CAZy Family GH27

CAZy ~ Carbohydrate-Active enZymes

Families

Glycoside Hydrolases

GlycosylTransferases

Polysaccharide Lyases

Carbohydrate Esterases

Carbohydrate-Binding Modules


Glycoside Hydrolase Family 27


Known Activities	-galactosidase (EC 3.2.1.22); -N-acetylgalactosaminidase (EC 3.2.1.49); isomalto-dextranase (EC 3.2.1.94).

Mechanism	Retaining

Catalytic Nucleophile/Base	Asp

Catalytic Proton Donor	Asp

3D Structure Status	Fold: ( / ) ₈

Clan	GH-D

Note	Mechanistic commonality with family GH36 demonstrated by Comfort et al. (2007) Biochemistry 46:3319-3330 (PMID: 17323919).

Relevant Links	CAZypedia InterPro PFAM PRINTS PROSITE

Statistics	CAZy Entries (202); GenBank/GenPept (345); Uniprot (72); 3D(6) : PDB(8); cryst(1);

Taxonomy	Bacteria(53); Eukaryota(149);

3D Display	GH27_3D


Bacteria
Protein	EC#	Organism	GenBank/GenPept		UniProt	PDB/3D
Acid345_1677	n.d.	Acidobacteria bacterium Ellin345	CP000360	ABF40678.1
Acid345_3249	n.d.	Acidobacteria bacterium Ellin345	CP000360	ABF42250.1
Amuc_1187	n.d.	Akkermansia muciniphila ATCC BAA-835	CP001071	ACD05012.1
isomaltodextranase (ImdA)	3.2.1.94	Arthrobacter dextranolyticus T6	AB117514	BAD34648.1
isomaltodextranase (G2d;Imd)	3.2.1.94	Arthrobacter globiformis T6	D30761 AB110787	BAA06424.1 BAC76886.2	Q44052 Q7WSN5	cryst
BH1870	n.d.	Bacillus halodurans C-125	NC_002570 AP001513	NP_242736.1 BAB05589.1	Q9KBQ5	3CC1	[B]
BF0227	n.d.	Bacteroides fragilis NCTC 9343 ATCC 25285 = NCTC 9343	CR626927	CAH06003.1
BF4189	n.d.	Bacteroides fragilis NCTC 9343 ATCC 25285 = NCTC 9343	CR626927	CAH09862.1
BF1418	n.d.	Bacteroides fragilis NCTC 9343 ATCC 25285 = NCTC 9343	CR626927	CAH07128.1
BF4391	n.d.	Bacteroides fragilis YCH46	AP006841	BAD51129.1
BF0271	n.d.	Bacteroides fragilis YCH46	AP006841	BAD47020.1
BF1485	n.d.	Bacteroides fragilis YCH46	AP006841	BAD48236.1
BT2662	n.d.	Bacteroides thetaiotaomicron VPI-5482	AE016937 NC_004663	AAO77769.1 NP_811575.1
BT3592	n.d.	Bacteroides thetaiotaomicron VPI-5482	AE016941 NC_004663	AAO78697.1 NP_812503.1
BT4157	n.d.	Bacteroides thetaiotaomicron VPI-5482	AE016944 NC_004663	AAO79262.1 NP_813068.1
BT3065	n.d.	Bacteroides thetaiotaomicron VPI-5482	AE016938 NC_004663	AAO78171.1 NP_811977.1
BT0065	n.d.	Bacteroides thetaiotaomicron VPI-5482	AE016926 NC_004663	AAO75172.1 NP_808978.1
BVU_0640	n.d.	Bacteroides vulgatus ATCC 8482	CP000139	ABR38348.1
BAD_1525	n.d.	Bifidobacterium adolescentis ATCC 15703	AP009256	BAF40306.1
BLD_1538 (GalA3)	n.d.	Bifidobacterium longum DJO10A	CP000605.1	ACD98983.1
