GlycosylTransferase Family 104

Activities in FamilydTDP-β-L-Rhap : arginine α-L-rhamnosyltransferase (EC 2.4.1.-)
Mechanism Inverting
NoteLassak et al. identified EarP as EF-P arginine R32 specific rhamnosyl transferase in Shewanella oneidensis using dTDP-b-L-rhamnose as donor substrate (PMID=25686373). The results of this study were subsequently confirmed for Pseudomonas aeruginosa (PMID=26060278), Pseudomonas putida (PMID=28951478) and Neisseria meningitidis (PMID=26840407). In 2016, it has been shown for S. oneidensis (PMID=28451135) and P. aeruginosa (PMID=28451332) that the enzyme is an inverting glycosyltransferase, thus mediating the formation of an a-L-rhamnosidic linkage. A structural basis for the arginine glycosylation reaction was provided by Krafczyk, Macosek and coworkers by having solved the crystal structure of the GT-B folding EarP from P. putida and an accompanying biochemical analysis of the enzyme (PMID=28951478).
Statistics GenBank accession (983); Uniprot accession (0); PDB accession (1); 3D entries (1); cryst (0)
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Bacteria
Protein Name EC#OrganismGenBank UniprotPDB/3D
 RSM_02140   Ralstonia solanacearum M7 ASS42327.1    
 RSOE_CAD01655   Ralstonia solanacearum OE1-1 APC68107.1    
 RUN215_v1_1420046   Ralstonia solanacearum Phyl III-seqv23 CUV57617.1    
 PSS4_v1_150047   Ralstonia solanacearum Phyl III-seqv23 CUV16587.1    
 RUN1985_v1_1230009   Ralstonia solanacearum Phyl III-seqv23 CUV31870.1    
 TO10_v1_50046   Ralstonia solanacearum Phyl III-seqv23 CUV43409.1    
 RUN39_v1_670036   Ralstonia solanacearum Phyl III-seqv23 CUV13978.1    
 RD1301_v1_460033   Ralstonia solanacearum Phyl III-seqv23 CUV59577.1    
 RUN1744_v1_70009   Ralstonia solanacearum Phyl III-seqv23 CUV21624.1
CUV35165.1
CUV39491.1
   
 RSPO_c02296   Ralstonia solanacearum Po82 AEG69592.1    
 RPSI07_2322   Ralstonia solanacearum PSI07 CBJ51687.1    
 RSSE_c2327   Ralstonia solanacearum SEPPX05 ARU22739.1    
 UW163_11975   Ralstonia solanacearum UW163 AMP70138.1    
 RSUY_14890   Ralstonia solanacearum UY031 ALF87847.1    
 A3768_2513   Ralstonia solanacearum YC40-M ANH33655.1    
 ACH51_11545   Ralstonia solanacearum YC45 AKZ26908.1    
 RALSY_40210   Ralstonia syzygii R24 CCA86010.1    
 UC35_20085   Ramlibacter tataouinensis 5-10 AMO24715.1    
 Rta_24520   Ramlibacter tataouinensis TTB310 AEG93550.1    
 A4W93_16560   Rhizobacter gummiphilus NS21 ARN21378.1    
 RA876_11600   Rhodoferax antarcticus DSM 24876 APW46897.1    
 Rfer_2243   Rhodoferax ferrireducens T118 DSM 15236 ABD69965.1    
 RS694_08345   Rhodoferax saidenbachensis DSM 22694 APW42539.1    
 RD110_18910   Rhodoferax sp. DCY110 APW39022.1    
 RD2015_2110   Roseateles depolymerans KCTC 42856 ALV06585.1    
 RGE_16210   Rubrivivax gelatinosus IL144 BAL94962.1    
 Sama_1700   Shewanella amazonensis SB2B ABL99906.1    
 Sbal175_2279   Shewanella baltica BA175 AEG11532.1    
 Sbal117_2354   Shewanella baltica OS117 AEH14075.1    
 Sbal_2231   Shewanella baltica OS155 ABN61727.1
ABN63880.1
   
