Activities in Family	[protein]-3-O-(GlcNAc)-L-Ser/Thr β-N-acetylglucosaminidase (EC 3.2.1.169); Hyaluronidase / hyaluronoglucosaminidase (EC 3.2.1.35); β-N-acetylhexosaminidase (EC 3.2.1.52);
Mechanism	Retaining e
3D Structure Status	( β / α ) 8 barrel
Catalytic Nucleophile/Base	carbonyl oxygen of C-2 acetamido group of substrate
Catalytic Proton Donor	Asp
Note	Mechanism shown in Macauley et al. (2005) JBC 280:25313-25322
External resources	CAZypedia;
Commercial Enzyme Provider(s)	PROZOMIX;
Statistics	GenBank accession (3308); Uniprot accession (25); PDB accession (78); 3D entries (10); cryst (0)

Bacteria
Protein Name	EC#	Organism	GenBank	Uniprot	PDB/3D Carbohydrate Ligands Resolution (Å)
β-N-acetylglucosaminidase (BtOGA;BtGH84;BT4395;BT_4395)	3.2.1.169	Bacteroides thetaiotaomicron VPI-5482	AAO79500.1 NP_813306.1	Q89ZI2	2CHN[A,B] 1.95 2CHO[A,B] 1.85 2J47[A] <carb_like_GDV> 1.98 2J4G[A,B] 2.25 2JIW[A,B] 1.95 2VVN[A,B] 1.85 2VVS[A] β-D-Glcp1N2NAc-(1-1)-<non_carb> 2.24 2W4X[A] <non_carb>-(1-2)-β-D-GlcpN 2.42 2W66[A,B] <carb_like_HQ6> 2.27 2W67[A,B] <carb_like_F34> 2.25 2WCA[A] <non_carb>-(1-2)-β-D-Glcp1NN-(1-1)-<non_carb> 2.30 2WZH[A] 2.20 2WZI[A,B] 1.90 2X0H[A,B] <non_carb>-(1-2)-β-D-GlcpN-(1-1)-<non_carb> 2.21 2XJ7[A,B] <carb_like_GC2> 2.00 2XM1[A,B] <carb_like_LTM> 2.00 2XM2[A,B] β-D-Glcp1N2NAc-(1-1)-<non_carb> 1.95 4AIS[A,B] 2.00 4AIU[A] 2.25 4UR9[A,B] α-D-Glcp1N2NAc-(1-1)-<non_carb> β-D-Glcp1N2NAc-(1-1)-<non_carb> 2.20 5ABE[A,B] 2.00 5ABF[A,B] 2.10 5ABG[A,B] 2.00 5ABH[A,B] 1.95 5FKY[A,B] 1.80 5FL0[A,B] 1.95 5FL1[A,B] 1.95 5MI4[A] 1.80 5MI5[A] β-D-Glcp1N2NAc-(1-1)-<non_carb> 2.15 5MI6[A] 2.00 5MI7[A] α-D-Glcp1N2NAc-(1-1)-<non_carb> 2.10 7K41[A] 2.00 7OU8[A,B]
exo-β-N-acetylglucosaminidase (NagH; CpGH84A; CPF_0184) (GH84A)	3.2.1.52	Clostridium perfringens ATCC 13124	ABG83307.1	A0A0H2YRL1 P26831 Q0TUP2	6PV4[A,B,C,D] 2.20
CPF_0875		Clostridium perfringens ATCC 13124	ABG84510.1		6PV5[A] 2.18
hyaluronidase (NagJ;CpNagJ;CpOGA;CPF_1442)	3.2.1.52 3.2.1.169	Clostridium perfringens ATCC 13124	ABG84519.1	Q0TR53	2J62[A,B] <carb_like_GSZ> 2.26 2V5C[A,B] 2.10 2V5D[A] 3.30 2VUR[A,B] <non_carb>-(1-2)-β-D-GlcpN 2.20 2WB5[A,B] <carb_like_VGB> 2.31 2X0Y[A,B] 2.25 2YDQ[A] 2.60 2YDR[A] 2.75 2YDS[A] 2.55 4ZXL[A] 2.60 5OXD[A] 2.60 6RHE[A] β-D-GlcpNAc-(1-0)-CYS 3.10 7KHV[A,B,C,D,E,F] 2.30
CPF_1487		Clostridium perfringens ATCC 13124	ABG84775.1		6PWI[A,B] 2.65
CMR01_07555		Clostridium perfringens LLY_N11	ATD48631.1		7KHV[F] 2.30
β-N-acetylglucosaminidase (NagJ;CpNagJ;CPE1234) (GH84C)	3.2.1.52	Clostridium perfringens str. 13	BAB80940.1 NP_562150.1	Q0TR53 Q8XL08	2CBI[A,B] 2.25 2CBJ[A,B] β-D-Glcp1N2NAc-(1-1)-<non_carb> 2.35 2XPK[A,B] 2.40
OG2516_04129		Oceanicola granulosus HTCC2516	EAR51054.1		2XSA[A] 2.00 2XSB[A] α-D-GlcpNAc 2.11 7KHS[A,B,C,D] 1.78
O-glycoprotein β-N-acetylglucosaminidase (TtOGA; Tter_0116)	3.2.1.169	Thermobaculum terrenum ATCC BAA-798	ACZ41038.1	D1CDN2	5DIY[A,B] 2.06
Eukaryota
Protein Name	EC#	Organism	GenBank	Uniprot	PDB/3D Carbohydrate Ligands Resolution (Å)
O-glycoprotein β-N-acetylglucosaminidase / O-GlcNAcase (OGA;hOGA;Mea5;Mgea5;NCOAT)	3.2.1.169	Homo sapiens	AAD05385.2 AAG21428.1 AAH39583.2 AAH47877.1 BAA31654.2 BAA31654.3 BAG63869.1 NP_036347.1	B4DYV7 D3DR79 O60502 O75166 Q86WV0 Q9HAR0	5M7R[A,B] 2.35 5M7S[A,B] 2.40 5M7T[A,B] <carb_like_GDV> 2.60 5M7U[A,B] 2.30 5TKE[A,B] 2.48 5UHK[A,C] 2.97 5UHL[A,C] 3.14 5UHO[A,C] α-D-Glcp1N2NAc-(1-1)-<non_carb> β-D-Glcp1N2NAc-(1-1)-<non_carb> 3.21 5UHP[A,B,C,D] 2.79 5UN8[A,B,C,D] 2.13 5UN9[A,B] 2.50 5VVO[A,B] 2.60 5VVT[A,C] 2.80 5VVU[A,C] 2.70 5VVV[A,C] 2.80 5VVX[A,C] 2.90 6HKI[A,B] 3.30 6PM9[A,B,C,D] 2.86 7OU6[A,B] 7YEH[C,D] β-D-GlcpNAc 3.92 8P0L[A,B] β-D-GlcpNAc-(1-0)-CYS 2.50
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