Activities in Family	endo-β-1,4-mannanase (EC 3.2.1.78);
Mechanism	inverting
3D Structure Status	lysozyme fold
Note	Created after the work by Shimizu and collaborators. J. Biol. Chem. (2015). [ PMID:26385921 ]. Presents an inverting mechanism in what is a unique feature compared to all other b-mannanases [see Jin et al. ACS Cent. Sci (2016) doi:10.1021/acscentsci.6b00232 ]
External resources	CAZypedia;
Statistics	GenBank accession (155); Uniprot accession (0); PDB accession (9); 3D entries (2); cryst (0)

Bacteria
Protein Name	EC#	Organism	GenBank	Uniprot	PDB/3D Carbohydrate Ligands Resolution (Å)
β-1,4-mannanase (SsGH134)	3.2.1.78	Streptomyces sp. NRRL B-24484	WP_030268297.1		5JTS[A] 2.27 5JU9[A] β-D-Manp-(1-4)-β-D-Manp-(1-4)-α-D-Manp 2.10 5JUG[A] β-D-Manp-(1-4)-β-D-Manp-(1-4)-β-D-Manp-(1-4)-β-D-Manp-(1-4)-β-D-Manp 2.05
Eukaryota
Protein Name	EC#	Organism	GenBank	Uniprot	PDB/3D Carbohydrate Ligands Resolution (Å)
RMCBS344292_04886		Rhizopus microsporus	CEI90564.1		5XTJ[A,B] 1.50 5XTT[A,B] β-D-Manp-(1-4)-β-D-Manp-(1-4)-β-D-Manp NMR 5XU5[A,B] β-D-Manp-(1-4)-β-D-Manp-(1-4)-β-D-Manp-(1-4)-β-D-Manp 1.39 5XUG[A,B] β-D-Manp-(1-4)-β-D-Manp-(1-4)-β-D-Manp β-D-Manp-(1-4)-β-D-Manp-(1-4)-β-D-Manp-(1-4)-β-D-Manp-(1-4)-β-D-Manp 2.25 5XUL[A,B] β-D-Manp-(1-4)-β-D-Manp-(1-4)-β-D-Manp-(1-4)-β-D-Manp-(1-4)-β-D-Manp-(1-4)-β-D-Manp 1.30 5XXA[A,B] 2.40
Top

Last update: 2021-03-26 © Copyright 1998-2021
AFMB - CNRS - Université d'Aix-Marseille