Activities in Sub Family	Endo-β-1,4-mannanase (EC 3.2.1.78);
Mechanism	Retaining e
Clan	GH-A
3D Structure Status	( β / α ) 8 barrel
Catalytic Nucleophile/Base	Glu (experimental)
Catalytic Proton Donor	Glu (experimental)
Note	Once known as cellulase family A; many members have been assigned to subfamilies as described by Aspeborg et al. (2012) BMC Evol Biol. 12(1):186 (PMID: 22992189).
External resources	CAZypedia; HOMSTRAD; PROSITE;
Commercial Enzyme Provider(s)	MEGAZYME; NZYTech; PROZOMIX;
Statistics	GenBank accession (115); Uniprot accession (4); PDB accession (4); 3D entries (2); cryst (0)

Archaea
Protein Name	EC#	Organism	GenBank	Uniprot	PDB/3D	Subf
I7X12_14415		Halosimplex litoreum YGH94	QPV61938.1			36
HZS54_25460		Halosimplex pelagicum R2	QLH84787.1			36
HZS55_21965		Halosimplex rubrum R27	QLH79797.1			36
Bacteria
Protein Name	EC#	Organism	GenBank	Uniprot	PDB/3D	Subf
ACPOL_3508		Acidisarcina polymorpha SBC82	AXC12793.1			36
HUW48_25635		Adhaeribacter radiodurans KUDC8001	QMU31199.1			36
HUW51_09125		Adhaeribacter swui KCTC 52873	QNF32886.1			36
SLW71_14695		Algoriphagus sp. NG3	WPR73921.1			36
OM944_16135		Algoriphagus sp. TR-M5	UZD22190.1			36
PBT90_01810		Algoriphagus sp. TR-M9	WBL43441.1			36
FRZ59_17525		Anseongella ginsenosidimutans Gsoil 524	QEC53950.1			36
SAMN00777080_1055		Aquiflexum balticum DSM 16537	SMD42501.1			36
AQPE_4796		Aquipluma nitroreducens MeG22	BBE20602.1			36
DJ013_04540		Arcticibacterium luteifluviistationis SM1504	AWV97476.1			36
Q31a_20890 (fragment)		Aureliella helgolandensis Q31a	QDV23784.1			36
SAMN05444321_7907		Bradyrhizobium lablabi MT34	SHM85288.1			36
SAMN05444163_6030		Bradyrhizobium ottawaense GAS524	SDJ64628.1			36
CalpoDRAFT_1214 (Man5B)		Caldanaerobius polysaccharolyticus ATCC BAA-17	WP_026486535.1			36
endomannanase / endoglucanase (ManB) (Man5B)	3.2.1.78	Caldanaerobius polysaccharolyticus KMTHCJ	AGA35556.1 ADK22147.1	D9J0D7	3W0K[A,B]	36
Calla_2118		Caldicellulosiruptor acetigenus 6A	AEM74681.1			36
OTK01_002405		Caldicellulosiruptor acetigenus DSM 7040	WAM36024.1			36
Calkr_0231 (Cel14)		Caldicellulosiruptor acetigenus I77R1B	ADQ39796.1			36
Athe_0234		Caldicellulosiruptor bescii DSM 6725	ACM59384.1			36
ELD05_00720		Caldicellulosiruptor changbaiensis CBS-Z	AZT89324.1			36
CaldiYA01_21590		Caldicellulosiruptor diazotrophicus YA01	BCS82199.1			36
Calhy_2367		Caldicellulosiruptor hydrothermalis 108	ADQ08066.1			36
Calkro_2344		Caldicellulosiruptor kronotskyensis 2002	ADQ47167.1			36
OTK00_000091		Caldicellulosiruptor morganii DSM 8990	WAM33949.1			36
OTJ99_000097		Caldicellulosiruptor naganoensis DSM 8991	WAM31666.1			36
