CAZy ~ Carbohydrate-Active enZymes


   
Glycoside Hydrolase Family 13
  
Known Activities-amylase (EC 3.2.1.1); pullulanase (EC 3.2.1.41); cyclomaltodextrin glucanotransferase (EC 2.4.1.19); cyclomaltodextrinase (EC 3.2.1.54); trehalose-6-phosphate hydrolase (EC 3.2.1.93); oligo--glucosidase (EC 3.2.1.10); maltogenic amylase (EC 3.2.1.133); neopullulanase (EC 3.2.1.135); -glucosidase (EC 3.2.1.20); maltotetraose-forming -amylase (EC 3.2.1.60); isoamylase (EC 3.2.1.68); glucodextranase (EC 3.2.1.70); maltohexaose-forming -amylase (EC 3.2.1.98); maltotriose-forming -amylase (EC 3.2.1.116); branching enzyme (EC 2.4.1.18); trehalose synthase (EC 5.4.99.16); 4--glucanotransferase (EC 2.4.1.25); maltopentaose-forming -amylase (EC 3.2.1.-) ; amylosucrase (EC 2.4.1.4) ; sucrose phosphorylase (EC 2.4.1.7); malto-oligosyltrehalose trehalohydrolase (EC 3.2.1.141); isomaltulose synthase (EC 5.4.99.11); amino acid transporter.
 
MechanismRetaining
 
Catalytic Nucleophile/BaseAsp (experimental)
 
Catalytic Proton DonorGlu (experimental)
 
3D Structure StatusFold: ( / ) 8
 
ClanGH-H
 
NoteNew: many members have been assigned to subfamilies as described by Stam et al. (2006) Protein Eng Des Sel. 19, 555–562 (PMID: 17085431)
 
Relevant LinksAAMY   EBI Protein of the Month   HOMSTRAD   InterPro   PDB Molecule of the Month   PFAM   PRINTS   
 
StatisticsCAZy Entries (4789);  GenBank/GenPept (6936);  Swissprot (1176);  3D(54) : PDB(268); cryst(4);
 
TaxonomyArchaea(76);  Bacteria(3517);  Eukaryota(1178);  unclassified(18);  
 
3D DisplayGH13_3D
 
         
        Archaea          
ProteinEC#OrganismGenBank/GenPeptUniProtPDB/3DSubf
pullulanase (ApuA) 3.2.1.41
Desulfurococcus mucosusAF247191
AAG31033.1
Q9HHB0
   
-amylase (AmyH) 3.2.1.1
Haloarcula hispanicaAJ890146
CAI64586.1
    
rrnAC0224 (AglA1) n.d.Haloarcula marismortui ATCC 43049AY596297
AAV45283.1
    
rrnAC1962 (MalA) n.d.Haloarcula marismortui ATCC 43049AY596297
AAV46830.1
    
rrnAC2350 (Amy) n.d.Haloarcula marismortui ATCC 43049AY596297
AAV47170.1
    
-amylase (Amy) n.d.Haloferax mediterraneiAJ876899
CAI46245.1
   2
HQ2540A n.d.Haloquadratum walsbyi strain: DSM 16790 = HBSQ001AM180088
CAJ52652.1
    
HQ2031A n.d.Haloquadratum walsbyi strain: DSM 16790 = HBSQ001AM180088
CAJ52158.1
    
maltooligosyltrehalose trehalohydrolase (TreZ) 3.2.1.141
Metallosphaera hakonensis JCM 8857AY444509
AAR18372.1
   10
glycogen debranching enzyme (TreX) n.d.Metallosphaera hakonensis JCM 8857AY510703
AAS00512.1
   11
maltooligosyltrehalose synthase (TreY) 5.4.99.15
Metallosphaera hakonensis JCM 8857AY444508
AAR18371.1
   26
Memar_1265 n.d.Methanoculleus marisnigri JR1CP000562
ABN57195.1
   9
Memar_0386 n.d.Methanoculleus marisnigri JR1CP000562
ABN56319.1
    