 Shew185_2140   Shewanella baltica OS185 ABS08282.1    
 Sbal195_2190   Shewanella baltica OS195 ABX49359.1    
 Sbal223_2244   Shewanella baltica OS223 ACK46743.1    
 Sbal678_2193   Shewanella baltica OS678 ADT94350.1    
 CF168_10050   Shewanella bicestrii JAB-1 ASK69185.1    
 Sden_1991   Shewanella denitrificans OS217 ABE55274.1    
 Sfri_1909   Shewanella frigidimarina NCIMB 400 ABI71755.1    
 Shal_2244   Shewanella halifaxensis HAW-EB4 ABZ76803.1    
 SJ2017_1983   Shewanella japonica KCTC 22435 ARD22284.1    
 Shew_1916   Shewanella loihica PV-4 ABO23782.1    
 CFF01_09770   Shewanella marisflavi EP1 ASJ96844.1    
 dTDP-β-L-Rhap : arginine α-L-rhamnosyltransferase (EarP; SO_2329) 2.4.1.- Shewanella oneidensis MR-1 AAN55363.1    
 Spea_2050   Shewanella pealeana ATCC 700345 ABV87370.1    
 swp_2617   Shewanella piezotolerans WP3 ACJ29353.1    
 Sps_00780   Shewanella psychrophila WP2 AQS35973.1    
 Sputcn32_1876   Shewanella putrefaciens CN-32 ABP75599.1    
 Ssed_2338   Shewanella sediminis HAW-EB3 ABV36947.1    
 Shewana3_2140   Shewanella sp. ANA-3 ABK48370.1    
 CEQ32_04255   Shewanella sp. FDAARGOS_354 ASF14318.1    
 Shewmr4_2037   Shewanella sp. MR-4 ABI39110.1    
 Shewmr7_1938   Shewanella sp. MR-7 ABI42927.1    
 Sputw3181_2132   Shewanella sp. W3-18-1 ABM24960.1    
 Swoo_2269   Shewanella woodyi ATCC 51908 ACA86550.1    
 Slit_2076   Sideroxydans lithotrophicus ES-1 ADE12304.1    
 M5M_15405   Simiduia agarivorans SA1 = DSM 21679 AFV00214.1    
 CAP31_10665   Sulfuriferula sp. AH1 ARU32095.1    
 SUTH_01567   Sulfuritalea hydrogenivorans sk43H DSM22779 BAO29363.1    
 CAV20_02875   Taylorella equigenitalis 09-0932 ASY40634.1    
 CA604_03045   Taylorella equigenitalis 15-1587 ASY39115.1    
 CA605_02870   Taylorella equigenitalis 89-0490 ASY37647.1    
 B9Z30_02945   Taylorella equigenitalis 92-0972 ASY30342.1    
 CA943_02880   Taylorella equigenitalis 98-0554 ASY42069.1    
 CN03_13400   Thalassolituus oleivorans K188 APR67836.1    
 TOL_0961   Thalassolituus oleivorans MIL-1 CCU71397.1    
 R615_12580   Thalassolituus oleivorans R6-15 AHK16417.1    
 AC731_014440   Thauera humireducens SgZ-1 AMO38030.1    
 CCZ27_20295   Thauera sp. K11 ATE61996.1    
 Tmz1t_0133   Thauera sp. MZ1T ACK52930.1    
 Tbd_0856   Thiobacillus denitrificans ATCC 25259 AAZ96809.1    
 THIAE_04725   Thiomicrospira aerophila AL3 AHF01186.1    
 Thicy_0842   Thiomicrospira cyclica ALM1 AEG31614.1    
 THI_1398   Thiomonas arsenitoxydans 3As CAZ88085.1    
 Tint_1106   Thiomonas intermedia K12 ADG30496.1    
 THICB2_640084   Thiomonas sp. CB2 CDW95228.1    
 Tola_3148   Tolumonas auensis DSM 9187 ACQ94736.1    
 Tresu_2078   Treponema succinifaciens DSM 2489 AEB14948.1    
 CKY39_12080   Variovorax boronicumulans J1 ATA53873.1    
 VAPA_1c18670   Variovorax paradoxus B4 AGU48975.1    
 Varpa_1955   Variovorax paradoxus EPS ADU36164.1    
 Vapar_1780   Variovorax paradoxus S110 ACS18430.1    
 VAR608DRAFT_6419   Variovorax sp. HW608 SCK59351.1    
 AX767_16780   Variovorax sp. PAMC 28711 AMM25824.1    
 VITFI_CDS2378   Vitreoscilla filiformis ATCC 15551 ASM78156.1    
 PSELUDRAFT_0064   Vogesella sp. LIG4 SCK05098.1    
 AZF00_11365   Zhongshania aliphaticivorans SM2 AMO68862.1    
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