CAZy ~ Carbohydrate-Active enZymes


   
Glycoside Hydrolase Family 13
  
Known Activities-amylase (EC 3.2.1.1); pullulanase (EC 3.2.1.41); cyclomaltodextrin glucanotransferase (EC 2.4.1.19); cyclomaltodextrinase (EC 3.2.1.54); trehalose-6-phosphate hydrolase (EC 3.2.1.93); oligo--glucosidase (EC 3.2.1.10); maltogenic amylase (EC 3.2.1.133); neopullulanase (EC 3.2.1.135); -glucosidase (EC 3.2.1.20); maltotetraose-forming -amylase (EC 3.2.1.60); isoamylase (EC 3.2.1.68); glucodextranase (EC 3.2.1.70); maltohexaose-forming -amylase (EC 3.2.1.98); maltotriose-forming -amylase (EC 3.2.1.116); branching enzyme (EC 2.4.1.18); trehalose synthase (EC 5.4.99.16); 4--glucanotransferase (EC 2.4.1.25); maltopentaose-forming -amylase (EC 3.2.1.-) ; amylosucrase (EC 2.4.1.4) ; sucrose phosphorylase (EC 2.4.1.7); malto-oligosyltrehalose trehalohydrolase (EC 3.2.1.141); isomaltulose synthase (EC 5.4.99.11); amino acid transporter.
 
MechanismRetaining
 
Catalytic Nucleophile/BaseAsp (experimental)
 
Catalytic Proton DonorGlu (experimental)
 
3D Structure StatusFold: ( / ) 8
 
ClanGH-H
 
NoteNew: many members have been assigned to subfamilies as described by Stam et al. (2006) Protein Eng Des Sel. 19, 555–562 (PMID: 17085431)
 
Relevant LinksAAMY   EBI Protein of the Month   HOMSTRAD   InterPro   PDB Molecule of the Month   PFAM   PRINTS   
 
StatisticsCAZy Entries (5221);  GenBank/GenPept (7448);  Uniprot (1162);  3D(66) : PDB(284); cryst(6);
 
TaxonomyArchaea( 4 / 79 );  Bacteria( 46 / 3810 );  Eukaryota( 12 / 1230 );  unclassified( 0 / 18 );  
 
Regular DisplayGH13
 
        
        Archaea          
ProteinEC#OrganismGenBank/GenPeptUniProtPDB/3DSubf
-amylase (AmyA) 3.2.1.1
Pyrococcus woesei AAD54338.1
AAF44693.1
ACH26211.1
Q9P9L0
1MWO
1MXD
1MXG
A
A
A
7
maltooligosyltrehalose synthase (TreY) 5.4.99.15
Sulfolobus acidocaldarius BAA11009.1
BAA11865.1
Q53688
1IV8
A
26
-amylase / maltooligosyltrehalose trehalohydrolase 3.2.1.1

3.2.1.141		Sulfolobus solfataricus KM1BAA11010.1
AAR46454.1
Q55088
1EH9
1EHA
A
A
10
isoamylase / 4--glucanotransferase (TreX;SSO2094;GlgX;ORF-c06_004;c06021) 2.4.1.25

3.2.1.68		Sulfolobus solfataricus P2 CAA69504.1
CAC23738.1
AAK42273.1
NP_343483.1
P95868
2VNC
2VR5
2VUY
A
A
A
11
        
        Bacteria          
ProteinEC#OrganismGenBank/GenPeptUniProtPDB/3DSubf
-amylase (Amy1;BAA) 3.2.1.1
Bacillus amyloliquefaciens CAA23430.1
AAA22191.1
AAA22192.1
CAA01489.1
AAE12381.1
AAE13947.1
AAE37101.1
CAC16484.1
AAE59727.1
AAE62349.1
CAC88551.1
CAD19035.1
CAD20678.1
CAD26702.1
AAN20003.1
AAN27445.1
AAN99911.1
CAD67517.1
CAD88112.1
AAP02211.1
AAR63623.1
AAR76030.1
AAS26291.1
AAY72173.1
ABJ43095.1
ABJ43099.1
ABN29369.1
ACK07344.1
P00692
3BH4
A
5
oligo-1,6-glucosidase (MalL) 3.2.1.10
Bacillus cereus ATCC 7064CAA37583.1
P21332
1UOK
A
 