MA1641 n.d.Methanosarcina acetivorans C2AAE010836
NC_003552
AAM05050.1
NP_616570.1
Q8TQA8
   
TreX n.d.Methanosarcina acetivorans C2AAE010884
NC_003552
AAM05403.1
NP_616923.1
Q8TPB3
  11
MA3032 n.d.Methanosarcina acetivorans C2AAE011005
NC_003552
AAM06405.1
NP_617925.1
Q8TLK4
   
Mbar_A2517 n.d.Methanosarcina barkeri str. fusaroCP000099
AAZ71430.1
   11
Mbar_A1790 n.d.Methanosarcina barkeri str. fusaroCP000099
AAZ70731.1
    
maltotriose-forming -amylase 3.2.1.116

3.2.1.1
Natronococcus amylolyticus Ah-36D26510
BAA05516.1
Q60224
   
PTO0067 n.d.Picrophilus torridus DSM 9790AE017261
AAT42652.1
   9
PTO0068 n.d.Picrophilus torridus DSM 9790AE017261
AAT42653.1
   3
PTO0069 n.d.Picrophilus torridus DSM 9790AE017261
AAT42654.1
   16
PAE0318 n.d.Pyrobaculum aerophilum IM2AE009762
NC_003364
AAL62712.1
NP_558530.1
Q8ZZC9
   
Pars_2149 n.d.Pyrobaculum arsenaticum DSM 13514CP000660
ABP51695.1
    
Pcal_0222 n.d.Pyrobaculum calidifontis JCM 11548CP000561
ABO07659.1
    
Pisl_1967 n.d.Pyrobaculum islandicum DSM 4184CP000504
ABL89114.1
    
-amylase (AmyA;PF0477) 3.2.1.1
Pyrococcus furiosus DSM 3638U96622
AF001268
AE010170
NC_003413
AAB67705.1
AAC45663.1
AAL80601.1
NP_578206.1
O08452
Q8U3I9
  7
-amylase / cyclomaltodextrinase (PFTA;PF1939) - Amy133.2.1.1

3.2.1.54
Pyrococcus furiosus DSM 3638AE010288
NC_003413
AAL82063.1
NP_579668.1
Q8TZP8
   
cyclodextrin glucanotransferase (PfCgt;PF0478;PF0478*) 2.4.1.19
Pyrococcus furiosus DSM 3638AE010170
-
-
DQ192528
NC_003413
AAL80602.1
ABA30024.1
ABA30025.1
ABA33720.1
NP_578207.1
Q8U3I8
  2
-amylase (AmyA) 3.2.1.1
Pyrococcus woeseiAF177906
AF240464
AAD54338.1
AAF44693.1
Q9P9L0
1MWO
1MXD
1MXG
[A]
[A]
[A]
7
Smar_0613 n.d.Staphylothermus marinus F1CP000575
ABN69720.1
    
maltooligosyltrehalose synthase (TreY) 5.4.99.15
Sulfolobus acidocaldariusD64129
D83245
BAA11009.1
BAA11865.1
Q53688
O08280
O08065
1IV8
[A]
26
-amylase/maltooligosyltrehalose trehalohydrolase (TreZ) 3.2.1.1

3.2.1.141
Sulfolobus acidocaldariusD64131
D83245
BAA11011.1
BAA11863.1
Q53641
O08279
O08064
  10
isoamylase (TreX) 3.2.1.68
Sulfolobus acidocaldarius ATCC33909D83245
BAA11864.1
O05152
  11
Saci_1440 (TreZ) n.d.Sulfolobus acidocaldarius DSM 639CP000077
AAY80766.1
   10
Saci_1439 n.d.Sulfolobus acidocaldarius DSM 639CP000077
AAY80765.1
   11
TreX (fragment) n.d.Sulfolobus islandicus B9BAY247907
AAP93396.1
    
