Glycoside Hydrolase Family 5 / Subf 36

Activities in Familyendo-β-1,4-glucanase / cellulase (EC 3.2.1.4); endo-β-1,4-xylanase (EC 3.2.1.8); β-glucosidase (EC 3.2.1.21); β-mannosidase (EC 3.2.1.25); β-glucosylceramidase (EC 3.2.1.45); glucan β-1,3-glucosidase (EC 3.2.1.58); licheninase (EC 3.2.1.73); exo-β-1,4-glucanase / cellodextrinase (EC 3.2.1.74); glucan endo-1,6-β-glucosidase (EC 3.2.1.75); mannan endo-β-1,4-mannosidase (EC 3.2.1.78); cellulose β-1,4-cellobiosidase (EC 3.2.1.91); steryl β-glucosidase (EC 3.2.1.104); endoglycoceramidase (EC 3.2.1.123); chitosanase (EC 3.2.1.132); β-primeverosidase (EC 3.2.1.149); xyloglucan-specific endo-β-1,4-glucanase (EC 3.2.1.151); endo-β-1,6-galactanase (EC 3.2.1.164); hesperidin 6-O-α-L-rhamnosyl-β-glucosidase (EC 3.2.1.168); β-1,3-mannanase (EC 3.2.1.-); arabinoxylan-specific endo-β-1,4-xylanase (EC 3.2.1.-); mannan transglycosylase (EC 2.4.1.-)
Activities in Sub Family
Mechanism Retaining
ClanGH-A
3D Structure Status( β / α ) 8
Catalytic Nucleophile/BaseGlu (experimental)
Catalytic Proton DonorGlu (experimental)
NoteOnce known as cellulase family A; many members have been assigned to subfamilies as described by Aspeborg et al. (2012) BMC Evol Biol. 12(1):186 (PMID: 22992189).
External resourcesCAZypedia; HOMSTRAD; PROSITE;
Commercial Enzyme Provider(s)MEGAZYME; NZYTech; PROZOMIX;
Statistics GenBank accession (57); Uniprot accession (17); PDB accession (2); 3D entries (2); cryst (0)
Bacteria
Protein Name EC#Organism GenBankUniprotPDB/3DSubf
 SAMN00777080_1055   Aquiflexum balticum DSM 16537 SMD42501.1     36
 SAMN05444321_7907   Bradyrhizobium lablabi MT34 SHM85288.1     36
 SAMN05444163_6030   Bradyrhizobium ottawaense GAS524 SDJ64628.1     36
 CalpoDRAFT_1214 (Man5B)   Caldanaerobius polysaccharolyticus ATCC BAA-17 WP_026486535.1     36
 endomannanase / endoglucanase (ManB) (Man5B) 3.2.1.78 Caldanaerobius polysaccharolyticus KMTHCJ AGA35556.1
ADK22147.1
D9J0D7 3W0K[A,B] 36
 Athe_0234   Caldicellulosiruptor bescii DSM 6725 ACM59384.1 B9MMF2   36
 Calhy_2367   Caldicellulosiruptor hydrothermalis 108 ADQ08066.1 E4Q8U2   36
 Calkr_0231 (Cel14)   Caldicellulosiruptor kristjanssonii I77R1B ADQ39796.1 E4S721   36
 Calkro_2344   Caldicellulosiruptor kronotskyensis 2002 ADQ47167.1 E4SHE2   36
 Calla_2118   Caldicellulosiruptor lactoaceticus 6A AEM74681.1     36
 Csac_0137   Caldicellulosiruptor saccharolyticus DSM 8903 ABP65789.1 A4XFV4   36
 CA2015_1573   Cyclobacterium amurskyense KCTC 12363 AKP51009.1     36
 Cycma_1666   Cyclobacterium marinum DSM 745 AEL25420.1     36
 DICTH_0508   Dictyoglomus thermophilum H-6-12 ACI19118.1 B5YCY1   36
