Activities in Sub Family	α-3-isomaltosyltransferase / cycloalternan-forming enzyme (EC 2.4.1.387); [retaining] cycloalternan-specific α-1,3-glucosidase / α-1,3-isomaltosidase (EC 3.2.1.204);
Mechanism	Retaining a
Clan	GH-D
3D Structure Status	( β / α ) 8 barrel
Catalytic Nucleophile/Base	Asp
Catalytic Proton Donor	Asp
Note	Subfamily classification initiated by Arumapperuma et al. 2023 [PMID:36806678]
External resources	CAZypedia; PROSITE;
Commercial Enzyme Provider(s)	MEGAZYME;
Statistics	GenBank accession (426); Uniprot accession (9); PDB accession (13); 3D entries (3); cryst (0)

Bacteria
Protein Name	EC#	Organism	GenBank	Uniprot	PDB/3D Carbohydrate Ligands Resolution (Å)	Subf
cycloalternan-specific α-1,3-glucosidase / α-1,3-isomaltosidase (Kfla_1895)	3.2.1.204	Kribbella flavida DSM 17836	ADB30989.1	D2PPM7	5X3I[A,B] 2.10 5X3J[A,B] 2.30 5X3K[A,B] α-D-Glcp-(1-6)-α-D-Glcp 2.50	7
cycloalternan-forming enzyme / 3-α-isomaltosyltransferase (CAFE;LmCAFE;Lmo2446)	2.4.1.387	Listeria monocytogenes EGD-e	CAD00524.1 NP_465969.1	Q8Y4J2	4KMQ[A] 1.90 4KWU[A] α-D-Glcp β-D-Glcp 1.90 5F7U[A] α-D-Glcp-(1-4)-α-D-Glcp α-D-Glcp-(1-6)-α-D-Glcp α-D-Glcp-(1-6)-α-D-Glcp-(1-3)[α-D-Glcp-(1-6)]-α-D-Glcp α-D-Glcp-(1-6)-α-D-Glcp-(1-4)-α-D-Glcp 1.70 5HPO[A] α-D-Glcp-(1-4)-α-D-Glcp α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp 1.80 5HXM[A] α-D-Glcp-(1-6)-α-D-Glcp 1.90 5I0D[A,B] α-D-Glcp α-D-Glcp-(1-3)-α-D-Glcp-(1-6)-α-D-Glcp α-D-Glcp-(1-6)-α-D-Glcp 1.77	7
cycloalternan-specific α-1,3-glucosidase (CADE;TpCADE;TpLmo0182;CQ11_05330)	3.2.1.204	Trueperella pyogenes TP6375	AHU89502.1	A0A806M5Y7	5F7S[A,B] 2.30 5I0E[B] α-D-Glcp-(1-6)-α-D-Glcp 2.30 5I0F[B] α-D-Glcp-(1-6)-α-D-Glcp α-D-Glcp-(1-6)-β-D-Glcp-(1-4)-ASP 1.85 5I0G[B] 2.15	7
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