GlycosylTransferase Family 104

Activities in FamilydTDP-β-L-Rhap : arginine α-L-rhamnosyltransferase (EC 2.4.1.-)
Mechanism Inverting
NoteLassak et al. identified EarP as EF-P arginine R32 specific rhamnosyl transferase in Shewanella oneidensis using dTDP-b-L-rhamnose as donor substrate (PMID=25686373). The results of this study were subsequently confirmed for Pseudomonas aeruginosa (PMID=26060278), Pseudomonas putida (PMID=28951478) and Neisseria meningitidis (PMID=26840407). In 2016, it has been shown for S. oneidensis (PMID=28451135) and P. aeruginosa (PMID=28451332) that the enzyme is an inverting glycosyltransferase, thus mediating the formation of an a-L-rhamnosidic linkage. A structural basis for the arginine glycosylation reaction was provided by Krafczyk, Macosek and coworkers by having solved the crystal structure of the GT-B folding EarP from P. putida and an accompanying biochemical analysis of the enzyme (PMID=28951478).
Statistics GenBank accession (1215); Uniprot accession (0); PDB accession (5); 3D entries (2); cryst (0)
All (1217) Bacteria (1217) Structure (2) Characterized (4)
| 1 | ... | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 |
Bacteria
Protein Name EC#OrganismGenBank UniprotPDB/3D
 PFLU_1713   Pseudomonas fluorescens SBW25 CAY47961.1    
 HZ99_27400   Pseudomonas fluorescens UK4 AIG05734.1    
 B6D87_06775   Pseudomonas fragi NMC25 ARQ73924.1    
 SAMN05216594_4484   Pseudomonas fragi NRRL B-727 SDU65971.1    
 AV641_06840   Pseudomonas fragi P121 AMB78799.1    
 PFAS1_01770   Pseudomonas frederiksbergensis AS1 APV38125.1    
 BLL42_22120   Pseudomonas frederiksbergensis ERDD5:01 APC18288.1    
 CNN82_21840   Pseudomonas frederiksbergensis KNU-15 ATE78925.1    
 Psefu_2676   Pseudomonas fulva 12-X AEF22641.1    
 AL527_06080 (EarP)   Pseudomonas fulva FDAARGOS_167 AVF54785.1    
 KF707C_23380   Pseudomonas furukawaii KF707 BAU74026.1    
 SAMN05216581_2912   Pseudomonas fuscovaginae LMG 2158 SEI15146.1    
 SAMN05216579_3431   Pseudomonas granadensis LMG 27940 SDT36204.1    
 SAMN05216580_2825   Pseudomonas guangdongensis CCTCC 2012022 SDU40780.1    
 PKB_3666   Pseudomonas knackmussii B13 CDF85005.1    
 SAMN04490189_4235   Pseudomonas koreensis BS3658 SDE07704.1    
 A8L59_18310   Pseudomonas koreensis CRS05-R5 ANH99276.1    
 AYO71_21815   Pseudomonas koreensis D26 AMT90052.1    
 PkP19E3_19425 (EarP)   Pseudomonas koreensis P19E3 AVX90340.1    
 SAMN04490190_2217   Pseudomonas libanensis BS2975 SDK89881.1    
 SAMN04490191_2806   Pseudomonas lini BS3782 SDS97286.1    
 CLM75_08345   Pseudomonas lurida MYb11 AVJ37357.1    
 OU5_1552   Pseudomonas mandelii JR-1 AHZ68631.1    
 SAMN04489801_0194   Pseudomonas mandelii LMG 21607 SDT99533.1    
 SAMN05216476_4618   Pseudomonas mediterranea DSM 16733 SDU71352.1    
 CO724_13450   Pseudomonas mendocina MAE1-K ATH82108.1    
 C7A17_22490 (EarP)   Pseudomonas mendocina NEB698 AVO55407.1    
 MDS_2596   Pseudomonas mendocina NK-01 AEB58627.1    
 DW68_011385   Pseudomonas mendocina S5.2 ALN19205.1    
 Pmen_2129   Pseudomonas mendocina ymp ABP84889.1    
 AL532_27640 (EarP)   Pseudomonas monteilii FDAARGOS_171 AVH39804.1    
 X969_05425   Pseudomonas monteilii SB3078 AHC81444.1    
 X970_05400   Pseudomonas monteilii SB3101 AHC86873.1    
 APT63_14500   Pseudomonas monteilii USDA-ARS-USMARC-56711 AMA46727.1    
 SAMN04490196_0617   Pseudomonas moraviensis BS3668 SDU13119.1    
 CLJ08_05165   Pseudomonas mosselii BS011 ATB64027.1    
 O165_003015   Pseudomonas mosselii SJ10 AIN57324.1    
 SAMN05216202_2763   Pseudomonas mucidolens LMG 2223 SDU99023.1    
 NCTC8068_01825   Pseudomonas mucidolens NCTC8068 SQH32855.1    
 SAMN04490197_5581   Pseudomonas orientalis BS2775 SDU38984.1    
