Glycoside Hydrolase Family 84

Activities in FamilyN-acetyl β-glucosaminidase (EC 3.2.1.52); hyaluronidase (EC 3.2.1.35); [protein]-3-O-(GlcNAc)-L-Ser/Thr β-N-acetylglucosaminidase (EC 3.2.1.169)
Mechanism Retaining
3D Structure Status( β / α ) 8 barrel
Catalytic Nucleophile/Basecarbonyl oxygen of C-2 acetamido group of substrate
Catalytic Proton DonorAsp
NoteMechanism shown in Macauley et al. (2005) JBC 280:25313-25322
External resourcesCAZypedia;
Commercial Enzyme Provider(s)PROZOMIX;
Statistics GenBank accession (1719); Uniprot accession (107); PDB accession (74); 3D entries (10); cryst (0)
All (1648) Archaea (3) Bacteria (1553) Eukaryota (88) unclassified (4) Structure (10) Characterized (14)
Bacteria
Protein Name EC#OrganismGenBankUniprot
PDB/3D Carbohydrate Ligands Resolution (Å)
 β-N-acetylglucosaminidase (BtOGA;BtGH84;BT4395;BT_4395) 3.2.1.169 Bacteroides thetaiotaomicron VPI-5482 AAO79500.1
NP_813306.1
Q89ZI2
2CHN[A,B] 1.02
2CHO[A,B] 1.90
2J47[A] <carb_like_GDV>
1.50
2J4G[A,B] 2.55
2JIW[A,B] 1.10
2VVN[A,B] 1.45
2VVS[A] β-D-Glcp1N2NAc-(1-1)-<non_carb>
1.90
2W4X[A] <non_carb>-(1-2)-β-D-GlcpN
2.10
2W66[A,B] <carb_like_HQ6>
1.37
2W67[A,B] <carb_like_F34>
2.00
2WCA[A] <non_carb>-(1-2)-β-D-Glcp1NN-(1-1)-<non_carb>
2.12
2WZH[A] 0.83
2WZI[A,B] 1.65
2X0H[A,B] <non_carb>-(1-2)-β-D-GlcpN-(1-1)-<non_carb>
2.60
2XJ7[A,B] <carb_like_GC2>
2.40
2XM1[A,B] <carb_like_LTM>
2.39
2XM2[A,B] β-D-Glcp1N2NAc-(1-1)-<non_carb>
1.60
4AIS[A,B] 1.60
4AIU[A] 2.20
4UR9[A,B] α-D-Glcp1N2NAc-(1-1)-<non_carb>
β-D-Glcp1N2NAc-(1-1)-<non_carb>
2.30
5ABE[A,B] 1.70
5ABF[A,B] 1.40
5ABG[A,B] 3.11
5ABH[A,B] 2.60
5FKY[A,B] 2.42
5FL0[A,B] 1.95
5FL1[A,B] 2.15
5MI4[A] 1.30
5MI5[A] β-D-Glcp1N2NAc-(1-1)-<non_carb>
1.70
5MI6[A] 2.40
5MI7[A] α-D-Glcp1N2NAc-(1-1)-<non_carb>
3.14
7K41[A] 1.70
 exo-β-N-acetylglucosaminidase (NagH;CpGH84A;CPF_0184) (GH84A) 3.2.1.52 Clostridium perfringens ATCC 13124 ABG83307.1 Q0TUP2
6PV4[A,B,C,D] 1.75
 CPF_0875   Clostridium perfringens ATCC 13124 ABG84510.1 Q0TSR6
6PV5[A] NMR
 hyaluronidase (NagJ;CpNagJ;CpOGA;CPF_1442) 3.2.1.52
3.2.1.169
Clostridium perfringens ATCC 13124 ABG84519.1 Q0TR53
2J62[A,B] <carb_like_GSZ>
1.70
2V5C[A,B] 1.86
2V5D[A] 1.65
2VUR[A,B] <non_carb>-(1-2)-β-D-GlcpN
1.25
2WB5[A,B] <carb_like_VGB>
2.50
2X0Y[A,B] 2.15
2YDQ[A] 1.43
2YDR[A] 1.70
2YDS[A] 2.14
4ZXL[A] 2.30
5OXD[A] 2.20
6RHE[A] β-D-GlcpNAc-(1-0)-CYS
1.74
7KHV[A] 1.77
 CPF_1487   Clostridium perfringens ATCC 13124 ABG84775.1 Q0TR10
6PWI[A,B] 1.5
 CMR01_07555   Clostridium perfringens LLY_N11 ATD48631.1  
7KHV[F] 1.77
 β-N-acetylglucosaminidase (NagJ;CpNagJ;CPE1234) (GH84C) 3.2.1.52 Clostridium perfringens str. 13 BAB80940.1
NP_562150.1
Q8XL08
2CBI[A,B] 1.86
2CBJ[A,B] β-D-Glcp1N2NAc-(1-1)-<non_carb>
2.70
2XPK[A,B] 2.30
 OG2516_04129   Oceanicola granulosus HTCC2516 EAR51054.1 Q2CEE3
2XSA[A] 1.17
2XSB[A] α-D-GlcpNAc
3.29
7KHS[A] 1.75
 Tter_0116   Thermobaculum terrenum ATCC BAA-798 ACZ41038.1 D1CDN2
5DIY[A,B] 1.85
Eukaryota
Protein Name EC#OrganismGenBankUniprot
PDB/3D Carbohydrate Ligands Resolution (Å)
 O-glycoprotein β-N-acetylglucosaminidase / O-GlcNAcase (OGA;hOGA;Mea5;Mgea5;NCOAT) 3.2.1.169 Homo sapiens AAD05385.2
AAG21428.1
AAH39583.2
AAH47877.1
BAA31654.2
BAA31654.3
BAG63869.1
NP_036347.1
D3DR79
O60502
O75166
Q86WV0
Q9HAR0
5M7R[A,B] 1.60
5M7S[A,B] 1.58
5M7T[A,B] <carb_like_GDV>
1.98
5M7U[A,B] 2.30
5TKE[A,B] 1.20
5UHK[A,C] 2.00
5UHL[A,C] 1.20
5UHO[A,C] α-D-Glcp1N2NAc-(1-1)-<non_carb>
β-D-Glcp1N2NAc-(1-1)-<non_carb>
2.57
5UHP[A,B,C,D] 1.75
5UN8[A,B,C,D] 2.00
5UN9[A,B] 2.3
5VVO[A,B] 1.80
5VVT[A,C] 1.65
5VVU[A,C] 2.38
5VVV[A,C] 1.90
5VVX[A,C] not applicable
6HKI[A,B] 2.30
6PM9[A,B,C,D] 2.10
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