Glycoside Hydrolase Family 84

Activities in FamilyN-acetyl β-glucosaminidase (EC 3.2.1.52); hyaluronidase (EC 3.2.1.35); [protein]-3-O-(GlcNAc)-L-Ser/Thr β-N-acetylglucosaminidase (EC 3.2.1.169)
Mechanism Retaining
3D Structure Status( β / α ) 8
Catalytic Nucleophile/Basecarbonyl oxygen of C-2 acetamido group of substrate
Catalytic Proton DonorAsp
NoteMechanism shown in Macauley et al. (2005) JBC 280:25313-25322
External resourcesCAZypedia;
Commercial Enzyme Provider(s)PROZOMIX;
Statistics GenBank accession (691); Uniprot accession (107); PDB accession (63); 3D entries (6); cryst (0)
Bacteria
Protein Name EC#OrganismGenBankUniprot
PDB/3D Carbohydrate Ligands Resolution (Å)
 β-N-acetylglucosaminidase (BtOGA;BtGH84;BT4395;BT_4395) 3.2.1.169 Bacteroides thetaiotaomicron VPI-5482 AAO79500.1
NP_813306.1
Q89ZI2
2CHN[A,B] 1.95
2CHO[A,B] 1.85
2J47[A] <carb_like_GDV>
1.98
2J4G[A,B] 2.25
2JIW[A,B] 1.95
2VVN[A,B] 1.85
2VVS[A] β-D-Glcp1N2NAc-(1-1)-<non_carb>
2.24
2W4X[A] <non_carb>-(1-2)-β-D-GlcpN
2.42
2W66[A,B] <carb_like_HQ6>
2.27
2W67[A,B] <carb_like_F34>
2.25
2WCA[A] <non_carb>-(1-2)-β-D-Glcp1NN-(1-1)-<non_carb>
2.30
2WZH[A] 2.20
2WZI[A,B] 1.90
2X0H[A,B] <non_carb>-(1-2)-β-D-GlcpN-(1-1)-<non_carb>
2.21
2XJ7[A,B] <carb_like_GC2>
2.00
2XM1[A,B] <carb_like_LTM>
2.00
2XM2[A,B] β-D-Glcp1N2NAc-(1-1)-<non_carb>
1.95
4AIS[A,B] 2.00
4AIU[A] 2.25
4UR9[A,B] α-D-Glcp1N2NAc-(1-1)-<non_carb>
β-D-Glcp1N2NAc-(1-1)-<non_carb>
2.20
5ABE[A,B] 2.00
5ABF[A,B] 2.10
5ABG[A,B] 2.00
5ABH[A,B] 1.95
5FKY[A,B] 1.80
5FL0[A,B] 1.95
5FL1[A,B] 1.95
5MI4[A]
5MI5[A]
5MI6[A]
5MI7[A]
 hyaluronidase (NagJ;CpNagJ;CpOGA;CPF_1442) 3.2.1.52 Clostridium perfringens ATCC 13124 ABG84519.1 Q0TR53
2J62[A,B] <carb_like_GSZ>
2.26
2V5C[A,B] 2.10
2V5D[A] 3.30
2VUR[A,B] <non_carb>-(1-2)-β-D-GlcpN
2.20
2WB5[A,B] <carb_like_VGB>
2.31
2X0Y[A,B] 2.25
2YDQ[A] 2.60
2YDR[A] 2.75
2YDS[A] 2.55
4ZXL[A] 2.60
 β-N-acetylglucosaminidase (NagJ;CpNagJ;CPE1234) (GH84C) 3.2.1.52 Clostridium perfringens str. 13 BAB80940.1
NP_562150.1
Q8XL08
2CBI[A,B] 2.25
2CBJ[A,B] β-D-Glcp1N2NAc-(1-1)-<non_carb>
2.35
2XPK[A,B] 2.40
 OG2516_04129   Oceanicola granulosus HTCC2516 EAR51054.1 Q2CEE3
2XSA[A] 2.00
2XSB[A] α-D-GlcpNAc
2.11
 Tter_0116   Thermobaculum terrenum ATCC BAA-798 ACZ41038.1 D1CDN2
5DIY[A,B] 2.06
Eukaryota
Protein Name EC#OrganismGenBankUniprot
PDB/3D Carbohydrate Ligands Resolution (Å)
 O-glycoprotein β-N-acetylglucosaminidase / O-GlcNAcase (OGA;hOGA;Mea5;Mgea5;NCOAT) 3.2.1.169 Homo sapiens AAD05385.2
AAG21428.1
AAH39583.2
AAH47877.1
BAA31654.2
BAA31654.3
BAG63869.1
NP_036347.1
D3DR79
O60502
O75166
Q86WV0
Q9HAR0
5M7R[A,B] 2.35
5M7S[A,B] 2.40
5M7T[A,A,B,B] <carb_like_GDV>
2.60
5M7U[A,B] 2.30
5TKE[A,B] 2.48
5UHK[A,C] 2.97
5UHL[A,C] 3.14
5UHO[A,C] α-D-Glcp1N2NAc-(1-1)-<non_carb>
β-D-Glcp1N2NAc-(1-1)-<non_carb>
3.21
5UHP[A,B,C,D] 2.79
5UN8[A,B,C,D] 2.13
5UN9[A,B] 2.50
5VVO[A,B] 2.60
5VVT[A,C] 2.80
5VVU[A,C] 2.70
5VVV[A,C] 2.80
5VVX[A,C] 2.90
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