Mechanism	Retaining a
Clan	GH-D
3D Structure Status	( β / α ) 8 barrel
Catalytic Nucleophile/Base	Asp (experimental)
Catalytic Proton Donor	Asp (experimental)
Note	Mechanistic commonality with family GH27 demonstrated by Comfort et al. (2007) Biochemistry, 46: 3319-3330 (PMID: 17323919)
External resources	CAZypedia;
Commercial Enzyme Provider(s)	MEGAZYME;
Statistics	GenBank accession (17238); Uniprot accession (110); PDB accession (45); 3D entries (12); cryst (0)

Bacteria
Protein Name	EC#	Organism	GenBank	Uniprot	PDB/3D Carbohydrate Ligands Resolution (Å)
A4V09_19485		Blautia pseudococcoides YL58	ANU77732.1		8K1A[A,B,C,D] 3.28 8K7U[A,B,C,D] 3.37 8K7V[A,B,C,D] 3.17
α-galactosaminidase specific to de-N-acetylated α-galactosaminides (FpGalNase; A4U99_12085)	3.2.1.- 3.2.1.-	Flavonifractor plautii YL31	ANU41754.1 QQR05374.1 UQA26184.1 WP_044942952.1	A0A096B2Z8 A0A1C7FP14 A0AAX1KHI8 P0DTR5	8X1B[A] 2.59 9AWT[A,B,C,D,E] 9AY8[A,B,C,D,E] 2.00 9AYU[A,B,C,D,E] 2.00
α-galactosidase (AgaB;AgaB1)	3.2.1.22	Geobacillus stearothermophilus KVE39	AAG49421.1	Q934H7	4FNQ[A] 1.80
α-galactosidase (AgaA;GsAgaA;Aga1)	3.2.1.22	Geobacillus stearothermophilus KVE39	AAG49420.1	Q9ALJ4	4FNP[A,B,C,D] 2.80 4FNR[A,B,C,D] 3.20 4FNS[A,B,C,D] <carb_like_DGJ> 2.60 4FNT[A,B,C,D] α-D-Galp-(1-6)-α-D-Glcp-(1-2)-β-D-Fruf 2.60 4FNU[A,B,C,D] α-D-Galp-(1-6)-α-D-Galp-(1-6)-α-D-Glcp-(1-2)-β-D-Fruf 3.60
α-galactosidase (MelA; Aga; LBA1438)	3.2.1.22	Lactobacillus acidophilus NCFM	AAO21867.1 AAV43263.1 ACK31409.1	G1UB44 Q7WWP9	2XN0[A,B] 2.50 2XN1[A,B,C,D] 2.30 2XN2[A] α-D-Galp 1.58
LVIS_1758 (LbR11)		Levilactobacillus brevis ATCC 367	ABJ64825.1		3MI6[A,B,C,D] 2.70
α-galactosidase / sucrose kinase (AgaSK; RgAgaSK; RUGNEv3_61222)	3.2.1.22	Mediterraneibacter gnavus E1	CCA61959.1	G4T4R7	2YFN[A] 1.45 2YFO[A] α-D-Galp 1.35
α-galactosidase (SP1898)	3.2.1.22	Streptococcus pneumoniae TIGR4	AAK75969.1 NP_346329.1	A0A0H2URQ6 Q97NW1	6PHU[A] 2.20 6PHV[A] α-D-Galp 2.10 6PHW[A] α-D-Galp-(1-6)-α-D-Glcp 2.20 6PHX[A] α-D-Galp-(1-6)-α-D-Glcp-(1-2)-β-D-Fruf 2.00 6PHY[A] α-D-Galp-(1-3)-α-D-Galp α-D-Galp-(1-3)-β-D-Galp 2.00 6PI0[A] α-D-Galp-(1-3)-β-D-Galp 2.09
α-galactosidase (GalA;TmGalA;TmGal36A;Tm1192) (Gal36)	3.2.1.22	Thermotoga maritima MSB8	CAH68692.1 AAD36267.1 AAM56791.1 AGL50123.1 AHD18901.1 AKE28969.1 CAA04514.1 CAD29026.1 CAT81453.1 NP_228997.1	G4FEF4 O33835	1ZY9[A] 2.34 5M0X[A] 1.80 5M12[A] 1.53 5M16[A] 1.62 5M1I[A] 1.55 6GTA[A] 2.20 6GVD[A] 1.22 6GWF[A] 1.72 6GWG[A] 1.77 6GX8[A] 1.42
α-galactosidase (TtGalA; TTHB115)	3.2.1.22	Thermus thermophilus HB8	BAD71911.1	Q53W51	6LCJ[A,B,C,D,E,F] 2.50 6LCK[A,B,C] α-D-Galp-(1-1)-<non_carb> 2.85 6LCL[A,C,E] α-D-Galp-(1-6)-D-1-deoxy-Galp 3.20
Eukaryota
Protein Name	EC#	Organism	GenBank	Uniprot	PDB/3D Carbohydrate Ligands Resolution (Å)
raffinose exo-α-1,6-galactosidase (AtAkαGal3; At5g20250; DIN10)	3.2.1.- 2.4.1.67	Arabidopsis thaliana	NP_851044.2 AAL90901.1 AAN18198.1 AED92818.1 AED92819.1 AED92820.1 AED92821.1 BAD93984.1 BAE99252.1 BAH19441.1 BAH19983.1 BAH57038.1 NP_197525.1	F4K470 Q8RX87	7EXF[A,B] β-D-Galp 2.17 7EXG[A,B] α-L-Arap β-D-2-deoxy-Galp 2.05 7EXH[A,B] α-L-Talp-(1-1)-<non_carb> 2.63 7EXJ[A,B] 2.47 7EXQ[A,B] α-D-Glcp-(1-2)-β-D-Fruf α-L-Allp α-L-Gulp 2.20 7EXR[A,B] α-D-Galp-(1-6)-α-D-Galp-(1-6)-α-D-Glcp-(1-2)-β-D-Fruf 2.00
α-galactosidase		Paecilomyces sp. thermophila J18	AWW14941.1		6JHP[A,B,C,D] 2.56
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