Activities in Family	Binding to maltotriose and maltotetraose shown for the pullulanase of Anoxybacillus sp. LM18-11. Binding function derived from crystal structure and deletion of the CBM, which showed reduced specific activity and increased Km value compared to the wild type enzyme.
Note	Family created following Xu et al., Proteins 82:1685-1693 [PMID: 24375572].
Statistics	GenBank accession (1188); Uniprot accession (10); PDB accession (6); 3D entries (2); cryst (0)

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Bacteria
Protein Name	EC#	Organism	GenBank	Uniprot	PDB/3D
DK878_10765 (PulA)		Streptococcus suis ISU2660	QLL56454.1
DK876_10135 (PulA)		Streptococcus suis ISU2714	QLL52332.1
A7J09_11975		Streptococcus suis ISU2812	ASW52763.1
SSUJS14_2089		Streptococcus suis JS14	ADV71133.1
DP112_10730 (PulA)		Streptococcus suis JSHA1003	AXI68500.1
A9494_09955		Streptococcus suis LSM102	ARX91316.1
L3X06_09860 (fragment)		Streptococcus suis LSM157	UJX23053.1
GRI05_10385 (PulA)		Streptococcus suis LSM178	QOJ58064.1
L3X15_10275 (PulA)		Streptococcus suis LSM29	UJX21144.1
NLY76_10145 (PulA)		Streptococcus suis LSS42	UTI55159.1
NCTC10234_01899 (AmyX)		Streptococcus suis NCTC10234	SQH01510.1
NCTC10237_02079 (Amyx_2)		Streptococcus suis NCTC10237	VTT10116.1
K6972_10240 (PulA)		Streptococcus suis NJ3	QZS60910.1
AA105_11095		Streptococcus suis NSUI002	ALA29738.1
APQ97_11185		Streptococcus suis NSUI060	AML47565.1
SSU1920		Streptococcus suis P1/7	CAR47610.1
YYK_09255		Streptococcus suis S735	AFR01356.1
NJAUSS_1961		Streptococcus suis SC070731	AGG65417.1
JZY07_11120 (PulA)		Streptococcus suis SC183	QSQ90941.1
B9H01_10280		Streptococcus suis SC19	ARL70889.1
SSUSC84_1938		Streptococcus suis SC84	CAZ52714.1
D2A30_10330 (PulA)		Streptococcus suis SFJ44	ULL21914.1
DP111_10910 (PulA)		Streptococcus suis SH1510	AXI66462.1
A7J08_09685		Streptococcus suis SRD478	ASW50525.1
SSU12_2057		Streptococcus suis SS12	AER16232.1
BVD85_10115 (PulA)		Streptococcus suis SS2-1	AUW22887.1
K6976_10580 (PulA)		Streptococcus suis SS389	QZS51229.1
SSUST1_2032		Streptococcus suis ST1	AER22371.1
SSUST3_1971		Streptococcus suis ST3	AEB82380.1
NLY35_10950 (PulA)		Streptococcus suis STC104	UTH71082.1
NKZ79_10945 (PulA)		Streptococcus suis STC78	UTH54275.1
NKZ64_10950 (PulA)		Streptococcus suis STC80	UTH56375.1
NKZ66_10935 (PulA)		Streptococcus suis STC81	UTH58480.1
NKZ67_10950 (PulA)		Streptococcus suis STC83	UTH60581.1
NKZ65_10945 (PulA)		Streptococcus suis STC84	UTH62686.1
NKZ80_10935 (PulA)		Streptococcus suis STC85	UTH64779.1
NKZ81_10935 (PulA)		Streptococcus suis STC86	UTH66882.1
NKZ63_10950 (PulA)		Streptococcus suis STC90	UTH68985.1
K8227_10145 (PulA)		Streptococcus suis SZ1908	UAM76011.1
NLY75_10995 (PulA)		Streptococcus suis T15	UTI57214.1
T15_2193		Streptococcus suis T15	AGZ24263.1
TL13_1935		Streptococcus suis TL13	AGL48857.1
K6967_09830 (PulA)		Streptococcus suis Transconjugant cAKJ18	QZT31458.1
K6971_09830 (PulA)		Streptococcus suis Transconjugant cDY107	QZT25291.1
K6970_09805 (PulA)		Streptococcus suis Transconjugant cFJSM5	QZT21527.1
K6968_09845 (PulA)		Streptococcus suis Transconjugant cNJ3	QZT19629.1
K6975_09750 (PulA)		Streptococcus suis Transconjugant cSS389	QZT23392.1
NOV96_10725 (PulA)		Streptococcus suis WUSS030	UYZ66260.1
E8M06_10800 (PulA)		Streptococcus suis WUSS351	QCE39732.1
YB51_9770		Streptococcus suis YB51	AGW88326.1
I5W22_10230 (PulA)		Streptococcus suis YP20190405	QPO28721.1
D2E16_12450 (PulA)		Streptococcus suis YSJ17	QOZ90073.1
I5V48_11475 (PulA)		Streptococcus suis YZDH1	QPO26536.1
ZY05719_10115		Streptococcus suis ZY05719	AKG41326.1
NCTC3166_01924 (AmyX)		Streptococcus viridans NCTC3166	VED68085.1
BC6307_17220		Sutcliffiella cohnii DSM 6307	AST92903.1
B4U37_17140		Sutcliffiella horikoshii 20a	ART77673.1
K7887_16985 (PulA)		Sutcliffiella horikoshii BAT	UAL46583.1
O1A01_19960 (PulA)		Sutcliffiella sp. NC1	WBL14161.1
pullulanase (Pul)		Thermus sp. IM6501	AAC15073.1	O69008
pullulanase (Pul)		Thermus thermophilus HB8	BAB62095.1	Q7DI19
J0J70_01255 (PulA)		Turicibacter bilis ISU324	UUF08681.1
J0J70_08845 (PulA)		Turicibacter bilis ISU324	UUF07728.1
J0J69_02520 (PulA)		Turicibacter bilis MMM721	UUF06480.1
J0J69_12695 (PulA)		Turicibacter bilis MMM721	UUF05873.1
J0J80_07720 (PulA)		Turicibacter bilis PIG517	UUF09937.1
J0J80_10845 (PulA)		Turicibacter bilis PIG517	UUF10515.1
HLK68_12755 (PulA)		Turicibacter sanguinis MOL361	QJS20077.1
HLK68_09280 (PulA)		Turicibacter sanguinis MOL361	QJS19429.1
AT726_12390		Turicibacter sp. H121	AMC09634.1
AT726_09460		Turicibacter sp. H121	AMC09101.1
PersiPul1, partial (fragment)		uncultured bacterium	UQS95312.1
G7082_09930 (PulA)		Vagococcus hydrophili HDW17B	QIL48803.1
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