cyclodextrin glucanotransferase (Cgt) 2.4.1.19
Bacillus circulans 251CAA55023.1
P43379
1CDG
1CGV
1CGW
1CGX
1CGY
1CXE
1CXF
1CXH
1CXI
1CXK
1CXL
1D3C
1DTU
1EO5
1EO7
1KCK
1KCL
1OT1
1OT2
1PEZ
1PJ9
1TCM
2CXG
2DIJ
A
A
A
A
A
A
A
A
A
A
A
A
A
A
A
A
A
A
A
A
A
B
A
A
2
cyclodextrin glucanotransferase 2.4.1.19
Bacillus circulans 8CAA48401.1
P30920
1CGT
1CGU
3CGT
4CGT
5CGT
6CGT
7CGT
8CGT
9CGT
A
A
A
A
A
A
A
A
A
2
cyclodextrin glucanotransferase (Cgt) 2.4.1.19
Bacillus clarkii 7364BAB91217.1
CAD87625.1
ABC06412.1
BAB91217.2
Q8L3E0
cryst 2
-amylase 3.2.1.1
Bacillus halmapalus AAE12377.1
AAE59724.1
AAE59730.1
AAE62346.1
AAE62352.1
CAC88548.1
CAD19032.1
CAD20675.1
CAD26699.1
CAD67514.1
AAP02208.1
AAP02214.1
AAS26288.1
AAS26294.1
AAY06017.1
AAY72170.1
AAY72176.1
ACK07341.1
 1W9X
2GJP
2GJR
A
A
A
5
-amylase (AmyA;AmyS;BLA) 3.2.1.1
Bacillus licheniformis CAA26981.1
AAA22193.1
AAA22226.1
AAA22240.1
CAA01355.1
CAA03075.1
CAC16483.1
CAC88550.1
CAD19034.1
CAD20677.1
CAD26701.1
CAD26706.1
AAN99913.1
AAO26743.1
CAD88111.1
AAT46561.1
P06278
1BLI
1BPL
1BPL
1OB0
1VJS
A
A
B
A
A
5
alkaline -amylase (AmyK) 3.2.1.1
Bacillus sp. KSM-1378BAA32431.1
CAD35985.1
CAD38135.1
AAN99906.1
AAR63625.1
AAR68734.1
AAV01045.1
ABE15847.1
ABI05897.1
ABY03536.1
O82839
2DIE
A
5
cyclodextrin glucanotransferase (Cgt) 2.4.1.19
Bacillus sp. 1011 AAA22308.1
P05618
1D7F
1DED
1I75
1PAM
1UKQ
1UKS
1UKT
1V3J
1V3K
1V3L
1V3M
A
A
A
A
A
A
A
A
A
A
A
2
maltohexaose-forming amylase 3.2.1.98
Bacillus sp. 707 AAA22231.1
AAE59728.1
AAE62350.1
AAP02212.1
AAR63620.1
AAS26292.1
AAY06019.1
AAY72174.1
ACK07346.1
P19571
1WP6
1WPC
2D3L
2D3N
A
A
A
A
5
cyclomaltodextrinase (CDI5;BspCMD) 3.2.1.54
Bacillus sp. I-5 AAA92925.1
Q59226
1EA9
C
20
-amylase (Kra2) (fragment)  

Bacillus sp. KR-8104 ACD93218.1
ACD93218.2
 3DC0
A
28
-amylase (AmyK38) 3.2.1.1
Bacillus sp. KSM-K38 CAC39917.1
BAB71820.1
CAD35986.1
CAD38133.1
AAN18958.1
AAV01046.1
CAJ00040.1
CAJ28078.1
ABA16539.1
ABI05896.1
ABY03537.1
Q93I48
1UD2
1UD3
1UD4
1UD5
1UD6
1UD8
A
A
A
A
A
A
5
-amylase 3.2.1.1
Bacillus subtilis 2633 / NATTOCAA30643.1
AAA22194.1
ABW34932.1
Q45520
Q45516
1BAG
1UA7
A
A
28
pullulanase type I / glycogen-debranching enzyme (AmyX;BSU29930) 2.4.1.25

