Glycoside Hydrolase Family 13 / Subf 32

Activities in Familyα-amylase (EC 3.2.1.1); pullulanase (EC 3.2.1.41); cyclomaltodextrin glucanotransferase (EC 2.4.1.19); cyclomaltodextrinase (EC 3.2.1.54); trehalose-6-phosphate hydrolase (EC 3.2.1.93); oligo-α-glucosidase (EC 3.2.1.10); maltogenic amylase (EC 3.2.1.133); neopullulanase (EC 3.2.1.135); α-glucosidase (EC 3.2.1.20); maltotetraose-forming α-amylase (EC 3.2.1.60); isoamylase (EC 3.2.1.68); glucodextranase (EC 3.2.1.70); maltohexaose-forming α-amylase (EC 3.2.1.98); maltotriose-forming α-amylase (EC 3.2.1.116); branching enzyme (EC 2.4.1.18); trehalose synthase (EC 5.4.99.16); 4-α-glucanotransferase (EC 2.4.1.25); maltopentaose-forming α-amylase (EC 3.2.1.-) ; amylosucrase (EC 2.4.1.4) ; sucrose phosphorylase (EC 2.4.1.7); malto-oligosyltrehalose trehalohydrolase (EC 3.2.1.141); isomaltulose synthase (EC 5.4.99.11); malto-oligosyltrehalose synthase (EC 5.4.99.15); amylo-α-1,6-glucosidase (EC 3.2.1.33); α-1,4-glucan: phosphate α-maltosyltransferase (EC 2.4.99.16); 6'-P-sucrose phosphorylase (EC 2.4.1.-); amino acid transporter
Activities in Sub Family
Mechanism Retaining
ClanGH-H
3D Structure Status( β / α ) 8
Catalytic Nucleophile/BaseAsp (experimental)
Catalytic Proton DonorGlu (experimental)
NoteNew: many members have been assigned to subfamilies as described by Stam et al. (2006) Protein Eng Des Sel. 19, 555-562 (PMID: 17085431)
External resourcesCAZypedia; EBI Protein of the Month; HOMSTRAD; PDB Molecule of the Month; PRINTS;
Commercial Enzyme Provider(s)MEGAZYME; PROZOMIX;
Statistics GenBank accession (393); Uniprot accession (76); PDB accession (9); 3D entries (1); cryst (0)
Bacteria
Protein Name EC#OrganismGenBank UniprotPDB/3DSubf
 α-amylase (AmyA) 3.2.1.1 Aeromonas hydrophila MCC-1 AAA21016.1 P41131   32
 α-amylase (BAA) 3.2.1.1 Bacillus sp. 195 BAA22082.1 O24781   32
 bifunctional amylopullulanase (ApuB;Bbr_0123) 3.2.1.1
3.2.1.41
Bifidobacterium breve UCC2003 AAY89038.1
ABE94835.1
F9Y041   32
 maltotriose-forming α-amylase 3.2.1.1
3.2.1.116
Brachybacterium sp. LB25 BAE94180.1 Q1JUA3   32
 α-amylase (AmyH) 3.2.1.1 Halomonas meridiana DSM 5425 CAB92963.1 Q9L4I9   32
 α-amylase (Amy;KVA) 3.2.1.1 Kocuria varians ATCC 21971 BAJ52728.1     32
 α-amylase (AmyA;MaAmyA) 3.2.1.1 Microbacterium aurum B8A AKG25402.1     32
 α-amylase 3.2.1.1 Pseudoalteromonas haloplanktis ANT/505 AAO72321.1 Q84CG0   32
 α-amylase (Amy;AHA) 3.2.1.1 Pseudoalteromonas haloplanktis TAB23 / A23 CAA41481.1 P29957 1AQH[A]
1AQM[A]
1B0I[A]
1G94[A]
1G9H[A]
1JD7[A]
1JD9[A]
1KXH[A]
1L0P[A]
32
 α-amylase 2 (Amy2) 3.2.1.1 Pseudomonas sp. KFCC10818 AAA86835.1 Q52413   32
 α-amylase 4 (Amy4) 3.2.1.1 Pseudomonas sp. KFCC10818 AAK17994.1 Q9AGG4   32
 α-amylase 3.2.1.1 Streptomyces albus AAA96317.1 Q53633   32
 α-amylase (SAV5981) 3.2.1.1 Streptomyces avermitilis MA-4680 = NBRC 14893 BAC73693.1
NP_827158.1
    32
 α-amylase (Amy) 3.2.1.1 Streptomyces griseus IMRU3570 CAA40798.1 P30270   32
 α-amylase 3.2.1.1 Streptomyces hygroscopicus AAA26698.1 P08486   32
 α-amylase (Aml) 3.2.1.1 Streptomyces limosus ATCC 19778 AAA88554.1 P09794   32
 α-amylase (Aml;AmlA) 3.2.1.1 Streptomyces lividans TK21 CAA73926.1 O86876   32
 α-amylase (AmlB) 3.2.1.1 Streptomyces lividans TK24 CAB06622.1 P97179   32
 α-amylase 3.2.1.1 Streptomyces sp. TO1 CAA73775.1 O65947   32
 α-amylase (Amy) 3.2.1.1 Streptomyces sp. WL6 AAA82875.1 Q59964   32
 α-amylase 3.2.1.1 Streptomyces thermoviolaceus CUB74 AAA26697.1 P27350   32
 α-amylase (Aml) 3.2.1.1 Streptomyces venezuelae ATCC 15068 AAB36561.1 P22998   32
 maltotriose-producing α-amylase (Tfa;Amy13) 3.2.1.1
3.2.1.116
Thermobifida fusca NTU22 ABF13430.1 Q1KLC8   32
 α-amylase (Tam) 3.2.1.1 Thermomonospora curvata CAA41881.1 P29750   32
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