Glycoside Hydrolase Family 5 / Subf 4

Mechanism	Retaining e
Clan	GH-A
3D Structure Status	( β / α ) ₈ barrel
Catalytic Nucleophile/Base	Glu (experimental)
Catalytic Proton Donor	Glu (experimental)
Note	Once known as cellulase family A; many members have been assigned to subfamilies as described by Aspeborg et al. (2012) BMC Evol Biol. 12(1):186 (PMID: 22992189).
External resources	CAZypedia; HOMSTRAD; PROSITE;
Commercial Enzyme Provider(s)	MEGAZYME; NZYTech; PROZOMIX;
Statistics	GenBank accession (490); Uniprot accession (141); PDB accession (58); 3D entries (29); cryst (0)

Activity Id	EC#	Activity Name	Residue -2	Bond -1	Residue -1	Reacting Bond	Residue +1	Bond +1	Residue +2	Reactant
H4	3.2.1.4	cellulose endo-b-1,4-glucosidase	bDGlcp	1,4	bDGlcp	1,4	bDGlcp	1,4	bDGlcp	H2O
H5	3.2.1.6	MLG endo-b-1,3/4-glucosidase	bDGlcp	1,3/4	bDGlcp	1,3/4	bDGlcp	1,3/4	bDGlcp	H2O
H7	3.2.1.8	xylan endo-b-1,4-xylosidase	bDXylp	1,4	bDXylp	1,4	bDXylp	1,4	bDXylp	H2O
H55	3.2.1.73	MLG endo-b-1,4-glucosidase	bDGlcp	1,3/4	bDGlcp	1,4	bDGlcp	1,3/4	bDGlcp	H2O
H59	3.2.1.78	mannan endo-b-1,4-mannosidase	bDManp	1,4	bDManp	1,4	bDManp	1,4	bDManp	H2O
H117	3.2.1.150	reducing end xyloglucan exo-b-1,4-glucobiosidase	(aDXylp-1,6)bDGlcp	1,4	(aDXylp-1,6)bDGlcp	1,4	(aDXylp-1,6)bDGlcp	1,4	DGlcp]	H2O
H118	3.2.1.151	xyloglucan endo-b-1,4-glucosidase	(aDXylp-1,6)bDGlcp	1,4	bDGlcp	1,4	(aDXylp-1,6)bDGlcp	1,4	(aDXylp-1,6)bDGlcp	H2O
H192	3.2.1.-	MLG endo-b-1,3-glucosidase	bDGlcp	1,3/4	bDGlcp	1,3	bDGlcp	1,3/4	bDGlcp	H2O
H228	3.2.1.-	glucomannan endo-b-1,4-glucosidase	bDManp	1,4	bDGlcp	1,4	bDGlcp	1,4	bDManp	H2O
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