BL0177	n.d.	Bifidobacterium longum NCC2705	AE014635 NC_004307	AAN24032.1 NP_695396.1
Caul_0313	n.d.	Caulobacter sp. K31	CP000927	ABZ69450.1
Caul_2129	n.d.	Caulobacter sp. K31	CP000927	ABZ71257.1
Caul_2412	n.d.	Caulobacter sp. K31	CP000927	ABZ71539.1
CJA_0246 - Aga27A	n.d.	Cellvibrio japonicus Ueda107	CP000934	ACE85287.1
-galactosidase 27A - Aga27A	n.d.	Cellvibrio mixtus	AY526725	AAS19696.1
galactosidase - Gal27A	n.d.	Clostridium cellulolyticum ATCC 35319	DQ778333	ABG76969.1
-galactosidase (AgaA) - Aga27A	3.2.1.22	Clostridium josui FERM P-9684	AB025362	BAB83765.1	Q8VV86
Fjoh_4947	n.d.	Flavobacterium johnsoniae UW101	CP000685	ABQ07946.1
Fjoh_3110	n.d.	Flavobacterium johnsoniae UW101	CP000685	ABQ06127.1
Fjoh_4436	n.d.	Flavobacterium johnsoniae UW101	CP000685	ABQ07440.1
FRAAL4014	n.d.	Frankia alni ACN14a	CT573213	CAJ62656.1
Haur_3892	n.d.	Herpetosiphon aurantiacus ATCC 23779	CP000875	ABX06526.1
Krad_3464	n.d.	Kineococcus radiotolerans SRS30216	CP000750	ABS04927.1
MAB_4303	n.d.	Mycobacterium abscessus	CU458896	CAM64376.1
Oter_3220	n.d.	Opitutus terrae PB90-1	CP001032	ACB76500.1
Oter_2301	n.d.	Opitutus terrae PB90-1	CP001032	ACB75583.1
Oter_1363	n.d.	Opitutus terrae PB90-1	CP001032	ACB74648.1
PGN_2032	n.d.	Porphyromonas gingivalis ATCC 33277	AP009380	BAG34550.1
PG0085	n.d.	Porphyromonas gingivalis W83	AE017172 NC_002950	AAQ65333.1 NP_904434.1
Sde_1593 - Agl27A	n.d.	Saccharophagus degradans 2-40	CP000282	ABD80855.1
-galactosidase (MelA;AgaB2;SACE_3139)	3.2.1.22	Saccharopolyspora erythraea NRRL 2338	AF061331 AM420293	AAC99325.1 CAM02417.1	Q9Z4N5
SACE_7065	n.d.	Saccharopolyspora erythraea NRRL 2338	AM420293	CAM06226.1
Acid_1588	n.d.	Solibacter usitatus Ellin6076	CP000473	ABJ82579.1
Acid_0705	n.d.	Solibacter usitatus Ellin6076	CP000473	ABJ81706.1
Acid_2820	n.d.	Solibacter usitatus Ellin6076	CP000473	ABJ83806.1
Acid_1472	n.d.	Solibacter usitatus Ellin6076	CP000473	ABJ82464.1
dextranase (Dex1)	n.d.	Sphingobacterium sp. V-54	AB244722	BAE92747.1	Q1XGE4
SAV1077	n.d.	Streptomyces avermitilis MA-4680	AP005025 NC_003155	BAC68787.1 NP_822252.1
SAV2186 (AgaA2)	n.d.	Streptomyces avermitilis MA-4680	AP005029 NC_003155	BAC69897.1 NP_823362.1
SAV1475 (AgaB2)	n.d.	Streptomyces avermitilis MA-4680	AP005027 NC_003155	BAC69185.1 NP_822650.1
SAV628 (AgaA1)	n.d.	Streptomyces avermitilis MA-4680	AP005023 NC_003155	BAC68338.1 NP_821803.1
SCO0284 or SCF85.12	n.d.	Streptomyces coelicolor A3(2)	AL939104 NC_003888	CAB54169.1 NP_624613.1	Q9S2C9