TreX (fragment) n.d.Sulfolobus islandicus D4AY247906
AAP93395.1
    
TreX (fragment) n.d.Sulfolobus islandicus I7AY247909
AAP93398.1
    
TreX (fragment) n.d.Sulfolobus islandicus K10BAY247905
AAP93394.1
    
TreX (fragment) n.d.Sulfolobus islandicus N15BAY247910
AAP93399.1
    
TreX (fragment) n.d.Sulfolobus islandicus N23AAY247902
AAP93391.1
    
TreX (fragment) n.d.Sulfolobus islandicus N38DAY247911
AAP93400.1
    
TreX (fragment) n.d.Sulfolobus islandicus Ren1H1AY247908
AAP93397.1
    
TreX (fragment) n.d.Sulfolobus islandicus U3CAY247903
AAP93392.1
    
TreX (fragment) n.d.Sulfolobus islandicus V8AY247904
AAP93393.1
    
maltooligosyl trehalose trehalohydrolase (TreZ) 3.2.1.141
Sulfolobus shibataeAF201335
AY121427
AAF17553.1
AAM81589.1
Q9UWN9
  10
maltooligosyltrehalose synthase (TreY) 5.4.99.15
Sulfolobus shibataeAF201336
AY121427
AAF17554.1
AAM81591.1
Q9UWN8
  26
glycogen debranching enzyme (TreX) n.d.Sulfolobus shibatae B12AY121427
AAM81590.1
Q8NKQ3
  11
-amylase / maltooligosyltrehalose trehalohydrolase 3.2.1.1

3.2.1.141
Sulfolobus solfataricus KM1-
D64130
AAR46454.1
BAA11010.1
Q55088
1EH9
1EHA
[A]
[A]
10
glycosyltrehalose-producing enzyme 5.4.99.15
Sulfolobus solfataricus KM1D64128
BAA11008.1
Q55262
  26
isoamylase / 4--glucanotransferase (TreX;SSO2094;GlgX;ORF-c06_004;c06021) 3.2.1.68

2.4.1.25
Sulfolobus solfataricus P2AE006815
Y08256
AL512975
NC_002754
AAK42273.1
CAA69504.1
CAC23738.1
NP_343483.1
P95868
  11
maltooligosyltrehalose synthase (TreY;SSO2095) 5.4.99.15
Sulfolobus solfataricus P2AE006815
Y08256
NC_002754
AAK42274.1
CAA69505.1
NP_343484.1
P95869
  26
maltooligosyltrehalose trehalohydrolase (TreZ;SSO2093;c06020) 3.2.1.141
Sulfolobus solfataricus P2AE006815
Y08256
AL512975
NC_002754
AAK42272.1
CAA69503.1
CAC23737.1
NP_343482.1
P95867
  10
ST0928 n.d.Sulfolobus tokodaii str. 7AP000984
NC_003106
BAB65940.1
NP_376831.1
Q973H3
  11
ST0929 n.d.Sulfolobus tokodaii str. 7AP000984
NC_003106
BAB65941.1
NP_376832.1
Q973H2
  26
ST0926 + ST0927 n.d.Sulfolobus tokodaii str. 7AP000984
AP000984
NC_003106
NC_003106
BAB65938.1
BAB65939.1
NP_376829.1
NP_376830.1
Q973H5
Q973H4
   
pullulan hydrolase type III (PulhA) 3.2.1.-
Thermococcus aggregansAJ251532
CAB94218.1
Q9P9A0
   
-amylase (Amy) 3.2.1.1
Thermococcus hydrothermalis AL662AF068255
AAC97877.1
O93647
  7
-amylase (Amy) (fragment) n.d.Thermococcus hydrothermalis AL662AF068256
AAC97878.1
O93648
  7
TK0977 n.d.Thermococcus kodakaraensis KOD1AP006878
BAD85166.1
    
