Glycoside Hydrolase Family 99

Activities in Familyglycoprotein endo-α-1,2-mannosidase (EC 3.2.1.130); mannan endo-1,2-α-mannanase (3.2.1.198)
Mechanism Retaining
3D Structure Status( β / α ) 8 barrel
Catalytic Nucleophile/BaseUnclear, possible neighboring group participation from O2 (after Thompson et al. Proc. Natl. Acad. Sci. USA., 2012, 109, 781-786)
Catalytic Proton DonorGlu (experimental)
NoteCreated after Spiro, Bhoyroo and Spiro (1997) J. Biol. Chem. 272, 29356-29363
External resourcesCAZypedia;
Statistics GenBank accession (238); Uniprot accession (7); PDB accession (40); 3D entries (3); cryst (0)
Bacteria
Protein Name EC#OrganismGenBankUniprot
PDB/3D Carbohydrate Ligands Resolution (Å)
 endo-α-1,2-mannosidase (BtGH99;BT3862) (BtGH99) 3.2.1.130
3.2.1.198
Bacteroides thetaiotaomicron VPI-5482 AAO78967.1
NP_812773.1
Q8A109
4ACY[A,B] 1.69
4ACZ[A,B] 1.99
4AD0[A,B,C,D] 2.09
 endo-α-1,2-mannosidase (BxGH99;EndoMan;BXY_34140) (BxGH99) 3.2.1.130
3.2.1.198
Bacteroides xylanisolvens XB1A CBK68409.1 D6D1V7
4AD1[A] 1.90
4AD2[A,B,C,D] α-D-Glcp-(1-1)-<non_carb>
<carb_like_IFM>
2.10
4AD3[A,B,C,D] α-D-Glcp-(1-1)-<non_carb>
<carb_like_DMJ>
2.00
4AD4[A] α-D-Glcp-(1-1)-<non_carb>
α-D-Manp-(1-2)-β-D-Manp
<carb_like_IFM>
1.90
4AD5[A] α-D-Glcp-(1-1)-<non_carb>
α-D-Manp-(1-2)-α-D-Manp
<carb_like_DMJ>
1.90
4UTF[A] α-D-Manp-(1-1)-<non_carb>
α-D-Manp-(1-2)-α-D-Manp
<carb_like_IFM>
1.30
4V27[A] α-D-Manp-(1-1)-<non_carb>
<carb_like_IFM>
1.90
4V28[A] α-D-Manp-(1-3)-α-D-Manp-(1-1)-<non_carb>
1.20
5LYR[A] α-D-Manp-(1-1)-<non_carb>
<carb_like_MNM>
1.14
5M03[A] α-D-Manp-(1-1)-<non_carb>
α-D-Manp-(1-2)-α-D-Manp
<carb_like_MNM>
1.05
5M17[A] α-D-Manp-(1-3)-D-1,2-deoxy-Glcp
1.03
5M3W[A] α-D-Manp-(1-2)-α-D-Manp
α-D-Manp-(1-3)-D-1,2-deoxy-Glcp
1.04
5M5D[A] α-D-Manp-(1-3)-D-1,2-deoxy-Glcp
1.07
5MC8[A] α-D-Manp-(1-2)-α-D-Manp
α-D-Manp-(1-3)-D-1,2-deoxy-Glcp
1.18
5MEL[A] α-D-Glcp-(1-1)-<non_carb>
1.20
6FAM[A] α-D-Manp-(1-1)-<non_carb>
1.13
6FAR[A] α-D-Manp-(1-1)-<non_carb>
<carb_like_MVL>
1.30
6FWG[A] α-D-Manp-(1-3)-α-D-Manp-(1-2)-α-D-Manp-(1-2)-α-D-Manp-(1-1)-methyl
1.07
6FWI[A] α-D-Glcp-(1-1)-<non_carb>
1.25
6FWJ[A] α-D-Glcp-(1-1)-<non_carb>
α-D-Manp-(1-2)-α-D-Manp
1.04
6FWL[A] α-D-Glcp-(1-1)-<non_carb>
1.12
6FWM[A] α-D-Glcp-(1-1)-<non_carb>
1.28
6FWO[A] α-D-Glcp-(1-1)-<non_carb>
1.34
6FWP[A] α-D-Manp-(1-2)-α-D-Manp
α-D-Manp-(1-3)-α-D-Manp
1.08
6FWQ[A] α-D-Manp-(1-2)-α-D-Manp
α-D-Manp-(1-3)-α-D-Manp
1.65
6HMG[A] α-D-Glcp-(1-1)-<non_carb>
1.27
6HMH[A] α-D-Glcp-(1-1)-<non_carb>
α-D-Manp-(1-2)-α-D-Manp
1.03
6ZJ6[AAA]
Eukaryota
Protein Name EC#OrganismGenBankUniprot
PDB/3D Carbohydrate Ligands Resolution (Å)
 glycoprotein endo-α-1,2-mannosidase (ManeA) 3.2.1.130 Homo sapiens CAE12165.1
AAI46672.1
AAI50200.1
AAL07306.1
AAL07307.1
AAQ75077.1
ACH33940.1
BAB14298.1
BAF84629.1
CAD97640.1
CAE45927.1
CAH33902.1
CAI17346.1
CAI17347.1
NP_078917.2
A6H8M6
Q5SRI9
Q8WWX4
6ZDC[A] 2.25
6ZDF[A,B,C] 3.00
6ZDK[AAA]
6ZDL[AAA]
6ZFA[AAA]
6ZFN[AAA]
6ZFQ[AAA]
6ZJ1[AAA]
6ZJ5[AAA]
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