Bacillus licheniformis GXN151	AAP44491.1	Q7X4S4
Rv1090 (Cel12) (fragment)	3.2.1.73	Mycobacterium tuberculosis H37Rv	CAA17206.1 NP_215606.1	O53438
endo-1,4-glucanase S	3.2.1.4	Pectobacterium carotovorum SCC3193	AAA24817.1 AAR63651.1 AAR67704.1 ABJ29235.1 ACP76254.1	P16630.1
endo-1,4-glucanase B (CelB)	3.2.1.4	Pectobacterium carotovorum subsp. carotovorum LY34	AAC02965.2	O31030
cellulase A (CelA) (Cel12A)	3.2.1.4	Rhodothermus marinus ITI378	AAB65594.1 AAR63650.1 AAR67703.1 ABJ29234.1 ACP76253.1	O33897	1H0B[A,B] 2BW8[A,B] 2BWA[A,B] 2BWC[A,B] 3B7M[A,B,C,D]
endo-β-1,4-glucanase (CelA2;Cel2)	3.2.1.4	Streptomyces halstedii JM8	AAC45429.1	O08468
endo-β-1,4-glucanase (CelB;CelB2;EGI)	3.2.1.4	Streptomyces lividans 1326	AAB71950.1 AAR63649.1 AAR67702.1 ABH87634.1 ABJ29233.1 ACP76252.1	Q54331	1NLR[A] 2NLR[A]
cellulase (11AG8)	3.2.1.4	Streptomyces lividans TK23	AAQ31831.1 ABH87633.1
endo-β-1,4-glucanase S (EglS)	3.2.1.4	Streptomyces rochei A2	CAA52139.1	Q59963
cellulase 12A (Cel12A)	3.2.1.4	Streptomyces sp. 11AG8	AAE59731.1 AAF91283.1 AAQ25310.1 AAQ31830.1 AAR63648.1 AAR67701.1 ACP76251.1	Q9KIH1	1OA4[A]
endo-β-1,4-glucanase S1 (CelS1)	3.2.1.4	Streptomyces viridosporus T7A	AAD25090.1	Q9X602
endo-β-1,4-glucanase (Cel)	3.2.1.4	Streptomyces xylophagus KX6	ACN56471.1	D2D3J0
endo-β-1,4-glucanase A (CelA;TM1524;TM_1524) (Cel12A)	3.2.1.4	Thermotoga maritima MSB8	AAD36591.1 CAA93273.1 NP_229324.1	Q60032 Q9S5X8	3AMH[A,B] 3AMM[A,B] 3AMN[A,B] 3AMP[A,B] 3AMQ[A,B,C,D] 3O7O[A,B]
endo-β-1,4-glucanase B (CelB;TM1525) (Cel12B)	3.2.1.4	Thermotoga maritima MSB8	AAD36592.1 CAA93274.1 NP_229325.1	Q60033
endo-β-1,4-glucanase A (CelA)	3.2.1.4	Thermotoga neapolitana	AAC95059.1 CAB06783.1	O08428 P96491
endo-β-1,4-glucanase B (CelB)	3.2.1.4	Thermotoga neapolitana	AAC95060.1 CAB06782.1	P96492 Q798L1
cellulase (TCel2;Tpet_1267)	3.2.1.4	Thermotoga petrophila RKU-1	ABQ47281.1	A5IM58
cellulase (TCel5;Tpet_1268)	3.2.1.4	Thermotoga petrophila RKU-1	ABQ47282.1	A5IM59
Eukaryota
Protein Name	EC#	Organism	GenBank	Uniprot	PDB/3D
endo-β-1,4-glucanase 1 (FI CM-cellulase;FI-CMCase)	3.2.1.4	Aspergillus aculeatus F-50	AAR63632.1 AAR67685.1 ABJ29216.1 ACP76235.1 BAA00435.1 CAA36757.1	P22669.1	cryst
endo-β-1,4-glucanase (xyloglucan-specific) (XEG1)	3.2.1.151	Aspergillus aculeatus KSM 510	AAD02275.1	O94218
