Glycoside Hydrolase Family 5 / Subf 21

Activities in Familyendo-β-1,4-glucanase / cellulase (EC 3.2.1.4); endo-β-1,4-xylanase (EC 3.2.1.8); β-glucosidase (EC 3.2.1.21); β-mannosidase (EC 3.2.1.25); β-glucosylceramidase (EC 3.2.1.45); glucan β-1,3-glucosidase (EC 3.2.1.58); licheninase (EC 3.2.1.73); exo-β-1,4-glucanase / cellodextrinase (EC 3.2.1.74); glucan endo-1,6-β-glucosidase (EC 3.2.1.75); mannan endo-β-1,4-mannosidase (EC 3.2.1.78); cellulose β-1,4-cellobiosidase (EC 3.2.1.91); steryl β-glucosidase (EC 3.2.1.104); endoglycoceramidase (EC 3.2.1.123); chitosanase (EC 3.2.1.132); β-primeverosidase (EC 3.2.1.149); xyloglucan-specific endo-β-1,4-glucanase (EC 3.2.1.151); endo-β-1,6-galactanase (EC 3.2.1.164); hesperidin 6-O-α-L-rhamnosyl-β-glucosidase (EC 3.2.1.168); β-1,3-mannanase (EC 3.2.1.-); arabinoxylan-specific endo-β-1,4-xylanase (EC 3.2.1.-); mannan transglycosylase (EC 2.4.1.-)
Activities in Sub Family
Mechanism Retaining
ClanGH-A
3D Structure Status( β / α ) 8
Catalytic Nucleophile/BaseGlu (experimental)
Catalytic Proton DonorGlu (experimental)
NoteOnce known as cellulase family A; many members have been assigned to subfamilies as described by Aspeborg et al. (2012) BMC Evol Biol. 12(1):186 (PMID: 22992189).
External resourcesCAZypedia; HOMSTRAD; PROSITE;
Commercial Enzyme Provider(s)MEGAZYME; NZYTech; PROZOMIX;
Statistics GenBank accession (21); Uniprot accession (9);
Bacteria
Protein Name EC#Organism GenBankUniprotPDB/3DSubf
 CDL62_06395   Alkalitalea saponilacus DSM 24412 ASB48785.1     21
 CDL62_06245   Alkalitalea saponilacus DSM 24412 ASB48762.1     21
 BACCELL_03415   Bacteroides cellulosilyticus DSM 14838 EEF88964.1     21
 BcellWH2_02825 (Xynb_4)   Bacteroides cellulosilyticus WH2 ALJ60064.1     21
 BcellWH2_04303   Bacteroides cellulosilyticus WH2 ALJ61520.1     21
 endoxylanase (ORF1299;BACEGG_01299) (BeXyn5A) 3.2.1.8 Bacteroides eggerthii DSM 20697 EEC54456.1 B7AFX5   21
 BACINT_01125 (ORF1125)   Bacteroides intestinalis DSM 17393 EDV06040.1 B3C9G1   21
 endo-β-1,4-xylanase (BiXyn5A;ORF4213;BACINT_04213) (Xyn5A) 3.2.1.8 Bacteroides intestinalis DSM 17393 EDV05070.1 B3CET2   21
 BXY_29320   Bacteroides xylanisolvens XB1A CBK67955.1 D6D0K3   21
 Belba_2165   Belliella baltica DSM 15883 AFL84732.1     21
 CHSO_3289   Chryseobacterium sp. StRB126 BAP32326.1     21
 FH5T_02350   Draconibacterium orientale FH5 AHW58816.1     21
 BIW12_10460   Flavobacterium communis PK15 AOZ99822.1     21
 BB050_03462   Flavobacterium johnsoniae GSE09 AOC96551.1     21
 Palpr_1662   Paludibacter propionicigenes WB4 ADQ79803.1 E4T509   21
 xylanase (ORF0336) (Xyn5B) 3.2.1.8 Prevotella bryantii B14 ADI70667.1 D7SFG3   21
 xylanase (ORF0150) (Xyn5A) 3.2.1.8 Prevotella bryantii B14 ADI70668.1 D7SFG4   21
 PRU_2706   Prevotella ruminicola 23 ADE82202.1 D5EXZ3   21
 BV902_20700   Sphingobacterium sp. B29 APU98457.1     21
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