 BOP93_08120   Pseudomonas orientalis F9 AUZ45569.1    
 SAMN05216221_0749   Pseudomonas oryzae KCTC 32247 SDR95827.1    
 CYL20_26205   Pseudomonas palleroniana MAB3 AVE07904.1    
 NJ69_11410   Pseudomonas parafulva CRS01-1 AIZ33548.1    
 B2J77_06210   Pseudomonas parafulva PRS09-11288 AQW67852.1    
 RK21_03536   Pseudomonas plecoglossicida NyZ12 AJG15044.1    
 SAMN04490208_1524   Pseudomonas poae BS2776 SDN80561.1    
 H045_04965   Pseudomonas poae RE*1-1-14 AGE25069.1    
 SAMN05216296_1770   Pseudomonas pohangensis DSM 17875 SDU10109.1    
 SAMN05216222_4576   Pseudomonas prosekii LMG 26867 SDT47446.1    
 PPC_4366   Pseudomonas protegens Cab57 BAO63713.1    
 PFLCHA0_c43310   Pseudomonas protegens CHA0 AGL86090.1    
 CEP86_18200   Pseudomonas protegens FDAARGOS_307 ASE22313.1    
 H78_04564   Pseudomonas protegens H78 AQT11206.1    
 PFL_4265   Pseudomonas protegens Pf-5 AAY93521.1    
 PPSAL_1985   Pseudomonas pseudoalcaligenes CDR91212.1    
 BN5_2010   Pseudomonas pseudoalcaligenes CECT 5344 CECT5344 CDM40582.1    
 SAMN04490201_2142   Pseudomonas psychrophila BS3667 SDU50741.1    
 BJP27_03000   Pseudomonas psychrotolerans PRS08-11306 APQ10523.1    
 AWT69_001456   Pseudomonas putida 1A00316 AMK30093.1    
 KKK_18355   Pseudomonas putida B6-2 ANC82854.1    
 PPUBIRD1_3757   Pseudomonas putida BIRD-1 ADR61346.1    
 DW66_1781   Pseudomonas putida DLL-E4 AHZ76300.1    
 T1E_0932   Pseudomonas putida DOT-T1E AFO46790.1    
 CR511_07240   Pseudomonas putida E41 ATP43868.1    
 CR512_06980   Pseudomonas putida E46 ATP49123.1    
 Pput_3857   Pseudomonas putida F1 ABQ79981.1    
 PputGB1_1432   Pseudomonas putida GB-1 ABY97339.1    
 L483_06805   Pseudomonas putida H8234 AGN78283.1    
 B479_07225   Pseudomonas putida HB3267 PC9 AGA72361.1    
 IEC33019_0484   Pseudomonas putida IEC33019 ANY86085.1    
 Q5O_19325   Pseudomonas putida JB AOX10443.1    
 DKY63_11400 (EarP)   Pseudomonas putida JBC17 AWY40467.1    
 KF715C_ch15580   Pseudomonas putida KF715 BAW22131.1    
 dTDP-β-L-Rhap : arginine α-L-rhamnosyltransferase (EarP; PP_1857) 2.4.1.- Pseudomonas putida KT2440 AAN67476.1   5NV8[A]
 SAMN05216501_4882   Pseudomonas putida N1R SIS17489.1    
 PP4_39260   Pseudomonas putida NBRC 14164 BAN55779.1    
 YSA_02040   Pseudomonas putida ND6 AFK67873.1    
 AB688_20745   Pseudomonas putida PC2 ANC04399.1    
 BG030_07120   Pseudomonas putida PP112420 APE97831.1    
 RPPX_16740   Pseudomonas putida S12 AJA14938.1    
 N805_15300   Pseudomonas putida S13.1.2 AJQ48489.1    
 PPS_1494   Pseudomonas putida S16 AEJ12069.1    
 CWR53_06620   Pseudomonas putida SGAir0191 AUA32297.1    
 A210_19935   Pseudomonas putida SJTE-1 ANI04812.1    
 PputW619_1467   Pseudomonas putida W619 ACA71972.1    
 SAMN04490202_0909   Pseudomonas reinekei BS3776 SDO44405.1    
 PCA10_33620   Pseudomonas resinovorans NBRC 106553 BAN49094.1    
 LT40_01725   Pseudomonas rhizosphaerae DSM 16299 AIS16183.1    
 SAMN04490209_3256   Pseudomonas rhodesiae BS2777 SDV10305.1    
 PSPPH_3649   Pseudomonas savastanoi pv. phaseolicola 1448A AAZ37214.1    
 PSA3335_22135   Pseudomonas savastanoi pv. savastanoi NCPPB 3335 ARD14694.1    
 SAMN05216363_1171   Pseudomonas sihuiensis KCTC 32246 SDU78752.1    
 PMA3_07675   Pseudomonas silesiensis A3 ANJ55044.1    
 PS417_08475   Pseudomonas simiae WCS417 AIB35613.1    
 CXQ80_17060   Pseudomonas sp. 02C 26 AUF97412.1    
 CXQ81_23415   Pseudomonas sp. 09C 129 AUG03431.1    
 DJ564_23105 (EarP)   Pseudomonas sp. 31-12 AWM93465.1    
 SAMN05428955_5137   Pseudomonas sp. 7SR1 SIS27180.1    
 SAMN05216504_4567   Pseudomonas sp. A214 SIS11041.1    
Top


Last update: 2018-07-09 © Copyright 1998-2018
AFMB - CNRS - Université d'Aix-Marseille