Glycoside Hydrolase Family 13 / Subf 12

Mechanism	Retaining a
Clan	GH-H
3D Structure Status	( β / α ) ₈ barrel
Catalytic Nucleophile/Base	Asp (experimental)
Catalytic Proton Donor	Glu (experimental)
Note	New: many members have been assigned to subfamilies as described by Stam et al. (2006) Protein Eng Des Sel. 19, 555-562 (PMID: 17085431)
External resources	CAZypedia; EBI Protein of the Month; PDB Molecule of the Month;
Statistics	GenBank accession (1396); Uniprot accession (10); PDB accession (5); 3D entries (2); cryst (0)

Bacteria

Protein Name

EC#

Organism

GenBank

Uniprot

PDB/3D

Carbohydrate Ligands

Resolution (Å)

Subf

pullulanase type I (SAP;GBSCOH1_1122)

CDN66588.1
EAO76676.1

3FAW[A]		2.10
3FAX[A]	α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-β-D-Glcp	2.40

glycogen-degrading enzyme (SpuA;PulA;SP0268)

AAK74446.1
AAQ38066.1
AAT16713.1
AAY71712.1
ABA33035.1
ACK14503.1
ACW45222.1
NP_344806.1

2YA0[A]		1.85
2YA1[A]	α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-β-D-Glcp	2.25
2YA2[A]	α-D-Glcp-(1-1)-<non_carb> <carb_like_NOJ>	2.37