Glycoside Hydrolase Family 5 / Subf 14

Activities in Familyendo-β-1,4-glucanase / cellulase (EC 3.2.1.4); endo-β-1,4-xylanase (EC 3.2.1.8); β-glucosidase (EC 3.2.1.21); β-mannosidase (EC 3.2.1.25); β-glucosylceramidase (EC 3.2.1.45); glucan β-1,3-glucosidase (EC 3.2.1.58); licheninase (EC 3.2.1.73); exo-β-1,4-glucanase / cellodextrinase (EC 3.2.1.74); glucan endo-1,6-β-glucosidase (EC 3.2.1.75); mannan endo-β-1,4-mannosidase (EC 3.2.1.78); cellulose β-1,4-cellobiosidase (EC 3.2.1.91); steryl β-glucosidase (EC 3.2.1.104); endoglycoceramidase (EC 3.2.1.123); chitosanase (EC 3.2.1.132); β-primeverosidase (EC 3.2.1.149); xyloglucan-specific endo-β-1,4-glucanase (EC 3.2.1.151); endo-β-1,6-galactanase (EC 3.2.1.164); hesperidin 6-O-α-L-rhamnosyl-β-glucosidase (EC 3.2.1.168); β-1,3-mannanase (EC 3.2.1.-); arabinoxylan-specific endo-β-1,4-xylanase (EC 3.2.1.-); mannan transglycosylase (EC 2.4.1.-)
Activities in Sub Family
Mechanism Retaining
ClanGH-A
3D Structure Status( β / α ) 8
Catalytic Nucleophile/BaseGlu (experimental)
Catalytic Proton DonorGlu (experimental)
NoteOnce known as cellulase family A; many members have been assigned to subfamilies as described by Aspeborg et al. (2012) BMC Evol Biol. 12(1):186 (PMID: 22992189).
External resourcesCAZypedia; HOMSTRAD; PROSITE;
Commercial Enzyme Provider(s)MEGAZYME; NZYTech; PROZOMIX;
Statistics GenBank accession (52); Uniprot accession (9);
Eukaryota
Protein Name EC#Organism GenBankUniprotPDB/3DSubf
 β-glucosidase 1 GH5 family (GH5BG1;CsGH5BG1) 3.2.1.21 Camellia sinensis ARU79086.1     14
 β-glucosidase 2 GH5 family (Gh5bg2)   Camellia sinensis ARU79087.1     14
 β-glucosidase 3 GH5 family (Gh5bg3)   Camellia sinensis ARU79088.1     14
 glucan 1,3-β-glucosidase   Drosera adelae BAW35423.1     14
 predicted protein   Hordeum vulgare subsp. vulgare BAJ97814.1     14
 predicted protein   Hordeum vulgare subsp. vulgare BAK05376.1     14
 predicted protein (fragment)   Hordeum vulgare subsp. vulgare BAK07818.1     14
 ORF (fragment)   Hordeum vulgare subsp. vulgare AGW47737.1     14
 unknown   Lotus japonicus AFK35120.1     14
 unknown (fragment)   Lotus japonicus AFK38496.1     14
 unknown   Medicago truncatula AFK33638.1     14
 unknown   Medicago truncatula AFK42423.1     14
 MtrDRAFT_AC145165g35v2   Medicago truncatula ABP02833.1
ABE91798.1
ABE91799.1
    14
 MtrDRAFT_AC147959g9v1   Medicago truncatula ABP03047.1
AFK37837.1
    14
 MtrDRAFT_AC147959g24v1   Medicago truncatula ABP03049.1     14
 MtrDRAFT_AC147959g10v1   Medicago truncatula ABP03050.1     14
 exo-β-1,3-glucosidase (GH5BG;Os10g0370500) 3.2.1.58 Oryza sativa Japonica Group AAM08614.1
AAM08821.1
AAP53379.1
BAF26372.1
BAG89316.1
BAT10560.1
Q8RU06   14
 Os10g0370800   Oryza sativa Japonica Group AAM08620.1
AAP53385.1
BAF26375.1
BAT10564.1
NP_001064461.1
Q8RU51   14
 Os10g0370800   Oryza sativa Japonica Group AAM08620.1
AAP53385.1
BAF26375.1
BAT10564.1
NP_001064461.1
Q8RU51   14
 Os10g0370800   Oryza sativa Japonica Group AAM08620.1
AAP53385.1
BAF26375.1
BAT10564.1
BAT10565.1
NP_001064461.1
Q8RU51   14
 Os08g0484100   Oryza sativa Japonica Group BAD10703.1
BAD09304.1
BAF23993.1
Q6YTT8   14
 Os05g0244500   Oryza sativa Japonica Group AAV43969.1
BAF16925.1
BAS92988.1
Q5W708   14
 WS02732_A08   Picea sitchensis ABR16246.1 B8LKR6   14
 0_10583_01 (fragment)   Pinus taeda AFG60951.1
AFG60952.1
    14
 SHCRBa_220_E01_R_20 (fragment)   Saccharum hybrid cultivar R570 AGT17245.1     14
 88n22   Trifolium repens ADD09589.1 D3YBD8   14
 VIGAN_07175900 (Vigan.07g175900)   Vigna angularis var. angularis BAT92896.1     14
 VIGAN_03157600 (Vigan.03g157600)   Vigna angularis var. angularis BAT81736.1     14
 VIGAN_04421400 (Vigan.04g421400)   Vigna angularis var. angularis BAT86547.1     14
 VIGAN_01084100 (Vigan.01g084100)   Vigna angularis var. angularis BAT73366.1     14
 VIGAN_03157700 (Vigan.03g157700) (fragment)   Vigna angularis var. angularis BAT81737.1     14
 VITISV_000586   Vitis vinifera CAN74231.1 A5B782   14
 VITISV_016766 + VITISV_016765 (fragments)   Vitis vinifera CAN78585.1
CAN78586.1
    14
 unknown   Zea mays ACG40334.1 B6TTA1   14
 unknown (ZM_BFb0224N18) (fragment)   Zea mays B73 ACF87421.1 B4FZ78   14
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