Glycoside Hydrolase Family 99

Activities in Family	glycoprotein endo-α-1,2-mannosidase (EC 3.2.1.130); mannan endo-1,2-α-mannanase (3.2.1.198)
Mechanism	Retaining
3D Structure Status	( β / α ) ₈ barrel
Catalytic Nucleophile/Base	Unclear, possible neighboring group participation from O2 (after Thompson et al. Proc. Natl. Acad. Sci. USA., 2012, 109, 781-786)
Catalytic Proton Donor	Glu (experimental)
Note	Created after Spiro, Bhoyroo and Spiro (1997) J. Biol. Chem. 272, 29356-29363
External resources	CAZypedia;
Statistics	GenBank accession (430); Uniprot accession (7); PDB accession (40); 3D entries (3); cryst (0)


Bacteria
Protein Name	EC#	Reference	Organism	GenBank	Uniprot	PDB/3D
endo-α-1,2-mannosidase (BtGH99;BT3862) (BtGH99)	3.2.1.130 3.2.1.198	pubmed pubmed	Bacteroides thetaiotaomicron VPI-5482	AAO78967.1 NP_812773.1	Q8A109	4ACY[A,B] 4ACZ[A,B] 4AD0[A,B,C,D]
endo-α-1,2-mannosidase (BxGH99;EndoMan;BXY_34140) (BxGH99)	3.2.1.130 3.2.1.198	pubmed pubmed	Bacteroides xylanisolvens XB1A	CBK68409.1	D6D1V7	4AD1[A] 4AD2[A,B,C,D] 4AD3[A,B,C,D] 4AD4[A] 4AD5[A] 4UTF[A] 4V27[A] 4V28[A] 5LYR[A] 5M03[A] 5M17[A] 5M3W[A] 5M5D[A] 5MC8[A] 5MEL[A] 6FAM[A] 6FAR[A] 6FWG[A] 6FWI[A] 6FWJ[A] 6FWL[A] 6FWM[A] 6FWO[A] 6FWP[A] 6FWQ[A] 6HMG[A] 6HMH[A] 6ZJ6[AAA]
endo-α-1,2-mannosidase (Sama99;Sama_0297)	3.2.1.130	pubmed	Shewanella amazonensis SB2B	ABL98508.1	A1S2A2
Eukaryota
Protein Name	EC#	Reference	Organism	GenBank	Uniprot	PDB/3D
glycoprotein endo-α-1,2-mannosidase (ManeA)	3.2.1.130	pubmed	Homo sapiens	CAE12165.1 AAI46672.1 AAI50200.1 AAL07306.1 AAL07307.1 AAQ75077.1 ACH33940.1 BAB14298.1 BAF84629.1 CAD97640.1 CAE45927.1 CAH33902.1 CAI17346.1 CAI17347.1 NP_078917.2	A6H8M6 Q5SRI9 Q8WWX4	6ZDC[A] 6ZDF[A,B,C] 6ZDK[AAA] 6ZDL[AAA] 6ZFA[AAA] 6ZFN[AAA] 6ZFQ[AAA] 6ZJ1[AAA] 6ZJ5[AAA]
endo-α-1,2-mannosidase (Enman)	3.2.1.130	pubmed	Rattus norvegicus	AAT44962.1 AAB86925.1 NP_542963.1 XP_346734.1	Q5GF25
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