Glycoside Hydrolase Family 84

Known Activities	N-acetyl β-glucosaminidase (EC 3.2.1.52); hyaluronidase (EC 3.2.1.35); [protein]-3-O-(GlcNAc)-L-Ser/Thr β-N-acetylglucosaminidase (EC 3.2.1.169)
Mechanism	Retaining
3D Structure Status	( β / α ) ₈
Catalytic Nucleophile/Base	carbonyl oxygen of C-2 acetamido group of substrate
Catalytic Proton Donor	Asp
Note	Mechanism shown in Macauley et al. (2005) JBC 280:25313-25322
External resources	CAZypedia;
Commercial Enzyme Provider(s)	PROZOMIX;
Statistics	GenBank accession (182); Uniprot accession (99); PDB accession (28); 3D entries (4); cryst (0)

Summary

All (133) Archaea (2) Bacteria (115) Eukaryota (15) unclassified (1) Structure (4 ) Characterized (10)

Bacteria
Protein Name	EC#	Organism	GenBank	Uniprot	PDB/3D
β-N-acetylglucosaminidase (BtGH84;BT4395;BT_4395)	3.2.1.169	Bacteroides thetaiotaomicron VPI-5482	AAO79500.1 NP_813306.1		2CHN[A,B] 2CHO[A,B] 2J47[A] 2J4G[A,B] 2JIW[A,B] 2VVN[A,B] 2VVS[A] 2W4X[A] 2W66[A,B] 2W67[A,B] 2WCA[A] 2WZH[A] 2WZI[A,B] 2X0H[A,B] 2XJ7[A,B] 2XM1[A,B] 2XM2[A,B]
β-N-acetylglucosaminidase (Nag84A)	3.2.1.52	Clostridium paraputrificum M-21	BAC99989.1	Q76D19
hyaluronidase (NagJ;CPF_1442)	3.2.1.52	Clostridium perfringens ATCC 13124	ABG84519.1	Q0TR53	2J62[A,B] 2V5C[A,B] 2V5D[A] 2VUR[A,B] 2WB5[A,B] 2X0Y[A,B]
hyaluronidase (NagH)	3.2.1.35	Clostridium perfringens CPN50	AAA23259.1	P26831.2
hyaluronidase / Î¼-toxin (NagH;CPE0191) (GH84A)	3.2.1.35	Clostridium perfringens str. 13	BAB79897.1 NP_561107.1
β-N-acetylglucosaminidase (NagJ;CPE1234) (GH84C)	3.2.1.52	Clostridium perfringens str. 13	BAB80940.1 NP_562150.1	Q8XL08	2CBI[A,B] 2CBJ[A,B] 2XPK[A,B]
O-glycoprotein β-N-acetylglucosaminidase (SPy1600)	3.2.1.169	Streptococcus pyogenes M1 GAS SF370	AAK34378.1 NP_269657.1	Q99YP8
Eukaryota
Protein Name	EC#	Organism	GenBank	Uniprot	PDB/3D
O-glycoprotein β-N-acetylglucosaminidase / O-GlcNAcase (OGA;Mea5;Mgea5;NCOAT)	3.2.1.169	Homo sapiens	AAD05385.2 AAG21428.1 AAH39583.2 AAH47877.1 BAA31654.2 BAG63869.1 NP_036347.1	D3DR79 O60502 O75166 Q86WV0 Q9HAR0
O-GlcNAcase (Mgea5;NCOAT)	3.2.1.169	Mus musculus	AAG43273.2 AAH20093.1 AAH41109.1 AAH54821.1 BAB28416.1 BAB31029.1 BAC40564.1 BAC97998.1 BAE26311.1 NP_076288.1	Q8BTT2 Q8CFX2 Q9CSJ4 Q9CU60 Q9EQQ9
O-GlcNAcase (Mgea5;NCOAT)	3.2.1.169	Rattus norvegicus	AAK72103.1 NP_571979.1	Q8VIJ5

Footnotes

Experimental characterization exclusively covers enzyme activities at present. If you are aware of any missing experimental characterization that should be mentioned here, we encourage you to send your comment and support of the experimental evidence, including an electronic copy or appropriate link to the support material (i.e., article(s), thesis, database, etc), to cazy@afmb.univ-mrs.fr.