Mechanism	Retaining a
Clan	GH-H
3D Structure Status	( β / α ) 8 barrel
Catalytic Nucleophile/Base	Asp (experimental)
Catalytic Proton Donor	Glu (experimental)
Note	New: many members have been assigned to subfamilies as described by Stam et al. (2006) Protein Eng Des Sel. 19, 555-562 (PMID: 17085431)
External resources	CAZypedia; EBI Protein of the Month; PDB Molecule of the Month;
Commercial Enzyme Provider(s)	MEGAZYME; PROZOMIX;
Statistics	GenBank accession (11); Uniprot accession (41); PDB accession (25); 3D entries (6); cryst (1)

Bacteria
Protein Name	EC#	Reference	Organism	GenBank	Uniprot	PDB/3D	Subf
pullulanase (PulAg)	3.2.1.41		Anaerobranca gottschalkii	AAS47565.1	Q6QHE9		14
pullulanase	3.2.1.41		Anaerobranca horikoshii	AAP45012.1	Q6XQ00		14
pullulanase type I (PulA)	3.2.1.41		Anoxybacillus sp. LM18-11	AEW23439.1	K9L0H1	3WDH[A] 3WDI[A] 3WDJ[A]	14
pullulanase (PulB)	3.2.1.41	doi	Bacillus acidopullulyticus	CAC60158.1 AAX00709.1 ACK18365.1 CAX87016.1 CAZ67879.1 CAZ67880.1			14
pullulanase (PulA;BaPul13A) (Pul13A)	3.2.1.41		Bacillus acidopullulyticus	CAC60156.1 AAX00707.1 ACK18363.1 CAX87014.1		2WAN[A]	14
type II pullulanase A (PulA)	3.2.1.41		Bacillus cereus GXBC-3	AFP89962.1	J7H570		14
type I pullulanase C (PulC)	3.2.1.41		Bacillus cereus GXBC-3	AFP89964.1	J7H7V3		14
type I pullulanase B (PulB)	3.2.1.41		Bacillus cereus GXBC-3	AFP89963.1	J7H256		14
pullulanase	3.2.1.41	doi	Bacillus cereus OPF-0031	AFJ04510.1	R4I4I6		14
amylopullulanase	3.2.1.41	pubmed	Bacillus deramificans	CAC60157.1 AAX00708.1 ACK18364.1 CAX87015.1			14
isoamylase / pullulanase type I (BsIam; PulA1)	3.2.1.41 3.2.1.68	pubmed pubmed	Bacillus sp. CICIM 263	AGA03915.1	L0C9D3		14
pullulanase type I / glycogen-debranching enzyme (AmyX;BSU29930)	3.2.1.41	pubmed	Bacillus subtilis subsp. subtilis str. 168	AAC00283.1 ACH14984.1 AIY94319.1 AQR83021.1 AQR87236.1 CAB14971.1 CAB14971.2 NP_390871.1	A0A6M4JN50 C0SPA0 O34587	2E8Y[A,B] 2E8Z[A,B] 2E9B[A,B]	14
pullulanase (PulI; BT1663; BT_1663)	3.2.1.41	pubmed	Bacteroides thetaiotaomicron VPI-5482	AAO76770.1 AAC44685.1 NP_810576.1	P71095 Q8A762		14
bifunctional amylopullulanase (ApuB;Bbr_0123)	3.2.1.41		Bifidobacterium breve UCC2003	AAY89038.1 ABE94835.1	F9Y041 Q0R5Z4		14
pullulanase type I (PulA)	3.2.1.41	pubmed	Caldicellulosiruptor saccharolyticus	AAB06264.1	Q59319		14
pullulanase (Pul3YH5)	3.2.1.41		Exiguobacterium acetylicum a1 / YH5	AFL46505.1	I3WU34		14
pullulanase (Pul-SH3)	3.2.1.41		Exiguobacterium sp. Sh3	AJG04842.1	A0A0B5JT51		14
pullulanase I (PulA)	3.2.1.41	pubmed	Fervidobacterium pennivorans Ven5	AAD30387.1	Q9XDB5	cryst	14
amylopullulanase (HMPREF1573_00831)	3.2.1.41	pubmed	Gardnerella vaginalis JCP7276	EPI56559.1			14
