Glycoside Hydrolase Family 5 / Subf 23

Activities in Familyendo-β-1,4-glucanase / cellulase (EC 3.2.1.4); endo-β-1,4-xylanase (EC 3.2.1.8); β-glucosidase (EC 3.2.1.21); β-mannosidase (EC 3.2.1.25); β-glucosylceramidase (EC 3.2.1.45); glucan β-1,3-glucosidase (EC 3.2.1.58); licheninase (EC 3.2.1.73); exo-β-1,4-glucanase / cellodextrinase (EC 3.2.1.74); glucan endo-1,6-β-glucosidase (EC 3.2.1.75); mannan endo-β-1,4-mannosidase (EC 3.2.1.78); cellulose β-1,4-cellobiosidase (EC 3.2.1.91); steryl β-glucosidase (EC 3.2.1.104); endoglycoceramidase (EC 3.2.1.123); chitosanase (EC 3.2.1.132); β-primeverosidase (EC 3.2.1.149); xyloglucan-specific endo-β-1,4-glucanase (EC 3.2.1.151); endo-β-1,6-galactanase (EC 3.2.1.164); hesperidin 6-O-α-L-rhamnosyl-β-glucosidase (EC 3.2.1.168); β-1,3-mannanase (EC 3.2.1.-); arabinoxylan-specific endo-β-1,4-xylanase (EC 3.2.1.-); mannan transglycosylase (EC 2.4.1.-)
Activities in Sub Family
Mechanism Retaining
ClanGH-A
3D Structure Status( β / α ) 8
Catalytic Nucleophile/BaseGlu (experimental)
Catalytic Proton DonorGlu (experimental)
NoteOnce known as cellulase family A; many members have been assigned to subfamilies as described by Aspeborg et al. (2012) BMC Evol Biol. 12(1):186 (PMID: 22992189).
External resourcesCAZypedia; HOMSTRAD; PROSITE;
Commercial Enzyme Provider(s)MEGAZYME; NZYTech; PROZOMIX;
Statistics GenBank accession (11); Uniprot accession (7);
Eukaryota
Protein Name EC#Organism GenBankUniprotPDB/3DSubf
 β-rutinosidase / α-L-rhamnosyl-β-glucosidase   Acremonium sp. DSM 24697 SES201 AMD11613.1     23
 AN1332.2   Aspergillus nidulans FGSC A4 EAA65515.1 C8VS48
Q5BDP8
  23
 An06g02060   Aspergillus niger CBS 513.88 CAK48049.1 A2QLQ4   23
 An08g01100   Aspergillus niger CBS 513.88 CAK39791.1 A2QQ32   23
 AO090012000917   Aspergillus oryzae RIB40 BAE61018.1 Q2UBP7   23
 glycoside hydrolase family 5 protein (Bofut4_p065480.1)   Botrytis cinerea T4 CCD33736.1     23
 FFUJ_02196   Fusarium fujikuroi IMI 58289 CCT65263.1     23
 FFUJ_03742   Fusarium fujikuroi IMI 58289 CCT64988.1     23
 unnamed protein product   Fusarium graminearum PH-1 CEF85699.1     23
 β-primeverosidase (Bgl2) 3.2.1.149 Penicillium multicolor TS-5 / IAM7153 BAG70961.1 B5MEI8   23
 Pc13g14840   Penicillium rubens Wisconsin 54-1255 CAP92553.1 B6H2P2   23
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