endoglucanase (Cel12B)	3.2.1.4	Aspergillus kawachii IFO 4308	AAM77715.1 AAR63634.1 AAR67687.1 ABJ29218.1 ACP76237.1	Q8NJY2
endo-β-1,4-glucanase (CekA)	3.2.1.4	Aspergillus kawachii IFO 4308	AAR63633.1 AAR67686.1 ABJ29217.1 ACP76236.1 BAA02297.1	Q12679
endo-β-1,4-glucanase (xyloglucan-specific) (AN0452.2)	3.2.1.151	Aspergillus nidulans FGSC A4	ABF50842.1 EAA66551.1
endo-β-1,4-glucanase (EglA)	3.2.1.4	Aspergillus niger CBS 120.49 / N400	AAE60101.1 AAE84291.1 CAA03652.1 CAA03657.1 CAA11964.1 CAG17891.1	O74705	1KS4[A] 1KS5[A]
endo-β-1,4-glucanase (EglA;An14g02760)	3.2.1.4	Aspergillus niger CBS 513.88	CAK46524.1	A2R322
xyloglucan-specific endo-β-1,4-glucanase (Xeg12A)	3.2.1.151	Aspergillus niger N402	ABF46829.1	A1XP58
endo-β-1,4-glucanase (CelA)	3.2.1.4	Aspergillus oryzae KBN616	AAR63635.1 AAR67688.1 ABJ29219.1 ACP76238.1 BAA22588.1	O13454
endo-β-1,4-glucanase (CelA;AO090026000102)	3.2.1.4	Aspergillus oryzae RIB40	BAE59597.1
endo-β-1,4-glucanase (EGIII)	3.2.1.4	Bionectria ochroleuca	AAR63642.1 AAR67695.1 ABJ29226.1 ACP76245.1
endoglucanase (Cel12A)	3.2.1.4	Bionectria ochroleuca 1003	AAM77706.1	Q8NJZ1
endoglucanase (Cel12B)	3.2.1.4	Bionectria ochroleuca 1003	AAM77707.1 AAR63643.1 AAR67696.1 ABJ29227.1 ACP76246.1	Q8NJZ0
endoglucanase (Cel12C)	3.2.1.4	Bionectria ochroleuca 1003	AAM77708.1 AAR63644.1 AAR67697.1 ABJ29228.1 ACP76247.1	Q8NJY9
endoglucanase (Cel12D)	3.2.1.4	Bionectria ochroleuca 1003	AAM77709.1 AAR63645.1 AAR67698.1 ABJ29229.1 ACP76248.1	Q8NJY8
endoglucanase (Cel12A)	3.2.1.4	Chaetomium brasiliense CBS 140.50	AAM77701.1 AAR63638.1 AAR67691.1 ABJ29222.1 ACP76241.1	Q8NJZ6
endoglucanase (EG3; EG III)	3.2.1.4	Chrysosporium lucknowense	AAQ38148.1 ACH15006.1
mixed β-1,3-1,4-glucanase (Mlg2)	3.2.1.73	Cochliobolus carbonum	AAF65311.1	Q9P8N6
endoglucanase (Cel12A)	3.2.1.4	Emericella desertorum CBS 653.73	AAM77702.1 AAR63647.1 AAR67700.1 ABJ29231.1 ACP76250.1	Q8NJZ5
endoglucanase II (EG-II;Eg2;EG35) (Cel12)	3.2.1.4	Fomitopsis palustris FFPRI 0507	BAF49602.1	A4PID1
endoglucanase (Cel12A)	3.2.1.4	Fusarium equiseti CBS 185.34	AAM77703.1 AAR63639.1 AAR67692.1 ABJ29223.1 ACP76242.1	Q8NJZ4
endo-β-1,4-glucanase (Cel12g;GtCel12A) (Cel12A)	3.2.1.4	Gloeophyllum trabeum ATCC 11539	AEJ35167.1 AEM23897.1	P84196