TK1770 n.d.Thermococcus kodakaraensis KOD1AP006878
BAD85959.1
    
-amylase (ApkA;TK1884) 3.2.1.1
Thermococcus kodakaraensis KOD1D83793
AP006878
BAA21130.1
BAD86073.1
O33476
  7
cyclodextrin glucanotransferase (TK-cgt) (TK2172) n.d.Thermococcus kodakaraensis KOD1AB072372
AP006878
BAB78538.1
BAD86361.1
Q8X268
  2
-amylase (BD5031) 3.2.1.1
Thermococcus sp. AEPII 1aAF504063
AAM48113.1
Q8NKR4
  7
cyclomaltodextrinase (CdnA) 3.2.1.54
Thermococcus sp. B1001AB034969
BAB18100.1
Q9HHC8
   
cyclodextrin glucanotransferase (CgtA) 2.4.1.19
Thermococcus sp. B1001AB025721
AB034969
BAA88217.1
BAB18101.1
Q9UWN2
  2
-amylase (BD5064) 3.2.1.1
Thermococcus sp. GU5L5AF504062
AAM48112.1
Q8NKR5
  7
-amylase n.d.Thermococcus sp. NA1DQ144136
ABA26948.1
   7
-amylase (Amy) 3.2.1.1
Thermococcus sp. OGL-20PAY608688
AAT11125.1
   7
-amylase 3.2.1.1
Thermococcus sp. Rt3AF017454
AAB87860.1
O50200
  7
Tpen_1454 n.d.Thermofilum pendens Hrk 5CP000505
ABL78851.1
   2
Tpen_1458 n.d.Thermofilum pendens Hrk 5CP000505
ABL78855.1
    
Ta0138 n.d.Thermoplasma acidophilum DSM 1728AL445063
NC_002578
CAC11285.1
NP_393616.1
Q9HLT7
  20
maltogenic -amylase (TpMA;TVG0226058) 3.2.1.133
Thermoplasma volcanium GSS1AP000991
NC_002689
BAB59359.1
NP_110736.1
Q97C86
  20
ORF (fragment) n.d.uncultured haloarchaeonEF583998
ABQ76050.1
    
 
         
        Bacteria          
ProteinEC#OrganismGenBank/GenPeptUniProtPDB/3DSubf
AM1_2293 n.d.Acaryochloris marina MBIC11017CP000828
ABW27303.1
   5
AM1_0622 n.d.Acaryochloris marina MBIC11017CP000828
ABW25671.1
   20
AM1_3649 n.d.Acaryochloris marina MBIC11017CP000828
ABW28639.1
   18
AM1_3468 n.d.Acaryochloris marina MBIC11017CP000828
ABW28460.1
   4
AM1_0996 n.d.Acaryochloris marina MBIC11017CP000828
ABW26038.1
   9
AM1_0142 n.d.Acaryochloris marina MBIC11017CP000828
ABW25228.1
    
ACL_0448 n.d.Acholeplasma laidlawii PG-8ACP000896
ABX81068.1
    
ACL_0667 n.d.Acholeplasma laidlawii PG-8ACP000896
ABX81283.1
   36
ACL_0658 n.d.Acholeplasma laidlawii PG-8ACP000896
ABX81274.1
    
ACL_0656 n.d.Acholeplasma laidlawii PG-8ACP000896
ABX81272.1
    
ACL_0529 n.d.Acholeplasma laidlawii PG-8ACP000896
ABX81147.1
   9
ACL_0665 n.d.Acholeplasma laidlawii PG-8ACP000896
ABX81281.1
   14
ACL_0657 n.d.Acholeplasma laidlawii PG-8ACP000896
ABX81273.1
   20
ACL_0531 n.d.Acholeplasma laidlawii PG-8ACP000896
ABX81149.1
   14
Acry_2934 n.d.Acidiphilium cryptum JF-5CP000697
ABQ32124.1
   3
Acry_2936 n.d.Acidiphilium cryptum JF-5CP000697
ABQ32126.1
   9
Acry_2935 n.d.Acidiphilium cryptum JF-5CP000697
ABQ32125.1
   16
Acry_2937 n.d.Acidiphilium cryptum JF-5CP000697
ABQ32127.1
   11
Acid345_4079 n.d.Acidobacteria bacterium Ellin345CP000360
ABF43079.1
    
Acid345_1485 n.d.Acidobacteria bacterium Ellin345CP000360