 endoglucanase (Dtur_0671) 3.2.1.- Dictyoglomus turgidum DSM 6724 ACK41956.1 B8DZM3   36
 FH5T_22070   Draconibacterium orientale FH5 AHW61392.1     36
 Dfer_0289   Dyadobacter fermentans DSM 18053 ACT91559.1 C6VX86   36
 ING2E5B_1419   Fermentimonas caenicola CEA16167.1     36
 FAES_0109   Fibrella aestuarina BUZ 2 CCG98123.1     36
 OP10G_2788   Fimbriimonas ginsengisoli Gsoil 348 AIE86156.1     36
 Haur_0888   Herpetosiphon aurantiacus DSM 785 ABX03536.1 A9AYF4   36
 Verru16b_01397   Lacunisphaera limnophila IG16b AOS44336.1     36
 Verru16b_01855   Lacunisphaera limnophila IG16b AOS44786.1     36
 Verru16b_02550   Lacunisphaera limnophila IG16b AOS45469.1     36
 SAMN04515683_3551   Leifsonia sp. 157MF SDK46852.1     36
 SAMN04515690_0569   Leifsonia sp. 197AMF SDH07135.1     36
 SAMN04515684_3214   Leifsonia sp. 466MF SDJ33083.1     36
 SAMN04515685_3533   Leifsonia sp. 467MF SEN55982.1     36
 SAMN04515686_1399   Leifsonia sp. 509MF SDN54077.1     36
 SAMN04515691_3448   Leifsonia sp. 98AMF SFM73591.1     36
 Mahau_2106   Mahella australiensis 50-1 BON AEE97278.1     36
 MROS_2625   Melioribacter roseus P3M-2 AFN75855.1     36
 Niako_5856   Niastella koreensis GR20-10 AEW02087.1     36
 Oter_0119   Opitutus terrae PB90-1 ACB73410.1 B1ZMF7   36
 Oter_0987   Opitutus terrae PB90-1 ACB74275.1 B1ZXQ3   36
 ING2E5A_0461 (CelC)   Petrimonas mucosa DSM 28695 SCM55578.1     36
 AWN76_009395   Rhodothermaceae bacterium RA ARA93352.1     36
 Runsl_2570   Runella slithyformis DSM 19594 AEI48971.1     36
 STHERM_c07100   Spirochaeta thermophila DSM 6192 ADN01668.1 E0RRG7   36
 Spith_1446   Spirochaeta thermophila DSM 6578 AEJ61710.1     36
 Slin_3338   Spirosoma linguale DSM 74 ADB39348.1 D2QNF9   36
 AWR27_14240   Spirosoma montaniterrae DY10 AQG82496.1     36
 SD10_07045   Spirosoma radiotolerans DG5A AKD54702.1     36
 Tsac_2327   Thermoanaerobacterium saccharolyticum JW/SL-YS485 AFK87329.1     36
 SAMN05660242_0042   Thermoanaerobacterium sp. RBIITD SNX52631.1     36
 Tthe_1935   Thermoanaerobacterium thermosaccharolyticum DSM 571 ADL69420.1 D9TTG1   36
 THTE_3688   Thermogutta terrifontis R1 ASV76289.1     36
 lichenase / endo-mannanase (TM1752;TM_1752) (Cel5B) 3.2.1.73
3.2.1.78
Thermotoga maritima MSB8 AAD36817.1
AGL50684.1
AHD18354.1
AKE29507.1
NP_229550.1
Q9X274 1VJZ[A] 36
 THMB_1794   Thermotoga maritima Tma100 AKE31378.1     36
 THMC_1794   Thermotoga maritima Tma200 AKE27634.1     36
 TRQ2_1073   Thermotoga sp. RQ2 ACB09419.1 B1LAS1   36
 Trebr_1919   Treponema brennaborense DSM 12168 AEE17338.1     36
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