endoglucanase (Cel12A)	3.2.1.4	Humicola grisea ATCC 22081	AAM77714.2 AAR63636.1 AAR63637.1 AAR67689.1 AAR67690.1 ABJ29220.1 ABJ29221.1 ACP76239.1 ACP76240.1	Q8NJY3	1OLR[A] 1UU4[A] 1UU5[A] 1UU6[A] 1W2U[A]
endo-β-1,4-glucanase III (Egl3;EGIII;EG III;EG3) (Cel12A)	3.2.1.4	Hypocrea jecorina QM9414	AAE59774.1 AAE59775.1 AAN19479.1 AAN19480.1 AAO95538.1 AAO95539.1 AAQ24888.1 AAQ42100.1 AAQ42101.1 AAR60524.1 AAR63629.1 AAR63630.1 AAR67682.1 AAR67683.1 ABE22099.1 ABJ29213.1 ABJ29214.1 ABV71388.1 ACP76232.1 ACP76233.1 BAA20140.1	O00095	1H8V[A,B,C,D,E,F] 1OA2[A,B,C,D,E,F] 1OLQ[A,B]
endoglucanase (Cel12A)	3.2.1.4	Hypocrea koningii ATCC 2613	AAM77712.1	Q8NJY5
endoglucanase (Cel12A)	3.2.1.4	Hypocrea rufa ATCC 26802	AAM77713.1	Q8NJY4
endoglucanase (Cel12A)	3.2.1.4	Hypocrea schweinitzii ATCC 66965	AAE59776.1 AAM77711.1 AAN19481.1 AAO95540.1 AAQ42102.1 AAR63631.1 AAR67684.1 ABJ29215.1 ACP76234.1	Q8NJY6	1OA3[A,B,C,D]
xyloglucan endotransglycosylase (XET)	2.4.1.207	Macrophomina phaseolina	AAN89225.1 AAS22565.1
endo-β-1,3-1,4-glucanase (MoCel12A;MG00677.4) (Cel12A)	3.2.1.73	Magnaporthe grisea 70-15	XP_368567.1
endo-β-1,3-1,4-glucanase (MoCel12C;MG10972.4) (Cel12C)	3.2.1.73	Magnaporthe grisea 70-15	XP_361895.1
endo-β-1,3-1,4-glucanase (MoCel12B;MG08537.4) (Cel12B)	3.2.1.73	Magnaporthe grisea 70-15	XP_362900.1
xyloglucanase	3.2.1.151	Malbranchea cinnamomea	AAO95986.1
endoglucanase (Cel12A)	3.2.1.4	Nectria ipomoeae CBS 203.32	AAM77704.1 AAR63640.1 AAR67693.1 ABJ29224.1 ACP76243.1	Q8NJZ3
endoglucanase (Cel12B)	3.2.1.4	Nectria ipomoeae CBS 203.32	AAM77705.1 AAR63641.1 AAR67694.1 ABJ29225.1 ACP76244.1	Q8NJZ2
endo-β-1,4-glucanase 3 (Egl3)	3.2.1.4	Penicillium canescens VKPM F178
endoglucanase 28 (Cel12A)	3.2.1.4	Phanerochaete chrysosporium K3	AAU12276.1	Q66NB6
endoglucanase (Cel3A;CMCase3a;CMCase IIIa)	3.2.1.4	Polyporus arcularius 69B-8	BAD98315.1	Q50LE3
endoglucanase (Cel12A)	3.2.1.4	Stachybotrys echinata CBS 304.54	AAM77710.1 AAR63646.1 AAR67699.1 ABJ29230.1 ACP76249.1	Q8NJY7

Footnotes

Experimental characterization exclusively covers enzyme activities at present. If you are aware of any missing experimental characterization that should be mentioned here, we encourage you to send your comment and support of the experimental evidence, including an electronic copy or appropriate link to the support material (i.e., article(s), thesis, database, etc), to cazy@afmb.univ-mrs.fr.