Eukaryota
Protein	EC#	Organism	GenBank/GenPept		UniProt	PDB/3D
-N-acetylgalactosaminidase (NagA)	3.2.1.49	Acremonium sp. No.413	AB037976	BAB08149.1	Q9HFB1
galactan:galactan galactosyltransferase 1 (GGT-1)	2.4.1.-	Ajuga reptans	AY386246	AAR02007.1
At5g08370/F8L15_100	n.d.	Arabidopsis thaliana	AY090238 AY085529 BT000619 - AL392174 NM_120921	AAL90902.1 AAM62753.1 AAN18186.1 ABI00031.1 CAC08337.1 NP_568193.1	Q8RX86 Q9FT98
At3g26380/F20C19.10	n.d.	Arabidopsis thaliana	AP001298 NC_003074	BAB02198.1 NP_189269.1	Q9LIN8
At3g56310/F18O21_270	n.d.	Arabidopsis thaliana	AY074321 AY114020 AL163763 NM_115489	AAL67017.1 AAM45068.1 CAB87430.1 NP_191190.2	Q9LYL2 Q8VXZ7
At5g08380/F8L15_110	n.d.	Arabidopsis thaliana	AY093200 BT008497 AL392174 NM_120922	AAM13199.1 AAP37856.1 CAC08338.1 NP_196455.1	Q8RWB9 Q9FT97
-galactosidase A (AglA;AFUA_5G13830;Afu5g13830)	n.d.	Aspergillus fumigatus Af293	AAHF01000003	EAL91233.1
AFUA_6G02560 (Afu6g02560)	n.d.	Aspergillus fumigatus Af293	AAHF01000012	EAL85784.1
-galactosidase B (AglB)	3.2.1.22	Aspergillus fumigatus IMI 385708
AN7624.2	n.d.	Aspergillus nidulans FGSC A4	DQ490512 AACD01000130	ABF50888.1 EAA61810.1
-galactosidase (AN7152.2)	3.2.1.22	Aspergillus nidulans FGSC A4	AACD01000122 DQ490505	EAA61404.1 ABF50881.1
AN0022.2 (fragment)	n.d.	Aspergillus nidulans FGSC A4	AACD01000002	EAA65341.1
-galactosidase II (AglB;An02g11150) - Gal27B	3.2.1.22	Aspergillus niger 513.88	AM270028	CAK44445.1
-galactosidase B (AglB)	3.2.1.22	Aspergillus niger CBS 120.49 / N400	Y18586	CAB46229.1	Q9Y865
An06g00170 (AglA)	3.2.1.22	Aspergillus niger CBS 513.88	AM270108	CAK44933.1
An11g06330	n.d.	Aspergillus niger CBS 513.88	AM270241	CAK96935.1
An01g01320	n.d.	Aspergillus niger CBS 513.88	AM269954	CAK43504.1
An14g01800	n.d.	Aspergillus niger CBS 513.88	AM270315	CAK46492.1
-galactosidase A (Ag1A;AglA)	3.2.1.22	Aspergillus niger N402	X63348	AAE69559.1 CAA44950.1	P28351
AO090003001305	n.d.	Aspergillus oryzae RIB 40	AP007155	BAE58368.1
AO090023000151	n.d.	Aspergillus oryzae RIB 40	AP007157	BAE58728.1
AO090005000217	n.d.	Aspergillus oryzae RIB 40	AP007151	BAE55309.1
-galactosidase A (Gla) (fragment)	n.d.	Ateles belzebuth chamek	AF106699	AAD16964.1	O97898
-N-acetylgalactosaminidase	n.d.	Bombyx mori	DQ311273	ABD36218.1
-N-acetylgalactosaminidase (MGC140188;NagA)	n.d.	Bos taurus	BT021618 BC114813	AAX46465.1 AAI14814.1
R07B7.11	n.d.	Caenorhabditis elegans Bristol N2	Z75955 NM_073630	CAB00120.1 NP_506031.1	Q21801