Csac_0137		Caldicellulosiruptor saccharolyticus DSM 8903	ABP65789.1			36
Q2T83_05685		Candidatus Fervidibacter sacchari PD1	WKU17313.1			36
PZB72_02085		Catalinimonas niigatensis NBRC 109829	WPP53736.1			36
FW415_11655		Chitinophaga sp. XS-30	QEH41502.1			36
QNM32_24600		Chitinophagaceae bacterium DXS	WJK46920.1			36
D4L85_09610		Chryseolinea soli KIS68-18	AYB35478.1			36
CA2015_1573		Cyclobacterium amurskyense KCTC 12363	AKP51009.1			36
Cycma_1666		Cyclobacterium marinum DSM 745	AEL25420.1			36
LAG90_19485		Cytophagaceae bacterium YYF0007	UBM58985.1			36
Daura_15785		Dactylosporangium aurantiacum NRRL B-8018	UWZ57481.1			36
N0D28_00025		Deinococcus rubellus Ant6	UWX64104.1			36
DICTH_0508		Dictyoglomus thermophilum H-6-12	ACI19118.1			36
endo-β-1,4-mannanase (CelB; DturCelB; Dtur_0671)	3.2.1.78	Dictyoglomus turgidum DSM 6724	ACK41956.1	B8DZM3		36
G0Q07_13320		Draconibacterium halophilum M1	QIA08636.1			36
FH5T_22070		Draconibacterium orientale FH5	AHW61392.1			36
NFI80_09810		Dyadobacter chenhuakuii CY22	USJ33029.1			36
MUK70_02600		Dyadobacter chenwenxiniae LJ419	UON83884.1			36
NFI81_01930		Dyadobacter fanqingshengii CY399	USJ36536.1			36
Dfer_0289		Dyadobacter fermentans DSM 18053	ACT91559.1			36
ON006_07200		Dyadobacter pollutisoli U1	WAC13736.1			36
HWI92_01455		Dyadobacter sandarakinus Q3-56	QRQ99672.1			36
ING2E5B_1419		Fermentimonas caenicola ING2-E5B	CEA16167.1			36
FAES_0109		Fibrella aestuarina BUZ 2	CCG98123.1			36
OP10G_2788		Fimbriimonas ginsengisoli Gsoil 348	AIE86156.1			36
R9C00_02880		Flammeovirgaceae bacterium SG7u.111	WPO36385.1			36
NXS98_05300 (fragment)		Fontisphaera persica B-154	WCJ60547.1			36
Haur_0888		Herpetosiphon aurantiacus DSM 785	ABX03536.1			36
OF852_11430		Homoserinibacter sp. YIM 151385	WBU37520.1			36
RT717_04770		Imperialibacter roseus P4T	WOK07941.1			36
Verru16b_01397		Lacunisphaera limnophila IG16b	AOS44336.1			36
Verru16b_01855		Lacunisphaera limnophila IG16b	AOS44786.1			36
Verru16b_02550		Lacunisphaera limnophila IG16b	AOS45469.1			36
SAMN04515683_3551		Leifsonia sp. 157MF	SDK46852.1			36
SAMN04515690_0569		Leifsonia sp. 197AMF	SDH07135.1			36
SAMN04515684_3214		Leifsonia sp. 466MF	SDJ33083.1			36
SAMN04515685_3533		Leifsonia sp. 467MF	SEN55982.1			36
SAMN04515686_1399		Leifsonia sp. 509MF	SDN54077.1			36
SAMN04515691_3448		Leifsonia sp. 98AMF	SFM73591.1			36
Mahau_2106		Mahella australiensis 50-1 BON	AEE97278.1			36
GM418_00660		Maribellus comscasis WC007	QGY42217.1			36
MROS_2625		Melioribacter roseus P3M-2	AFN75855.1			36
Niako_5856		Niastella koreensis GR20-10	AEW02087.1			36
