GlycosylTransferase Family 104

Activities in FamilydTDP-β-L-Rhap : arginine α-L-rhamnosyltransferase (EC 2.4.1.-)
Mechanism Inverting
NoteLassak et al. identified EarP as EF-P arginine R32 specific rhamnosyl transferase in Shewanella oneidensis using dTDP-b-L-rhamnose as donor substrate (PMID=25686373). The results of this study were subsequently confirmed for Pseudomonas aeruginosa (PMID=26060278), Pseudomonas putida (PMID=28951478) and Neisseria meningitidis (PMID=26840407). In 2016, it has been shown for S. oneidensis (PMID=28451135) and P. aeruginosa (PMID=28451332) that the enzyme is an inverting glycosyltransferase, thus mediating the formation of an a-L-rhamnosidic linkage. A structural basis for the arginine glycosylation reaction was provided by Krafczyk, Macosek and coworkers by having solved the crystal structure of the GT-B folding EarP from P. putida and an accompanying biochemical analysis of the enzyme (PMID=28951478).
Statistics GenBank accession (983); Uniprot accession (0); PDB accession (1); 3D entries (1); cryst (0)
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Bacteria
Protein Name EC#OrganismGenBank UniprotPDB/3D
 JM49_09620   Pseudomonas chlororaphis subsp. aurantiaca JD37 AIS11933.1    
 PCAU_4264   Pseudomonas chlororaphis subsp. aurantiaca StFRB508 BAV76473.1    
 VM99_16010   Pseudomonas chlororaphis UFB2 AKJ99500.1    
 PCH70_37340 (fragment)   Pseudomonas cichorii JBC1 AHF68887.1    
 PcP3B5_20330   Pseudomonas citronellolis P3B5 AMO75483.1    
 A9C11_10075   Pseudomonas citronellolis SJTE-3 ANI14310.1    
 SAMN04490183_2848   Pseudomonas corrugata BS3649 SDU99756.1    
 AXG94_24025   Pseudomonas corrugata RM1-1-4 AOE64705.1    
 LK03_21225   Pseudomonas cremoricolorata AIR91619.1    
 PSEEN1561   Pseudomonas entomophila L48 CAK14423.1    
 SAMN05216591_2808   Pseudomonas extremaustralis DSM 17835 SDF38869.1    
 SAMN04490184_5263   Pseudomonas extremorientalis BS2774 SDP84921.1    
 PflA506_1761   Pseudomonas fluorescens A506 AFJ59796.1    
 SAMN04488487_4059   Pseudomonas fluorescens ATCC 13525 SNY11427.1    
 PSF113_1695   Pseudomonas fluorescens F113 AEV61719.1    
 AO356_03165   Pseudomonas fluorescens FW300-N2C3 ALI05820.1    
 TK06_12410   Pseudomonas fluorescens FW300-N2E2 AMZ71859.1    
 AO353_19075   Pseudomonas fluorescens FW300-N2E3 ALI03082.1    
 AYK59_13255   Pseudomonas fluorescens KENGFT3 AMS21069.1    
 A7319_08145   Pseudomonas fluorescens L111 AOE72794.1    
 A7318_07590   Pseudomonas fluorescens L228 AOE78460.1    
 A7317_08155   Pseudomonas fluorescens L321 AOE66979.1    
 VO64_4826   Pseudomonas fluorescens LBUM223 AKA85372.1    
 AK972_4111   Pseudomonas fluorescens LBUM636 AMW84911.1    
 B723_26320   Pseudomonas fluorescens NCIMB 11764 AKV09717.1    
 PF1751_v1c16820   Pseudomonas fluorescens PCL1751 AJP51385.1    
 Pfl01_4000   Pseudomonas fluorescens Pf0-1 ABA75737.1    
 PFLUOLIPICF7_28385   Pseudomonas fluorescens PICF7 AJZ96853.1    
 BH711_04000   Pseudomonas fluorescens Pt14 AOS73119.1    
 PFLU_1713   Pseudomonas fluorescens SBW25 CAY47961.1    
 HZ99_27400   Pseudomonas fluorescens UK4 AIG05734.1    
 B6D87_06775   Pseudomonas fragi NMC25 ARQ73924.1    
 SAMN05216594_4484   Pseudomonas fragi NRRL B-727 SDU65971.1    
 AV641_06840   Pseudomonas fragi P121 AMB78799.1    
 PFAS1_01770   Pseudomonas frederiksbergensis AS1 APV38125.1    
 BLL42_22120   Pseudomonas frederiksbergensis ERDD5:01 APC18288.1    
 CNN82_21840   Pseudomonas frederiksbergensis KNU-15 ATE78925.1    
 Psefu_2676   Pseudomonas fulva 12-X AEF22641.1    
 SAMN05216581_2912   Pseudomonas fuscovaginae LMG 2158 SEI15146.1    
 SAMN05216579_3431   Pseudomonas granadensis LMG 27940 SDT36204.1    