-galactosidase (Pag2)	n.d.	Carica papaya	AY253299	AAP04002.1	Q84QC9
-galactosidase (fragment)	n.d.	Cerastium arcticum	EF127845	ABN13605.1
-galactosidase	3.2.1.22	Coffea arabica	L27992 -	AAA33022.1 AAQ77888.1	Q42656
-galactosidase (Gal1)	3.2.1.22	Coffea arabica Caturra rojo T2308	AJ877911	CAI47559.1	Q5DUH8
-galactosidase (Gal1)	3.2.1.22	Coffea canephora 97	AJ877912	CAI47560.1
acid -galactosidase 2	3.2.1.22	Cucumis sativus	DQ361277	ABC88435.1
acid -galactosidase 1	n.d.	Cucumis sativus	DQ320569	ABC55266.1
Sequence 175 from patent US 7071380 (fragment)	n.d.	Cucurbita spp	-	ABH99998.1
-galactosidase	3.2.1.22	Cyamopsis tetragonoloba	M35837 X14619	AAA72859.1 AAE69558.1 CAA32772.1	P14749
Unknown (protein for MGC:123333) (fragment)	n.d.	Danio rerio	BC108078	AAI08079.1
Unknown (protein for MGC:101584) (probable fragment)	n.d.	Danio rerio	BC083209	AAH83209.1
Zgc:123333 protein	n.d.	Danio rerio	BC139643	AAI39644.1
DEHA0F28490g	n.d.	Debaryomyces hansenii CBS767	CR382138	CAG89932.1
CG7997	n.d.	Drosophila melanogaster	AE003807 AY069427 AE003807 NM_137275 NM_166170	AAF58008.2 AAL39572.1 AAM68519.1 NP_611119.1 NP_725571.1	Q9V7N9 Q8T0B5
CG5731	n.d.	Drosophila melanogaster	AE003627 AY113489 NM_135510	AAF52871.2 AAM29494.1 NP_609354.1	Q9VL27 Q8MYY3
N-acetyl--galactosaminidase	3.2.1.49	Gallus gallus	L18754 -	AAA16614.1 AAE69554.1	Q90744	1KTB 1KTC	[A] [A]
-galactosidase (fragment)	3.2.1.22	Ganoderma lucidum
-galactosidase	3.2.1.22	Glycine max	U12926 -	AAA73963.1 AAR65332.1	Q39811
-galactosidase (GLA)	n.d.	Helianthus annuus	AB092594	BAC66445.1	Q84VQ7
ORF (fragment)	n.d.	Heterocapsa triquetra	AY826915	AAW79376.1
N-acetyl--galactosaminidase / -galactosidase B (bK250D10.5;NagA)	3.2.1.49 3.2.1.22	Homo sapiens	M38083 M62783 M29276 M59199 - BC000095 Z99716 CR456527 NM_000262	AAA36351.1 AAA51677.1 AAA59902.1 AAB06718.1 AAE69555.1 AAH00095.1 CAB41237.1 CAG30413.1 NP_000253.1	P17050
-galactosidase A (GLA)	3.2.1.22	Homo sapiens	M13571 M18242 M20317 U78027 - - BC002689 - BT006864 - - - - - - - - - - - - - - - - - - - - - - - - - - - D00039 AK222627 AK291095 AK297148 X05790 X14448 AL035422 AX030659 AX840234 NM_000169	AAA51676.1 AAA52514.1 AAA52559.1 AAB64203.1 AAE66433.1 AAE69556.1 AAH02689.1 AAN92813.1 AAP35510.1 AAQ31818.1 AAQ68716.1 AAQ81221.1 AAY03282.1 AAY71885.1 AAY71886.1 AAY71887.1 AAY71888.1 AAY71889.1 AAY71890.1 AAY71891.1 AAY71892.1 AAY71893.1 AAY71894.1 AAY72531.1 AAY72532.1 AAY72533.1