Glycoside Hydrolase Family 13 / Subf 32

Activities in Sub Family	[retaining] α-amylase (EC 3.2.1.1); [retaining] maltotriose-producing α-amylase (EC 3.2.1.116);
Mechanism	Retaining a
Clan	GH-H
3D Structure Status	( β / α ) ₈ barrel
Catalytic Nucleophile/Base	Asp (experimental)
Catalytic Proton Donor	Glu (experimental)
Note	New: many members have been assigned to subfamilies as described by Stam et al. (2006) Protein Eng Des Sel. 19, 555-562 (PMID: 17085431)
External resources	CAZypedia; EBI Protein of the Month; HOMSTRAD; PDB Molecule of the Month; PRINTS;
Commercial Enzyme Provider(s)	MEGAZYME; PROZOMIX;
Statistics	GenBank accession (440); Uniprot accession (26); PDB accession (11); 3D entries (1); cryst (0)

Summary

Download GH13_32 (1948) Taxonomic display Structure (1) Characterized (25)


Bacteria
Protein Name	EC#	Reference	Organism	GenBank	Uniprot	PDB/3D	Subf
α-amylase (AmyA)	3.2.1.1	pubmed	Aeromonas hydrophila MCC-1	AAA21016.1	P41131		32
α-amylase (BAA)	3.2.1.1	pubmed	Bacillus sp. (in: firmicutes) 195	BAA22082.1	O24781		32
bifunctional amylopullulanase (ApuB;Bbr_0123)	3.2.1.1		Bifidobacterium breve UCC2003	AAY89038.1 ABE94835.1	F9Y041 Q0R5Z4		32
maltotriose-forming α-amylase	3.2.1.1 3.2.1.116	pubmed pubmed	Brachybacterium sp. LB25	BAE94180.1	Q1JUA3		32
amylopullulanase (HMPREF1573_00831)	3.2.1.1		Gardnerella vaginalis JCP7276	EPI56559.1			32
α-amylase (AmyH)	3.2.1.1		Halomonas meridiana DSM 5425	CAB92963.1	Q9L4I9		32
α-amylase (Amy;KVA)	3.2.1.1	pubmed	Kocuria varians ATCC 21971	BAJ52728.1	E5RKQ5		32
α-amylase (AmyA;MaAmyA)	3.2.1.1	pubmed	Microbacterium aurum B8A	AKG25402.1	A0A0G2T4B5		32
α-amylase	3.2.1.1	URL	Pseudoalteromonas haloplanktis ANT/505	AAO72321.1	Q84CG0		32
α-amylase (Amy;AHA)	3.2.1.1	pubmed	Pseudoalteromonas haloplanktis TAB23 / A23	CAA41481.1	P29957	1AQH[A] 1AQM[A] 1B0I[A] 1G94[A] 1G9H[A] 1JD7[A] 1JD9[A] 1KXH[A] 1L0P[A] 8CQF[A] 8CQG[A]	32
α-amylase 2 (Amy2)	3.2.1.1	doi	Pseudomonas sp. KFCC10818	AAA86835.1	Q52413		32
α-amylase 4 (Amy4)	3.2.1.1		Pseudomonas sp. KFCC10818	AAK17994.1	Q9AGG4		32
α-amylase	3.2.1.1		Streptomyces albus	AAA96317.1	Q53633		32
α-amylase (SAV5981)	3.2.1.1		Streptomyces avermitilis MA-4680 = NBRC 14893	BAC73693.1 NP_827158.1	Q82AS5		32
α-amylase (Amy)	3.2.1.1		Streptomyces griseus IMRU3570	CAA40798.1	P30270		32
α-amylase	3.2.1.1		Streptomyces hygroscopicus	AAA26698.1	P08486		32
α-amylase (Aml)	3.2.1.1		Streptomyces limosus ATCC 19778	AAA88554.1	P09794		32
α-amylase (Aml;AmlA)	3.2.1.1		Streptomyces lividans TK21	CAA73926.1	O86876		32
α-amylase (AmlB)	3.2.1.1	pubmed	Streptomyces lividans TK24	CAB06622.1	P97179		32
α-amylase	3.2.1.1		Streptomyces sp. TO1	CAA73775.1	O65947		32
α-amylase (Amy)	3.2.1.1		Streptomyces sp. WL6	AAA82875.1	Q59964		32
α-amylase	3.2.1.1		Streptomyces thermoviolaceus CUB74	AAA26697.1	P27350		32
α-amylase (Aml)	3.2.1.1	pubmed	Streptomyces venezuelae ATCC 15068	AAB36561.1 QES22595.1	A0A5P2AWP4 P22998		32
maltotriose-producing α-amylase (Tfa;Amy13)	3.2.1.1 3.2.1.116		Thermobifida fusca NTU22	ABF13430.1	Q1KLC8		32
α-amylase (Tam)	3.2.1.1		Thermomonospora curvata	CAA41881.1	P29750		32
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