ASA1KI_36060		Opitutales bacterium ASA1	BET68688.1			36
ASA1KI_36060		Opitutales bacterium ASA1	BET68688.1			36
ASA1KI_02430		Opitutales bacterium ASA1	BET65325.1			36
Oter_0119		Opitutus terrae PB90-1	ACB73410.1			36
Oter_0987		Opitutus terrae PB90-1	ACB74275.1			36
FRZ67_20170		Panacibacter ginsenosidivorans Gsoil1550	QEC69503.1			36
NXY22_12370		Parabacteroides distasonis BFG-158	UVR24227.1			36
CPT03_05930		Pedobacter ginsengisoli T01R-27	ATP56029.1			36
ING2E5A_0461 (CelC)		Petrimonas mucosa DSM 28695	SCM55578.1			36
HED60_16755		Planctomycetales bacterium zrk34	QNN23848.1			36
GXP67_23460		Rhodocytophaga rosea 172606-1	QHT69385.1			36
AWN76_009395		Rhodothermaceae bacterium RA	ARA93352.1			36
K4G66_08710		Tunicatimonas sp. TK19036	WKN40326.1			36
DR864_21405		Runella rosea HYN0085	AXE20122.1			36
Runsl_2570		Runella slithyformis DSM 19594	AEI48971.1			36
STHERM_c07100		Spirochaeta thermophila DSM 6192	ADN01668.1			36
Spith_1446		Spirochaeta thermophila DSM 6578	AEJ61710.1			36
H3H32_21230		Spirosoma foliorum KCTC 72228	QMW00519.1			36
Slin_3338		Spirosoma linguale DSM 74	ADB39348.1			36
AWR27_14240		Spirosoma montaniterrae DY10	AQG82496.1			36
LQ777_18385		Spirosoma oryzicola RHs26	UHG90210.1			36
CWM47_18970		Spirosoma pollinicola HA7	AUD03727.1			36
SD10_07045		Spirosoma radiotolerans DG5A	AKD54702.1			36
EXU85_07150		Spirosoma sp. KCTC 42546	QDK78392.1			36
LN737_27235		Spirosoma sp. KNUC1025	UFH54016.1			36
HU175_19110		Spirosoma sp. KUDC1026	QKZ14620.1			36
WBJ53_18860		Spirosoma sp. SC4-14	WXC13043.1			36
HNV11_06690		Spirosoma taeanense TS118	QJW89098.1			36
Tsac_2327		Thermoanaerobacterium saccharolyticum JW/SL-YS485	AFK87329.1			36
Q2T46_03895		Thermoanaerobacterium sp. CMT5567-10	WKV09605.1			36
SAMN05660242_0042		Thermoanaerobacterium sp. RBIITD	SNX52631.1			36
Tthe_1935		Thermoanaerobacterium thermosaccharolyticum DSM 571	ADL69420.1			36
PGH24_09800		Thermoanaerobacterium thermosaccharolyticum MJ1	WHE06455.1			36
IMW88_10055		Thermoflavifilum sp. isolate Bu77	QOR75661.1			36
THTE_3688		Thermogutta terrifontis R1	ASV76289.1			36
endo-mannanase (Man/Cel5B; TM1752;TM_1752) (Cel5B)	3.2.1.78	Thermotoga maritima MSB8	AAD36817.1 AGL50684.1 AHD18354.1 AKE29507.1 NP_229550.1	Q9X274 R4P1V9	1VJZ[A] 7EC9[A,B] 7EFZ[A,B]	36
THMB_1794		Thermotoga maritima Tma100	AKE31378.1			36
THMC_1794		Thermotoga maritima Tma200	AKE27634.1			36
TRQ2_1073		Thermotoga sp. RQ2	ACB09419.1			36
Trebr_1919		Treponema brennaborense DSM 12168	AEE17338.1			36
P0M28_26790		Tunicatimonas pelagia NBRC 107804	WKN42650.1			36
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