 SAMN05216580_2825   Pseudomonas guangdongensis CCTCC 2012022 SDU40780.1    
 PKB_3666   Pseudomonas knackmussii B13 CDF85005.1    
 SAMN04490189_4235   Pseudomonas koreensis BS3658 SDE07704.1    
 A8L59_18310   Pseudomonas koreensis CRS05-R5 ANH99276.1    
 AYO71_21815   Pseudomonas koreensis D26 AMT90052.1    
 SAMN04490190_2217   Pseudomonas libanensis BS2975 SDK89881.1    
 SAMN04490191_2806   Pseudomonas lini BS3782 SDS97286.1    
 OU5_1552   Pseudomonas mandelii JR-1 AHZ68631.1    
 SAMN04489801_0194   Pseudomonas mandelii LMG 21607 SDT99533.1    
 SAMN05216476_4618   Pseudomonas mediterranea DSM 16733 SDU71352.1    
 MDS_2596   Pseudomonas mendocina NK-01 AEB58627.1    
 DW68_011385   Pseudomonas mendocina S5.2 ALN19205.1    
 Pmen_2129   Pseudomonas mendocina ymp ABP84889.1    
 X969_05425   Pseudomonas monteilii SB3078 AHC81444.1    
 X970_05400   Pseudomonas monteilii SB3101 AHC86873.1    
 APT63_14500   Pseudomonas monteilii USDA-ARS-USMARC-56711 AMA46727.1    
 SAMN04490196_0617   Pseudomonas moraviensis BS3668 SDU13119.1    
 CLJ08_05165   Pseudomonas mosselii BS011 ATB64027.1    
 O165_003015   Pseudomonas mosselii SJ10 AIN57324.1    
 SAMN05216202_2763   Pseudomonas mucidolens LMG 2223 SDU99023.1    
 SAMN04490197_5581   Pseudomonas orientalis BS2775 SDU38984.1    
 SAMN05216221_0749   Pseudomonas oryzae KCTC 32247 SDR95827.1    
 NJ69_11410   Pseudomonas parafulva CRS01-1 AIZ33548.1    
 B2J77_06210   Pseudomonas parafulva PRS09-11288 AQW67852.1    
 RK21_03536   Pseudomonas plecoglossicida NyZ12 AJG15044.1    
 SAMN04490208_1524   Pseudomonas poae BS2776 SDN80561.1    
 H045_04965   Pseudomonas poae RE*1-1-14 AGE25069.1    
 SAMN05216296_1770   Pseudomonas pohangensis DSM 17875 SDU10109.1    
 SAMN05216222_4576   Pseudomonas prosekii LMG 26867 SDT47446.1    
 PPC_4366   Pseudomonas protegens Cab57 BAO63713.1    
 PFLCHA0_c43310   Pseudomonas protegens CHA0 AGL86090.1    
 CEP86_18200   Pseudomonas protegens FDAARGOS_307 ASE22313.1    
 H78_04564   Pseudomonas protegens H78 AQT11206.1    
 PFL_4265   Pseudomonas protegens Pf-5 AAY93521.1    
 PPSAL_1985   Pseudomonas pseudoalcaligenes CDR91212.1    
 BN5_2010   Pseudomonas pseudoalcaligenes CECT 5344 CECT5344 CDM40582.1    
 KF707C_23380   Pseudomonas pseudoalcaligenes KF707 = NBRC 110670 BAU74026.1    
 SAMN04490201_2142   Pseudomonas psychrophila BS3667 SDU50741.1    
 BJP27_03000   Pseudomonas psychrotolerans PRS08-11306 APQ10523.1    
 AWT69_001456   Pseudomonas putida 1A00316 AMK30093.1    
 KKK_18355   Pseudomonas putida B6-2 ANC82854.1    
 PPUBIRD1_3757   Pseudomonas putida BIRD-1 ADR61346.1    
 DW66_1781   Pseudomonas putida DLL-E4 AHZ76300.1    
 T1E_0932   Pseudomonas putida DOT-T1E AFO46790.1    
 Pput_3857   Pseudomonas putida F1 ABQ79981.1    
 PputGB1_1432   Pseudomonas putida GB-1 ABY97339.1    
 L483_06805   Pseudomonas putida H8234 AGN78283.1    
 B479_07225   Pseudomonas putida HB3267 PC9 AGA72361.1    
 IEC33019_0484   Pseudomonas putida IEC33019 ANY86085.1    
 Q5O_19325   Pseudomonas putida JB AOX10443.1    
 KF715C_ch15580   Pseudomonas putida KF715 BAW22131.1    
 dTDP-β-L-Rhap : arginine α-L-rhamnosyltransferase (EarP; PP_1857) 2.4.1.- Pseudomonas putida KT2440 SKB80791.1
AAN67476.1
  5NV8[A]
 SAMN05216501_4882   Pseudomonas putida N1R SIS17489.1    
 PP4_39260   Pseudomonas putida NBRC 14164 BAN55779.1    
 YSA_02040   Pseudomonas putida ND6 AFK67873.1    
 AB688_20745   Pseudomonas putida PC2 ANC04399.1    
 BG030_07120   Pseudomonas putida PP112420 APE97831.1    
 RPPX_16740   Pseudomonas putida S12 AJA14938.1    
 N805_15300   Pseudomonas putida S13.1.2 AJQ48489.1    
 PPS_1494   Pseudomonas putida S16 AEJ12069.1    
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