pullulanase (GK2827)	3.2.1.41		Geobacillus kaustophilus HTA426	BAD77112.1	Q5KW24		14
pullulanase (PulT)	3.2.1.41	pubmed	Geobacillus stearothermophilus	1808262A			14
pullulanase type I (Orf1)	3.2.1.41		Geobacillus thermoleovorans US105	CAC85704.1	Q8GJ67		14
amylopullulanase (ApuA)	3.2.1.1 3.2.1.41		Lactiplantibacillus plantarum L137	BAF93906.1 BAG67031.1	A9EEQ2 B3Y970		14
pullulanase (LaPul; AmyX; LBA1710)	3.2.1.41	pubmed	Lactobacillus acidophilus NCFM	AAV43522.1	Q5FIF2		14
amylopullulanase (ERD32_11430;PulA)	3.2.1.1 3.2.1.41	pubmed pubmed	Lactobacillus crispatus L49	RXF54749.1	A0A4Q0LMV5		14
amylopullulanase (HMPREF0507_01102)	3.2.1.1 3.2.1.41	pubmed pubmed	Lactobacillus crispatus MV-1A-US	EEU28204.2			14
amylopullulanase (HMPREF9219_0963; PulA)	3.2.1.1 3.2.1.41	pubmed pubmed	Lactobacillus iners LEAF 3008A-a	EFQ51965.1			14
isoamylase (Iam1)	3.2.1.68	doi	Lederbergia lenta JNU3	AGL34022.1	A0A075BIV0		14
pullulanase type I (AmyA1)	3.2.1.41		mixed culture bacterium AmyA1	AGW21593.1	U3MXZ5		14
Pullulanase (type I) (HMPREF0578_0666; PulA)	3.2.1.41	pubmed	Mobiluncus mulieris 28-1	EEZ90738.1	D0YTI0		14
pulullanase type I (PulA;PbPulA)	3.2.1.41		Paenibacillus barengoltzii CAU904	AJP16551.1 WP_016313728.1	A0A0C5GWS2	5WVQ[A] 5WVS[A] 5WVT[A] 5WW1[A] 5WW2[A] 5WWA[A] 5WWB[A] 6JEQ[A] 6JFJ[A] 6JFX[A] 6JHF[A] 6JHG[A] 6JHH[A] 6JHI[A]	14
pullulanase type I (PulN)	3.2.1.41		Paenibacillus polymyxa Nws-pp2	AIE88189.1	A0A075FE92		14
pullulanase (type I) (PulA)	3.2.1.41	pubmed	Prevotella bivia	WP_061450340.1			14
pullulanase	3.2.1.41	URL	Pullulanibacillus naganoensis	AAQ80298.1 AAQ80294.1 AAQ80299.1 AAQ80300.1 AEV53626.1	G9JLV4		14
pullulanase (RBR_10980;Amy10)	3.2.1.41	pubmed	Ruminococcus bromii L2-63	CBL15393.1	D4L6Z8		14
pullulanase (RBR_13560;Amy12)	3.2.1.41	pubmed	Ruminococcus bromii L2-63	CBL15610.1	A0A2N0UU23 D4L7L5	7LSA[A] 7LSR[A] 7LST[A] 7LSU[A]	14
pullulanase (PulA; TmPul13; Tm1845) (Pul13A)	3.2.1.41	pubmed	Thermotoga maritima MSB8	AAD36907.1 AAM56795.1 ABJ38302.1 ABJ38322.1 ACW20067.1 ACW20078.1 AGL50777.1 AHD18265.1 AKE29594.1 CAA04522.1 CAH68696.1 CAT81460.1 NP_229641.1	G4FGN0 O33840		14
pullulanase (PulA)	3.2.1.41	pubmed	Thermotoga neapolitana KCCM 41025	ACN58254.1	C0LLG9		14
pullulanase (Pul)	3.2.1.41		Thermus sp. IM6501	AAC15073.1	O69008		14
pullulanase (Pul)	3.2.1.41		Thermus thermophilus HB8	BAB62095.1	Q7DI19		14
unclassified
Protein Name	EC#	Reference	Organism	GenBank	Uniprot	PDB/3D	Subf
α-amylase	3.2.1.20	pubmed	uncultured organism	ADD61669.1	D9ZEB5		14
amylase	3.2.1.20	pubmed	uncultured organism	ADD61743.1	D9ZEH9		14
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