Activities in Sub Family	Xyloglucan-specific endo-β-1,4-glucanase / endo-xyloglucanase (EC 3.2.1.151); Lichenase / endo-β-1,3-1,4-glucanase (EC 3.2.1.73);
Mechanism	Retaining e
Clan	GH-B
3D Structure Status	β-jelly roll
Catalytic Nucleophile/Base	Glu (experimental)
Catalytic Proton Donor	Glu (experimental)
Note	Many members have been assigned to subfamilies as described by Viborg et al. (2019) J Biol Chem. 2019 Sep 9 (PMID: 31501245).
External resources	CAZypedia; HOMSTRAD; PRINTS; PROSITE; XTH-World;
Commercial Enzyme Provider(s)	MEGAZYME; NZYTech; PROZOMIX;
Statistics	GenBank accession (2); Uniprot accession (1); PDB accession (4); 3D entries (1); cryst (0)

Eukaryota
Protein Name	EC#	Organism	GenBank	Uniprot	PDB/3D Carbohydrate Ligands Resolution (Å)	Subf
broad-specificity endo-(xylo)glucanase (VVEG16)	3.2.1.73 3.2.1.151	Vitis vinifera	XP_002273975.1	F6I323	5DZE[A] β-D-Glcp β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-α-D-Glcp 0.97 5DZF[A,B] β-D-Glcp β-D-Glcp-(1-3)-β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-α-D-Glcp β-D-Glcp-(1-4)-β-D-Glcp-(1-3)-β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp-(1-3)-β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-α-D-Glcp 1.65 5DZG[A,B] α-D-Xylp-(1-6)-β-D-Glcp-(1-4)[α-D-Xylp-(1-6)]-β-D-Glcp-(1-4)[α-D-Xylp-(1-6)]-β-D-Glcp-(1-4)-α-D-Glcp α-D-Xylp-(1-6)-β-D-Glcp-(1-4)[α-D-Xylp-(1-6)]-β-D-Glcp-(1-4)[α-D-Xylp-(1-6)]-β-D-Glcp-(1-4)-β-D-Glcp-(1-4)[α-D-Xylp-(1-6)]-β-D-Glcp-(1-4)[α-D-Xylp-(1-6)]-β-D-Glcp-(1-4)[α-D-Xylp-(1-6)]-β-D-Glcp-(1-4)-α-D-Glcp 1.79 5SV8[A] α-D-Xylp-(1-6)-β-D-Glcp-(1-4)-β-D-Glcp-(1-4)[α-D-Xylp-(1-6)]-β-D-Glcp-(1-4)-β-D-Glcp 1.59	27
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