3.2.1.33

3.2.1.41		Bacillus subtilis subsp. subtilis str. 168 AAC00283.1
CAB14971.1
NP_390871.1
ACH14984.1
O34587
2E8Y
2E8Z
2E9B
B
B
A
14
sucrose phosphorylase (SucP) 2.4.1.7
Bifidobacterium adolescentis DSM 20083AAO33821.1
Q84HQ2
1R7A
2GDU
2GDV
A
A
A
18
malto-oligosyltrehalose trehalohydrolase (DR0464) 3.2.1.141
Deinococcus radiodurans R1 AAF10042.1
NP_294187.1
Q9RX51
2BHU
2BHY
2BHZ
2BXY
2BXZ
2BY0
2BY1
2BY2
2BY3
A
A
A
A
A
A
A
A
A
10
branching-enzyme (GlgB;b3432) 2.4.1.18
Escherichia coli K-12 AAA23872.1
AAA58230.1
AAC76457.1
NP_417890.1
CAP11381.1
ABX94236.1
P07762
1M7X
A
9
pullulanase I (PulA) 3.2.1.41
Fervidobacterium pennivorans VEN5AAD30387.1
Q9XDB5
cryst 14
cyclomaltodextrinase (Cdase;FspCMD) 3.2.1.54
Flavobacterium sp. 92 CAD32957.1
Q8KKG0
3EDD
3EDE
3EDF
3EDJ
3EDK





 
-glucosidase (Gsj) 3.2.1.20
Geobacillus sp. HTA-462 BAE48285.1
 2ZE0
A
 
maltogenic -amylase (Novamyl) 3.2.1.133
Geobacillus stearothermophilus C599AAA22233.1
AAE62848.1
AAE62849.1
AAY11155.1
ABA38666.1
CAL60144.1
P19531
1QHO
1QHP
A
A
2
cyclomaltodextrinase / neopullulanase (NplT;BsCMD) 3.2.1.135

3.2.1.54		Geobacillus stearothermophilus TRS40AAA22622.1
P38940
1J0H
1J0I
1J0J
1J0K
A
A
A
B
20
-amylase (AmyS) 3.2.1.1
Geobacillus stearothermophilus DY5 / PHI300 / NZ-3AAA22227.1
AAA22241.1
CAA26547.1
AAA22235.2
AAE59725.1
AAE62347.1
AAP02209.1
AAS26289.1
AAY06018.1
AAY72171.1
P06279
1HVX
A
5
cyclodextrin glucanotransferase (Cgt) 2.4.1.19
Geobacillus stearothermophilus NO. 2CAA41770.1
CAA41772.1
CAA41771.1
P31797
1CYG
A
2
-amylase B (AmyB) 3.2.1.1
Halothermothrix orenii ACB11224.1
 3BC9
3BCD
3BCF
A
A
A
 
-amylase A (AmyA) 3.2.1.1
Halothermothrix orenii AAN52525.1
Q8GPL8
1Q76
1WML
1WZA


A
36
pullulanase (PulA) 3.2.1.41
Klebsiella aerogenes W70AAA25124.1
AAR52613.1
P07811
2FGZ
2FH6
2FH8
2FHB
2FHC
2FHF
A
A
A
A
A
A
13
isomaltulose synthase (PalI) 5.4.99.11
Klebsiella sp. LX3 AAK82938.1
CAF32992.1
CAF32993.1
Q8KR84
1M53
A
 
lp_0179 (Amy2)  

Lactobacillus plantarum WCFS1 CAD62849.1
NP_784011.1
 3DHU
A
 
amylosucrase (Ams) 2.4.1.4
Neisseria polysaccharea CAA03058.1
CAA03083.1
CAA09772.1
AAT15258.1
AAW13710.1
ACK26675.1
Q9ZEU2
1G5A
1JG9
1JGI
1MVY
1MW0
1MW1
1MW2
1MW3
1S46
1ZS2
A
A
A
A
A
A
A
A
A
A
4
cyclodextrin glucanotransferase (CgtM) 2.4.1.19
Paenibacillus macerans IAM1243CAA41773.1
AAA22298.1
AAC04359.1
P04830
O52766
cryst 2
multidomain amylase 3.2.1.1
Paenibacillus polymyxa CAA68344.1
AAA85446.1
P21543
cryst  
-amylase (Amy) 3.2.1.1
Pseudoalteromonas haloplanktis TAB23 / A23CAA41481.1
P29957
1AQH
1AQM
1B0I
1G94
1G9H
1JD7
1JD9
1KXH
1L0P
A
A
A
A
A
A
A
A
A
 
isoamylase (Iam) 3.2.1.68
Pseudomonas amyloderamosa SB-15CAA31754.1
AAA25854.1
P10342
1BF2
A
11
sucrose isomerase (trehalulose synthase) (MutB) 5.4.99.11
Pseudomonas mesoacidophila MX-45CAF32995.1
AAY69574.1
ABC33903.1
 1ZJA
1ZJB
2PWD
2PWE
2PWF
2PWG
2PWH
A
A
A
A
A
A
A
 
maltotetraose-forming amylase (AmyP) 3.2.1.60
Pseudomonas stutzeri MO-19AAA25707.1
AAA25709.1
ABN31444.1
ABN31445.1
ABN31446.1
ABN31447.1
ABN31448.1
ABN31449.1
ABN31450.1
ABN31451.1
ABN31452.1
P13507
1AMG
1GCY
1JDA
1JDC
1JDD
1QI3
1QI4
1QI5
1QPK
2AMG

A
A
A
A
A
A
A
A
A
 
dextran -1,6-glucosidase (DexB) 3.2.1.70
Streptococcus mutans ATCC 25175BAE79634.1
 2ZIC
2ZID
2ZID
A
A
A
31
cyclomaltodextrinase / neopullulanase (TVA II;TvCMD) 3.2.1.135

3.2.1.54		Thermoactinomyces vulgaris R-47BAA02473.1
BAA97040.1
BAB40638.1
Q08751
1BVZ
1G1Y
1JF5
1JF6
1JI2
1JIB
1JL8
1VB9
1VFK
1VFM
1VFO
1VFU
1WZK
1WZL
1WZM
2D2O
A
A
A
B
B
B
B
A
A
A
A
A
A
A
A
A
20
-amylase I (TVA I) 3.2.1.1
Thermoactinomyces vulgaris R-47BAA02471.1
Q60053
1JI1
1UH2
1UH3
1UH4
2D0F
2D0G
2D0H
A
A
A
A
A
A
A
 
cyclodextrin glucanotransferase (AmyA) 2.4.1.19
Thermoanaerobacterium thermosulfurigenes EM1AAB00845.1
P26827
1A47
1CIU
3BMV
3BMW
A
A
A
A
2
4--glucanotransferase (Mgt;TM0364) 2.4.1.25
Thermotoga maritima MSB8 CAA90693.1
AAD35451.1
NP_228175.1
Q60035
P80099
1LWH
1LWJ
B
A
 
4--glucanotransferase (MmtA) 2.4.1.25
Thermotoga maritima MSB8 (DSM 3109)CAA04525.1
O33838
1GJU
1GJW
A
A
 
cyclomaltodextrinase / maltogenic -amylase (ThCMD;ThMA) 3.2.1.133

3.2.1.54		Thermus sp. IM6501 AAC15072.1
O69007
1GVI
1SMA
A
A
20
TTHA1563  

Thermus thermophilus HB8 BAD71386.1
 2Z1K
A
20
sucrose hydrolase (Suh) 3.2.1.-
Xanthomonas axonopodis pv. glycines 8RAAAQ93678.1
 3CZE
3CZG
3CZK
3CZL
A
A
A
A
4
        
        Eukaryota          
ProteinEC#OrganismGenBank/GenPeptUniProtPDB/3DSubf
-amylase 3.2.1.1
Aspergillus niger  P56271
2AAA
A
1
-amylase A / B (AmyA;AmyB;An05g02100) 3.2.1.1
Aspergillus niger CBS 513.88 CAL33800.1
CAK44871.1
 2GUY
2GVY
A
A
1
-amylase (AmyB;TAA;Amy1;Amy2;Amy3;Asp o 21;Aspo21) 3.2.1.1
Aspergillus oryzae DSM63303AAA32708.1
BAA00336.1
CAA31218.1
CAA31220.1
CAA31219.1
CAC40348.1
CAT03377.1
P10529
2TAA
6TAA
7TAA
A
A
A
1
-amylase (pancreatic) (Amy2A) 3.2.1.1
Homo sapiens